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  1. Article ; Online: An unusual cause of proteinuria: lessons for clinical nephrologists.

    Aksenova, Marina / Zaikova, Natalia / Podgorny, Andrey / Kostereva, Kristina

    Journal of nephrology

    2022  Volume 35, Issue 9, Page(s) 2403–2405

    MeSH term(s) Humans ; Nephrologists ; Proteinuria/diagnosis ; Proteinuria/etiology ; Kidney Diseases/complications ; Urine
    Language English
    Publishing date 2022-09-23
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 1093991-x
    ISSN 1724-6059 ; 1120-3625 ; 1121-8428
    ISSN (online) 1724-6059
    ISSN 1120-3625 ; 1121-8428
    DOI 10.1007/s40620-022-01457-z
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    Zs.A 3369: Show issues Location:
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  2. Article: Role of Src family kinases and N-Myc in spermatogonial stem cell proliferation.

    Braydich-Stolle, Laura / Kostereva, Natalia / Dym, Martin / Hofmann, Marie-Claude

    Developmental biology

    2007  Volume 304, Issue 1, Page(s) 34–45

    Abstract: ... their action through a PI3K/Akt-dependent pathway to up-regulate N-myc expression. Thus, to proliferate ...

    Abstract Spermatogonial stem cells are required for the initiation of spermatogenesis and the continuous production of sperm. In addition, they can acquire pluripotency and differentiate into derivatives of the three embryonic germ layers when cultured in the appropriate conditions. Therefore, understanding the signaling pathways that lead to self-renewal or differentiation of these cells is of paramount importance for the treatment of infertility, the development of male contraceptives, the treatment of testicular cancers, and ultimately for tissue regeneration. In this report, we studied some of the signaling pathways triggered by glial cell line-derived neurotrophic factor (GDNF), a component of the spermatogonial stem cell niche produced by the somatic Sertoli cells. As model systems, we used primary cultures of mouse spermatogonial stem cells, a mouse spermatogonial stem cell line and freshly isolated testicular tubules. We report here that GDNF promotes spermatogonial stem cell proliferation through activation of members of the Src kinase family, and that these kinases exert their action through a PI3K/Akt-dependent pathway to up-regulate N-myc expression. Thus, to proliferate, spermatogonial stem cells activate mechanisms that are similar to the processes observed in brain stem cells and lung progenitors.
    MeSH term(s) Animals ; Blotting, Western ; Cell Line ; Cell Proliferation ; Gene Expression Regulation, Developmental/physiology ; Glial Cell Line-Derived Neurotrophic Factor/metabolism ; Male ; Mice ; Mice, Inbred BALB C ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/physiology ; Proto-Oncogene Proteins c-myc/metabolism ; RNA Interference ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction/physiology ; Spermatogonia/cytology ; src-Family Kinases/metabolism
    Chemical Substances Glial Cell Line-Derived Neurotrophic Factor ; Proto-Oncogene Proteins c-myc ; src-Family Kinases (EC 2.7.10.2)
    Language English
    Publishing date 2007-04-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1114-9
    ISSN 1095-564X ; 0012-1606
    ISSN (online) 1095-564X
    ISSN 0012-1606
    DOI 10.1016/j.ydbio.2006.12.013
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  3. Article: Effect of Systemic Adipose-derived Stem Cell Therapy on Functional Nerve Regeneration in a Rodent Model.

    Schweizer, Riccardo / Schnider, Jonas T / Fanzio, Paolo M / Tsuji, Wakako / Kostereva, Nataliya / Solari, Mario G / Plock, Jan A / Gorantla, Vijay S

    Plastic and reconstructive surgery. Global open

    2020  Volume 8, Issue 7, Page(s) e2953

    Abstract: ... model.: Methods: Lewis rats were divided into 3 groups: 10 mm sciatic nerve resection ("CUT" group; n ... 10), transection and repair ("REP" group; n = 10), transection and repair plus single-dose ASCs ("ASC ... group; n = 12). Allogeneic (Brown Norway rat) ASCs (1 × 10: Results: The ASC group showed accelerated ...

