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  1. Article: Urine Se concentration poorly predicts plasma Se concentration at sub-district scales in Zimbabwe, limiting its value as a biomarker of population Se status.

    Mutonhodza, Beaula / Dembedza, Mavis P / Joy, Edward J M / Manzeke-Kangara, Muneta G / Njovo, Handrea / Nyadzayo, Tasiana K / Lark, R Murray / Kalimbira, Alexander A / Bailey, Elizabeth H / Broadley, Martin R / Matsungo, Tonderayi M / Chopera, Prosper

    Frontiers in nutrition

    2024  Volume 11, Page(s) 1288748

    Abstract: Introduction: The current study investigated the value of urine selenium (Se) concentration as a biomarker of population Se status in rural sub-Saharan Africa.: Method: Urine and plasma Se concentrations were measured among children aged 6-59 months ( ...

    Abstract Introduction: The current study investigated the value of urine selenium (Se) concentration as a biomarker of population Se status in rural sub-Saharan Africa.
    Method: Urine and plasma Se concentrations were measured among children aged 6-59 months (
    Results: The median (Q1, Q3) urine Se concentrations were 8.4 μg/L (5.3, 13.5) and 10.5 μg/L (6.5, 15.2) in children and WRA, respectively. There was moderate evidence for a relationship between urine Se concentration and plasma Se concentration in children (
    Discussion: Urine Se concentration poorly predicted plasma Se concentration at sub-district scales in Zimbabwe, limiting its value as a biomarker of population Se status in this context. Further research is warranted at wider spatial scales to determine the value of urine Se as a biomarker when there is greater heterogeneity in Se exposure.
    Language English
    Publishing date 2024-02-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2776676-7
    ISSN 2296-861X
    ISSN 2296-861X
    DOI 10.3389/fnut.2024.1288748
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: A pilot survey of selenium status and its geospatial variation among children and women in three rural districts of Zimbabwe.

    Mutonhodza, Beaula / Chagumaira, Christopher / Dembedza, Mavis P / Joy, Edward J M / Manzeke-Kangara, Muneta G / Njovo, Handrea / Nyadzayo, Tasiana K / Lark, R Murray / Kalimbira, Alexander A / Bailey, Elizabeth H / Broadley, Martin R / Matsungo, Tonderayi M / Chopera, Prosper

    Frontiers in nutrition

    2023  Volume 10, Page(s) 1235113

    Abstract: Introduction: Selenium (Se) deficiency is increasingly recognized as a public health problem in sub-Saharan Africa.: Methods: The current cross-sectional study assessed the prevalence and geospatial patterns of Se deficiency among children aged 6-59 ... ...

    Abstract Introduction: Selenium (Se) deficiency is increasingly recognized as a public health problem in sub-Saharan Africa.
    Methods: The current cross-sectional study assessed the prevalence and geospatial patterns of Se deficiency among children aged 6-59 months (
    Results: Median, Q1, and Q3 plasma Se concentrations were 61.2, 48.7, and 73.3 μg/L for women and 40.5, 31.3, and 49.5 μg/L for children, respectively. Low plasma Se concentrations (9.41 μg/L in children and 10.20 μg/L in women) indicative of severe Se deficiency risk was observed. Overall, 94.6% of children and 69.8% of women had sub-optimal Se status defined by plasma Se concentrations of <64.8 μg/L and <70 μg/L, respectively.
    Discussion: High and widespread Se deficiency among women and children in the three districts is of public health concern and might be prevalent in other rural districts in Zimbabwe. Geostatistical analysis by conditional kriging showed a high risk of Se deficiency and that the Se status in women and children in Murewa, Shamva, and Mutasa districts was driven by short-range variations of up to ⁓12 km. Selenium status was homogenous within each district. However, there was substantial inter-district variation, indicative of marked spatial patterns if the sampling area is scaled up. A nationwide survey that explores the extent and spatial distribution of Se deficiency is warranted.
    Language English
    Publishing date 2023-07-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2776676-7
    ISSN 2296-861X
    ISSN 2296-861X
    DOI 10.3389/fnut.2023.1235113
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: An efficient plating system for rapid isolation of mutants from plant cell suspensions.

    Weber, G / Lark, K G

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

    2013  Volume 55, Issue 2, Page(s) 81–86

    Abstract: A plating system for cell suspensions of soybean, SB-1, (Glycine max L. cv. 'Mandarin') and Datura innoxia D.I. (Mill) was developed using feeder cells. The characteristics of the system are: a) the efficiency of plating (EOP) is high (0.5-0.6), b) over ... ...

