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  1. Article: DDK: The Outsourced Kinase of Chromosome Maintenance.

    Gillespie, Peter J / Blow, J Julian

    Biology

    2022  Volume 11, Issue 6

    Abstract: The maintenance of genomic stability during the mitotic cell-cycle not only demands that the DNA is duplicated and repaired with high fidelity, but that following DNA replication the chromatin composition is perpetuated and that the duplicated chromatids ...

    Abstract The maintenance of genomic stability during the mitotic cell-cycle not only demands that the DNA is duplicated and repaired with high fidelity, but that following DNA replication the chromatin composition is perpetuated and that the duplicated chromatids remain tethered until their anaphase segregation. The coordination of these processes during S phase is achieved by both cyclin-dependent kinase, CDK, and Dbf4-dependent kinase, DDK. CDK orchestrates the activation of DDK at the G1-to-S transition, acting as the 'global' regulator of S phase and cell-cycle progression, whilst 'local' control of the initiation of DNA replication and repair and their coordination with the re-formation of local chromatin environments and the establishment of chromatid cohesion are delegated to DDK. Here, we discuss the regulation and the multiple roles of DDK in ensuring chromosome maintenance. Regulation of replication initiation by DDK has long been known to involve phosphorylation of MCM2-7 subunits, but more recent results have indicated that Treslin:MTBP might also be important substrates. Molecular mechanisms by which DDK regulates replisome stability and replicated chromatid cohesion are less well understood, though important new insights have been reported recently. We discuss how the 'outsourcing' of activities required for chromosome maintenance to DDK allows CDK to maintain outright control of S phase progression and the cell-cycle phase transitions whilst permitting ongoing chromatin replication and cohesion establishment to be completed and achieved faithfully.
    Language English
    Publishing date 2022-06-07
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2661517-4
    ISSN 2079-7737
    ISSN 2079-7737
    DOI 10.3390/biology11060877
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Defects in the origin licensing checkpoint stresses cells exiting G0.

    Blow, J Julian

    The Journal of cell biology

    2019  Volume 218, Issue 7, Page(s) 2080–2081

    Abstract: The full licensing of replication origins in late G1 is normally enforced by the licensing checkpoint. In this issue, Matson et al. (2019. ...

    Abstract The full licensing of replication origins in late G1 is normally enforced by the licensing checkpoint. In this issue, Matson et al. (2019.
    MeSH term(s) Cell Cycle ; Cell Cycle Proteins ; Cell Division ; DNA Replication ; Replication Origin
    Chemical Substances Cell Cycle Proteins
    Language English
    Publishing date 2019-06-11
    Publishing country United States
    Document type Journal Article ; Comment
    ZDB-ID 218154-x
    ISSN 1540-8140 ; 0021-9525
    ISSN (online) 1540-8140
    ISSN 0021-9525
    DOI 10.1083/jcb.201905181
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: DDK

    Peter J. Gillespie / J. Julian Blow

    Biology, Vol 11, Iss 877, p

    The Outsourced Kinase of Chromosome Maintenance

    2022  Volume 877

    Abstract: The maintenance of genomic stability during the mitotic cell-cycle not only demands that the DNA is duplicated and repaired with high fidelity, but that following DNA replication the chromatin composition is perpetuated and that the duplicated chromatids ...

    Abstract The maintenance of genomic stability during the mitotic cell-cycle not only demands that the DNA is duplicated and repaired with high fidelity, but that following DNA replication the chromatin composition is perpetuated and that the duplicated chromatids remain tethered until their anaphase segregation. The coordination of these processes during S phase is achieved by both cyclin-dependent kinase, CDK, and Dbf4-dependent kinase, DDK. CDK orchestrates the activation of DDK at the G1-to-S transition, acting as the ‘global’ regulator of S phase and cell-cycle progression, whilst ‘local’ control of the initiation of DNA replication and repair and their coordination with the re-formation of local chromatin environments and the establishment of chromatid cohesion are delegated to DDK. Here, we discuss the regulation and the multiple roles of DDK in ensuring chromosome maintenance. Regulation of replication initiation by DDK has long been known to involve phosphorylation of MCM2-7 subunits, but more recent results have indicated that Treslin:MTBP might also be important substrates. Molecular mechanisms by which DDK regulates replisome stability and replicated chromatid cohesion are less well understood, though important new insights have been reported recently. We discuss how the ‘outsourcing’ of activities required for chromosome maintenance to DDK allows CDK to maintain outright control of S phase progression and the cell-cycle phase transitions whilst permitting ongoing chromatin replication and cohesion establishment to be completed and achieved faithfully.
    Keywords DDK ; CDK ; DNA replication ; replication fork stability ; DNA repair ; chromatid cohesion ; Biology (General) ; QH301-705.5
    Subject code 612
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: The Atad5 RFC-like complex is the major unloader of proliferating cell nuclear antigen in Xenopus egg extracts.

