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  1. Article ; Online: De novo assembly and annotation of the Zhe-Maidong (Ophiopogon japonicus (L.f.) Ker-Gawl) transcriptome in different growth stages.

    Liu, Huijun / Wang, Ying / Wang, Tingzhang / Ying, Xuhui / Wu, Rongrong / Chen, Huan

    Scientific reports

    2017  Volume 7, Issue 1, Page(s) 3616

    Abstract: Zhe-Maidong (Ophiopogon japonicus (L.f.) Ker-Gawl) is a traditional medicinal herb in the family ... three different growth stages of Zhe-Maidong were investigated using RNA-seq, and a total of 16.4 Gb ... for Zhe-Maidong and will expand the available O. japonicus gene library and facilitate further genome-wide ...

    Abstract Zhe-Maidong (Ophiopogon japonicus (L.f.) Ker-Gawl) is a traditional medicinal herb in the family Liliaceae that has significant pharmacological effects on immunity and cardiovascular disease. In this study, three different growth stages of Zhe-Maidong were investigated using RNA-seq, and a total of 16.4 Gb of raw data was obtained. After filtering and assembling, 96,738 unigenes with an average length of 605.3 bp were ultimately generated. A total of 77,300 unigenes were annotated using information from five databases, including the NT, NR, SwissProt, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases. Additionally, the mechanisms of flavonoid, saponin and polysaccharide biosynthesis and of accumulation at different stages of tuber development were also characterized. From the first to third years, the contents of flavonoids, saponins and polysaccharides all increased, whereas the expression levels of related genes decreased in the flavonoid and saponin pathways and first increased and then decreased in the polysaccharide pathway. The results of this study provide the most comprehensive expressed sequence resource for Zhe-Maidong and will expand the available O. japonicus gene library and facilitate further genome-wide research and analyses of this species.
    MeSH term(s) Asparagaceae/genetics ; Asparagaceae/growth & development ; Asparagaceae/metabolism ; Computational Biology/methods ; Flavonoids/metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Gene Ontology ; High-Throughput Nucleotide Sequencing ; Life Cycle Stages ; Metabolic Networks and Pathways ; Molecular Sequence Annotation ; Transcriptome
    Chemical Substances Flavonoids
    Language English
    Publishing date 2017-06-15
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-017-03937-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Book: WHO de Zhongguo shi zhe

    Chen, Hui

    Hu Qingli zhuan

    (Jie chu Jiao da ren cong shu)

    2007  

    Title variant Hu Qingli zhuan
    Institution World Health Organization
    Author's details Chen Hui zhu
    Series title Jie chu Jiao da ren cong shu
    MeSH term(s) Physicians
    Keywords China
    Language Chinese
    Size 26, 185 p., 26 p. of plates. :, ill. (some col.), ports ;, 23 cm.
    Edition Di 1 ban.
    Publisher Shanghai jiao tong da xue chu ban she
    Publishing place Shanghai
    Document type Book
    ISBN 9787313049858 ; 7313049854
    Database Catalogue of the US National Library of Medicine (NLM)

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  3. Article ; Online: De novo assembly and annotation of the Zhe-Maidong (Ophiopogon japonicus (L.f.) Ker-Gawl) transcriptome in different growth stages

    Huijun Liu / Ying Wang / Tingzhang Wang / Xuhui Ying / Rongrong Wu / Huan Chen

    Scientific Reports, Vol 7, Iss 1, Pp 1-

    2017  Volume 15

    Abstract: Abstract Zhe-Maidong (Ophiopogon japonicus (L.f.) Ker-Gawl) is a traditional medicinal herb ... In this study, three different growth stages of Zhe-Maidong were investigated using RNA-seq, and a total of 16.4 ... sequence resource for Zhe-Maidong and will expand the available O. japonicus gene library and facilitate ...

    Abstract Abstract Zhe-Maidong (Ophiopogon japonicus (L.f.) Ker-Gawl) is a traditional medicinal herb in the family Liliaceae that has significant pharmacological effects on immunity and cardiovascular disease. In this study, three different growth stages of Zhe-Maidong were investigated using RNA-seq, and a total of 16.4 Gb of raw data was obtained. After filtering and assembling, 96,738 unigenes with an average length of 605.3 bp were ultimately generated. A total of 77,300 unigenes were annotated using information from five databases, including the NT, NR, SwissProt, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases. Additionally, the mechanisms of flavonoid, saponin and polysaccharide biosynthesis and of accumulation at different stages of tuber development were also characterized. From the first to third years, the contents of flavonoids, saponins and polysaccharides all increased, whereas the expression levels of related genes decreased in the flavonoid and saponin pathways and first increased and then decreased in the polysaccharide pathway. The results of this study provide the most comprehensive expressed sequence resource for Zhe-Maidong and will expand the available O. japonicus gene library and facilitate further genome-wide research and analyses of this species.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2017-06-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: [Effects of compound Zhe-Bei granule (CZBG) combined with doxorubicin on expression of membrane transport proteins in K562/A02 cell xenografts].

