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Article ; Online: Integration of a side-stream microalgae process into a municipal wastewater treatment plant: A life cycle analysis.

Tua, Camilla / Ficara, Elena / Mezzanotte, Valeria / Rigamonti, Lucia

2020 Volume 279, Page(s) 111605

Abstract: This study investigates the environmental improvements associated to the integration of a microalgae unit as a side-stream process within an existing municipal wastewater treatment facility in northern Italy. Microalgae are fed on the centrate from ... ...

Abstract	This study investigates the environmental improvements associated to the integration of a microalgae unit as a side-stream process within an existing municipal wastewater treatment facility in northern Italy. Microalgae are fed on the centrate from sludge dewatering, rich in nutrients, and on the CO
MeSH term(s)	Animals ; Biofuels ; Biomass ; Italy ; Life Cycle Stages ; Microalgae ; Rivers ; Waste Water ; Water Purification
Chemical Substances	Biofuels ; Waste Water
Language	English
Publishing date	2020-11-07
Publishing country	England
Document type	Journal Article
ZDB-ID	184882-3
ISSN	1095-8630 ; 0301-4797
ISSN (online)	1095-8630
ISSN	0301-4797
DOI	10.1016/j.jenvman.2020.111605
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Article: First report of Flavescence dorée phytoplasma identification and characterization in three species of leafhoppers

Belgeri, Elena / Rizzoli, Attilio / Jermini, Mauro / Angelini, Elisa / Filippin, Luisa / Rigamonti, Ivo Ercole

Journal of plant pathology. 2022 Feb., v. 104, no. 1

2022

Abstract: Phytoplasma strains were sporadically found in specimens of Hishimonus hamatus, Japananus hyalinus and Graphocephala fennahi caught during an entomological survey conducted in Southern Switzerland on the edges and woods surrounding cultivated vineyards ... ...

Abstract	Phytoplasma strains were sporadically found in specimens of Hishimonus hamatus, Japananus hyalinus and Graphocephala fennahi caught during an entomological survey conducted in Southern Switzerland on the edges and woods surrounding cultivated vineyards in 2017. The subsequent molecular characterization of the pathogens allowed to identify them as strains strictly associated to Flavescence dorée, a serious European grapevine disease, transmitted from vine to vine by the leafhopper Scaphoideus titanus. In particular, M12 and M50 genotypes were found in H. hamatus and J. hyalinus, respectively, while G. fennahi harboured mixed phytoplasma genotypes with at least two different Flavescence dorée isolates. The potential importance of these findings in the frame of the Flavescence dorée epidemics in Europe is discussed.
Keywords	Graphocephala fennahi ; Hishimonus ; Phytoplasma ; Scaphoideus titanus ; Vitis ; insect surveys ; phytoplasmal diseases ; plant pathology ; vines ; Switzerland
Language	English
Dates of publication	2022-02
Size	p. 375-379.
Publishing place	Edizioni ETS
Document type	Article
ZDB-ID	1378427-4
ISSN	2239-7264 ; 1125-4653
ISSN (online)	2239-7264
ISSN	1125-4653
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Article: Solid-State Nonlinear Optical Properties of Mononuclear Copper(II) Complexes with Chiral Tridentate and Tetradentate Schiff Base Ligands.

Rigamonti, Luca / Forni, Alessandra / Cariati, Elena / Malavasi, Gianluca / Pasini, Alessandro

Materials (Basel, Switzerland)

2019 Volume 12, Issue 21

Abstract: Salen-type metal complexes have been actively studied for their nonlinear optical (NLO) properties, and push-pull compounds with charge asymmetry generated by electron releasing and withdrawing groups have shown promising results. As a continuation of ... ...

Abstract	Salen-type metal complexes have been actively studied for their nonlinear optical (NLO) properties, and push-pull compounds with charge asymmetry generated by electron releasing and withdrawing groups have shown promising results. As a continuation of our research in this field and aiming at solid-state features, herein we report on the synthesis of mononuclear copper(II) derivatives bearing either tridentate N
Language	English
Publishing date	2019-11-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma12213595
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Article ; Online: Role of expectations in clinical outcomes after deep brain stimulation in patients with Parkinson's disease: a systematic review.

