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  1. Article ; Online: Membrane Trafficking Mechanisms and Their Biological Relevance.

    Akinwunmi, O Adeoye

    Archives of Razi Institute

    2023  Volume 78, Issue 5, Page(s) 1397–1412

    Abstract: Most chemicals expressed in mammalian cells have complex delivery and transport mechanisms to get to the right intracellular sites. One of these mechanisms transports most transmembrane proteins, as well as almost all secreted proteins, from the ... ...

    Abstract Most chemicals expressed in mammalian cells have complex delivery and transport mechanisms to get to the right intracellular sites. One of these mechanisms transports most transmembrane proteins, as well as almost all secreted proteins, from the endoplasmic reticulum, where they are formed, to their final location. Nearly all eukaryotic cells have a membrane trafficking mechanism that is both a prominent and critical component. This system, which consists of dynamically coupled compartments, supports the export and uptake of extracellular material, remodeling and signaling at the cellular interface, intracellular alignment, and maintenance of internal compartmentalization (organelles). In animal cells, this system enables both regular cellular activities and specialized tasks, such as neuronal transmission and hormone control. Human diseases, including neurodegenerative diseases, such as Alzheimer's disease, heart disease, and cancer, are associated with the dysfunction or dysregulation of the membrane trafficking system. Treatment and cure of human diseases depends on understanding the cellular and molecular principles underlying membrane trafficking pathways. A single gene mutation or mutations that result in impaired membrane trafficking cause a range of clinical disorders that are the result of changes in cellular homeostasis. Other eukaryotic organisms with significant economic and agricultural value, such as plants and fungi, also depend on the membrane trafficking system for their survival. In this review, we focused on the major human diseases associated with the process of membrane trafficking, providing a broad overview of membrane trafficking.
    MeSH term(s) Animals ; Humans ; Endoplasmic Reticulum/metabolism ; Neurodegenerative Diseases/metabolism ; Mammals
    Language English
    Publishing date 2023-10-31
    Publishing country Iran
    Document type Journal Article ; Review
    ZDB-ID 2555498-0
    ISSN 2008-9872 ; 0365-3439
    ISSN (online) 2008-9872
    ISSN 0365-3439
    DOI 10.22092/ARI.2023.78.5.1397
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Pharmacoinformatics studies of coenzyme Q10 and potassium polyacrylate on angiotensin-converting enzyme associated with hypertension.

    Adeoye, Akinwunmi O / Porta, Daniela J / Rivoira, María A / Garcia, Néstor H

    Journal of biomolecular structure & dynamics

    2023  , Page(s) 1–12

    Abstract: Coenzyme Q10's (CoQ10) favorable impact on cardiovascular diseases risk factors like hypertension and atherosclerosis is linked to the antioxidant action of CoQ10 in these conditions. This study showed the possible effects of CoQ10, potassium ... ...

    Abstract Coenzyme Q10's (CoQ10) favorable impact on cardiovascular diseases risk factors like hypertension and atherosclerosis is linked to the antioxidant action of CoQ10 in these conditions. This study showed the possible effects of CoQ10, potassium polyacrylate (PCK), and valsartan, a reference drug, on the angiotensin-converting enzyme (ACE), a crucial component of the renin-angiotensin system. The Glide tool on Maestro 11.1 was used to calculate the respective binding affinity and binding energy of these compounds towards ACE. The Schrödinger suite was used to run molecular dynamic simulations for 100 ns. The pkCSM tool was used to forecast the pharmacokinetic characteristics and toxicological effects. The SwissADME server was used to estimate the drug-like properties of these compounds. Based on their corresponding scoring values and the negative values of the binding free energies, molecular docking analysis of CoQ10 and PCK revealed that both exhibited favorable binding affinities towards the ACE, with CoQ10 having the highest binding scores. The results showed that both CoQ10 and PCK and the reference drug, valsartan, have some amino acids in common (at the pocket site of ACE) as the key residues for binding to ACE. Both CoQ10 and PCK demonstrated drug-like qualities and were not harmful, according to the predicted pharmacokinetics and toxicology studies. The results of this study suggest that because of its inhibitory interactions with ACE, CoQ10 in particular could be useful in regulating and reducing hypertension.Communicated by Ramaswamy H. Sarma.
    Language English
    Publishing date 2023-09-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 49157-3
    ISSN 1538-0254 ; 0739-1102
    ISSN (online) 1538-0254
    ISSN 0739-1102
    DOI 10.1080/07391102.2023.2254395
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  3. Article ; Online: Isolation, characterization and modulatory potentials of

