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Article ; Online: Angiocrine Signaling in Sinusoidal Health and Disease.

Cooper, Shawna A / Kostallari, Enis / Shah, Vijay H

2023 Volume 43, Issue 3, Page(s) 245–257

Abstract: Liver sinusoidal endothelial cells (LSECs) are key players in maintaining hepatic homeostasis. They also play crucial roles during liver injury by communicating with liver cell types as well as immune cells and promoting portal hypertension, fibrosis, ... ...

Abstract	Liver sinusoidal endothelial cells (LSECs) are key players in maintaining hepatic homeostasis. They also play crucial roles during liver injury by communicating with liver cell types as well as immune cells and promoting portal hypertension, fibrosis, and inflammation. Cutting-edge technology, such as single cell and spatial transcriptomics, have revealed the existence of distinct LSEC subpopulations with a clear zonation in the liver. The signals released by LSECs are commonly called "angiocrine signaling." In this review, we summarize the role of angiocrine signaling in health and disease, including zonation in healthy liver, regeneration, fibrosis, portal hypertension, nonalcoholic fatty liver disease, alcohol-associated liver disease, aging, drug-induced liver injury, and ischemia/reperfusion, as well as potential therapeutic advances. In conclusion, sinusoidal endotheliopathy is recognized in liver disease and promising preclinical studies are paving the path toward LSEC-specific pharmacotherapies.
MeSH term(s)	Humans ; Endothelial Cells/metabolism ; Liver/pathology ; Non-alcoholic Fatty Liver Disease/metabolism ; Hypertension, Portal/metabolism ; Fibrosis ; Liver Cirrhosis/metabolism
Language	English
Publishing date	2023-07-13
Publishing country	United States
Document type	Review ; Journal Article
ZDB-ID	603177-8
ISSN	1098-8971 ; 0272-8087
ISSN (online)	1098-8971
ISSN	0272-8087
DOI	10.1055/a-2128-5907
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Omics and AI advance biomarker discovery for liver disease.

Wu, Tiffany / Cooper, Shawna A / Shah, Vijay H

Nature medicine

2022 Volume 28, Issue 6, Page(s) 1131–1132

MeSH term(s)	Artificial Intelligence ; Biomarkers ; Genomics ; Humans ; Liver Diseases/genetics ; Proteomics
Chemical Substances	Biomarkers
Language	English
Publishing date	2022-06-16
Publishing country	United States
Document type	Journal Article ; Comment
ZDB-ID	1220066-9
ISSN	1546-170X ; 1078-8956
ISSN (online)	1546-170X
ISSN	1078-8956
DOI	10.1038/s41591-022-01853-9
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Zs.A 4212: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article: Angiocrine Signaling in Sinusoidal Health and Disease

Cooper, Shawna A. / Kostallari, Enis / Shah, Vijay H.

Seminars in Liver Disease

2023 Volume 43, Issue 03, Page(s) 245–257

Abstract	Liver sinusoidal endothelial cells (LSECs) are key players in maintaining hepatic homeostasis. They also play crucial roles during liver injury by communicating with liver cell types as well as immune cells and promoting portal hypertension, fibrosis, and inflammation. Cutting-edge technology, such as single cell and spatial transcriptomics, have revealed the existence of distinct LSEC subpopulations with a clear zonation in the liver. The signals released by LSECs are commonly called “angiocrine signaling.” In this review, we summarize the role of angiocrine signaling in health and disease, including zonation in healthy liver, regeneration, fibrosis, portal hypertension, nonalcoholic fatty liver disease, alcohol-associated liver disease, aging, drug-induced liver injury, and ischemia/reperfusion, as well as potential therapeutic advances. In conclusion, sinusoidal endotheliopathy is recognized in liver disease and promising preclinical studies are paving the path toward LSEC-specific pharmacotherapies.
Keywords	angiocrine signaling ; liver sinusoidal endothelial cells ; liver health ; liver disease ; therapeutics
Language	English
Publishing date	2023-07-13
Publisher	Thieme Medical Publishers, Inc.
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	603177-8
ISSN	1098-8971 ; 0272-8087
ISSN (online)	1098-8971
ISSN	0272-8087
DOI	10.1055/a-2128-5907
Database	Thieme publisher's database

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Article ; Online: Three-Dimensional Structure of Inner Ear Hair Cell Ribbon Synapses in a Zebrafish Model of Usher Syndrome Type 1B.