Mameli, Francesca / Zirone, Eleonora / Girlando, Roberta / Scagliotti, Elena / Rigamonti, Giulia / Aiello, Edoardo Nicolò / Poletti, Barbara / Ferrucci, Roberta / Ticozzi, Nicola / Silani, Vincenzo / Locatelli, Marco / Barbieri, Sergio / Ruggiero, Fabiana

Journal of neurology

2023 Volume 270, Issue 11, Page(s) 5274–5287

Abstract: Deep brain stimulation (DBS) is a well-established treatment that significantly improves the motor symptoms of patients with Parkinson's disease (PD); however, patients may experience post-operative psychological distress and social maladjustments. This ... ...

Abstract	Deep brain stimulation (DBS) is a well-established treatment that significantly improves the motor symptoms of patients with Parkinson's disease (PD); however, patients may experience post-operative psychological distress and social maladjustments. This phenomenon has been shown to be related to patients' pre-operative cognitive representations, such as expectations. In this systematic review, we discuss the findings on the role of the expectations of patients with PD regarding the clinical outcomes of DBS to identify areas of intervention to improve pre-operative patient education and promote successful post-operative psychosocial adjustment. PubMed was searched for relevant articles published up to 16 January 2023. Of the 84 identified records, 10 articles focusing on the treatment expectations of patients with PD undergoing DBS were included in this review. The selected studies were conducted among cohorts of patients with different DBS targets, among which the most common was the bilateral subthalamic nucleus. Overall, the data showed that patients' expectations contribute to treatment efficacy. Experiments investigating the placebo effect itself have shown clinical improvement after the induction of positive therapeutic expectations; conversely, unrealistic treatment expectations can affect patient satisfaction after surgery, clinical outcomes, and subjective well-being. This review highlights the need for routine clinical practice to better investigate and manage patients' pre-operative expectations, as well as multidisciplinary education to improve patient satisfaction and psychosocial adjustment after DBS.
MeSH term(s)	Humans ; Parkinson Disease/drug therapy ; Deep Brain Stimulation ; Motivation ; Subthalamic Nucleus ; Treatment Outcome
Language	English
Publishing date	2023-07-30
Publishing country	Germany
Document type	Systematic Review ; Journal Article ; Review
ZDB-ID	187050-6
ISSN	1432-1459 ; 0340-5354 ; 0012-1037 ; 0939-1517 ; 1619-800X
ISSN (online)	1432-1459
ISSN	0340-5354 ; 0012-1037 ; 0939-1517 ; 1619-800X
DOI	10.1007/s00415-023-11898-6
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Article: Exposure to Plasma From Non-alcoholic Fatty Liver Disease Patients Affects Hepatocyte Viability, Generates Mitochondrial Dysfunction, and Modulates Pathways Involved in Fat Accumulation and Inflammation.

Grossini, Elena / Garhwal, Divya Praveen / Calamita, Giuseppe / Romito, Raffaele / Rigamonti, Cristina / Minisini, Rosalba / Smirne, Carlo / Surico, Daniela / Bellan, Mattia / Pirisi, Mario

Frontiers in medicine

2021 Volume 8, Page(s) 693997

Abstract: Changes of lipidic storage, oxidative stress and mitochondrial dysfunction may be involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). Although the knowledge of intracellular pathways has vastly expanded in recent years, the role ... ...

Abstract	Changes of lipidic storage, oxidative stress and mitochondrial dysfunction may be involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). Although the knowledge of intracellular pathways has vastly expanded in recent years, the role and mechanisms of circulating triggering factor(s) are debated. Thus, we tested the hypothesis that factors circulating in the blood of NAFLD patients may influence processes underlying the disease. Huh7.5 cells/primary human hepatocytes were exposed to plasma from 12 NAFLD patients and 12 healthy subjects and specific assays were performed to examine viability, H
Language	English
Publishing date	2021-07-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2775999-4
ISSN	2296-858X
ISSN	2296-858X
DOI	10.3389/fmed.2021.693997
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Article: Characterization of microRNA Levels in Synovial Fluid from Knee Osteoarthritis and Anterior Cruciate Ligament Tears.

