LIVIVO - Search results -

Search results

Result 1 - 10 of total 172

Search options

Article ; Online: Discovery of PL

Puhl, Ana C / Godoy, Andre S / Noske, Gabriela D / Nakamura, Aline M / Gawriljuk, Victor O / Fernandes, Rafaela S / Oliva, Glaucius / Ekins, Sean

ACS omega

2023 Volume 8, Issue 25, Page(s) 22603–22612

Abstract: There are very few small-molecule antivirals for SARS-CoV-2 that are either currently approved (or emergency authorized) in the US or globally, including remdesivir, molnupiravir, and paxlovid. The increasing number of SARS-CoV-2 variants that have ... ...

Abstract	There are very few small-molecule antivirals for SARS-CoV-2 that are either currently approved (or emergency authorized) in the US or globally, including remdesivir, molnupiravir, and paxlovid. The increasing number of SARS-CoV-2 variants that have appeared since the outbreak began over three years ago raises the need for continual development of updated vaccines and orally available antivirals in order to fully protect or treat the population. The viral main protease (M
Language	English
Publishing date	2023-06-14
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.3c01110
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: The protein disulfide isomerase inhibitor 3-methyltoxoflavin inhibits Chikungunya virus.

Puhl, Ana C / Fernandes, Rafaela S / Godoy, Andre S / Gil, Laura H V G / Oliva, Glaucius / Ekins, Sean

Bioorganic & medicinal chemistry

2023 Volume 83, Page(s) 117239

Abstract: Chikungunya virus (CHIKV) is the etiological agent of chikungunya fever, a (re)emerging arbovirus infection, that causes severe and often persistent arthritis, as well as representing a serious health concern worldwide for which no antivirals are ... ...

Abstract	Chikungunya virus (CHIKV) is the etiological agent of chikungunya fever, a (re)emerging arbovirus infection, that causes severe and often persistent arthritis, as well as representing a serious health concern worldwide for which no antivirals are currently available. Despite efforts over the last decade to identify and optimize new inhibitors or to reposition existing drugs, no compound has progressed to clinical trials for CHIKV and current prophylaxis is based on vector control, which has shown limited success in containing the virus. Our efforts to rectify this situation were initiated by screening 36 compounds using a replicon system and ultimately identified the natural product derivative 3-methyltoxoflavin with activity against CHIKV using a cell-based assay (EC
MeSH term(s)	Animals ; Humans ; Mice ; Antiviral Agents/chemistry ; Caco-2 Cells ; Chikungunya Fever/drug therapy ; Chikungunya virus/physiology ; Protein Disulfide-Isomerases/antagonists & inhibitors ; Virus Replication/drug effects ; Flavins/chemistry ; Flavins/pharmacology
Chemical Substances	Antiviral Agents ; Protein Disulfide-Isomerases (EC 5.3.4.1) ; Flavins
Language	English
Publishing date	2023-03-15
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	1161284-8
ISSN	1464-3391 ; 0968-0896
ISSN (online)	1464-3391
ISSN	0968-0896
DOI	10.1016/j.bmc.2023.117239
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3815: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Development of a Halochromic, Antimicrobial, and Antioxidant Starch-Based Film Containing Phenolic Extract from Jaboticaba Peel.

Luz, Rafaela F / Ferreira, Richard D R / Silva, Cassio N S / Miranda, Bruna M / Piccoli, Roberta H / Silva, Monique S / Paula, Ladyslene C / Leles, Maria Inês G / Fernandes, Kátia F / Cruz, Maurício V / Batista, Karla A

Foods (Basel, Switzerland)

2023 Volume 12, Issue 3

Abstract: In this study, the antioxidant, antimicrobial, mechanical, optical, and barrier attributes ... ...

Abstract	In this study, the antioxidant, antimicrobial, mechanical, optical, and barrier attributes of
Language	English
Publishing date	2023-02-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12030653
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages.

