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Article ; Online: Low-Cost, Real-Time Polymerase Chain Reaction System with Integrated RNA Extraction.

Kadja, Tchamie / Sun, Yvonne / Chodavarapu, Vamsy P

2023 Volume 23, Issue 10

Abstract: Rapid, easy-to-use, and low-cost systems for biological sample testing are important for point-of-care diagnostics and various other health applications. The recent pandemic of Coronavirus Disease 2019 (COVID-19) caused by the Severe Acute Respiratory ... ...

Abstract	Rapid, easy-to-use, and low-cost systems for biological sample testing are important for point-of-care diagnostics and various other health applications. The recent pandemic of Coronavirus Disease 2019 (COVID-19) caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) showed an urgent need to rapidly and accurately identify the genetic material of SARS-CoV-2, an enveloped ribonucleic acid (RNA) virus, in upper respiratory specimens from people. In general, sensitive testing methods require genetic material extraction from the specimen. Unfortunately, current commercially available extraction kits are expensive and involve time-consuming and laborious extraction procedures. To overcome the difficulties associated with common extraction methods, we propose a simple enzymatic assay for the nucleic acid extraction step using heat mediation to improve the polymerase chain reaction (PCR) reaction sensitivity. Our protocol was tested on Human Coronavirus 229E (HCoV-229E) as an example, which comes from the large
MeSH term(s)	Animals ; Humans ; Real-Time Polymerase Chain Reaction ; RNA ; COVID-19/diagnosis ; SARS-CoV-2/genetics ; Nucleic Acids ; Mammals ; COVID-19 Testing
Chemical Substances	RNA (63231-63-0) ; Nucleic Acids
Language	English
Publishing date	2023-05-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s23104604
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Learning Through Teaching: How Physicians Learn Medicine in Authentic Clinical Contexts.

Frija-Gruman, Nissim Maxim / Steinert, Yvonne / Macdonald, Mary Ellen / Sun, Ning-Zi

Academic medicine : journal of the Association of American Medical Colleges

2024

Abstract: Purpose: Little is known about the clinical knowledge and skills that are acquired by physicians through teaching, how such learning occurs, or the factors that influence this process. This study explored how physicians acquire clinical knowledge and ... ...

Abstract	Purpose: Little is known about the clinical knowledge and skills that are acquired by physicians through teaching, how such learning occurs, or the factors that influence this process. This study explored how physicians acquire clinical knowledge and skills through clinical teaching and examined the contextual elements that influence this learning. Method: Two theoretical frameworks informed this interpretive description study: situated learning and cognitive apprenticeship. From March to November 2021, semistructured interviews and follow-up discussions were conducted at McGill University with clinician-teachers who regularly supervise internal medicine residents. Participants were asked to describe how they learned clinical medicine through spontaneous clinical teaching, guided by questions relating to what they learned, memorable teaching moments, and factors influencing this learning. Data were analyzed iteratively, using both a deductive and inductive approach. Results: Of the 87 contacted physicians, 45 responded, expressing interest (n = 22) or declining participation (n = 23), and 42 did not respond. All 22 clinicians who responded positively were interviewed, with 7 follow-up discussions. Results suggested that clinician-teachers encountered myriad opportunities to learn clinical medicine during spontaneous interactions with trainees. These interactions, embedded in authentic patient care, were influenced by clinician-teacher characteristics, trainee characteristics, and contextual affordances. Clinician-teachers were stimulated to learn by trainee presence and through discrete interactions with trainees. These stimuli often led to feelings of "performative pressure" to role model and teach effectively or "slowing down" in thinking, prompting clinician-teachers to engage in learning processes (e.g., reflection, collaboration, and articulation), which resulted in knowledge acquisition, reinforcement, and refinement. Conclusions: Learning through teaching is an underappreciated strategy that can help clinician-teachers improve their clinical knowledge and skills. This study uncovered some of the processes through which clinicians learn during spontaneous clinical teaching and the factors that modulate this learning.
Language	English
Publishing date	2024-02-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	96192-9
ISSN	1938-808X ; 1040-2446
ISSN (online)	1938-808X
ISSN	1040-2446
DOI	10.1097/ACM.0000000000005662
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Article ; Online: Reader Response: Normobaric Hyperoxia Combined With Endovascular Treatment for Patients With Acute Ischemic Stroke: A Randomized Controlled Clinical Trial.