Rizzi, Laura / Turati, Marco / Bresciani, Elena / Anghilieri, Filippo Maria / Meanti, Ramona / Molteni, Laura / Piatti, Massimiliano / Zanchi, Nicolò / Coco, Silvia / Buonanotte, Francesco / Rigamonti, Luca / Zatti, Giovanni / Locatelli, Vittorio / Omeljaniuk, Robert J / Bigoni, Marco / Torsello, Antonio

Biomedicines

2022 Volume 10, Issue 11

Abstract: This study investigated modifications of microRNA expression profiles in knee synovial fluid of patients with osteoarthritis (OA) and rupture of the anterior cruciate ligament (ACL). Twelve microRNAs (26a-5p, 27a-3p, let7a-5p, 140-5p, 146-5p, 155-5p, 16- ... ...

Abstract	This study investigated modifications of microRNA expression profiles in knee synovial fluid of patients with osteoarthritis (OA) and rupture of the anterior cruciate ligament (ACL). Twelve microRNAs (26a-5p, 27a-3p, let7a-5p, 140-5p, 146-5p, 155-5p, 16-5p,186-5p, 199a-3p, 210-3p, 205-5p, and 30b-5p) were measured by real-time quantitative polymerase chain reaction (RT-qPCR) in synovial fluids obtained from 30 patients with ACL tear and 18 patients with knee OA. These 12 miRNAs were chosen on the basis of their involvement in pathological processes of bone and cartilage. Our results show that miR-26a-5p, miR-186-5p, and miR-30b-5p were expressed in the majority of OA and ACL tear samples, whereas miR-199a-3p, miR-210-3p, and miR-205-5p were detectable only in a few samples. Interestingly, miR-140-5p was expressed in only one sample of thirty in the ACL tear group. miR-140-5p has been proposed to modulate two genes (BGN and COL5A1100) that are involved in ligamentous homeostasis; their altered expression could be linked with ACL rupture susceptibility. The expression of miR-30b-5p was higher in OA and chronic ACL groups compared to acute ACL samples. We provide evidence that specific miRNAs could be detected not only in synovial fluid of patients with OA, but also in post-traumatic ACL tears.
Language	English
Publishing date	2022-11-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720867-9
ISSN	2227-9059
ISSN	2227-9059
DOI	10.3390/biomedicines10112909
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Article ; Online: Doxycycline rescues recognition memory and circadian motor rhythmicity but does not prevent terminal disease in fatal familial insomnia mice.

Lavigna, Giada / Masone, Antonio / Bouybayoune, Ihssane / Bertani, Ilaria / Lucchetti, Jacopo / Gobbi, Marco / Porcu, Luca / Zordan, Stefano / Rigamonti, Mara / Imeri, Luca / Restelli, Elena / Chiesa, Roberto

Neurobiology of disease

2021 Volume 158, Page(s) 105455

Abstract: Fatal familial insomnia (FFI) is a dominantly inherited prion disease linked to the D178N mutation in the gene encoding the prion protein (PrP). Symptoms, including insomnia, memory loss and motor abnormalities, appear around 50 years of age, leading to ... ...

Abstract	Fatal familial insomnia (FFI) is a dominantly inherited prion disease linked to the D178N mutation in the gene encoding the prion protein (PrP). Symptoms, including insomnia, memory loss and motor abnormalities, appear around 50 years of age, leading to death within two years. No treatment is available. A ten-year clinical trial of doxycycline (doxy) is under way in healthy individuals at risk of FFI to test whether presymptomatic doxy prevents or delays the onset of disease. To assess the drug's effect in a tractable disease model, we used Tg(FFI-26) mice, which accumulate aggregated and protease-resistant PrP in their brains and develop a fatal neurological illness highly reminiscent of FFI. Mice were treated daily with 10 mg/kg doxy starting from a presymptomatic stage for twenty weeks. Doxy rescued memory deficits and restored circadian motor rhythmicity in Tg(FFI-26) mice. However, it did not prevent the onset and progression of motor dysfunction, clinical signs and progression to terminal disease. Doxy did not change the amount of aggregated and protease-resistant PrP, but reduced microglial activation in the hippocampus. Presymptomatic doxy treatment rescues cognitive impairment and the motor correlates of sleep dysfunction in Tg(FFI-26) mice but does not prevent fatal disease.
MeSH term(s)	Animals ; Brain/pathology ; Circadian Rhythm/drug effects ; Disease Progression ; Doxycycline/pharmacology ; Doxycycline/therapeutic use ; Insomnia, Fatal Familial/drug therapy ; Insomnia, Fatal Familial/genetics ; Insomnia, Fatal Familial/pathology ; Memory/drug effects ; Mice ; Mice, Inbred C57BL ; Motor Activity/drug effects ; Psychomotor Performance/drug effects ; Recognition, Psychology/drug effects
Chemical Substances	Doxycycline (N12000U13O)
Language	English
Publishing date	2021-08-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1211786-9
ISSN	1095-953X ; 0969-9961
ISSN (online)	1095-953X
ISSN	0969-9961
DOI	10.1016/j.nbd.2021.105455
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Article: Intranasal Low-Dose Naltrexone Against Opioid Side Effects: A Preclinical Study.

