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  1. Article ; Online: Navigating between perpendicular drop and tangent skimming methods for M-protein quantification: a call for clarification of guidelines.

    Cabo, Julien / Favresse, Julien

    Clinical chemistry and laboratory medicine

    2023  Volume 62, Issue 3, Page(s) e74–e77

    Language English
    Publishing date 2023-10-02
    Publishing country Germany
    Document type Letter
    ZDB-ID 1418007-8
    ISSN 1437-4331 ; 1434-6621 ; 1437-8523
    ISSN (online) 1437-4331
    ISSN 1434-6621 ; 1437-8523
    DOI 10.1515/cclm-2023-0730
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  2. Article ; Online: Corrigendum to "Application of analytical performance specifications for urine test strip methods: Importance of reflectance signals" [Clin. Chim. Acta 550 (2023) 117534].

    Cabo, Julien / Favresse, Julien

    Clinica chimica acta; international journal of clinical chemistry

    2023  Volume 552, Page(s) 117670

    Language English
    Publishing date 2023-12-12
    Publishing country Netherlands
    Document type Published Erratum
    ZDB-ID 80228-1
    ISSN 1873-3492 ; 0009-8981
    ISSN (online) 1873-3492
    ISSN 0009-8981
    DOI 10.1016/j.cca.2023.117670
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  3. Article ; Online: Application of analytical performance specifications for urine test strip methods: Importance of reflectance signals.

    Cabo, Julien / Favresse, Julien

    Clinica chimica acta; international journal of clinical chemistry

    2023  Volume 550, Page(s) 117534

    Abstract: Introduction: Urinalysis is essential for diagnosing kidney-related medical conditions. Urine test strip analysis serves as an initial and efficient screening method for reflex testing with accurate quantitative methods.: Materials and methods: ... ...

    Abstract Introduction: Urinalysis is essential for diagnosing kidney-related medical conditions. Urine test strip analysis serves as an initial and efficient screening method for reflex testing with accurate quantitative methods.
    Materials and methods: Freshly voided urines (n = 206) were analysed using two urine test strip brands on UC-MAX (Menarini) and cobas u 601 (Roche Diagnostics) instruments. Ordinal scale categories and reflectance signals (if available) were both used for the comparison with reference quantitative methods for glucose, proteins and albumin (cobas 503). Samples were considered positive when glucose > 15 or ≥ 54 mg/dL, proteins ≥ 200 mg/L and albumin ≥ 10 mg/L. Optimized reflectance thresholds were calculated by ROC curve analysis. Analytical performance specifications (APS) for trueness of test strip were gathered from the EFLM guideline (FP
    Results: Reflectance signals were significantly lower in urine samples considered positive by the reference method (p < 0.0001). Reflectance signals were also correlated with quantitative measurements, showing strong correlation (0.754 to 0.969). Only the use of optimized reflectance thresholds on cobas u 601 achieved at least the minimum EFLM APS (FP
    Conclusion: The use of reflectance signals from urine test strips enhanced accuracy for glucose, proteins, and albumin measurement and may contribute to improve diagnosis of diverse kidney-related conditions.
    MeSH term(s) Humans ; Reagent Strips ; Urinalysis/methods ; Glucose/analysis ; Proteins ; Albumins
    Chemical Substances Reagent Strips ; Glucose (IY9XDZ35W2) ; Proteins ; Albumins
    Language English
    Publishing date 2023-09-20
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 80228-1
    ISSN 1873-3492 ; 0009-8981
    ISSN (online) 1873-3492
    ISSN 0009-8981
    DOI 10.1016/j.cca.2023.117534
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  4. Article ; Online: Cellular immunity against SARS-CoV-2 is predominantly boosted in vaccinated individuals with no history of infection.