Riley, Kenneth C / Koleilat, Alaa / Dugdale, Joseph A / Cooper, Shawna A / Christensen, Trace A / Schimmenti, Lisa A

Zebrafish

2023 Volume 20, Issue 2, Page(s) 47–54

Abstract: Our understanding of inner ear hair cell ultrastructure has heretofore relied upon two-dimensional imaging; however, serial block-face scanning electron microscopy (SBFSEM) changes this paradigm allowing for three-dimensional evaluation. We compared ... ...

Abstract	Our understanding of inner ear hair cell ultrastructure has heretofore relied upon two-dimensional imaging; however, serial block-face scanning electron microscopy (SBFSEM) changes this paradigm allowing for three-dimensional evaluation. We compared inner ear hair cells of the apical cristae in
MeSH term(s)	Animals ; Humans ; Zebrafish ; Usher Syndromes/genetics ; Usher Syndromes/metabolism ; Synapses/metabolism ; Synapses/ultrastructure ; Hair Cells, Auditory, Inner/metabolism ; Hair Cells, Auditory, Inner/ultrastructure ; Hair ; Myosins/genetics ; Myosins/metabolism ; Zebrafish Proteins/genetics ; Zebrafish Proteins/metabolism
Chemical Substances	Myo7aa protein, zebrafish ; Myosins (EC 3.6.4.1) ; Zebrafish Proteins
Language	English
Publishing date	2023-04-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2156020-1
ISSN	1557-8542 ; 1545-8547
ISSN (online)	1557-8542
ISSN	1545-8547
DOI	10.1089/zeb.2022.0049
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Pathologic light chain amyloidosis oligomer detection in urinary extracellular vesicles as a diagnostic tool for response and progression of disease.

Cooper, Shawna A / Dick, Christopher J / Misra, Pinaki / Leung, Nelson / Schinstock, Carrie A / Ramirez-Alvarado, Marina

Frontiers in oncology

2022 Volume 12, Page(s) 978198

Abstract: Light Chain (AL) Amyloidosis is a plasma cell dyscrasia producing amyloidogenic light chains (LC) that misfold and form amyloid deposits that cause damage in vital organs, primarily the heart and kidneys. Urinary extracellular vesicles (uEVs) are ... ...

Abstract	Light Chain (AL) Amyloidosis is a plasma cell dyscrasia producing amyloidogenic light chains (LC) that misfold and form amyloid deposits that cause damage in vital organs, primarily the heart and kidneys. Urinary extracellular vesicles (uEVs) are nanoparticles produced by renal epithelial cells throughout the nephron. We previously showed that uEVs from active renal AL amyloidosis patients contain LC oligomers that are large (>250kDa), resistant to heat and chemical denaturation, but of low abundance. Renal dysfunction in AL amyloidosis results in high urine protein, compounding technical challenges to use uEVs as analytical tools. In this study, we assess the use of uEVs as analytical diagnostic tools for response and disease progression in AL amyloidosis. Our results suggest that uEV protein concentration, urine volume, and particle concentrations are not directly correlated. Multiple strategies for overcoming non-specific antibody binding in uEV samples were validated in our study. We demonstrated that the sensitivity for pre-clinical testing is improved with a urine sample requirement algorithm that we developed. The findings of our study will provide a pathway toward development of critically needed tools for patient management. Sensitive detection of LC oligomers from a non-invasive urine sample rather than an invasive renal biopsy will reduce patient burden and healthcare costs. The ability to detect LC oligomers in patients with renal progression, despite positive hematologic response; will allow clinicians to confidently treat, but not overtreat, patients at risk of ongoing significant renal injury.
Language	English
Publishing date	2022-10-04
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2022.978198
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Article ; Online: Erratum. TFAM Enhances Fat Oxidation and Attenuates High-Fat Diet-Induced Insulin Resistance in Skeletal Muscle. Diabetes 2019;68:1552-1564.

Koh, Jin-Ho / Johnson, Matthew L / Dasari, Surendra / LeBrasseur, Nathan K / Vuckovic, Ivan / Henderson, Gregory C / Cooper, Shawna A / Manjunatha, Shankarappa / Ruegsegger, Gregory N / Shulman, Gerald I / Lanza, Ian R / Nair, K Sreekumaran

Diabetes

2020 Volume 69, Issue 8, Page(s) 1854

Language	English
Publishing date	2020-06-12
Publishing country	United States
Document type	Journal Article ; Published Erratum
ZDB-ID	80085-5
ISSN	1939-327X ; 0012-1797
ISSN (online)	1939-327X
ISSN	0012-1797
DOI	10.2337/db20-er08a
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Article ; Online: Stiffness is associated with hepatic stellate cell heterogeneity during liver fibrosis.