Ventura, Célia / Pinto, Fátima / Lourenço, Ana Filipa / Pedrosa, Jorge F S / Fernandes, Susete N / da Rosa, Rafaela R / Godinho, Maria Helena / Ferreira, Paulo J T / Louro, Henriqueta / Silva, Maria João

Bioengineering (Basel, Switzerland)

2023 Volume 10, Issue 8

Abstract: Cellulose micro/nanomaterials (CMNMs) are innovative materials with a wide spectrum of industrial and biomedical applications. Although cellulose has been recognized as a safe material, the unique properties of its nanosized forms have raised concerns ... ...

Abstract	Cellulose micro/nanomaterials (CMNMs) are innovative materials with a wide spectrum of industrial and biomedical applications. Although cellulose has been recognized as a safe material, the unique properties of its nanosized forms have raised concerns about their safety for human health. Genotoxicity is an endpoint that must be assessed to ensure that no carcinogenic risks are associated with exposure to nanomaterials. In this study, we evaluated the genotoxicity of two types of cellulose micro/nanofibrils (CMF and CNF) and one sample of cellulose nanocrystals (CNC), obtained from industrial bleached
Language	English
Publishing date	2023-08-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2746191-9
ISSN	2306-5354
ISSN	2306-5354
DOI	10.3390/bioengineering10080986
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: A Modified ELISA Method to Evaluate the Interaction of Schistosoma mansoni Proteins with Plasminogen.

Fernandes, Luis G V / Fernandes, Rafaela S / Nascimento, Ana L T O / Leite, Luciana C C

Methods in molecular biology (Clifton, N.J.)

2020 Volume 2151, Page(s) 185–195

Abstract: ... Kringle domains. To assess the activation of S. mansoni recombinant protein-bound PLG, the amidolytic activity ...

Abstract	An important aspect of host-pathogen interactions is the interference of secreted proteins with the fibrinolytic system. Herein, we describe a modified ELISA method used to evaluate the interaction of a recombinant Schistosoma mansoni protein with plasminogen (PLG). Using this protocol, we demonstrated that a secreted protein, recombinant venom allergen-like protein 18 (rSmVAL18) acts as a plasminogen receptor increasing its activation into plasmin in the presence of the urokinase-type plasminogen activator (uPA). PLG binding was determined by immobilizing human PLG in the plate and incubating with the recombinant protein; competitive binding with a lysine analog demonstrated the interaction of the protein lysine residues with PLG Kringle domains. To assess the activation of S. mansoni recombinant protein-bound PLG, the amidolytic activity of generated plasmin was measured using the D-Val-Leu-Lys 4-nitroanilide dihydrochloride substrate.
MeSH term(s)	Aminocaproic Acid/metabolism ; Animals ; Binding, Competitive ; Enzyme-Linked Immunosorbent Assay/methods ; Fibrinolysin/metabolism ; Helminth Proteins/metabolism ; Humans ; Plasminogen/metabolism ; Protein Binding ; Schistosoma mansoni/metabolism
Chemical Substances	Helminth Proteins ; Plasminogen (9001-91-6) ; Fibrinolysin (EC 3.4.21.7) ; Aminocaproic Acid (U6F3787206)
Language	English
Publishing date	2020-05-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-0635-3_15
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Therapeutic applications of snake venoms: An invaluable potential of new drug candidates.

Diniz-Sousa, Rafaela / Caldeira, Cleópatra A da S / Pereira, Soraya S / Da Silva, Saulo L / Fernandes, Pedro A / Teixeira, Luís M C / Zuliani, Juliana P / Soares, Andreimar M

International journal of biological macromolecules

2023 Volume 238, Page(s) 124357

Abstract: Animal venoms and their chemical compounds have aroused both empirical and scientific attention for ages. However, there has been a significant increase in scientific investigations in recent decades, allowing the production of various formulations that ... ...