Micheli, Laura / Di Cesare Mannelli, Lorenzo / Lucarini, Elena / Parisio, Carmen / Toti, Alessandra / Fiorentino, Bruno / Rigamonti, Maria Adele / Calosi, Laura / Ghelardini, Carla

Frontiers in pharmacology

2020 Volume 11, Page(s) 576624

Abstract: Opioids are broad spectrum analgesics that are an integral part of the therapeutic armamentarium to combat pain in the clinical practice. Unfortunately, together with analgesia, a number of adverse effects can occur such as nausea, vomiting, constipation, ...

Abstract	Opioids are broad spectrum analgesics that are an integral part of the therapeutic armamentarium to combat pain in the clinical practice. Unfortunately, together with analgesia, a number of adverse effects can occur such as nausea, vomiting, constipation, gastrointestinal alterations and cognitive impairments. Naltrexone is a competitive antagonist of opioid receptors commonly used to treat opioid addiction; its oral use against agonists side effects is limited by the decrease of opioids-therapeutic efficacy and own adverse effects. The intranasal delivery of naltrexone could offer a quick and effective achievement of CNS based on extracellular mechanisms including perineural and perivascular transport. The aim of the study was to test the efficacy of intranasal low-dose naltrexone in reducing intraperitoneal morphine and oxycodone side effects in rodents. In mice, 1 μg naltrexone intranasally administered 30 min before opioids reduced cognitive impairments and motor alteration induced by 10 mg kg
Language	English
Publishing date	2020-09-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2020.576624
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Article ; Online: Dynamic molecular exchange and conformational transitions of alpha-synuclein at the nano-bio interface.

Tira, Roberto / De Cecco, Elena / Rigamonti, Valentina / Santambrogio, Carlo / Barracchia, Carlo Giorgio / Munari, Francesca / Romeo, Alessandro / Legname, Giuseppe / Prosperi, Davide / Grandori, Rita / Assfalg, Michael

International journal of biological macromolecules

2020 Volume 154, Page(s) 206–216

Abstract: The notion that nanoscale surfaces influence protein conformational transitions stimulates the investigation of fibrillogenic polypeptides adsorbing to nanomaterials. Alpha-synuclein (αS) is a prototypical amyloidogenic protein whose aggregation is ... ...