    Favresse, Julien / Cabo, Julien / Douxfils, Jonathan

    The Journal of infection

    2023  Volume 87, Issue 2, Page(s) e31–e32

    MeSH term(s) Humans ; SARS-CoV-2 ; COVID-19 ; Immunity, Cellular ; Antibodies, Viral ; Vaccination
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2023-05-19
    Publishing country England
    Document type Letter ; Comment
    ZDB-ID 424417-5
    ISSN 1532-2742 ; 0163-4453
    ISSN (online) 1532-2742
    ISSN 0163-4453
    DOI 10.1016/j.jinf.2023.05.014
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  5. Article ; Online: Neutralizing antibody response to XBB.1.5, BA.2.86, FL.1.5.1 and JN.1 six months after the BNT162b2 bivalent booster.

    Favresse, Julien / Gillot, Constant / Cabo, Julien / David, Clara / Dogné, Jean-Michel / Douxfils, Jonathan

    International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

    2024  , Page(s) 107028

    Abstract: Introduction: An increase evasion of the SARS-CoV-2 virus towards vaccination strategies and natural immunity has been rapidly described notably due to mutations in the spike receptor binding domain and the N-terminal domain.: Material and methods: ... ...

    Abstract Introduction: An increase evasion of the SARS-CoV-2 virus towards vaccination strategies and natural immunity has been rapidly described notably due to mutations in the spike receptor binding domain and the N-terminal domain.
    Material and methods: Participants of the CRO-VAX HCP study who received the bivalent booster were followed at 6 months. A pseudovirus-neutralization test was used to assess the neutralization potency of antibodies against D614G, Delta, BA.1, BA.5, XBB.1.5, BA.2.86, FL.1.5.1, and JN-1.
    Results: The neutralizing capacity of antibodies against Omicron variant or subvariants was significantly reduced compared to D614G and Delta (p<0.0001). The lowest neutralizing response that was observed with JN-1 (GMT=22.1) was also significantly lower compared to XBB.1.5 (GMT=29.5, p<0.0001), BA.2.86 (GMT=29.6, p<0.0001), and FL.1.5.1 (GMT=25.2, p<0.0001). Participants that contracted a breakthrough infection due to XBB.1.5 had significantly higher neutralizing antibodies against all variants compared to uninfected participants, especially against Omicron variant and subvariants.
    Conclusion: Our results confirm that JN.1 is one of the most immune evading variants to date and that the BA.2.86 subvariant did not show an increased immunity escape compared to XBB.1.5. The stronger response in BKI with Omicron variant and subvariants supports the need to use vaccine antigens that target circulating variants.
    Language English
    Publishing date 2024-04-05
    Publishing country Canada
    Document type Journal Article
    ZDB-ID 1331197-9
    ISSN 1878-3511 ; 1201-9712
    ISSN (online) 1878-3511
    ISSN 1201-9712
    DOI 10.1016/j.ijid.2024.107028
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  6. Article ; Online: Evaluation of four automated clinical analyzers for the determination of total 25(OH)D in comparison to a certified LC-MS/MS.

    Favresse, Julien / Fangazio, Marco / Cotton, Frédéric / Wolff, Fleur

    Clinical chemistry and laboratory medicine

    2023  Volume 61, Issue 8, Page(s) 1420–1427

    Abstract: Objectives: The aim of this study was to compare the results of five methods for the determination of total 25(OH)D. For that purpose, two mass spectrometry and three immunoassay methods were used.: Methods: A total of 124 serum samples were analyzed ...