Abstract	Animal venoms and their chemical compounds have aroused both empirical and scientific attention for ages. However, there has been a significant increase in scientific investigations in recent decades, allowing the production of various formulations that are helping in the development of many important tools for biotechnological, diagnostic, or therapeutic use, both in human and animal health, as well as in plants. Venoms are composed of biomolecules and inorganic compounds that may have physiological and pharmacological activities that are not related to their principal actions (prey immobilization, digestion, and defense). Snake venom toxins, mainly enzymatic and non-enzymatic proteins, and peptides have been identified as potential prototypes for new drugs and/or models for the development of pharmacologically active structural domains for the treatment of cancer, cardiovascular diseases, neurodegenerative and autoimmune diseases, pain, and infectious-parasitic diseases. This minireview aims to provide an overview of the biotechnological potential of animal venoms, with a focus on snakes, and to introduce the reader to the fascinating world of Applied Toxinology, where animal biodiversity can be used to develop therapeutic and diagnostic applications for humans.
MeSH term(s)	Animals ; Humans ; Snake Venoms/chemistry ; Snakes/metabolism ; Proteins/chemistry ; Peptides/pharmacology ; Neoplasms/drug therapy
Chemical Substances	Snake Venoms ; Proteins ; Peptides
Language	English
Publishing date	2023-04-05
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2023.124357
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 2374: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Analysis of selective fluorescence for the characterization of microplastic fibers: Use of a Nile Red-based analytical method to compare between natural and synthetic fibers.

Galvão, Luciana S / Ferreira, Rafaela R / Fernandes, Emília M S / Correia, Carla Almêda / Valera, Ticiane S / Dos Santos Rosa, Derval / Wiebeck, Hélio

Journal of hazardous materials

2022 Volume 443, Issue Pt A, Page(s) 130217

Abstract: The scientific community has been focusing on studying and understanding the extent of damage caused by microplastics (MPs) to flora, fauna, and humans, including the environmental and health risks associated with them. MPs with different morphologies ... ...

Abstract	The scientific community has been focusing on studying and understanding the extent of damage caused by microplastics (MPs) to flora, fauna, and humans, including the environmental and health risks associated with them. MPs with different morphologies have been described in different environments, with fibers being the most common type regardless of the environment. Various methods have been used to analyze MPs. Analytical methodologies such as visual inspection, spectroscopic methods, and others currently used to study MPs are time-consuming, and only subjective results are obtained when these methods are used for sample analysis. Researchers have used various dyes, such as Nile Red (NR), a selective fluorescent stain, to differentiate the polymers from the other sample components and address these problems. Using such dyes helps distinguish polymer particles from other contaminants present in the samples. We aimed to study the analytical process, morphology, and wettability of synthetic (such as polyethylene and polypropylene) and natural (such as linen and cotton) fibers using NR to characterize the fibers. The fibers were fragmented manually, and the samples were prepared using a cryomicrotome. The prepared samples were subjected to different NR incubation times of 30 min, 24 h, and 168 h, and characterized under ultraviolet light using optical microscopy. We investigated the effect of NR on different fibers, and the samples selection using the fluorescence properties generated when the fibers adsorbed the NR dye. The wettabilities of the samples indicated that polyethylene and polypropylene were hydrophobic, while linen and cotton were hydrophilic. Both synthetic and natural fibers exhibited fluorescence properties in the presence of NR. This increased the complexity of executing the MP characterization process, indicating that combined methodologies and optical and chemical identification processes should be used to characterize plastic specimens efficiently. We summarize and discuss the results and findings and provide recommendations for future laboratory research on microplastic fibers focusing on (I) microplastic selection, (II) stain preparation, and (III) microplastic characterization.
MeSH term(s)	Humans ; Microplastics ; Plastics/chemistry ; Polypropylenes ; Fluorescence ; Environmental Monitoring/methods ; Water Pollutants, Chemical/chemistry ; Polyethylene/analysis ; Coloring Agents
Chemical Substances	Microplastics ; Plastics ; nile red (P476F1L81G) ; Polypropylenes ; Water Pollutants, Chemical ; Polyethylene (9002-88-4) ; Coloring Agents
Language	English
Publishing date	2022-10-20
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1491302-1
ISSN	1873-3336 ; 0304-3894
ISSN (online)	1873-3336
ISSN	0304-3894
DOI	10.1016/j.jhazmat.2022.130217
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Therapeutic applications of snake venoms: An invaluable potential of new drug candidates