    Abstract A plating system for cell suspensions of soybean, SB-1, (Glycine max L. cv. 'Mandarin') and Datura innoxia D.I. (Mill) was developed using feeder cells. The characteristics of the system are: a) the efficiency of plating (EOP) is high (0.5-0.6), b) over a range of 10-300 plated clumps the EOP is constant, c) the growth rate of plated cells resembles that of suspension cultures (generation time 24 hr.). Clumps with few or with many cells have similar plating efficiencies.Employing the plating system, a mutant resistant to 8 azaguanine (8AG) was isolated from SB-1 in 7 days and purified and tested within an additional 3 weeks. Feeder plates were used to selectively re-isolate 8 AG resistant and maltose utilizing mutants from a 1000-fold excess of wild type cells.The plating technique also can be utilized to isolate auxotrophic mutants since free amino acids are not produced by the feeder suspension. Other applications of this plating technique are discussed.
    Language English
    Publishing date 2013-12-04
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2170-2
    ISSN 1432-2242 ; 0040-5752
    ISSN (online) 1432-2242
    ISSN 0040-5752
    DOI 10.1007/BF00285195
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1692: Show issues Location:
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  4. Article: Cell-cycle parameters of soybean (Glycine max L.) cells growing in suspension culture: Suitability of the system for genetic studies.

    Chu, Y E / Lark, K G

    Planta

    2014  Volume 132, Issue 3, Page(s) 259–268

    Abstract: Suspension cultures of Glycine max (L.) Merr. were grown at 22 and 33°. The doubling times of dividing cells were 35 and 25 h, respectively. G2 was 6.2 and 6.7 h, and S was 13.8 and 6.5 h. G1 was calculated as 13 and 10 h, respectively. These values were ...

    Abstract Suspension cultures of Glycine max (L.) Merr. were grown at 22 and 33°. The doubling times of dividing cells were 35 and 25 h, respectively. G2 was 6.2 and 6.7 h, and S was 13.8 and 6.5 h. G1 was calculated as 13 and 10 h, respectively. These values were determined by labeling cells with (3)H thymidine and measuring the appearance of radioactive mitotic figures. Treatment with 5-fluorodeoxyuridine (FudR) inhibited DNA synthesis and, as a result, cells accumulated in S. Such cells were viable and, upon removal of the FudR, proceeded synchronously into mitosis. Treatment with 5-bromodeoxyuridine, following FudR synchronization, sensitized the cells to white light. Thus cells capable of synthesizing DNA could be killed. 20-30% of the cells in suspension cultures growing at 22 or 33° were not able to synthesize DNA. Nevertheless, these non-dividing (Q) cells were able to synthesize RNA and protein at a reduced rate. The proteins synthesized appear to be a particular subset of the proteins made by normal cells. The results are analyzed in relation to the use of this suspension cell culture system for isolating conditional lethal mutants of plant cells.
    Language English
    Publishing date 2014-01-14
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 208909-9
    ISSN 1432-2048 ; 0032-0935 ; 1866-2749
    ISSN (online) 1432-2048
    ISSN 0032-0935 ; 1866-2749
    DOI 10.1007/BF00399725
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    In stock of ZB MED Bonn / Germany

    Z 46/303: Show issues

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  5. Article: Sister Chromatid Segregation during Mitosis in Polyploid Wheat.

    Lark, K G

    Genetics

    2007  Volume 62, Issue 2, Page(s) 289–305

    Language English
    Publishing date 2007-01-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2167-2
    ISSN 1943-2631 ; 0016-6731
    ISSN (online) 1943-2631
    ISSN 0016-6731
    DOI 10.1093/genetics/62.2.289
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 767: Show issues Location:
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  6. Article: Accuracy, Bias, and Improvements in Mapping Crops and Cropland across the United States Using the USDA Cropland Data Layer

    Lark, Tyler J. / Schelly, Ian H. / Gibbs, Holly K.

    Remote Sensing. 2021 Mar. 04, v. 13, no. 5

    2021  

    Abstract: ... rates, which help to quantify and differentiate the efficacy of mapping aggregated land use classes (e.g ...

    Abstract The U.S. Department of Agriculture’s (USDA) Cropland Data Layer (CDL) is a 30 m resolution crop-specific land cover map produced annually to assess crops and cropland area across the conterminous United States. Despite its prominent use and value for monitoring agricultural land use/land cover (LULC), there remains substantial uncertainty surrounding the CDLs’ performance, particularly in applications measuring LULC at national scales, within aggregated classes, or changes across years. To fill this gap, we used state- and land cover class-specific accuracy statistics from the USDA from 2008 to 2016 to comprehensively characterize the performance of the CDL across space and time. We estimated nationwide area-weighted accuracies for the CDL for specific crops as well as for the aggregated classes of cropland and non-cropland. We also derived and reported new metrics of superclass accuracy and within-domain error rates, which help to quantify and differentiate the efficacy of mapping aggregated land use classes (e.g., cropland) among constituent subclasses (i.e., specific crops). We show that aggregate classes embody drastically higher accuracies, such that the CDL correctly identifies cropland from the user’s perspective 97% of the time or greater for all years since nationwide coverage began in 2008. We also quantified the mapping biases of specific crops throughout time and used these data to generate independent bias-adjusted crop area estimates, which may complement other USDA survey- and census-based crop statistics. Our overall findings demonstrate that the CDLs provide highly accurate annual measures of crops and cropland areas, and when used appropriately, are an indispensable tool for monitoring changes to agricultural landscapes.
    Keywords USDA ; cropland ; land cover ; land use ; space and time ; uncertainty
    Language English
    Dates of publication 2021-0304
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-light
    ZDB-ID 2513863-7
    ISSN 2072-4292
    ISSN 2072-4292
    DOI 10.3390/rs13050968
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Isolation of an auxotrophic cell line of soybean (Glycine max) which requires asparagine or glutamine for growth.