    Abstract Regardless of etiology, peripheral nerve injuries (PNI) result in disruption/loss of neuromuscular junctions, target muscle denervation, and poor sensorimotor outcomes with associated pain and disability. Adipose-derived stem cells (ASCs) have shown promise in neuroregeneration. However, there is a paucity of objective assessments reflective of functional neuroregeneration in experimental PNI. Here, we use a multimodal, static, and dynamic approach to evaluate functional outcomes after ASC therapy in a rodent PNI model.
    Methods: Lewis rats were divided into 3 groups: 10 mm sciatic nerve resection ("CUT" group; n = 10), transection and repair ("REP" group; n = 10), transection and repair plus single-dose ASCs ("ASC" group; n = 12). Allogeneic (Brown Norway rat) ASCs (1 × 10
    Results: The ASC group showed accelerated functional recovery on the swim test at 2 weeks postoperatively, with continued improvement over 4 weeks, culminating in superior overall outcomes at 6 weeks compared with the REP group. The CUT group showed no significant improvement from baseline. Nerve histomorphometry correlated well with the swim test results in the ASC group. Gastrocnemius muscle weights showed no difference between the REP and the ASC groups.
    Conclusion: Our study confirms that early, single dose, systemic administration of ASC after PNI accelerates and enhances overall motor recovery on static and dynamic functional tests as evidenced by improvements in voluntary as well as involuntary motions.
    Language English
    Publishing date 2020-07-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2851682-5
    ISSN 2169-7574 ; 2169-7574
    ISSN (online) 2169-7574
    ISSN 2169-7574
    DOI 10.1097/GOX.0000000000002953
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  4. Article ; Online: Regulation of the spermatogonial stem cell niche.

    Kostereva, N / Hofmann, M-C

    Reproduction in domestic animals = Zuchthygiene

    2008  Volume 43 Suppl 2, Page(s) 386–392

    Abstract: Spermatogonial stem cells (SSCs) reside within specialized microenvironments called 'niches', which are essential for their maintenance and self-renewal. In the mammalian testis, the main components of the niche include the Sertoli cell, the growth ... ...

    Abstract Spermatogonial stem cells (SSCs) reside within specialized microenvironments called 'niches', which are essential for their maintenance and self-renewal. In the mammalian testis, the main components of the niche include the Sertoli cell, the growth factors that this nursing cell produces, the basement membrane, and stimuli from the vascular network between the seminiferous tubules. This review focuses on signalling pathways maintaining SSCs self-renewal and differentiation and describes potential mechanisms of regulation of the spermatogonial stem cell niche.
    MeSH term(s) Animals ; Glial Cell Line-Derived Neurotrophic Factor/metabolism ; Male ; Mammals/metabolism ; Signal Transduction ; Spermatogonia/cytology ; Spermatogonia/metabolism ; Stem Cells/cytology ; Transcription Factors/metabolism
    Chemical Substances Glial Cell Line-Derived Neurotrophic Factor ; Transcription Factors
    Language English
    Publishing date 2008-07-11
    Publishing country Germany
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 1015187-4
    ISSN 1439-0531 ; 0936-6768
    ISSN (online) 1439-0531
    ISSN 0936-6768
    DOI 10.1111/j.1439-0531.2008.01189.x
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    Z 2117: Show issues

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  5. Article: Regulation of the Spermatogonial Stem Cell Niche

    Kostereva, N / Hofmann, M-C

    Reproduction in domestic animals. 2008 July, v. 43, no. s2

    2008  

    Abstract: Spermatogonial stem cells (SSCs) reside within specialized microenvironments called 'niches', which are essential for their maintenance and self-renewal. In the mammalian testis, the main components of the niche include the Sertoli cell, the growth ... ...

    Abstract Spermatogonial stem cells (SSCs) reside within specialized microenvironments called 'niches', which are essential for their maintenance and self-renewal. In the mammalian testis, the main components of the niche include the Sertoli cell, the growth factors that this nursing cell produces, the basement membrane, and stimuli from the vascular network between the seminiferous tubules. This review focuses on signalling pathways maintaining SSCs self-renewal and differentiation and describes potential mechanisms of regulation of the spermatogonial stem cell niche.
    Keywords basement membrane ; growth factors ; seminiferous tubules ; stem cells
    Language English
    Dates of publication 2008-07
    Size p. 386-392.
    Publisher Blackwell Publishing Ltd
    Publishing place Oxford, UK
    Document type Article
    ZDB-ID 1015187-4
    ISSN 1439-0531 ; 0936-6768
    ISSN (online) 1439-0531
    ISSN 0936-6768
    DOI 10.1111/j.1439-0531.2008.01189.x
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  6. Article ; Online: Adipose stem cells enhance excisional wound healing in a porcine model.

    James, Isaac / Bourne, Debra / Silva, Mayara / Havis, Emmanuelle / Albright, Kassandra / Zhang, Liyong / Kostereva, Nataliya / Wang, Sheri / DiBernardo, Gabriella / Guest, Rachel / Lei, Jenny / Almadori, Aurora / Satish, Latha / Marra, Kacey / Rubin, J Peter

    The Journal of surgical research

    2018  Volume 229, Page(s) 243–253

    Abstract: Background: Adipose-derived stem cells (ASCs) are capable of secreting regenerative growth factors and replacing multiple tissue types. Although current literature suggests that ASCs accelerate wound healing and reduce scarring, the dose-response ... ...

    Abstract Background: Adipose-derived stem cells (ASCs) are capable of secreting regenerative growth factors and replacing multiple tissue types. Although current literature suggests that ASCs accelerate wound healing and reduce scarring, the dose-response relationship has not been adequately investigated in large animals. We sought to establish a porcine model to optimize dose and delivery.
    Methods: Four-centimeter circular, full thickness excisional wounds were created on the backs of Yorkshire pigs. Fluorescently labeled allogeneic porcine ASCs were injected into the superficial wound bed and around the wound perimeter at high (3.0 × 10
    Results: Labeled ASCs integrated into treated wounds by 1 wk in a dose-dependent fashion. Epithelial coverage was achieved by 14 d in all wounds. Wounds receiving high-dose ASCs exhibited thicker granulating neodermis at 7 d and greater wound contraction at 14 d. real-time quantitative reverse transcriptase polymerase chain reaction revealed improved collagen 1:collagen 3 (Col1:Col3) ratio in the medium-dose group and enhanced α-smooth muscle actin in the high-dose group at 14 d. Western blot demonstrated increased cluster of differentiation 31 protein at 2 wk in wounds receiving >10
    Conclusions: Doses up to 3.0 × 10
    MeSH term(s) Adipose Tissue/cytology ; Animals ; Cell Differentiation ; Cicatrix/etiology ; Cicatrix/prevention & control ; Disease Models, Animal ; Female ; Humans ; Intercellular Signaling Peptides and Proteins/metabolism ; Neovascularization, Physiologic/physiology ; Regeneration/physiology ; Skin/blood supply ; Skin/injuries ; Stem Cell Transplantation/methods ; Stem Cells/physiology ; Sus scrofa ; Wound Healing/physiology ; Wounds, Penetrating/complications ; Wounds, Penetrating/therapy
    Chemical Substances Intercellular Signaling Peptides and Proteins
    Language English
    Publishing date 2018-05-03
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 80170-7
    ISSN 1095-8673 ; 0022-4804
    ISSN (online) 1095-8673
    ISSN 0022-4804
    DOI 10.1016/j.jss.2018.03.068
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    Zs.A 178: Show issues Location:
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  7. Article: [Cultivation and transplantation of boar type A spermatogonia].

    Savchenkova, I P / Korzhikova, S V / Kostereva, N V / Ernst, L K

    Ontogenez

    2006  Volume 37, Issue 4, Page(s) 292–300

    Abstract: A cell population enriched with type A spermatogonia has been isolated from the boar testes. Cell types occurring during isolation were morphologically characterized, factors maintaining the cultured spermatogonia in the undifferentiated state were ... ...

    Abstract A cell population enriched with type A spermatogonia has been isolated from the boar testes. Cell types occurring during isolation were morphologically characterized, factors maintaining the cultured spermatogonia in the undifferentiated state were studied, and these cells were transferred to sterile recipients preliminarily treated with busulfan. The cells of spermatogenic epithelium cultivated in vitro for 24 h were used for transfer experiments. The transfer efficiency was estimated within 27 and 29 days according to the histological picture of the testes and the isolated cultures. Spermatogenic cells at various developmental stages and a few Sertoli ells and spermatozoa were found on sections and in cell suspensions. Sperm samples could be taken from recipient boars within nine months after the transfer. Microsatellite analysis of DNA showed the endogenous pattern of spermatogenesis. Thus, it was shown that spermatogenic donor cells can restore and maintain spermatogenesis of a recipient for at least 30 days. However, the donor cells were fully forced by the recipient reserve cells, type A0 spermatogonia, within eight to nine months.
    MeSH term(s) Animals ; Busulfan/pharmacology ; Cell Differentiation/drug effects ; Cells, Cultured ; Immunosuppressive Agents/pharmacology ; Male ; Sertoli Cells/cytology ; Sperm Count ; Spermatogenesis ; Spermatogonia/cytology ; Spermatogonia/transplantation ; Swine
    Chemical Substances Immunosuppressive Agents ; Busulfan (G1LN9045DK)
    Language Russian
    Publishing date 2006-07
    Publishing country Russia (Federation)
    Document type English Abstract ; Journal Article
    ZDB-ID 207373-0
    ISSN 0475-1450
    ISSN 0475-1450
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  8. Article ; Online: Regulation of Sertoli-germ cell adhesion and sperm release by FSH and nonclassical testosterone signaling.