Diniz-Sousa, Rafaela / Caldeira, Cleópatra A. da S. / Pereira, Soraya S. / Da Silva, Saulo L. / Fernandes, Pedro A. / Teixeira, Luís M.C. / Zuliani, Juliana P. / Soares, Andreimar M.

International Journal of Biological Macromolecules. 2023 May, v. 238 p.124357-

2023

Abstract	Animal venoms and their chemical compounds have aroused both empirical and scientific attention for ages. However, there has been a significant increase in scientific investigations in recent decades, allowing the production of various formulations that are helping in the development of many important tools for biotechnological, diagnostic, or therapeutic use, both in human and animal health, as well as in plants. Venoms are composed of biomolecules and inorganic compounds that may have physiological and pharmacological activities that are not related to their principal actions (prey immobilization, digestion, and defense). Snake venom toxins, mainly enzymatic and non-enzymatic proteins, and peptides have been identified as potential prototypes for new drugs and/or models for the development of pharmacologically active structural domains for the treatment of cancer, cardiovascular diseases, neurodegenerative and autoimmune diseases, pain, and infectious-parasitic diseases. This minireview aims to provide an overview of the biotechnological potential of animal venoms, with a focus on snakes, and to introduce the reader to the fascinating world of Applied Toxinology, where animal biodiversity can be used to develop therapeutic and diagnostic applications for humans.
Keywords	biodiversity ; digestion ; drugs ; medicinal properties ; pain ; peptides ; snake venoms ; snakes ; therapeutics ; Therapeutic applications ; Venom ; Snake ; Toxins
Language	English
Dates of publication	2023-05
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2023.124357
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.B 2374: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: High-throughput Antiviral Assays to Screen for Inhibitors of Zika Virus Replication.

Fernandes, Rafaela S / Noske, Gabriela D / Gawriljuk, Victor O / de Oliveira, Ketllyn I Z / Godoy, Andre S / Mesquita, Nathalya C M R / Oliva, Glaucius

Journal of visualized experiments : JoVE

2021 , Issue 176

Abstract: Antiviral drug discovery requires the development of reliable biochemical and cellular assays that can be performed in high-throughput screening (HTS) formats. The flavivirus non-structural (NS) proteins are thought to co-translationally assemble on the ... ...

Abstract	Antiviral drug discovery requires the development of reliable biochemical and cellular assays that can be performed in high-throughput screening (HTS) formats. The flavivirus non-structural (NS) proteins are thought to co-translationally assemble on the endoplasmic reticulum (ER) membranes, forming the replication complex (RC). The NS3 and NS5 are the most studied enzymes of the RC and constitute the main targets for drug development due to their crucial roles in viral genome replication. NS3 protease domain, which requires NS2B as its cofactor, is responsible for the cleavage of the immature viral polyprotein into the mature NS proteins, whereas NS5 RdRp domain is responsible for the RNA replication. Herein, we describe in detail the protocols used in replicon-based screenings and enzymatic assays to test large compound libraries for inhibitors of the Zika virus (ZIKV) replication. Replicons are self-replicating subgenomic systems expressed in mammalian cells, in which the viral structural genes are replaced by a reporter gene. The inhibitory effects of compounds on viral RNA replication can be easily evaluated by measuring the reduction in the reporter protein activity. The replicon-based screenings were performed using a BHK-21 ZIKV replicon cell line expressing Renilla luciferase as a reporter gene. To characterize the specific targets of identified compounds, we established in-vitro fluorescence-based assays for recombinantly expressed NS3 protease and NS5 RdRp. The proteolytic activity of the viral protease was measured by using the fluorogenic peptide substrate Bz-nKRR-AMC, while the NS5 RdRp elongation activity was directly detected by the increase of the fluorescent signal of SYBR Green I during RNA elongation, using the synthetic biotinylated self-priming template 3'UTR-U30 (5'-biotin-U30-ACUGGAGAUCGAUCUCCAGU-3').
MeSH term(s)	Animals ; Antiviral Agents/metabolism ; High-Throughput Screening Assays ; Mammals ; Virus Replication ; Zika Virus/genetics ; Zika Virus Infection/drug therapy
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2021-10-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/62422
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Combinatorial delivery of doxorubicin and acridine orange by gold core silica shell nanospheres functionalized with poly(ethylene glycol) and 4-methoxybenzamide for cancer targeted therapy.

