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  1. Article ; Online: SERCA2a microdomain cAMP changes in heart failure with preserved ejection fraction.

    Gotthardt, Michael / Lehnart, Stephan E

    Cardiovascular research

    2024  Volume 120, Issue 3, Page(s) 220–222

    MeSH term(s) Humans ; Stroke Volume ; Diabetes Mellitus, Type 2 ; Ventricular Function, Left ; Obesity ; Heart Failure
    Language English
    Publishing date 2024-01-24
    Publishing country England
    Document type Editorial ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 80340-6
    ISSN 1755-3245 ; 0008-6363
    ISSN (online) 1755-3245
    ISSN 0008-6363
    DOI 10.1093/cvr/cvae030
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    Zs.A 565: Show issues Location:
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  2. Article: Editorial: Cardiac optogenetics: Using light to observe and excite the heart.

    Bruegmann, Tobias / Smith, Godfrey L / Lehnart, Stephan E

    Frontiers in physiology

    2022  Volume 13, Page(s) 1031062

    Language English
    Publishing date 2022-10-11
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2564217-0
    ISSN 1664-042X
    ISSN 1664-042X
    DOI 10.3389/fphys.2022.1031062
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  3. Book ; Online ; Thesis: Dreifarbige STED-Nanoskopie von Calcium-Freisetzungseinheiten in humanem linksventrikulären Gewebe verschiedener Subtypen hochgradiger Aortenklappenstenose

    Schönberger, Hanne-Lea [Verfasser] / Lehnart, Stephan E. [Akademischer Betreuer] / Lehnart, Stephan E. [Gutachter] / Zeisberg, Elisabeth [Gutachter]

    2023  

    Author's details Hanne-Lea Schönberger ; Gutachter: Stephan E. Lehnart, Elisabeth Zeisberg ; Betreuer: Stephan E. Lehnart
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Niedersächsische Staats- und Universitätsbibliothek Göttingen
    Publishing place Göttingen
    Document type Book ; Online ; Thesis
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  4. Article ; Online: Noninvasive analysis of contractility during identical maturations revealed two phenotypes in ventricular but not in atrial iPSC-CM.

    Rapöhn, Marcel / Cyganek, Lukas / Voigt, Niels / Hasenfuß, Gerd / Lehnart, Stephan E / Wegener, Jörg W

    American journal of physiology. Heart and circulatory physiology

    2024  Volume 326, Issue 3, Page(s) H599–H611

    Abstract: Patient-derived induced pluripotent stem cells (iPSCs) can be differentiated into atrial and ventricular cardiomyocytes to allow for personalized drug screening. A hallmark of differentiation is the manifestation of spontaneous beating in a two- ... ...

    Abstract Patient-derived induced pluripotent stem cells (iPSCs) can be differentiated into atrial and ventricular cardiomyocytes to allow for personalized drug screening. A hallmark of differentiation is the manifestation of spontaneous beating in a two-dimensional (2-D) cell culture. However, an outstanding observation is the high variability in this maturation process. We valued that contractile parameters change during differentiation serving as an indicator of maturation. Consequently, we recorded noninvasively spontaneous motion activity during the differentiation of male iPSC toward iPSC cardiomyocytes (iPSC-CMs) to further analyze similar maturated iPSC-CMs. Surprisingly, our results show that identical differentiations into ventricular iPSC-CMs are variable with respect to contractile parameters resulting in two distinct subpopulations of ventricular-like cells. In contrast, differentiation into atrial iPSC-CMs resulted in only one phenotype. We propose that the noninvasive and cost-effective recording of contractile activity during maturation using a smartphone device may help to reduce the variability in results frequently reported in studies on ventricular iPSC-CMs.
    MeSH term(s) Humans ; Male ; Induced Pluripotent Stem Cells ; Myocytes, Cardiac ; Cell Differentiation ; Phenotype ; Heart Ventricles
    Language English
    Publishing date 2024-01-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 603838-4
    ISSN 1522-1539 ; 0363-6135
    ISSN (online) 1522-1539
    ISSN 0363-6135
    DOI 10.1152/ajpheart.00527.2023
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  5. Article ; Online: The role of junctophilin proteins in cellular function.