    Sodeinde, Kehinde Oluseun / Adeoye, Akinwunmi Oluwaseun / Adesipo, Adedeji / Adeniyi, Adebayo A / Falode, John Adeolu / Obafemi, Tajudeen Olabisi / Olusanya, Samuel Olalekan / Twigge, Linette / Conradie, Jeanet / Mosaku, Timothy O

    Chinese herbal medicines

    2023  Volume 15, Issue 4, Page(s) 533–541

    Abstract: Objective: Secondary metabolites and polyphenolic compounds from medicinal plants have been demonstrated to have multiple biological functions with promising research and development prospects. This study examined the effect of : Methods: The ... ...

    Abstract Objective: Secondary metabolites and polyphenolic compounds from medicinal plants have been demonstrated to have multiple biological functions with promising research and development prospects. This study examined the effect of
    Methods: The compounds were isolated by vacuum liquid chromatography. Mitochondrial swelling was assessed as changes in absorbance under succinate-energized conditions.
    Results: 1
    Conclusion: Reduction in the activity of calcium ATPase and the expression of Caspase-3 and -9 were observed, suggesting that they could play a role in protecting physicochemical properties of membrane bilayers from free radical-induced severe cellular damage and be useful in the management of diseases where much apoptosis occurs.
    Language English
    Publishing date 2023-07-25
    Publishing country Singapore
    Document type Journal Article
    ISSN 2589-3610
    ISSN (online) 2589-3610
    DOI 10.1016/j.chmed.2023.01.006
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  4. Article ; Online: Modulatory Potential of Citrus sinensis and Moringa oleifera Extracts and Epiphytes on Rat Liver Mitochondrial Permeability Transition Pore.

    Adeoye, Akinwunmi O / Falode, John A / Jeje, Temitope O / Agbetuyi-Tayo, Praise T / Giwa, Sikirat M / Tijani, Yesirat O / Akinola, Damilola E

    Current drug discovery technologies

    2022  Volume 19, Issue 3, Page(s) e150322202238

    Abstract: Background: Bioactive agents from medicinal and dietary plants have been reported to modulate the mitochondrial membrane permeability transition pores.: Objective: This study investigated the in vitro effects of C. sinensis (CSE) and M. oleifera (MOE) ...