    Shupe, John / Cheng, Jing / Puri, Pawan / Kostereva, Nataliya / Walker, William H

    Molecular endocrinology (Baltimore, Md.)

    2010  Volume 25, Issue 2, Page(s) 238–252

    Abstract: Testosterone and FSH act in synergy to produce the factors required to maximize the production of spermatozoa and male fertility. However, the molecular mechanisms by which these hormones support spermatogenesis are not well established. Recently, we ... ...

    Abstract Testosterone and FSH act in synergy to produce the factors required to maximize the production of spermatozoa and male fertility. However, the molecular mechanisms by which these hormones support spermatogenesis are not well established. Recently, we identified a nonclassical mechanism of testosterone signaling in cultured rat Sertoli cells. We found that testosterone binding to the androgen receptor recruits and activates Src tyrosine kinase. Src then causes the activation of the epidermal growth factor receptor, which results in the phosphorylation and activation of the ERK MAPK and the cAMP response element-binding protein transcription factor. In this report, we find that FSH inhibits testosterone-mediated activation of ERK and the MAPK pathway in Sertoli cells via the protein kinase A-mediated inhibition of Raf kinase. In addition, FSH, as well as inhibitors of Src and ERK kinase activity, reduced germ cell attachment to Sertoli cells in culture. Using pathway-specific androgen receptor mutants we found that the nonclassical pathway is required for testosterone-mediated increases in germ cell attachment to Sertoli cells. Studies of seminiferous tubule explants determined that Src kinase, but not ERK kinase, activity is required for the release of sperm from seminiferous tubule explants. These findings suggest the nonclassical testosterone-signaling pathway acts via Src and ERK kinases to facilitate the adhesion of immature germ cells to Sertoli cells and through Src to permit the release of mature spermatozoa. In contrast, FSH acts to limit testosterone-mediated ERK kinase activity and germ cell attachment.
    MeSH term(s) Animals ; Cell Adhesion ; Cells, Cultured ; Cyclic AMP-Dependent Protein Kinases/metabolism ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Follicle Stimulating Hormone/metabolism ; Male ; Phosphorylation ; Promoter Regions, Genetic/genetics ; Rats ; Seminiferous Tubules ; Sertoli Cells/physiology ; Signal Transduction ; Spermatogenesis ; Spermatozoa/physiology ; Testosterone/genetics ; Testosterone/metabolism ; raf Kinases/metabolism ; src-Family Kinases/metabolism
    Chemical Substances Testosterone (3XMK78S47O) ; Follicle Stimulating Hormone (9002-68-0) ; src-Family Kinases (EC 2.7.10.2) ; raf Kinases (EC 2.7.11.1) ; Cyclic AMP-Dependent Protein Kinases (EC 2.7.11.11) ; Extracellular Signal-Regulated MAP Kinases (EC 2.7.11.24)
    Language English
    Publishing date 2010-12-22
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 639167-9
    ISSN 1944-9917 ; 0888-8809
    ISSN (online) 1944-9917
    ISSN 0888-8809
    DOI 10.1210/me.2010-0030
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    Zs.A 2261: Show issues Location:
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  9. Article ; Online: Pyrolysis of Polyacrylonitrile/Technical Hydrolytic Lignin Composites

    Y. Sazanov / D. Kosyakov / S. Krutov / T. Kostereva / Y. Kulikova / N. Shkaeva / A. Ladesov / Y. Ipatova / S. Pokryshkin / G. Fedorova

    Eurasian Chemico-Technological Journal, Vol 17, Iss 4, Pp 287-

    2015  Volume 294

    Abstract: One important problem is utilization of technical hydrolytic lignin (the waste formed in paper-and-pulp and hydrolysis industry). For a practical implementation, the essential task of transforming insoluble hydrolytic lignin into low molecular weight ... ...