    Lehnart, Stephan E / Wehrens, Xander H T

    Physiological reviews

    2022  Volume 102, Issue 3, Page(s) 1211–1261

    Abstract: Junctophilins (JPHs) comprise a family of structural proteins that connect the plasma membrane to intracellular organelles such as the endo/sarcoplasmic reticulum (ER/SR). Tethering of these membrane structures results in the formation of highly ... ...

    Abstract Junctophilins (JPHs) comprise a family of structural proteins that connect the plasma membrane to intracellular organelles such as the endo/sarcoplasmic reticulum (ER/SR). Tethering of these membrane structures results in the formation of highly organized subcellular junctions that play important signaling roles in all excitable cell types. There are four JPH isoforms, expressed primarily in muscle and neuronal cell types. Each JPH protein consists of six membrane occupation and recognition nexus (MORN) motifs, a joining region connecting these to another set of two MORN motifs, a putative alpha-helical region, a divergent region exhibiting low homology between JPH isoforms, and a carboxy-terminal transmembrane region anchoring into the ER/SR membrane. JPH isoforms play essential roles in developing and maintaining subcellular membrane junctions. Conversely, inherited mutations in JPH2 cause hypertrophic or dilated cardiomyopathy, while trinucleotide expansions in the JPH3 gene cause Huntington Disease-Like 2. Loss of JPH1 protein levels can cause skeletal myopathy, while loss of cardiac JPH2 levels causes heart failure and atrial fibrillation, among other disease. This review will provide a comprehensive overview of the JPH gene family, phylogeny, and evolutionary analysis of JPH genes and other MORN domain proteins. JPH biogenesis, membrane tethering, and binding partners will be discussed, as well as functional roles of JPH isoforms in excitable cells. Finally, potential roles of JPH isoform deficits in human disease pathogenesis will be reviewed.
    MeSH term(s) Cell Membrane/metabolism ; Cell Physiological Phenomena ; Humans ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Muscular Diseases
    Chemical Substances Membrane Proteins ; junctophilin
    Language English
    Publishing date 2022-01-10
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 209902-0
    ISSN 1522-1210 ; 0031-9333
    ISSN (online) 1522-1210
    ISSN 0031-9333
    DOI 10.1152/physrev.00024.2021
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  6. Book ; Thesis: Der Einfluß von human, porcine Endothelin-1 auf die Funktion des menschlichen Herzmuskels und die Beteiligung des Natrium-Protonen-Antiports an den inotropen Wirkungen

    Lehnart, Stephan Elmar

    1998  

    Author's details von Stephan Elmar Lehnart
    Language German
    Size VI, 96 Bl. : graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Freiburg (Breisgau), Univ., Diss., 1998
    HBZ-ID HT009969080
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    99 E 578 order for loan Magazin

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  7. Book ; Online ; Thesis: Redox regulation of ryanodine receptor/Ca2+ release channels by NOX enzymes in hypertrophy and heart failure

    Uhlenkamp, Dennis [Verfasser] / Lehnart, Stephan E. [Akademischer Betreuer] / Lehnart, Stephan E. [Gutachter] / Katschinski, Dörthe [Gutachter]

    2022  

    Author's details Dennis Uhlenkamp ; Gutachter: Stephan E. Lehnart, Dörthe Katschinski ; Betreuer: Stephan E. Lehnart
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language English
    Publisher Niedersächsische Staats- und Universitätsbibliothek Göttingen
    Publishing place Göttingen
    Document type Book ; Online ; Thesis
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  8. Article ; Online: Ryanodine receptor dysfunction and the resolution revolution: how Nobel Prize-winning techniques transform cardiovascular research.

    Voigt, Niels / Lehnart, Stephan E

    Cardiovascular research

    2019  Volume 114, Issue 14, Page(s) e106–e109

    MeSH term(s) Calcium Signaling ; Cardiovascular System ; Homeostasis ; Nobel Prize ; Ryanodine Receptor Calcium Release Channel
    Chemical Substances Ryanodine Receptor Calcium Release Channel
    Language English
    Publishing date 2019-01-04
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 80340-6
    ISSN 1755-3245 ; 0008-6363
    ISSN (online) 1755-3245
    ISSN 0008-6363
    DOI 10.1093/cvr/cvy235
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  9. Article ; Online: Cardiac multiscale bioimaging: from nano- through micro- to mesoscales.