    Abstract Background: Bioactive agents from medicinal and dietary plants have been reported to modulate the mitochondrial membrane permeability transition pores.
    Objective: This study investigated the in vitro effects of C. sinensis (CSE) and M. oleifera (MOE) methanol leaf extracts and their epiphytes (CEP and MEP) on mitochondria permeability transition pores.
    Methods: In vitro antioxidant activities of the extracts were determined using standard procedures and quantification of polyphenolic compounds in the extract was done using HPLC-DAD. Opening of the mitochondrial permeability transition pores was assessed as mitochondrial swelling and observed spectrophotometrically as changes in absorbance under succinate-energized conditions. Cytochrome c release was also assessed spectrophotometrically.
    Results: From the results, CSE, MOE, CEP, and MEP inhibited lipid peroxidation and scavenged nitric oxide and DPPH radicals in a concentration-dependent manner. All extracts exhibited greater ferric reducing antioxidant potential. More so, the results showed that CSE, MOE, CEP, and MEP possess the substantive amount of total flavonoids and total phenolics. CSE and MOE had higher total flavonoids and total phenolic content when compared with the epiphytes. HPLC-DAD results revealed Tangeretin as the most abundant in CSE; Eriocitrin in citrus epiphytes; Moringine in MOE and Flavones in moringa epiphytes. All extracts inhibited calcium-induced opening of the pores in a concentration- dependent manner, with C. sinensis leaf extract (CSE) and moringa epiphyte (MEP) being the most potent in this regard with no significant release of cytochrome c at all concentrations.
    Conclusion: The results suggest that CSE and MEP have bioactive agents, which could be useful in the management of diseases where too much apoptosis occurs characterized by excessive tissue wastage, such as neurodegenerative conditions.
    MeSH term(s) Animals ; Antioxidants/pharmacology ; Citrus sinensis ; Cytochromes c/pharmacology ; Flavonoids/pharmacology ; Liver ; Mitochondrial Permeability Transition Pore ; Moringa oleifera/chemistry ; Phenols/pharmacology ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Plant Leaves ; Rats
    Chemical Substances Antioxidants ; Flavonoids ; Mitochondrial Permeability Transition Pore ; Phenols ; Plant Extracts ; Cytochromes c (9007-43-6)
    Language English
    Publishing date 2022-03-15
    Publishing country United Arab Emirates
    Document type Journal Article
    ISSN 1875-6220
    ISSN (online) 1875-6220
    DOI 10.2174/1570163819666220315124507
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Therapeutic potential of Ipomoea asarifolia on infected Swiss albino rats with Pseudomonas aeruginosa and Staphylococcus aureus

    Stephen Kayode S. Ojo / Gabriel Temitope Sunmonu / Akinwunmi O. Adeoye / Christiana Fisayo Akinwunmi / Moses Ifeoluwa Obakunle / Amos Olakunle Ojerinde / Oluwakemi J. Awakan

    Journal of HerbMed Pharmacology, Vol 11, Iss 3, Pp 409-

    2022  Volume 418

    Abstract: Introduction: Curative misuse of medicinal plants are worrisome with the paucity of histological information. This led to the investigation of Ipomoea asarifolia in Swiss albino rats infected with Pseudomonas aeruginosa and Staphylococcus aureus. Methods: ...

    Abstract Introduction: Curative misuse of medicinal plants are worrisome with the paucity of histological information. This led to the investigation of Ipomoea asarifolia in Swiss albino rats infected with Pseudomonas aeruginosa and Staphylococcus aureus. Methods: Extraction was done using the cold maceration method. The minimum inhibitory concentrations (MIC) of the extracts were determined using the micro-dilution method. Swiss albino rats of 6 sub-groups with 6 animals each (36 animals/organism) were administered with 0.3 ml single oral dose of P. aeruginosa and S. aureus respectively. The animals received treatment for 5 days as follows: 0.5 ml of 5% dimethyl sulphoxide (DMSO) (negative control), 250 mg/kg of amoxicillin (positive control), 2 mg/kg of whole plant extract, 4 mg/kg of whole plant extract, 2 mg/kg of leaf extract, and 4 mg/kg of leaf extract, respectively. The packed cell volume (PCV) and white blood count (WBC) of the animals were determined before and after treatment with histology examination of vital organs. Results: MIC for S. aureus was 2 mg/mL; the mortality in S. aureus group at 2 mg/kg was 66.7%. The PCV values (50.5±0.5, 45.0±1.0, and 50.5±1.5) decreased after infection, and a corresponding increase in the PCV was observed after treatment with the extracts. Also, a significant increase in the WBC values (3.40±0.35, 4.10±0.15, and 3.30±0.40) following infection and a corresponding decrease after treatment were observed. Congestion of vessels in the kidney was also observed. Conclusion: I. asarifolia has a dose-dependent antibacterial and curative activity, and could enhance innate immunity.
    Keywords medicinal plant ; gram negative bacteria ; gram positive bacteria ; antibacterial activity ; animal study ; histology ; Medicine (General) ; R5-920 ; Therapeutics. Pharmacology ; RM1-950
    Subject code 630
    Language English
    Publishing date 2022-07-01T00:00:00Z
    Publisher Shahrekord University of Medical Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Modulation of mitochondrial permeability transition pore opening by Myricetin and prediction of its-drug-like potential using

    Adeoye, Akinwunmi O / Falode, John A / Oladipupo, Olabimpe C / Obafemi, Tajudeen O / Oso, Babatunde J / Olaoye, Ige F

    Drug and chemical toxicology

    2022  Volume 46, Issue 5, Page(s) 1004–1014

    Abstract: Myricetin has been demonstrated to have multiple biological functions with promising research and development prospects. This study investigated the effect of myricetin on liver mitochondrial membrane permeability transition pores and its inhibitory ... ...