Abstract	The notion that nanoscale surfaces influence protein conformational transitions stimulates the investigation of fibrillogenic polypeptides adsorbing to nanomaterials. Alpha-synuclein (αS) is a prototypical amyloidogenic protein whose aggregation is associated with severe neurodegenerative disorders. We explored the interaction of αS with silica nanoparticles (SNPs) in diverse solution conditions, ranging from protein-free to protein-rich media. We found that the SNP-binding region of αS, determined by site-resolved NMR spectroscopy, was similar in simple buffer and blood serum. Competition binding experiments with isotopic homologues and different proteins showed that cosolutes elicited molecular exchange in a protein-specific manner. The interaction of an oxidized, fibrillation-resistant protein form with SNPs was similar to that of unmodified αS. SNPs, however, did not stimulate fibrillation of the oxidized protein, which remained fibrillation incompetent. CD experiments revealed SNP-induced perturbations of the structural properties of oxidized and non-oxidized αS. Thus, while αS binding to SNPs is essentially orthogonal to fibril formation, the interaction perturbs the distribution of conformational states populated by the protein in the colloidal suspension. This study sheds light on the dynamic nature of αS interactions with NPs, an aspect that crucially impacts on our ability to control aggregation of αS.
MeSH term(s)	Humans ; Nanoparticles/chemistry ; Protein Aggregation, Pathological ; Protein Binding ; Protein Conformation ; Protein Folding ; Recombinant Proteins/chemistry ; Silicon Dioxide/chemistry ; alpha-Synuclein/chemistry
Chemical Substances	Recombinant Proteins ; SNCA protein, human ; alpha-Synuclein ; Silicon Dioxide (7631-86-9)
Language	English
Publishing date	2020-03-14
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2020.03.118
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Article ; Online: Prognostic value of global left atrial peak strain in patients with acute ischemic stroke and no evidence of atrial fibrillation.

Sonaglioni, Andrea / Vincenti, Antonio / Baravelli, Massimo / Rigamonti, Elisabetta / Tagliabue, Elena / Bassi, Pietro / Nicolosi, Gian Luigi / Anzà, Claudio / Lombardo, Michele

The international journal of cardiovascular imaging

2018 Volume 35, Issue 4, Page(s) 603–613

Abstract: Prognostic stratification of acute ischemic stroke (AIS) patients without atrial fibrillation (AF) remains a challenge. Two-dimensional speckle tracking echocardiography (2D-STE) has recently been introduced for dynamic evaluation of left atrial function. ...

Abstract	Prognostic stratification of acute ischemic stroke (AIS) patients without atrial fibrillation (AF) remains a challenge. Two-dimensional speckle tracking echocardiography (2D-STE) has recently been introduced for dynamic evaluation of left atrial function. However only few data are actually available regarding the application of 2D-STE in AIS patients. The aim of our study was to assess the prognostic role of global left atrial peak strain (GLAPS), measured by 2D-STE, in AIS patients without AF history. Eighty-five AIS patients (mean age 74.1 ± 12.1 years, 49 males) with normal sinus rhythm on ECG and without AF history were enrolled in the prospective study. All patients underwent a complete echocardiographic study with 2D-STE. At 1 year follow-up, we evaluated the occurrence of a composite endpoint of all-cause mortality plus cardiovascular re-hospitalizations. GLAPS was markedly reduced in AIS patients (15.71 ± 4.70%), without any statistically significant difference between the stroke subtypes. At 1-year follow-up, 14 deaths and 17 hospital readmissions were detected in AIS subjects. On a multivariate Cox model, variables independently associated with the occurrence of the composite endpoint were the "Rankin in" Scale (HR 1.69, p = 0.001), GFR (HR 0.98, p = 0.03) and the GLAPS value (HR 0.78, p < 0.0001). A GLAPS value ≤ 15.5% predicted the composite endpoint with sensitivity of 100% and specificity of 80%. A GLAPS value ≤ 15.5% reflects a more advanced atrial cardiomyopathy and might provide a reliable and useful prognostic risk stratification of AIS patients without AF history.
MeSH term(s)	Aged ; Aged, 80 and over ; Atrial Function, Left ; Brain Ischemia/diagnostic imaging ; Brain Ischemia/pathology ; Brain Ischemia/physiopathology ; Brain Ischemia/therapy ; Echocardiography, Doppler ; Female ; Heart Atria/diagnostic imaging ; Heart Atria/physiopathology ; Humans ; Male ; Middle Aged ; Patient Readmission ; Predictive Value of Tests ; Prognosis ; Prospective Studies ; Risk Factors ; Stroke/diagnostic imaging ; Stroke/mortality ; Stroke/physiopathology ; Stroke/therapy
Language	English
Publishing date	2018-10-30
Publishing country	United States
Document type	Journal Article ; Observational Study
ZDB-ID	2055311-0
ISSN	1875-8312 ; 1573-0743 ; 1569-5794 ; 0167-9899
ISSN (online)	1875-8312 ; 1573-0743
ISSN	1569-5794 ; 0167-9899
DOI	10.1007/s10554-018-1485-z
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