Kostallari, Enis / Wei, Bo / Sicard, Delphine / Li, Jiahui / Cooper, Shawna A / Gao, Jinhang / Dehankar, Mrunal / Li, Ying / Cao, Sheng / Yin, Meng / Tschumperlin, Daniel J / Shah, Vijay H

American journal of physiology. Gastrointestinal and liver physiology

2021 Volume 322, Issue 2, Page(s) G234–G246

Abstract: The fibrogenic wound-healing response in liver increases stiffness. Stiffness mechanotransduction, in turn, amplifies fibrogenesis. Here, we aimed to understand the distribution of stiffness in fibrotic liver, how it impacts hepatic stellate cell (HSC) ... ...

Abstract	The fibrogenic wound-healing response in liver increases stiffness. Stiffness mechanotransduction, in turn, amplifies fibrogenesis. Here, we aimed to understand the distribution of stiffness in fibrotic liver, how it impacts hepatic stellate cell (HSC) heterogeneity, and identify mechanisms by which stiffness amplifies fibrogenic responses. Magnetic resonance elastography and atomic force microscopy demonstrated a heterogeneous distribution of liver stiffness at macroscopic and microscopic levels, respectively, in a carbon tetrachloride (CCl
MeSH term(s)	Animals ; Carbon Tetrachloride/metabolism ; Cells, Cultured ; Disease Models, Animal ; Hepatic Stellate Cells/metabolism ; Humans ; Kupffer Cells/metabolism ; Liver/metabolism ; Liver Cirrhosis/metabolism ; Mechanotransduction, Cellular/physiology ; Mice
Chemical Substances	Carbon Tetrachloride (CL2T97X0V0)
Language	English
Publishing date	2021-12-23
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	603840-2
ISSN	1522-1547 ; 0193-1857
ISSN (online)	1522-1547
ISSN	0193-1857
DOI	10.1152/ajpgi.00254.2021
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Article ; Online: Polyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for SARS-CoV-2 testing.

Padgett, Leah R / Kennington, Lauren A / Ahls, Charlotte L / Samarasinghe, Delini K / Tu, Yuan-Po / Wallander, Michelle L / Cooper, Shawna D / Elliott, James S / Rains, Douglas

PloS one

2021 Volume 16, Issue 4, Page(s) e0245423

Abstract: Background: In order to identify an inexpensive yet highly stable SARS-CoV-2 collection device as an alternative to foam swabs stored in transport media, both contrived ("surrogate") CoV-positive and patient-collected spun polyester swabs stored in dry ... ...

Abstract	Background: In order to identify an inexpensive yet highly stable SARS-CoV-2 collection device as an alternative to foam swabs stored in transport media, both contrived ("surrogate") CoV-positive and patient-collected spun polyester swabs stored in dry tubes were evaluated for time- and temperature-stability using qPCR. Methods: Surrogate specimens were prepared by combining multiple, residual SARS-CoV-2-positive clinical specimens and diluting to near-LOD levels in either porcine or human mucus ("matrix"), inoculating foam or polyester nasal swabs, and sealing in dry tubes. Swabs were then subjected to one of three temperature excursions: (1) 4°C for up to 72 hours; (2) 40°C for 12 hours, followed by 32°C for up to 60 hours; or (3) multiple freeze-thaw cycles (-20°C). The stability of extracted SARS-CoV-2 RNA for each condition was evaluated by qPCR. Separate usability studies for the dry polyester swab-based HealthPulse@home COVID-19 Specimen Collection Kit were later conducted in both adult and pediatric populations. Results: Polyester swabs stored dry demonstrated equivalent performance to foam swabs for detection of low and moderate SARS-CoV-2 viral loads. Mimicking warm- and cold- climate shipment, surrogate specimens were stable following either 72 hours of a high-temperature excursion or two freeze-thaw cycles. In addition, usability studies comprised of self-collected patient specimens yielded sufficient material for molecular testing, as demonstrated by RNase P detection. Conclusions: Polyester nasal swabs stored in dry collection tubes offer a robust and inexpensive self-collection method for SARS-CoV-2 viral load testing, as viral RNA remains stable under conditions required for home collection and shipment to the laboratory.
MeSH term(s)	Animals ; COVID-19/diagnosis ; COVID-19/virology ; COVID-19 Testing/methods ; Clinical Laboratory Techniques/methods ; Diagnostic Tests, Routine/methods ; Humans ; Molecular Diagnostic Techniques ; Nasopharynx/virology ; Polyesters ; RNA, Viral/genetics ; Real-Time Polymerase Chain Reaction/methods ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Specimen Handling/methods ; Swine
Chemical Substances	Polyesters ; RNA, Viral
Language	English
Publishing date	2021-04-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0245423
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Polyester nasal swabs collected in a dry tube are a robust and inexpensive, minimal self-collection kit for SARS-CoV-2 testing.

