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  1. Article: Improved viability of probiotics encapsulated in soybean protein isolate matrix microcapsules by coacervation and cross-linking modification

    Hu, Ronghai / Dong, Dejun / Hu, Jielun / Liu, Huan

    Food hydrocolloids

    2023  Volume 138, Issue -, Page(s) 108457

    Language English
    Document type Article
    ZDB-ID 742742-6
    ISSN 0268-005X
    Database Current Contents Nutrition, Environment, Agriculture

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    In stock of ZB MED Bonn / Germany

    Z 4414: Show issues

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  2. Article: Improved Loading Capacity and Viability of Probiotics Encapsulated in Alginate Hydrogel Beads by In Situ Cultivation Method.

    Huang, Yachun / Zhang, Lin / Hu, Jielun / Liu, Huan

    Foods (Basel, Switzerland)

    2023  Volume 12, Issue 11

    Abstract: The objective of this research was to encapsulate probiotics by alginate hydrogel beads based on an in situ cultivation method and investigate the influences on the cell loading capacity, surface and internal structure of hydrogel beads and in vitro ... ...

    Abstract The objective of this research was to encapsulate probiotics by alginate hydrogel beads based on an in situ cultivation method and investigate the influences on the cell loading capacity, surface and internal structure of hydrogel beads and in vitro gastrointestinal digestion property of cells. Hydrogel beads were prepared by extrusion and cultured in MRS broth to allow probiotics to grow inside. Up to 10.34 ± 0.02 Log CFU/g of viable cell concentration was obtained after 24 h of in situ cultivation, which broke through the bottleneck of low viable cell counts in the traditional extrusion method. Morphology and rheological analyses showed that the structure of the eventually formed probiotic hydrogel beads can be loosed by the existence of hydrogen bond interaction with water molecules and the internal growth of probiotic microcolonies, while it can be tightened by the acids metabolized by the probiotic bacteria during cultivation. In vitro gastrointestinal digestion analysis showed that great improvement with only 1.09 Log CFU/g of loss in viable cells was found after the entire 6 h of digestion. In conclusion, the current study demonstrated that probiotic microcapsules fabricated by in situ cultivation method have the advantages of both high loading capacity of encapsulated viable cells and good protection during gastrointestinal digestion.
    Language English
    Publishing date 2023-06-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12112256
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Improved viability of probiotics encapsulated in soybean protein isolate matrix microcapsules by coacervation and cross-linking modification

    Hu, Ronghai / Dong, Dejun / Hu, Jielun / Liu, Huan

    Food Hydrocolloids. 2023 May, v. 138 p.108457-

    2023  

    Abstract: The objective of this research was to encapsulate probiotic bacteria based on soy protein isolate (SPI) matrix using either coacervation, cross-linking modification or their combination, and investigate the influences on the survival of probiotic ... ...

    Abstract The objective of this research was to encapsulate probiotic bacteria based on soy protein isolate (SPI) matrix using either coacervation, cross-linking modification or their combination, and investigate the influences on the survival of probiotic bacteria during spray drying, in vitro gastrointestinal digestion, heating, UV irradiation and storage. A probiotic isolate Lactobacillus plantarum 550 was spray dried in SPI solution combined with either pectin (PEC), transglutaminase (TGase) or their combination. High survival rate and low moisture content were obtained for all the samples. Microcapsules fabricated using either SPI-PEC coacervation or TGase induced cross-linking obtained well cell survival during gastric digestion, heat/UV treatment and storage, however, combination of these two modification methods generated microcapsules with lower cross-linking degree and loose package structure corresponding with significant negative effects to the cell viability during gastric digestion and storage. Among the formulations tested, SPI-PEC microparticles provided the best protection to the probiotic cells, as pectin own well thermal stability and digestion resistibility, could fill the cracks and voids existed in SPI matrix and form hydrogen bond to stabilize cell membrane during processing. In conclusion, the current study demonstrated that SPI based probiotic microcapsules with high structural density and cross-linking degree may provide better protection to adverse external environment.
    Keywords Lactobacillus plantarum ; cell membranes ; cell viability ; crosslinking ; digestion ; gastrointestinal system ; heat ; hydrocolloids ; hydrogen bonding ; pectins ; probiotics ; protein-glutamine gamma-glutamyltransferase ; soy protein isolate ; survival rate ; thermal stability ; ultraviolet radiation ; water content ; Microencapsulation ; Probiotic ; Soybean protein isolate ; Pectin ; Transglutaminase ; Spray drying
    Language English
    Dates of publication 2023-05
    Publishing place Elsevier Ltd
    Document type Article ; Online
    ZDB-ID 742742-6
    ISSN 1873-7137 ; 0268-005X
    ISSN (online) 1873-7137
    ISSN 0268-005X
    DOI 10.1016/j.foodhyd.2023.108457
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4414: Show issues

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  4. Article: Extraction and prebiotic potential of beta-glucan from highland barley and its application in probiotic microcapsules

    Yuan, Cong / Hu, Ronghai / He, Li / Hu, Jielun / Liu, Huan

    Food hydrocolloids

    2023  Volume 139, Issue -, Page(s) 108520

    Language English
    Document type Article
    ZDB-ID 742742-6
    ISSN 0268-005X
    Database Current Contents Nutrition, Environment, Agriculture

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    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 4414: Show issues

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  5. Article ; Online: Improved Loading Capacity and Viability of Probiotics Encapsulated in Alginate Hydrogel Beads by In Situ Cultivation Method

    Huang, Yachun / Zhang, Lin / Hu, Jielun / Liu, Huan

    Foods. 2023 June 03, v. 12, no. 11

    2023  

    Abstract: The objective of this research was to encapsulate probiotics by alginate hydrogel beads based on an in situ cultivation method and investigate the influences on the cell loading capacity, surface and internal structure of hydrogel beads and in vitro ... ...