    Abstract Myricetin has been demonstrated to have multiple biological functions with promising research and development prospects. This study investigated the effect of myricetin on liver mitochondrial membrane permeability transition pores and its inhibitory potential on proteins that are important in the apoptotic process in silico. Mitochondrial swelling was assessed as changes in absorbance under succinate-energized conditions. Cytochrome c release, mitochondrial-lipid peroxidation, caspase 3 and 9 expressions, as well as calcium ATPase, were assessed. Pharmacokinetic properties of myricetin were predicted through the SwissADME server while the binding affinity of myricetin toward the proteins was computed using the AutodockVina Screening tool. The conformational stability of protein-ligand interactions was evaluated using molecular dynamics simulations analysis through the iMODS server. Myricetin inhibited the opening of the mitochondrial permeability transition pore and also reversed the increase in mitochondrial lipid peroxidation caused by calcium and other toxicants. Myricetin also caused a reduction in the expression of caspase 3 and 9 as well as calcium ATPase activity. The molecular docking results revealed that myricetin had a considerable binding affinity to the pocket site of caspase 3 and 9 as well as calcium ATPase. Myricetin showed a good drug-likeness based on the predicted pharmacokinetic properties as revealed by low CYP 450 inhibitory promiscuity and relatively low toxicity. It could therefore be suggested that myricetin could be useful in the management of diseases where too many apoptosis occur characterized by excessive tissue wastage such as neurodegenerative conditions and could as well play a role in protecting the physicochemical properties of membrane bilayers from free radical-induced severe cellular damage.
    MeSH term(s) Rats ; Animals ; Mitochondrial Permeability Transition Pore/metabolism ; Caspase 3/metabolism ; Mitochondrial Membrane Transport Proteins/metabolism ; Mitochondrial Membrane Transport Proteins/pharmacology ; Rats, Wistar ; Mitochondria, Liver ; Molecular Docking Simulation ; Apoptosis ; Calcium/metabolism
    Chemical Substances Mitochondrial Permeability Transition Pore ; myricetin (76XC01FTOJ) ; Caspase 3 (EC 3.4.22.-) ; Mitochondrial Membrane Transport Proteins ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2022-08-29
    Publishing country United States
    Document type Journal Article
    ZDB-ID 548368-2
    ISSN 1525-6014 ; 0148-0545
    ISSN (online) 1525-6014
    ISSN 0148-0545
    DOI 10.1080/01480545.2022.2117372
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: An intensive follow-up in subjects with cardiometabolic high-risk.

    Pérez, Hernán A / Adeoye, Akinwunmi O / Aballay, Laura / Armando, Luis A / García, Néstor H

    Nutrition, metabolism, and cardiovascular diseases : NMCD

    2021  Volume 31, Issue 10, Page(s) 2860–2869

    Abstract: Background and aim: Addressing chronic problems requires a model of care that promotes self-management of the disease and facilitates adherence to treatment. This project was designed to enhance patient's clinical and functional outcomes through a ... ...