Leah R Padgett / Lauren A Kennington / Charlotte L Ahls / Delini K Samarasinghe / Yuan-Po Tu / Michelle L Wallander / Shawna D Cooper / James S Elliott / Douglas Rains

PLoS ONE, Vol 16, Iss 4, p e

2021 Volume 0245423

Abstract: Background In order to identify an inexpensive yet highly stable SARS-CoV-2 collection device as an alternative to foam swabs stored in transport media, both contrived ("surrogate") CoV-positive and patient-collected spun polyester swabs stored in dry ... ...

Abstract	Background In order to identify an inexpensive yet highly stable SARS-CoV-2 collection device as an alternative to foam swabs stored in transport media, both contrived ("surrogate") CoV-positive and patient-collected spun polyester swabs stored in dry tubes were evaluated for time- and temperature-stability using qPCR. Methods Surrogate specimens were prepared by combining multiple, residual SARS-CoV-2-positive clinical specimens and diluting to near-LOD levels in either porcine or human mucus ("matrix"), inoculating foam or polyester nasal swabs, and sealing in dry tubes. Swabs were then subjected to one of three temperature excursions: (1) 4°C for up to 72 hours; (2) 40°C for 12 hours, followed by 32°C for up to 60 hours; or (3) multiple freeze-thaw cycles (-20°C). The stability of extracted SARS-CoV-2 RNA for each condition was evaluated by qPCR. Separate usability studies for the dry polyester swab-based HealthPulse@home COVID-19 Specimen Collection Kit were later conducted in both adult and pediatric populations. Results Polyester swabs stored dry demonstrated equivalent performance to foam swabs for detection of low and moderate SARS-CoV-2 viral loads. Mimicking warm- and cold- climate shipment, surrogate specimens were stable following either 72 hours of a high-temperature excursion or two freeze-thaw cycles. In addition, usability studies comprised of self-collected patient specimens yielded sufficient material for molecular testing, as demonstrated by RNase P detection. Conclusions Polyester nasal swabs stored in dry collection tubes offer a robust and inexpensive self-collection method for SARS-CoV-2 viral load testing, as viral RNA remains stable under conditions required for home collection and shipment to the laboratory.
Keywords	Medicine ; R ; Science ; Q
Subject code	600
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Assays for Light Chain Amyloidosis Formation and Cytotoxicity.

Blancas-Mejia, Luis M / Misra, Pinaki / Dick, Christopher J / Marin-Argany, Marta / Redhage, Keely R / Cooper, Shawna A / Ramirez-Alvarado, Marina

Methods in molecular biology (Clifton, N.J.)

2018 Volume 1873, Page(s) 123–153

Abstract: Common biophysical techniques like absorption and fluorescence spectroscopy, microscopy, and light scattering studies have been in use to investigate fibril assembly for a long time. However, there is sometimes a lack of consensus from the findings of an ...

Abstract	Common biophysical techniques like absorption and fluorescence spectroscopy, microscopy, and light scattering studies have been in use to investigate fibril assembly for a long time. However, there is sometimes a lack of consensus from the findings of an individual technique when compared in parallel with the other techniques. In this chapter, we aim to provide a concise compilation of techniques that can effectively be used to obtain a comprehensive representation of the structural, aggregation, and toxicity determinants in immunoglobulin light chain amyloidosis. We start by giving a brief introduction on amyloid assembly and the advantages of using simple and readily available techniques to study aggregation. After an overview on preparation of protein to set up parallel experiments, we provide a systematic description of the in vitro techniques used to study aggregation in AL protein. Additionally, we thoroughly discuss the steps needed in our experience during the individual experiments for better reproducibility and data analysis.
MeSH term(s)	Amyloid/chemistry ; Amyloid/metabolism ; Amyloidogenic Proteins/chemistry ; Amyloidogenic Proteins/metabolism ; Amyloidosis/diagnosis ; Apoptosis ; Benzothiazoles/chemistry ; Benzothiazoles/metabolism ; Biological Assay/methods ; Chromatography, Gel ; Chromatography, High Pressure Liquid ; Circular Dichroism ; Dynamic Light Scattering ; Immunoglobulin Light Chains/chemistry ; Immunoglobulin Light Chains/metabolism ; Particle Size ; Spectrometry, Fluorescence
Chemical Substances	Amyloid ; Amyloidogenic Proteins ; Benzothiazoles ; Immunoglobulin Light Chains ; thioflavin T (2390-54-7)
Language	English
Publishing date	2018-10-19
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-8820-4_8
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