    Li, Dong-Yun / Zheng, Zhi / Hou, Li / Jiang, Miao / Dong, Qing / Tian, Shao-Dan / Ma, Wei / Chen, Ju / Wang, Jing / Chen, Xin-Yi

    Zhongguo shi yan xue ye xue za zhi

    2010  Volume 18, Issue 1, Page(s) 45–48

    Abstract: This study was purposed to investigate the effects of compound Zhe-Bei granule (CZBG) combined ...

    Abstract This study was purposed to investigate the effects of compound Zhe-Bei granule (CZBG) combined with doxorubicin on expressions of P-gp, MRP, LRP in K562/A02 cell xenografts. Tumor xenograft model were established by injecting the multidrug resistant cell line K562/A02 in the axillary flank of BALB/c-nu-nu mice. CZBG-intragastric administration and doxorubicin-intraperitoneal injection in combination were given to the BALB/c-nu nude mice. The tumor xenografts were made into slice after the dissection, and the expression of P-gp, MRP, LRP in K562/A02 tumor xenografts in mice were investigated by immunohistochemical technique. The integral optical density (IOD) of P-gp, MRP, LRP in K562/A02 tumor xenografts were measured by Image Pro Plus 6.0. The results showed that as compared with the doxorubicin alone, the combination of the doxorubicin and CZBG with high, middle and low dosage could decrease IOD of P-gp, MRP in K562/A02 tumor xenografts with statistical significance (p < 0.05). The LRP expression in K562/A02 tumor xenografts was not observed in five groups. It is concluded that the combination of CZBG with doxorubicin decreases the expressions of P-gp, MRP in K562/A02 tumor xenografts of mice.
    MeSH term(s) Animals ; Doxorubicin/pharmacology ; Drug Resistance, Multiple/drug effects ; Drug Resistance, Neoplasm/drug effects ; Drugs, Chinese Herbal/pharmacology ; Female ; Gene Expression Regulation, Leukemic/drug effects ; Humans ; K562 Cells ; Membrane Transport Proteins/metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Xenograft Model Antitumor Assays
    Chemical Substances Drugs, Chinese Herbal ; Membrane Transport Proteins ; Doxorubicin (80168379AG)
    Language Chinese
    Publishing date 2010-02
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2404306-0
    ISSN 1009-2137
    ISSN 1009-2137
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6603: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Book: Taiwan fu chan ke de bo zhong zhe

    Chen, Anjun

    Chen Anjun hui yi lu

    (Wang chun feng zhuan ji cong kan ; 14)

    2005  

    Title variant Chen Anjun hui yi lu
    Author's details Chen Anjun kou shu ; Lin Xiuzhen zhi bi
    Series title Wang chun feng zhuan ji cong kan ; 14
    MeSH term(s) Physicians ; Gynecology/history ; History, 20th Century
    Keywords Taiwan
    Language Chinese
    Size 203 p., [30] p. of plates :, ill. (some col.), ports.
    Edition Chu ban.
    Publisher Wang chun feng wen hua chu ban ; Taiwan Pub
    Publishing place Taibei Shi ; Upland, Ca
    Document type Book
    ISBN 9789572989241 ; 9572989243
    Database Catalogue of the US National Library of Medicine (NLM)

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  6. Article: [Effect of compound Zhe-Bei granule (CZBG) combined with doxorubicin on cell apoptosis and expression of related proteins in K562/A02 multidrug resistance tumor xenografts in mice].

    Chen, Xin-Yi / Zheng, Zhi / Chen, Ju / Li, Dong Yun

    Zhongguo shi yan xue ye xue za zhi

    2009  Volume 17, Issue 6, Page(s) 1439–1442

    Abstract: This study was purposed to investigate the effects of compound Zhe-Bei granule (CZBG) combined ...

    Abstract This study was purposed to investigate the effects of compound Zhe-Bei granule (CZBG) combined with doxorubicin (Dox) on the expression of BCL-2, BAX and cell apoptosis rate in K562/A02 multidrug resistant tumor xenografts. Tumor xenograft model was established by injecting the K562/A02 cells into the axillary flank of BALB/c nude mice. The cell apoptosis rate of xenografts was detected by flow cytometry with Annexin V-FITC/PI staining. The expressions of BCL-2 and BAX in xenografts were measured by immunohistochemistry staining. The value of integral optical density (IOD) was calculated according to staining image on slide. The results indicated that as compared with normal saline control group and Dox group, the high, middle, low dose of CZBG combined with Dox could increase the cell apoptosis rate of tumor xenografts with statistical significance (p < 0.05); and could decrease the IOD value of BCL-2 and increase the IOD value of BAX in xenografts with statistical significance (p < 0.05). It is concluded that CZBG combined with Dox can enhance the apoptosis rate in K562/A02 cells, down-regulate the expression of BCL-2 and up-regulate the expression of BAX, thereby, can reverse the multidrug resistance of K562/A02 cells by decreasing the ratio of BCL-2/BAX.
    MeSH term(s) Animals ; Apoptosis/drug effects ; Doxorubicin/pharmacology ; Doxorubicin/therapeutic use ; Drug Resistance, Neoplasm/drug effects ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Female ; Humans ; K562 Cells ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Proto-Oncogene Proteins/metabolism ; Proto-Oncogene Proteins c-bcl-2 ; bcl-2-Associated X Protein/metabolism
    Chemical Substances Bax protein, mouse ; Drugs, Chinese Herbal ; Proto-Oncogene Proteins ; Proto-Oncogene Proteins c-bcl-2 ; bcl-2-Associated X Protein ; Bcl2 protein, mouse (114100-40-2) ; Doxorubicin (80168379AG)
    Language Chinese
    Publishing date 2009-12
    Publishing country China
    Document type English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2404306-0
    ISSN 1009-2137
    ISSN 1009-2137
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6603: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  7. Book ; Online ; E-Book: Paclitaxel