    Abstract Background and aim: Addressing chronic problems requires a model of care that promotes self-management of the disease and facilitates adherence to treatment. This project was designed to enhance patient's clinical and functional outcomes through a Comprehensive Model to be implemented in our health system and to evaluate the results.
    Methods and results: Different population stratification tools were tested and designed to classify subjects according to different variables. We have developed a program to detect and screen cardiometabolic risk by integrating most of the Chronic Care Model recommendations through in-house developed management software (MoviHealth®). From the results, 1317 subjects were evaluated (27% of the whole population) during the first year of follow-up which significantly improved for all variables along the follow-up period. The blood pressure of the hypertensive population in 2010 and 2015 showed the importance of enrollment of subjects and the optimization of the blood pressure control. The result of HbA1c observed in 2010 decreased progressively to 7.1 ± 1.4% in 2015, and dyslipidemia levels improved gradually. The number of cardiovascular events requiring hospitalization decreased significantly (48%), from 1.9 events per 100 subjects in 2011 to 0.98 in 2015.
    Conclusion: Our program has combined strategies for the prevention and control of non-communicable diseases, incorporating interventions to control risk factors and to reduce morbidity and mortality. It also had improvements in life quality, accessibility to health-care services, and the promotion of self-care.
    MeSH term(s) Adult ; Aged ; Aged, 80 and over ; Argentina/epidemiology ; Biomarkers/blood ; Blood Glucose/metabolism ; Blood Pressure ; Cardiometabolic Risk Factors ; Cardiovascular Diseases/diagnosis ; Cardiovascular Diseases/epidemiology ; Cardiovascular Diseases/prevention & control ; Diabetes Mellitus/diagnosis ; Diabetes Mellitus/epidemiology ; Diabetes Mellitus/therapy ; Dyslipidemias/diagnosis ; Dyslipidemias/epidemiology ; Dyslipidemias/therapy ; Female ; Follow-Up Studies ; Glycated Hemoglobin A/metabolism ; Health Status ; Humans ; Hypertension/diagnosis ; Hypertension/epidemiology ; Hypertension/therapy ; Lipids/blood ; Male ; Metabolic Syndrome/diagnosis ; Metabolic Syndrome/epidemiology ; Metabolic Syndrome/therapy ; Middle Aged ; Preventive Health Services ; Prognosis ; Program Evaluation ; Protective Factors ; Quality of Life ; Risk Assessment ; Time Factors ; Young Adult
    Chemical Substances Biomarkers ; Blood Glucose ; Glycated Hemoglobin A ; Lipids ; hemoglobin A1c protein, human
    Language English
    Publishing date 2021-06-25
    Publishing country Netherlands
    Document type Journal Article ; Observational Study ; Research Support, Non-U.S. Gov't
    ZDB-ID 1067704-5
    ISSN 1590-3729 ; 0939-4753
    ISSN (online) 1590-3729
    ISSN 0939-4753
    DOI 10.1016/j.numecd.2021.06.011
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  8. Article: An intensive follow-up in subjects with cardiometabolic high-risk

    Pérez, Hernán A. / Adeoye, Akinwunmi O. / Aballay, Laura / Armando, Luis A. / García, Néstor H.

    The Italian Diabetes Society, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition and the Department of Clinical Medicine and Surgery, Federico II University Nutrition, metabolism, and cardiovascular diseases. 2021 Sept. 22, v. 31, no. 10

    2021  

    Abstract: Addressing chronic problems requires a model of care that promotes self-management of the disease and facilitates adherence to treatment. This project was designed to enhance patient's clinical and functional outcomes through a Comprehensive Model to be ... ...

    Abstract Addressing chronic problems requires a model of care that promotes self-management of the disease and facilitates adherence to treatment. This project was designed to enhance patient's clinical and functional outcomes through a Comprehensive Model to be implemented in our health system and to evaluate the results.Different population stratification tools were tested and designed to classify subjects according to different variables. We have developed a program to detect and screen cardiometabolic risk by integrating most of the Chronic Care Model recommendations through in-house developed management software (MoviHealth®). From the results, 1317 subjects were evaluated (27% of the whole population) during the first year of follow-up which significantly improved for all variables along the follow-up period. The blood pressure of the hypertensive population in 2010 and 2015 showed the importance of enrollment of subjects and the optimization of the blood pressure control. The result of HbA1c observed in 2010 decreased progressively to 7.1 ± 1.4% in 2015, and dyslipidemia levels improved gradually. The number of cardiovascular events requiring hospitalization decreased significantly (48%), from 1.9 events per 100 subjects in 2011 to 0.98 in 2015.Our program has combined strategies for the prevention and control of non-communicable diseases, incorporating interventions to control risk factors and to reduce morbidity and mortality. It also had improvements in life quality, accessibility to health-care services, and the promotion of self-care.
    Keywords blood pressure ; computer software ; health services ; hyperlipidemia ; metabolism ; models ; morbidity ; mortality ; nutrition ; patients ; quality of life
    Language English
    Dates of publication 2021-0922
    Size p. 2860-2869.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1067704-5
    ISSN 0939-4753
    ISSN 0939-4753
    DOI 10.1016/j.numecd.2021.06.011
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Repurposing of chloroquine and some clinically approved antiviral drugs as effective therapeutics to prevent cellular entry and replication of coronavirus.