    Abstract Objectives: The aim of this study was to compare the results of five methods for the determination of total 25(OH)D. For that purpose, two mass spectrometry and three immunoassay methods were used.
    Methods: A total of 124 serum samples were analyzed on five different methods (i.e., a reference LC-MS/MS, Cascadion, Lumipulse, Roche Elecsys II and Roche Elecsys III). Analytical performance against LC-MS/MS was evaluated and compared to the Milan models 1 (analytical performance based on the clinical outcome using thresholds of 12, 20 and 30 ng/mL) and 2 (analytical performance based on biological variation). Additionally, imprecision studies and accuracy using NIST SRM972a samples were carried out.
    Results: Compared to the reference LC-MS/MS method, the Lumipulse and the Roche Elecsys III assays reached the optimal criterion for bias, while the Cascadion met the desirable one. The Roche Elecsys II was not able to reach the minimal criteria. The proportion of correctly classified patients was higher using the Cascadion (95.2%) compared to the three immunoassays. In addition to its better precision, the Cascadion was not impacted by a high concentration of 3-epi-25(OH)D
    Conclusions: Compared to the LC-MS/MS reference method, the Cascadion presented the highest level of concordance at medical decision cut-offs for total 25(OH)D and reached the desirable specification for bias. Moreover, the presence of 3-epi-25(OH)D
    MeSH term(s) Humans ; Chromatography, Liquid/methods ; Tandem Mass Spectrometry/methods ; Vitamin D ; Immunoassay/methods
    Chemical Substances 25-hydroxyvitamin D (A288AR3C9H) ; Vitamin D (1406-16-2)
    Language English
    Publishing date 2023-02-15
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1418007-8
    ISSN 1437-4331 ; 1434-6621 ; 1437-8523
    ISSN (online) 1437-4331
    ISSN 1434-6621 ; 1437-8523
    DOI 10.1515/cclm-2022-1129
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  7. Article ; Online: Biotin interference in immunoassays: water under the bridge?

    Wauthier, Loris / Cabo, Julien / Eucher, Christine / Rosseels, Catherine / Elsen, Marc / Favresse, Julien

    Clinical chemistry and laboratory medicine

    2023  Volume 61, Issue 10, Page(s) e196–e199

    MeSH term(s) Humans ; Biotin ; Streptavidin ; Immunoassay
    Chemical Substances Biotin (6SO6U10H04) ; Streptavidin (9013-20-1)
    Language English
    Publishing date 2023-04-11
    Publishing country Germany
    Document type Letter
    ZDB-ID 1418007-8
    ISSN 1437-4331 ; 1434-6621 ; 1437-8523
    ISSN (online) 1437-4331
    ISSN 1434-6621 ; 1437-8523
    DOI 10.1515/cclm-2023-0242
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  8. Article ; Online: Evaluations of SARS-CoV-2 Serological Assay Performance Need Inclusion of Long-Term Samples.

    Favresse, Julien / Douxfils, Jonathan

    Journal of clinical microbiology

    2021  Volume 59, Issue 6

    MeSH term(s) Antibodies, Viral ; COVID-19 ; Humans ; SARS-CoV-2 ; Sensitivity and Specificity ; Spike Glycoprotein, Coronavirus
    Chemical Substances Antibodies, Viral ; Spike Glycoprotein, Coronavirus
    Language English
    Publishing date 2021-05-19
    Publishing country United States
    Document type Letter
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/JCM.00487-21
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  9. Article ; Online: Importance of sample dilution in the evaluation of the antibody response after SARS-CoV-2 vaccination.

    Favresse, Julien / Douxfils, Jonathan

    The Journal of infection

    2021  Volume 84, Issue 1, Page(s) 94–118

    MeSH term(s) Antibodies, Viral ; Antibody Formation ; COVID-19 ; COVID-19 Vaccines ; Humans ; SARS-CoV-2 ; Vaccination
    Chemical Substances Antibodies, Viral ; COVID-19 Vaccines
    Language English
    Publishing date 2021-07-04
    Publishing country England
    Document type Letter ; Comment
    ZDB-ID 424417-5
    ISSN 1532-2742 ; 0163-4453
    ISSN (online) 1532-2742
    ISSN 0163-4453
    DOI 10.1016/j.jinf.2021.07.001
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  10. Article ; Online: Addressing standardized definitions of post-COVID and long-COVID.

    Lippi, Giuseppe / Henry, Brandon M / Favresse, Julien / Plebani, Mario

    Clinical chemistry and laboratory medicine

    2023  Volume 61, Issue 8, Page(s) 1361–1362

    MeSH term(s) Humans ; Post-Acute COVID-19 Syndrome ; COVID-19
    Language English
    Publishing date 2023-04-25
    Publishing country Germany
    Document type Editorial
    ZDB-ID 1418007-8
    ISSN 1437-4331 ; 1434-6621 ; 1437-8523
    ISSN (online) 1437-4331
    ISSN 1434-6621 ; 1437-8523
    DOI 10.1515/cclm-2023-0390
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