    Kumara Swamy, Mallappa / Pullaiah, T. / Chen, Zhe-Sheng

    sources, chemistry, anticancer actions, and current biotechnology

    2022  

    Author's details edited by T. Pullaiah, Mallappa Kumara Swamy, Zhe-Sheng Chen
    Keywords Paclitaxel ; Paclitaxel/Therapeutic use.
    Subject code 616.994061
    Language English
    Size 1 online resource (356 pages)
    Publisher Elsevier
    Publishing place Waltham, Massachusetts
    Document type Book ; Online ; E-Book
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    ISBN 0-323-90952-3 ; 0-323-90951-5 ; 978-0-323-90952-5 ; 978-0-323-90951-8
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

    Full text online

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  8. Article: Reproductive cycle and biochemical composition of the Zhe oyster Crassostrea plicatula Gmelin in an eastern coastal bay of China

    Li, Q / Liu, W / Shirasu, K / Chen, W / Jiang, S

    Aquaculture. 2006 Nov. 24, v. 261, issue 2

    2006  

    Abstract: The reproductive cycle and biochemical composition of the oyster Crassostrea plicatula in Rushan Bay, an eastern coastal bay of China, were investigated between March 2004 and February 2005 in relation to environmental factors (temperature, salinity and ... ...

    Abstract The reproductive cycle and biochemical composition of the oyster Crassostrea plicatula in Rushan Bay, an eastern coastal bay of China, were investigated between March 2004 and February 2005 in relation to environmental factors (temperature, salinity and chlorophyll a). Histological analysis, combined with oocyte examination and measurements of protein, glycogen and lipid levels and RNA/DNA ratio from gonad, adductor muscle and mantle tissue of each sex were performed. The gametogenic cycle of C. plicatula comprised two phases: a resting phase (November-February) and reproductively active phase, including ripeness and spawning, during the rest of year. Gametogenesis in spring and summer was associated with an increase in protein and lipid contents in the ovaries, and took place at the expense of reserves (mainly glycogen) accumulated previously during winter. The RNA/DNA ratio is a good indicator of sexual maturity in the ovary; the increasing RNA/DNA ratio in the ovary appears to show the rising synthetic activity of vitellin within the ovary. Spawning occurred at the moment when water temperature and chlorophyll a levels were highest, and salinity lowest throughout the year. High variation in glycogen contents during storage and gametogenic development suggests that carbohydrates are the main respiratory substrate in this species. The results demonstrated that C. plicatula may be considered a conservative species in gametogenic pattern.
    Keywords Crassostrea ; oysters ; animal reproduction ; gametogenesis ; body composition ; chemical composition ; histology ; protein content ; glycogen ; lipid content ; energy metabolism ; water temperature ; seawater ; salinity ; chlorophyll ; mollusc control ; mariculture ; China
    Language English
    Dates of publication 2006-1124
    Size p. 752-759.
    Document type Article
    ZDB-ID 185380-6
    ISSN 0044-8486 ; 0044-8516
    ISSN 0044-8486 ; 0044-8516
    DOI 10.1016/j.aquaculture.2006.08.023
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 3275: Show issues

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  9. Book: Feng you lan zhe xue si xiang yan jiu

    Chen, Zhan'guo

    1999  

    Title variant Feng Youlan zhexue sixiang yanjiu
    Author's details Chen Zhan'guo zhu
    Language Chinese
    Size 2, 2, 8, 319 p, 21 cm
    Edition Di 1 ban
    Publisher Bei jing da xue chu ban she
    Publishing place Beijing
    Document type Book
    Note Includes bibliographical references
    ISBN 7301041675 ; 9787301041673
    Database Former special subject collection: coastal and deep sea fishing

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  10. Book ; Online ; E-Book: Dynamic neuroscience

    Chen, Zhe / Sarma, Sridevi V.

    statistics, modeling, and control

    2018  

    Author's details Zhe Chen, Sridevi V. Sarma editors
    Keywords Engineering ; Neurosciences ; Bioinformatics ; Neural networks (Computer science) ; Statistics ; Biomedical engineering
    Subject code 610.28
    Language English
    Size 1 Online-Ressource (XXI, 328 Seiten)
    Publisher Springer
    Publishing place Cham
    Publishing country Switzerland
    Document type Book ; Online ; E-Book
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT019585484
    ISBN 978-3-319-71976-4 ; 97833197119757 ; 3-319-71976-9
    DOI 10.1007/978-3-319-71976-4
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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