    Kawasoe, Yoshitaka / Shimokawa, Sakiko / Gillespie, Peter J / Blow, J Julian / Tsurimoto, Toshiki / Takahashi, Tatsuro S

    The Journal of biological chemistry

    2023  Volume 300, Issue 1, Page(s) 105588

    Abstract: Proliferating cell nuclear antigen (PCNA) is a homo-trimeric clamp complex that serves as the molecular hub for various DNA transactions, including DNA synthesis and post-replicative mismatch repair. Its timely loading and unloading are critical for ... ...

    Abstract Proliferating cell nuclear antigen (PCNA) is a homo-trimeric clamp complex that serves as the molecular hub for various DNA transactions, including DNA synthesis and post-replicative mismatch repair. Its timely loading and unloading are critical for genome stability. PCNA loading is catalyzed by Replication factor C (RFC) and the Ctf18 RFC-like complex (Ctf18-RLC), and its unloading is catalyzed by Atad5/Elg1-RLC. However, RFC, Ctf18-RLC, and even some subcomplexes of their shared subunits are capable of unloading PCNA in vitro, leaving an ambiguity in the division of labor in eukaryotic clamp dynamics. By using a system that specifically detects PCNA unloading, we show here that Atad5-RLC, which accounts for only approximately 3% of RFC/RLCs, nevertheless provides the major PCNA unloading activity in Xenopus egg extracts. RFC and Ctf18-RLC each account for approximately 40% of RFC/RLCs, while immunodepletion of neither Rfc1 nor Ctf18 detectably affects the rate of PCNA unloading in our system. PCNA unloading is dependent on the ATP-binding motif of Atad5, independent of nicks on DNA and chromatin assembly, and inhibited effectively by PCNA-interacting peptides. These results support a model in which Atad5-RLC preferentially unloads DNA-bound PCNA molecules that are free from their interactors.
    MeSH term(s) Animals ; DNA ; DNA Replication ; Proliferating Cell Nuclear Antigen/genetics ; Proliferating Cell Nuclear Antigen/metabolism ; Replication Protein C/genetics ; Replication Protein C/metabolism ; Xenopus laevis/metabolism ; Oocytes ; ATPases Associated with Diverse Cellular Activities/genetics ; ATPases Associated with Diverse Cellular Activities/metabolism ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism
    Chemical Substances DNA (9007-49-2) ; Proliferating Cell Nuclear Antigen ; Replication Protein C (EC 3.6.4.-) ; ATPases Associated with Diverse Cellular Activities (EC 3.6.4.-) ; DNA-Binding Proteins
    Language English
    Publishing date 2023-12-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    DOI 10.1016/j.jbc.2023.105588
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  5. Article: The location and development of Replicon Cluster Domains in early replicating DNA.

    da Costa-Nunes, José A / Gierlinski, Marek / Sasaki, Takayo / Haagensen, Emma J / Gilbert, David M / Blow, J Julian

    Wellcome open research

    2023  Volume 8, Page(s) 158

    Abstract: ... ...

    Abstract Background
    Language English
    Publishing date 2023-08-22
    Publishing country England
    Document type Journal Article
    ISSN 2398-502X
    ISSN 2398-502X
    DOI 10.12688/wellcomeopenres.18742.2
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  6. Book: Eukaryotic DNA replication

    Blow, J. Julian

    (Frontiers in molecular biology ; 15)

    1996  

    Author's details ed. by J. Julian Blow
    Series title Frontiers in molecular biology ; 15
    Collection
    Keywords DNA Replication ; DNS ; Replikation
    Subject Autoreduplikation ; Reduplikation ; DNS-Replikation ; RNS-Replikation ; DNA-Replikation ; RNA-Replikation ; Desoxyribonucleinsäure ; DNA ; DNA-Molekül ; Desoxyribonukleinsäure
    Language English
    Size XIX, 232 S. : Ill.
    Publisher IRL Press
    Publishing place Oxford u.a.
    Publishing country Great Britain
    Document type Book
    HBZ-ID HT007412500
    ISBN 0-19-963586-2 ; 0-19-963585-4 ; 978-0-19-963586-3 ; 978-0-19-963585-6
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    1998 A 3621 order for loan Magazin

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  7. Article: DDK: The Outsourced Kinase of Chromosome Maintenance

    Gillespie, Peter J. / Blow, J. Julian

    Biology. 2022 June 07, v. 11, no. 6

    2022  

    Abstract: The maintenance of genomic stability during the mitotic cell-cycle not only demands that the DNA is duplicated and repaired with high fidelity, but that following DNA replication the chromatin composition is perpetuated and that the duplicated chromatids ...