    Tolstik, Elen / Lehnart, Stephan E / Soeller, Christian / Lorenz, Kristina / Sacconi, Leonardo

    Trends in biotechnology

    2023  Volume 42, Issue 2, Page(s) 212–227

    Abstract: Cardiac multiscale bioimaging is an emerging field that aims to provide a comprehensive understanding of the heart and its functions at various levels, from the molecular to the entire organ. It combines both physiologically and clinically relevant ... ...

    Abstract Cardiac multiscale bioimaging is an emerging field that aims to provide a comprehensive understanding of the heart and its functions at various levels, from the molecular to the entire organ. It combines both physiologically and clinically relevant dimensions: from nano- and micrometer resolution imaging based on vibrational spectroscopy and high-resolution microscopy to assess molecular processes in cardiac cells and myocardial tissue, to mesoscale structural investigations to improve the understanding of cardiac (patho)physiology. Tailored super-resolution deep microscopy with advanced proteomic methods and hands-on experience are thus strategically combined to improve the quality of cardiovascular research and support future medical decision-making by gaining additional biomolecular information for translational and diagnostic applications.
    MeSH term(s) Proteomics ; Heart/diagnostic imaging
    Language English
    Publishing date 2023-10-06
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 47474-5
    ISSN 1879-3096 ; 0167-7799
    ISSN (online) 1879-3096
    ISSN 0167-7799
    DOI 10.1016/j.tibtech.2023.08.007
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    Zs.A 2750: Show issues Location:
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  10. Article ; Online: 3D Super-Resolution Nuclear Q-FISH Imaging Reveals Cell-Cycle-Related Telomere Changes.

    Pochechueva, Tatiana V / Schwenzer, Niko / Kohl, Tobias / Brandenburg, Sören / Kaltenecker, Gesa / Wollnik, Bernd / Lehnart, Stephan E

    International journal of molecular sciences

    2024  Volume 25, Issue 6

    Abstract: We present novel workflows for Q-FISH nanoscopy with the potential for prognostic applications and resolving novel chromatin compaction changes. DNA-fluorescence in situ hybridization (DNA-FISH) is a routine application to visualize telomeres, repetitive ...

    Abstract We present novel workflows for Q-FISH nanoscopy with the potential for prognostic applications and resolving novel chromatin compaction changes. DNA-fluorescence in situ hybridization (DNA-FISH) is a routine application to visualize telomeres, repetitive terminal DNA sequences, in cells and tissues. Telomere attrition is associated with inherited and acquired diseases, including cancer and cardiomyopathies, and is frequently analyzed by quantitative (Q)-FISH microscopy. Recently, nanoscopic imaging techniques have resolved individual telomere dimensions and their compaction as a prognostic marker, in part leading to conflicting conclusions still unresolved to date. Here, we developed a comprehensive Q-FISH nanoscopy workflow to assess telomeres with PNA telomere probes and 3D-Stimulated Emission Depletion (STED) microscopy combined with Dynamic Intensity Minimum (DyMIN) scanning. We achieved single-telomere resolution at high, unprecedented telomere coverage. Importantly, our approach revealed a decrease in telomere signal density during mitotic cell division compared to interphase. Innovatively expanding FISH-STED applications, we conducted double FISH targeting of both telomere- and chromosome-specific sub-telomeric regions and accomplished FISH-STED in human cardiac biopsies. In summary, this work further advanced Q-FISH nanoscopy, detected a new aspect of telomere compaction related to the cell cycle, and laid the groundwork for future applications in complex cell types such as post-mitotic neurons and muscle cells.
    MeSH term(s) Humans ; In Situ Hybridization, Fluorescence/methods ; Telomere/genetics ; Cell Cycle/genetics ; Cell Division ; DNA
    Chemical Substances DNA (9007-49-2)
    Language English
    Publishing date 2024-03-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms25063183
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