    Adeoye, Akinwunmi O / Oso, Babatunde Joseph / Olaoye, Ige Francis / Tijjani, Habibu / Adebayo, Ahmed I

    Journal of biomolecular structure & dynamics

    2020  Volume 39, Issue 10, Page(s) 3469–3479

    Abstract: The reemergence of coronavirus prompts the need for the development of effective therapeutics to prevent the cellular entry and replication of coronavirus. This study demonstrated the putative inhibitory potential of lopinavir, remdesivir, oseltamir, ... ...

    Abstract The reemergence of coronavirus prompts the need for the development of effective therapeutics to prevent the cellular entry and replication of coronavirus. This study demonstrated the putative inhibitory potential of lopinavir, remdesivir, oseltamir, azithromycin, ribavirin, and chloroquine towards V-ATPase, protein kinase A, SARS-CoV spike glycoprotein/ACE-2 complex and viral proteases. The pharmacodynamic and pharmacokinetic properties were predicted through the pkCSM server while the corresponding binding affinity of the selected drugs towards the proteins was computed using AutodockVina Screening tool. The ADMET properties revealed all the drugs possess drug-like properties. Lopinavir has the highest binding affinities to the pocket site of SARS-CoV spike glycoprotein/ACE-2 complex, cyclic AMP-dependent protein kinase A and 3-Chymotrypsin like protease while redemsivir has the highest binding affinities for vacuolar proton-translocating ATPase (V-ATPase) and papain-like proteins. The amino acids Asp269, Leu370, His374, and His345 were predicted as the key residues for lopinavir binding to human SARS-CoV spike glycoprotein/ACE-2 complex while His378, Tyr515, Leu73, Leu100, Phe32 and Phe40 for remdesivir and Tyr510, Phe504, Met62, Tyr50, and His378 were predicted for azithromycin as the key residues for binding to SARS-CoV spike glycoprotein/ACE-2 complex. Moreover, it was also observed that chloroquine has appreciable binding affinities for 3-Chymotrpsin- like protease and cyclic AMP-dependent protein kinase A when compared to Oseltamivir and ribavirin. The study provided evidence suggesting putative repurposing of the selected drugs for the development of valuable drugs for the prevention of cellular entry and replication of coronavirus.Communicated by Ramaswamy H. Sarma.
    MeSH term(s) Adenosine Monophosphate/analogs & derivatives ; Adenosine Monophosphate/pharmacology ; Alanine/analogs & derivatives ; Alanine/pharmacology ; Antiviral Agents/pharmacology ; Azithromycin/pharmacology ; Chloroquine/pharmacology ; Drug Repositioning ; Humans ; Lopinavir/pharmacology ; Molecular Docking Simulation ; SARS Virus/drug effects ; SARS Virus/physiology ; Virus Internalization/drug effects ; Virus Replication/drug effects
    Chemical Substances Antiviral Agents ; Lopinavir (2494G1JF75) ; remdesivir (3QKI37EEHE) ; Adenosine Monophosphate (415SHH325A) ; Azithromycin (83905-01-5) ; Chloroquine (886U3H6UFF) ; Alanine (OF5P57N2ZX)
    Keywords covid19
    Language English
    Publishing date 2020-05-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 49157-3
    ISSN 1538-0254 ; 0739-1102
    ISSN (online) 1538-0254
    ISSN 0739-1102
    DOI 10.1080/07391102.2020.1765876
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Inhibition of liver mitochondrial membrane permeability transition pore opening by quercetin and vitamin E in streptozotocin-induced diabetic rats.