    Abstract The maintenance of genomic stability during the mitotic cell-cycle not only demands that the DNA is duplicated and repaired with high fidelity, but that following DNA replication the chromatin composition is perpetuated and that the duplicated chromatids remain tethered until their anaphase segregation. The coordination of these processes during S phase is achieved by both cyclin-dependent kinase, CDK, and Dbf4-dependent kinase, DDK. CDK orchestrates the activation of DDK at the G1-to-S transition, acting as the ‘global’ regulator of S phase and cell-cycle progression, whilst ‘local’ control of the initiation of DNA replication and repair and their coordination with the re-formation of local chromatin environments and the establishment of chromatid cohesion are delegated to DDK. Here, we discuss the regulation and the multiple roles of DDK in ensuring chromosome maintenance. Regulation of replication initiation by DDK has long been known to involve phosphorylation of MCM2-7 subunits, but more recent results have indicated that Treslin:MTBP might also be important substrates. Molecular mechanisms by which DDK regulates replisome stability and replicated chromatid cohesion are less well understood, though important new insights have been reported recently. We discuss how the ‘outsourcing’ of activities required for chromosome maintenance to DDK allows CDK to maintain outright control of S phase progression and the cell-cycle phase transitions whilst permitting ongoing chromatin replication and cohesion establishment to be completed and achieved faithfully.
    Keywords DNA ; DNA replication ; anaphase ; chromatids ; chromatin ; cohesion ; cyclin-dependent kinase ; genomics ; interphase ; mitosis ; phosphorylation
    Language English
    Dates of publication 2022-0607
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2661517-4
    ISSN 2079-7737
    ISSN 2079-7737
    DOI 10.3390/biology11060877
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: The role of DDK and Treslin-MTBP in coordinating replication licensing and pre-initiation complex formation.

    Volpi, Ilaria / Gillespie, Peter J / Chadha, Gaganmeet Singh / Blow, J Julian

    Open biology

    2021  Volume 11, Issue 10, Page(s) 210121

    Abstract: Treslin/Ticrr is required for the initiation of DNA replication and binds to MTBP (Mdm2 Binding Protein). Here, we show that ... ...

    Abstract Treslin/Ticrr is required for the initiation of DNA replication and binds to MTBP (Mdm2 Binding Protein). Here, we show that in
    MeSH term(s) Animals ; Carrier Proteins/metabolism ; Cell Cycle Proteins/metabolism ; Chromatin/metabolism ; Cyclin-Dependent Kinases/metabolism ; DNA Replication ; Female ; Gene Expression Regulation ; Male ; Protein Multimerization ; Protein Serine-Threonine Kinases/metabolism ; S Phase ; Xenopus Proteins/metabolism ; Xenopus laevis/genetics ; Xenopus laevis/metabolism
    Chemical Substances Carrier Proteins ; Cell Cycle Proteins ; Chromatin ; TICRR protein, Xenopus ; Xenopus Proteins ; CDC7 protein, Xenopus (EC 2.7.1.-) ; Protein Serine-Threonine Kinases (EC 2.7.11.1) ; Cyclin-Dependent Kinases (EC 2.7.11.22)
    Language English
    Publishing date 2021-10-27
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2630944-0
    ISSN 2046-2441 ; 2046-2441
    ISSN (online) 2046-2441
    ISSN 2046-2441
    DOI 10.1098/rsob.210121
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Author Correction: Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans.

    Pelisch, Federico / Sonneville, Remi / Pourkarimi, Ehsan / Agostinho, Ana / Blow, J Julian / Gartner, Anton / Hay, Ronald T

    Nature communications

    2022  Volume 13, Issue 1, Page(s) 7220

    Language English
    Publishing date 2022-11-24
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-022-35079-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Xenopus cell-free extracts and their contribution to the study of DNA replication and other complex biological processes.

    Blow, J Julian / Laskey, Ronald A

    The International journal of developmental biology

    2016  Volume 60, Issue 7-8-9, Page(s) 201–207

    Abstract: Here we discuss the important contributions that cell-free extracts have made to the study of complex biological processes. We provide a brief history of how cell-free extracts of frog eggs were developed to avoid many of the problems that can arise from ...

    Abstract Here we discuss the important contributions that cell-free extracts have made to the study of complex biological processes. We provide a brief history of how cell-free extracts of frog eggs were developed to avoid many of the problems that can arise from the dilution and mixing of cellular components that typically occur when cell-free extracts are prepared. We briefly describe how Xenopus egg extracts have been fundamental to the study of many important cellular processes including DNA replication, cell cycle progression, nuclear protein import, nuclear assembly and chromosome organisation. We describe how, in particular, Xenopus egg extracts have made a major contributions to the study of DNA replication, by permitting the direct manipulation of proteins in a system that is extraordinarily faithful to the way that DNA replication occurs in the living embryo. Finally we consider how results obtained using Xenopus egg extracts are being translated to produce diagnostic reagents for cancer screening and diagnosis.
    MeSH term(s) Animals ; Cell Nucleus/metabolism ; Cell-Free System ; DNA Replication/physiology ; Oocytes/metabolism ; Xenopus
    Language English
    Publishing date 2016-10-18
    Publishing country Spain
    Document type Journal Article ; Review
    ZDB-ID 1036070-0
    ISSN 1696-3547 ; 0214-6282
    ISSN (online) 1696-3547
    ISSN 0214-6282
    DOI 10.1387/ijdb.160142jb
    Database MEDical Literature Analysis and Retrieval System OnLINE

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