    Roth, E J / Lark, K G

    Plant cell reports

    2013  Volume 1, Issue 4, Page(s) 157–160

    Abstract: A suspension culture of haploid soybean cells was treated with ultra-violet light. From the mutagenized culture an auxotrophic cell line was isolated which grows on 1-B5 media supplemented with asparagine or glutamine. In the absence of these additives ... ...

    Abstract A suspension culture of haploid soybean cells was treated with ultra-violet light. From the mutagenized culture an auxotrophic cell line was isolated which grows on 1-B5 media supplemented with asparagine or glutamine. In the absence of these additives the cells cease to grow and die. Asparagine is unable to serve as a sole source of nitrogen, i.e., cannot substitute for nitrate and ammonia.
    Language English
    Publishing date 2013-11-20
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 8397-5
    ISSN 1432-203X ; 0721-7714 ; 0721-085X
    ISSN (online) 1432-203X
    ISSN 0721-7714 ; 0721-085X
    DOI 10.1007/BF00269187
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    In stock of ZB MED Bonn / Germany

    Z 85/705: Show issues

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  8. Article: Isopropyl-N(3-chlorophenyl) carbamate (CIPC) induced chromosomal loss in soybean: a new tool for plant somatic cell genetics.

    Roth, E J / Lark, K G

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

    2013  Volume 68, Issue 5, Page(s) 421–431

    Abstract: The properties and uses of tissue culture partial haploid soybean cell lines are explored. Partial haploid lines were prepared by CIPC treatment of a genetic heterozygote, and compared to this heterozygote and to homozygous cell lines corresponding to ... ...

    Abstract The properties and uses of tissue culture partial haploid soybean cell lines are explored. Partial haploid lines were prepared by CIPC treatment of a genetic heterozygote, and compared to this heterozygote and to homozygous cell lines corresponding to the parental genotypes from which the heterozygote was derived. Cell lines which lack chromosomes were characterized physiologically and with respect to a variety of isozyme markers. Often the loss of chromosomes revealed a phenotype corresponding to a recessive parental genotype. In some cases, however, new phenotypes were observed indicating a complex genotype and suggests the interaction of several genes. The implications of this for plant breeding are discussed.CIPC also was used as a tool to dissect a complex phenotype which arose as the result of mutagenesis. A mutant cell line which required asparagine for growth but also had acquired the ability to grow on allantoin as a sole source of nitrogen was treated with CIPC to remove chromosomes. The requirement for asparagine could be separated from the ability to use allantoin, demonstrating that these phenotypes were the result of separate mutations.
    Language English
    Publishing date 2013-11-20
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2170-2
    ISSN 1432-2242 ; 0040-5752
    ISSN (online) 1432-2242
    ISSN 0040-5752
    DOI 10.1007/BF00254811
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  9. Article ; Online: Reply to Falconi et al.: Economic red herrings and resistance to new modeling hinder progress in assessing ethanol's land use change.

    Lark, Tyler J / Hendricks, Nathan P / Smith, Aaron / Pates, Nicholas / Spawn-Lee, Seth A / Bougie, Matthew / Booth, Eric G / Kucharik, Christopher J / Gibbs, Holly K

    Proceedings of the National Academy of Sciences of the United States of America

    2022  Volume 119, Issue 51, Page(s) e2216091119

    MeSH term(s) Ethanol ; Motor Activity ; Seafood
    Chemical Substances Ethanol (3K9958V90M)
    Language English
    Publishing date 2022-12-13
    Publishing country United States
    Document type Letter ; Comment
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2216091119
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 1148: Show issues Location:
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  10. Article: Anemia in children aged 6-59 months was significantly associated with maternal anemia status in rural Zimbabwe.

    Mutonhodza, Beaula / Dembedza, Mavis P / Lark, Murray R / Joy, Edward J M / Manzeke-Kangara, Muneta G / Njovo, Handrea / Nyadzayo, Tasiana K / Kalimbira, Alexander A / Bailey, Elizabeth H / Broadley, Martin R / Matsungo, Tonderayi M / Chopera, Prosper

    Food science & nutrition

    2022  Volume 11, Issue 3, Page(s) 1232–1246

    Abstract: Globally, anemia is a public health problem affecting mostly women of reproductive age (WRA, ...

    Abstract Globally, anemia is a public health problem affecting mostly women of reproductive age (WRA,
    Language English
    Publishing date 2022-12-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2703010-6
    ISSN 2048-7177
    ISSN 2048-7177
    DOI 10.1002/fsn3.3157
    Database MEDical Literature Analysis and Retrieval System OnLINE

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