    Daniel, Oluwatoyin O / Adeoye, Akinwunmi O / Ojowu, John / Olorunsogo, Olufunso O

    Biochemical and biophysical research communications

    2018  Volume 504, Issue 2, Page(s) 460–469

    Abstract: Diabetes mellitus is a chronic metabolic disorder characterized by rise in blood glucose levels and generation of free radicals which could induce mitochondrial membrane permeability transition (MMPT) pore opening. This study examined the in vivo action ... ...

    Abstract Diabetes mellitus is a chronic metabolic disorder characterized by rise in blood glucose levels and generation of free radicals which could induce mitochondrial membrane permeability transition (MMPT) pore opening. This study examined the in vivo action of quercetin and vitamin E on MMPT in the liver of streptozotocin-induced diabetic rats orally pre-treated with 30 mg quercetin/kg body weight (STZQ), 10 mg vitamin E/kg body weight (STZVit.E) and 0.6 mg glibenclamide/kg body weight (STZG). Male albino wistar rats were used in the study and were injected intraperitonially with streptozotocin (STZ) (45 mg/kg body weight) in citrate buffer. The degrees of serum and tissue peroxidation, alanine and aspartate aminotransferase activity were investigated. MMPT pore was assessed as mitochondrial swelling and was monitored spectrophotometrically as changes in absorbance at 540 nm under succinate-energized condition. There was significant increase in serum glucose level, degree of tissue peroxidation, alanine and aspartate aminotransferase activity, cholesterol and triglycerides values in the diabetic control rats following streptozotocin induction. All the treatment had effect on the damage caused by streptozotocin. Quercetin exhibited the highest chemopreventive activity. Quercetin and vitamin E significantly reduced the blood glucose level, degree of tissue peroxidation and alanine and aspartate amino transferase activity. In vivo rat liver MMPT pore was opened in diabetic control rats and significantly inhibited in STZQ, STZV and STZG treated groups by 72.3%, 58.5% and 87.5% respectively. The activity of quercetin and vitamin E were substantiated by histopathological evaluation. Our study suggests that quercetin is an effective therapeutic agent for preventing hepatic tissues from oxidative stress resulting from streptozotocin-induced diabetes by inhibiting apoptotic processes in their target cells.
    MeSH term(s) Administration, Oral ; Animals ; Antioxidants/pharmacology ; Apoptosis/drug effects ; Diabetes Mellitus, Experimental/chemically induced ; Diabetes Mellitus, Experimental/metabolism ; Glucose/chemistry ; Glyburide/administration & dosage ; Lipid Peroxidation ; Liver/drug effects ; Male ; Mitochondria, Liver/drug effects ; Mitochondria, Liver/metabolism ; Mitochondrial Membrane Transport Proteins/drug effects ; Mitochondrial Membrane Transport Proteins/metabolism ; Mitochondrial Membranes/drug effects ; Mitochondrial Membranes/metabolism ; Mitochondrial Swelling/drug effects ; Oxidative Stress ; Permeability ; Quercetin/pharmacology ; Rats ; Rats, Wistar ; Streptozocin ; Time Factors ; Vitamin E/chemistry ; Vitamin E/pharmacology
    Chemical Substances Antioxidants ; Mitochondrial Membrane Transport Proteins ; mitochondrial permeability transition pore ; Vitamin E (1406-18-4) ; Streptozocin (5W494URQ81) ; Quercetin (9IKM0I5T1E) ; Glucose (IY9XDZ35W2) ; Glyburide (SX6K58TVWC)
    Language English
    Publishing date 2018-09-06
    Publishing country United States
    Document type Journal Article
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2018.08.114
    Database MEDical Literature Analysis and Retrieval System OnLINE

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