Guimarães, Rafaela S / Rodrigues, Carolina F / Fernandes, Natanael / de Melo-Diogo, Duarte / Ferreira, Paula / Correia, Ilídio J / Moreira, André F

Journal of inorganic biochemistry

2021 Volume 219, Page(s) 111433

Abstract: Combinatorial therapies based on the simultaneous administration of multiple drugs can lead to synergistic effects, increasing the efficacy of the cancer therapy. However, it is crucial to develop new delivery systems that can increase the drugs' ... ...

Abstract	Combinatorial therapies based on the simultaneous administration of multiple drugs can lead to synergistic effects, increasing the efficacy of the cancer therapy. However, it is crucial to develop new delivery systems that can increase the drugs' therapeutic selectivity and efficacy. Gold core silica shell (AuMSS) nanoparticles present physicochemical properties that allow their simultaneous application as drug delivery and imaging agents. Herein, poly(ethylene glycol) was modified with 4-methoxybenzamide and 3-(triethoxysilyl)propyl isocyanate (TPANIS) to create a novel surface functionalization capable of improving the colloidal stability and specificity of AuMSS nanospheres towards cancer cells. Moreover, a dual drug combination based on Doxorubicin (DOX) and Acridine orange (AO) was characterized and administered using the AuMSS-TPANIS nanospheres. The obtained results show that the DOX:AO drug combination can mediate a synergistic therapeutic effect in both HeLa and MCF-7 cells, particularly at the 2:1, 1:1, and 1:2 ratios. Additionally, the TPANIS functionalization increased the AuMSS nanospheres colloidal stability and selectivity towards MCF-7 cancer cells (overexpressing sigma receptors). Such also resulted in an enhanced cytotoxic effect against MCF-7 cells when administering the DOX:AO drug combination with the AuMSS-TPANIS nanospheres. Overall, the obtained results confirm the therapeutic potential of the DOX:AO drug combination as well as the targeting capacity of AuMSS-TPANIS, supporting its application in the cancer-targeted combinatorial chemotherapy.
MeSH term(s)	Acridine Orange/chemistry ; Acridine Orange/pharmacology ; Antineoplastic Combined Chemotherapy Protocols/chemistry ; Antineoplastic Combined Chemotherapy Protocols/pharmacology ; Benzamides/chemistry ; Cell Survival/drug effects ; Doxorubicin/chemistry ; Doxorubicin/pharmacology ; Drug Delivery Systems/methods ; Gold/chemistry ; HeLa Cells ; Humans ; MCF-7 Cells ; Nanospheres/chemistry ; Neoplasms/drug therapy ; Neoplasms/metabolism ; Polyethylene Glycols/chemistry ; Silicon Dioxide/chemistry
Chemical Substances	Benzamides ; Polyethylene Glycols (3WJQ0SDW1A) ; Gold (7440-57-5) ; Silicon Dioxide (7631-86-9) ; Doxorubicin (80168379AG) ; Acridine Orange (F30N4O6XVV)
Language	English
Publishing date	2021-03-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	162843-4
ISSN	1873-3344 ; 0162-0134
ISSN (online)	1873-3344
ISSN	0162-0134
DOI	10.1016/j.jinorgbio.2021.111433
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 1730: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito