Article ; Online: Solid-phase extraction of ursolic acid from herb using beta-cyclodextrin-based molecularly imprinted microspheres.
2008 Volume 31, Issue 20, Page(s) 3573–3580
Abstract: A new method was employed to solid-phase extract ursolic acid from Ilex kudingcha C. J. Tseng using molecularly imprinted microspheres (MIMs) as the sorbent. Using a surface molecular imprinting technique, MIMs for ursolic acid were prepared with bonded ... ...
| Abstract | A new method was employed to solid-phase extract ursolic acid from Ilex kudingcha C. J. Tseng using molecularly imprinted microspheres (MIMs) as the sorbent. Using a surface molecular imprinting technique, MIMs for ursolic acid were prepared with bonded beta-CD and acrylamide in combination based on functionalized poly(glycidyl methacrylate) microspheres (F-PGMA). Compared with non-MIMs (NIMs), MIMs showed high adsorption capacity, significant selectivity, and good site accessibility for ursolic acid. The maximum static adsorption capacities of the MIMs and NIMs for ursolic acid were 42.5 and 4.9 micromol/g, respectively. Chromatographic analysis shows that ursolic acid and oleanolic acid could be separated well when MIMs were used as the stationary phase of HPLC. The conditions of molecularly imprinted SPE (MISPE) of ursolic acid from the herb extract were optimized using different concentrations of ethanol solutions as loading, washing, and eluting solutions. The successful extraction of ursolic acid by MIMs provided a possible innovative approach to separate ursolic acid from herb. |
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| MeSH term(s) | Adsorption ; Antineoplastic Agents, Phytogenic/chemistry ; Antineoplastic Agents, Phytogenic/isolation & purification ; Chromatography, High Pressure Liquid/methods ; Hydrogen-Ion Concentration ; Ilex/chemistry ; Microspheres ; Molecular Imprinting ; Molecular Structure ; Oleanolic Acid/chemistry ; Oleanolic Acid/isolation & purification ; Solid Phase Extraction/instrumentation ; Solid Phase Extraction/methods ; Triterpenes/chemistry ; Triterpenes/isolation & purification ; beta-Cyclodextrins/chemistry ; Ursolic Acid |
| Chemical Substances | Antineoplastic Agents, Phytogenic ; Triterpenes ; beta-Cyclodextrins ; Oleanolic Acid (6SMK8R7TGJ) ; betadex (JV039JZZ3A) |
| Language | English |
| Publishing date | 2008-10-08 |
| Publishing country | Germany |
| Document type | Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2047990-6 |
| ISSN | 1615-9314 ; 1615-9306 |
| ISSN (online) | 1615-9314 |
| ISSN | 1615-9306 |
| DOI | 10.1002/jssc.200800362 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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