Yin, Yuxin Yvonne / Sun, Qing / Yan, Bing / Wu, Di / Yu, Tao

Neurology

2023 Volume 99, Issue 23, Page(s) 1067

MeSH term(s)	Humans ; Hyperoxia/complications ; Ischemic Stroke ; Stroke/therapy ; Brain Ischemia/therapy ; Endovascular Procedures
Language	English
Publishing date	2023-01-31
Publishing country	United States
Document type	Randomized Controlled Trial ; Journal Article ; Comment
ZDB-ID	207147-2
ISSN	1526-632X ; 0028-3878
ISSN (online)	1526-632X
ISSN	0028-3878
DOI	10.1212/WNL.0000000000201587
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Article ; Online: Effect of Carbonization Behaviour of Cotton Biomass in Electrodes for Sodium‐Ion Batteries

Hridip Ranjan Sarma / Dr. Ju Sun / Yvonne Hora / Prof. Maria Forsyth / Prof. Nolene Byrne

ChemElectroChem, Vol 10, Iss 14, Pp n/a-n/a (2023)

2023

Abstract: Abstract Invited for this issue's Front Cover is the Electromaterials group at the Institute of Frontier Materials of Deakin University (Australia). The cover picture emphasizes the environmental sustainability of recycling cotton textiles to produce ... ...

Abstract	Abstract Invited for this issue's Front Cover is the Electromaterials group at the Institute of Frontier Materials of Deakin University (Australia). The cover picture emphasizes the environmental sustainability of recycling cotton textiles to produce flexible & free‐standing hard carbon anodes for sodium‐ion batteries. Read the full text of the Research Article at 10.1002/celc.202300127.
Keywords	Industrial electrochemistry ; TP250-261 ; Chemistry ; QD1-999
Language	English
Publishing date	2023-07-01T00:00:00Z
Publisher	Wiley-VCH
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Online: 3DFusion, A real-time 3D object reconstruction pipeline based on streamed instance segmented data

Sun, Xi / Jacoby, Derek / Coady, Yvonne

2023

Abstract: This paper presents a real-time segmentation and reconstruction system that utilizes RGB-D images to generate accurate and detailed individual 3D models of objects within a captured scene. Leveraging state-of-the-art instance segmentation techniques, the ...

Abstract	This paper presents a real-time segmentation and reconstruction system that utilizes RGB-D images to generate accurate and detailed individual 3D models of objects within a captured scene. Leveraging state-of-the-art instance segmentation techniques, the system performs pixel-level segmentation on RGB-D data, effectively separating foreground objects from the background. The segmented objects are then reconstructed into distinct 3D models in a high-performance computation platform. The real-time 3D modelling can be applied across various domains, including augmented/virtual reality, interior design, urban planning, road assistance, security systems, and more. To achieve real-time performance, the paper proposes a method that effectively samples consecutive frames to reduce network load while ensuring reconstruction quality. Additionally, a multi-process SLAM pipeline is adopted for parallel 3D reconstruction, enabling efficient cutting of the clustering objects into individuals. This system employs the industry-leading framework YOLO for instance segmentation. To improve YOLO's performance and accuracy, modifications were made to resolve duplicated or false detection of similar objects, ensuring the reconstructed models align with the targets. Overall, this work establishes a robust real-time system with a significant enhancement for object segmentation and reconstruction in the indoor environment. It can potentially be extended to the outdoor scenario, opening up numerous opportunities for real-world applications.
Keywords	Computer Science - Computer Vision and Pattern Recognition
Subject code	004
Publishing date	2023-11-11
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of Carbonization Behaviour of Cotton Biomass in Electrodes for Sodium‐Ion Batteries

Hridip Ranjan Sarma / Dr. Ju Sun / Yvonne Hora / Prof. Maria Forsyth / Prof. Nolene Byrne

ChemElectroChem, Vol 10, Iss 14, Pp n/a-n/a (2023)

2023

Abstract: Abstract Free‐standing hard carbon electrodes are produced from cotton biomass using a low‐cost, one‐step pathway. The free‐standing feature of the electrode eliminates the use of binders and toxic solvents. The electrochemical performance of the ... ...

Abstract	Abstract Free‐standing hard carbon electrodes are produced from cotton biomass using a low‐cost, one‐step pathway. The free‐standing feature of the electrode eliminates the use of binders and toxic solvents. The electrochemical performance of the electrodes is tested to study the correlation between Na storage and the structural properties of the hard carbon material. A remarkable specific capacity of 272 mAh g−1 at a current density of 50 mA g−1 is obtained with a high initial Coulombic efficiency of 75 % for the cotton fabric (CF) sample pyrolyzed at 1000 °C for 5 min (CF5 min). The excellent performance of the free‐standing electrode is attributed to a large interlayer spacing between the graphene layers, and a high number of oxygen‐containing functional groups on the surface. X‐ray photoelectron spectroscopy (XPS) surface characterisation shows that a thin and uniformly distributed SEI (solid electrolyte interphase) layer, mainly composed of NaF and Na2O, is formed on the CF5 min surface, whereas a thick SEI layer with a long Na+ diffusion pathway is formed on the sample pyrolyzed at 1000 °C for 10 h (CF10 h), which leads to slower reaction kinetics and poor electrochemical performance. This work proposes a scalable and economically feasible strategy to produce sodium ion anode materials with a focus on environmental sustainability and value addition to waste streams.
Keywords	carbonization ; cotton biomass ; free-standing electrode ; sodium-ion battery ; sustainable ; Industrial electrochemistry ; TP250-261 ; Chemistry ; QD1-999
Subject code	620
Language	English
Publishing date	2023-07-01T00:00:00Z
Publisher	Wiley-VCH
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Low-Cost, Real-Time Polymerase Chain Reaction System for Point-of-Care Medical Diagnosis.

Kadja, Tchamie / Liu, Chengkun / Sun, Yvonne / Chodavarapu, Vamsy P

Sensors (Basel, Switzerland)

2022 Volume 22, Issue 6

Abstract: Global health crises due to the prevailing Coronavirus Disease 2019 (COVID-19) pandemic have placed significant strain on health care facilities such as hospitals and clinics around the world. Further, foodborne and waterborne diseases are not only ... ...

Abstract	Global health crises due to the prevailing Coronavirus Disease 2019 (COVID-19) pandemic have placed significant strain on health care facilities such as hospitals and clinics around the world. Further, foodborne and waterborne diseases are not only spreading faster, but also appear to be emerging more rapidly than ever before and are able to circumvent conventional control measures. The Polymerase Chain Reaction (PCR) system is a well-known diagnostic tool for many applications in medical diagnostics, environmental monitoring, and food and water quality assessment. Here, we describe the design, development, and testing of a portable, low-cost, and real-time PCR system that can be used in emergency health crises and resource-poor situations. The described PCR system incorporates real-time reaction monitoring using fluorescence as an alternative to gel electrophoresis for reaction analysis, further decreasing the need of multiple reagents, reducing sample testing cost, and reducing sample analysis time. The bill of materials cost of the described system is approximately $340. The described PCR system utilizes a novel progressive selective proportional-integral-derivative controller that helps in reducing sample analysis time. In addition, the system employs a novel primer-based approach to quantify the initial target amplicon concentration, making it well-suited for food and water quality assessment. The developed PCR system performed DNA amplification at a level and speed comparable to larger and more expensive commercial table-top systems. The fluorescence detection sensitivity was also tested to be at the same level as commercially available multi-mode optical readers, thus making the PCR system an attractive solution for medical point-of-care and food and water quality assessment.
MeSH term(s)	COVID-19/diagnosis ; Humans ; Point-of-Care Systems ; Real-Time Polymerase Chain Reaction/methods
Language	English
Publishing date	2022-03-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s22062320
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Article ; Online: Should the Surprise Question be Used as a Prognostic Tool for People With Life-limiting Illnesses?

Chu, Christina / Engels, Yvonne / Suh, Sang-Yeon / Kim, Sun-Hyun / White, Nicola

Journal of pain and symptom management

2023 Volume 66, Issue 3, Page(s) e437–e441

Abstract: The surprise question screening tool ("Would I be surprised if this person died within the next 12 months?") was initially developed to identify possible palliative care needs. One controversial topic regarding the surprise question is whether it should ... ...

Abstract	The surprise question screening tool ("Would I be surprised if this person died within the next 12 months?") was initially developed to identify possible palliative care needs. One controversial topic regarding the surprise question is whether it should be used as a prognostic tool (predicting survival) for patients with life-limiting illnesses. In this "Controversies in Palliative Care" article, three groups of expert clinicians independently answered this question. All experts provide an overview of current literature, practical advice, and opportunities for future research. All experts reported on the inconsistency of the prognostic capabilities of the surprise question. Two of the three expert groups felt that the surprise question should not be used as a prognostic tool due to these inconsistencies. The third expert group felt that the surprise question should be used as a prognostic tool, particularly for shorter time frames. The experts all highlighted that the original rationale for the surprise question was to trigger a further conversation about future treatment and a potential shift in the focus of the care, identifying patients who many benefit from specialist palliative care or advance care planning; however, many clinicians find this discussion a difficult one to initiate. The experts agreed that the benefit of the surprise question comes from its simplicity: a one-question tool that requires no specific information about the patient's condition. More research is needed to better support the application of this tool in routine practice, particularly in noncancer populations.
MeSH term(s)	Humans ; Prognosis ; Palliative Care ; Death ; Hospice and Palliative Care Nursing
Language	English
Publishing date	2023-05-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	639142-4
ISSN	1873-6513 ; 0885-3924
ISSN (online)	1873-6513
ISSN	0885-3924
DOI	10.1016/j.jpainsymman.2023.05.003
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Article ; Online: Learning by teaching: benefits for frontline clinician-teachers.

Sun, Ning-Zi / Maniatis, Thomas / Steinert, Yvonne

Medical education

2019 Volume 53, Issue 11, Page(s) 1154–1155

MeSH term(s)	Faculty, Medical/education ; Humans ; Internal Medicine/education ; Internship and Residency/organization & administration ; Learning ; Simulation Training/organization & administration ; Teaching/organization & administration
Language	English
Publishing date	2019-10-24
Publishing country	England
Document type	Journal Article
ZDB-ID	195274-2
ISSN	1365-2923 ; 0308-0110
ISSN (online)	1365-2923
ISSN	0308-0110
DOI	10.1111/medu.13977
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Article: Environmental Nutrients Alter Bacterial and Fungal Gut Microbiomes in the Common Meadow Katydid,

Muratore, Melani / Sun, Yvonne / Prather, Chelse

Frontiers in microbiology

2020 Volume 11, Page(s) 557980

Abstract: Insect gut microbiomes consist of bacteria, fungi, and viruses that can act as mutualists to influence the health and fitness of their hosts. While much has been done to increase understanding of the effects of environmental factors that drive insect ... ...

Abstract	Insect gut microbiomes consist of bacteria, fungi, and viruses that can act as mutualists to influence the health and fitness of their hosts. While much has been done to increase understanding of the effects of environmental factors that drive insect ecology, there is less understanding of the effects of environmental factors on these gut microbial communities. For example, the effect of environmental nutrients on most insect gut microbiomes is poorly defined. To address this knowledge gap, we investigated the relationship between environmental nutrients and the gut microbial communities in a small study of katydids (
Language	English
Publishing date	2020-10-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2020.557980
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