    Abstract One important problem is utilization of technical hydrolytic lignin (the waste formed in paper-and-pulp and hydrolysis industry). For a practical implementation, the essential task of transforming insoluble hydrolytic lignin into low molecular weight products with high degree of functionalization should be performed. In prospect, these products can serve as raw materials for synthesis of various organic compounds demanded in chemical industry. Among other things, activation and fragmentation of hydrolytic lignin yields low molecular weight compounds which may be used for modifying synthetic polymers (polymer-analogous transformations). In the present work, the search for the optimal solvents (activators) for technical hydrolytic lignin (THL) has been conducted; the dimethyl sulfoxide/water binary mixture proved to be the best solvent. Methods of thermal analysis (thermogravimetric analysis, TGA; differential thermal analysis, DTA; differential scanning calorimetry, DSC; thermal volumetric analysis, TVA) combined with pyrolysis-gas chromatography/mass spectrometry (GC-MS) were used to determine the grades of technical hydrolytic lignin most suitable for activation and fragmentation. The necessary conditions for thermal treatment of lignin samples and concentrations of initial compounds (lignin and polyacrylonitrile, PAN) in the binary solvent mixture (dimethyl sulfoxide/water) facilitating maximum THL fragmentation and its successful interaction with PAN were found. When using the dimethylsulfoxide-water binary mixture (70:30 mass ratio) as a solvent, homogeneous forming solution of initial components (THL-PAN) was prepared. With the use of syringe method, form-stable fibers with a maximum lignin content of 80% and strength of about 50 MPa were obtained. Analytical pyrolysis of composites (products of THL-PAN interaction in the binary solvent) allowed us to suggest a mechanism for THL fragmentation involving the binary solvent.
    Keywords Chemistry ; QD1-999
    Subject code 660
    Language English
    Publishing date 2015-11-01T00:00:00Z
    Publisher al-Farabi Kazakh National University
    Document type Article ; Online
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  10. Article ; Online: Abnormal Vessel Architecture Persists in the Microvasculature of the Massive Weight Loss Patient.

    Katzel, Evan B / Shakir, Sameer / Kostereva, Nataliya / Lannau, Bernd / Gimbel, Michael / Nguyen, Vu T / De La Cruz, Carolyn / Marra, Kacey / Gusenoff, Jeffrey A

    Plastic and reconstructive surgery

    2015  Volume 137, Issue 1, Page(s) 24e–30e

    Abstract: ... for intimal proliferation and luminal occlusion ratio.: Results: All patients (n = 23) were female ...

    Abstract Background: Research demonstrates a link between obesity and increased circulating inflammatory cytokines, which lead to changes in the microvasculature. Massive weight loss patients often experience delayed wound healing after body-contouring procedures; however, no studies exist to explore the inflammatory response of massive weight loss on microvasculature. This study hypothesized that massive weight loss patients who undergo body-contouring procedures maintain persistently elevated inflammatory markers in the microvasculature that delay wound healing.
    Methods: Superficial inferior epigastric artery vessels were harvested during abdominally based free flap surgery and abdominal contouring surgery for normal weight and massive weight loss patients, respectively. Vessels were histologically assessed using immunohistochemistry and trichome staining to assess and compare vessel architecture. Analysis was performed for intimal proliferation and luminal occlusion ratio.
    Results: All patients (n = 23) were female. Quantitative analysis of immunohistochemistry stains revealed no difference between normal weight and massive weight loss patients. Trichrome staining demonstrated abnormal vessel architecture in the massive weight loss group. Intimal proliferation was 11.4 ± 4.8 percent for normal weight patients compared with 29.5 ± 4.9 percent for massive weight loss patients (p < 0.0001). Occlusion ratio for normal weight patients was 29.9 ± 3.9 percent compared with 46.2 ± 8.1 percent for massive weight loss patients (p < 0.0001) CONCLUSIONS:: Despite the return to normal levels of inflammatory markers after massive weight loss, trichrome staining demonstrated irregular composition in the tunica adventitia and tunica media and increased intimal proliferation and occlusion ratio. This suggests vasculopathy that could explain delayed wound healing in the massive weight loss population.
    MeSH term(s) Abdominoplasty/methods ; Adult ; Bariatric Surgery/methods ; Biopsy, Needle ; Body Mass Index ; Case-Control Studies ; Epigastric Arteries/pathology ; Epigastric Arteries/surgery ; Female ; Follow-Up Studies ; Humans ; Immunohistochemistry ; Microvessels/abnormalities ; Microvessels/pathology ; Middle Aged ; Obesity, Morbid/surgery ; Reference Values ; Retrospective Studies ; Risk Assessment ; Tissue and Organ Harvesting ; Treatment Outcome ; Weight Loss
    Language English
    Publishing date 2015-11-21
    Publishing country United States
    Document type Comparative Study ; Journal Article
    ZDB-ID 208012-6
    ISSN 1529-4242 ; 0032-1052 ; 0096-8501
    ISSN (online) 1529-4242
    ISSN 0032-1052 ; 0096-8501
    DOI 10.1097/PRS.0000000000001905
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