    Abstract The objective of this research was to encapsulate probiotics by alginate hydrogel beads based on an in situ cultivation method and investigate the influences on the cell loading capacity, surface and internal structure of hydrogel beads and in vitro gastrointestinal digestion property of cells. Hydrogel beads were prepared by extrusion and cultured in MRS broth to allow probiotics to grow inside. Up to 10.34 ± 0.02 Log CFU/g of viable cell concentration was obtained after 24 h of in situ cultivation, which broke through the bottleneck of low viable cell counts in the traditional extrusion method. Morphology and rheological analyses showed that the structure of the eventually formed probiotic hydrogel beads can be loosed by the existence of hydrogen bond interaction with water molecules and the internal growth of probiotic microcolonies, while it can be tightened by the acids metabolized by the probiotic bacteria during cultivation. In vitro gastrointestinal digestion analysis showed that great improvement with only 1.09 Log CFU/g of loss in viable cells was found after the entire 6 h of digestion. In conclusion, the current study demonstrated that probiotic microcapsules fabricated by in situ cultivation method have the advantages of both high loading capacity of encapsulated viable cells and good protection during gastrointestinal digestion.
    Keywords alginates ; cell viability ; digestion ; encapsulation ; extrusion ; gastrointestinal system ; hydrogels ; hydrogen bonding ; probiotics
    Language English
    Dates of publication 2023-0603
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12112256
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Effects of Rosa roxburghii & edible fungus fermentation broth on immune response and gut microbiota in immunosuppressed mice

    Xu, Dechang / Hu, Jielun / Zhong, Yadong / Zhang, Yanli / Liu, Wenting / Nie, Shaoping / Xie, Mingyong

    Food Science and Human Wellness. 2024 Jan., v. 13, no. 1 p.154-165

    2024  

    Abstract: With the rise of probiotics fermentation in food industry, fermented foods have attracted worldwide attention. In this study, protective effects of Rosa roxburghii & edible fungus fermentation broth (REFB) on immune function and gut health in ... ...

    Abstract With the rise of probiotics fermentation in food industry, fermented foods have attracted worldwide attention. In this study, protective effects of Rosa roxburghii & edible fungus fermentation broth (REFB) on immune function and gut health in Cyclophosphamide induced immunosuppressed mice were investigated. Results showed that REFB could improve the immune organ index, and promote the proliferation and differentiation of splenic T lymphocytes. In addition, it attenuated intestinal mucosal damage and improved intestinal cellular immunity. REFB administration also up-regulated the expression of IL-4, INF-γ, TNF-α, T-bet and GATA-3 mRNA in small intestine. Furthermore, administration of REFB modulated gut microbiota composition and increased the relative abundance of beneficial genus, such as Bacteroides. It also increased the production of fecal short-chain fatty acids. These indicate that REFB has the potential to improve immunity, alleviate intestinal injury and regulate gut microbiota in immunosuppressed mice.
    Keywords Bacteroides ; GATA transcription factors ; Rosa roxburghii ; cell-mediated immunity ; culture media ; cyclophosphamide ; edible fungi ; fermentation ; food industry ; food science ; humans ; immune response ; interleukin-4 ; intestinal microorganisms ; probiotics ; small intestine ; Fermented foods ; Immunosuppressed mice ; Gut microbiota ; Short-chain fatty acids
    Language English
    Dates of publication 2024-01
    Size p. 154-165.
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 2712869-6
    ISSN 2213-4530
    ISSN 2213-4530
    DOI 10.26599/FSHW.2022.9250013
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: The incorporation of peach gum polysaccharide into soy protein based microparticles improves probiotic bacterial survival during simulated gastrointestinal digestion and storage.

    Yao, Haodong / Liu, Bu / He, Li / Hu, Jielun / Liu, Huan

    Food chemistry

    2023  Volume 413, Page(s) 135596

    Abstract: The objective of this research was to investigate the in vitro gastrointestinal digestion and storage properties of Lactobacillus plantarum 550 encapsulated in soy protein isolate (SPI) and peach gum polysaccharide (PG) through spray drying. High ... ...

    Abstract The objective of this research was to investigate the in vitro gastrointestinal digestion and storage properties of Lactobacillus plantarum 550 encapsulated in soy protein isolate (SPI) and peach gum polysaccharide (PG) through spray drying. High survival rates (>8.1 Log CFU/g) were obtained for all encapsulation formulas containing PG. Combination of SPI and PG showed positive effects on both gastric resistance and storage stability of cells. Among the formulas tested, sample of SPI:PG = 3:1 showed the highest survival (7.88 ± 0.12 Log CFU/g), corresponding to the strongest electrostatic interaction between SPI and PG. With PG content increasing, the storage stability of probiotic was also enhanced, as PG could reduce the moisture content within microcapsules as well as scavenge free radicals generated during storage. In conclusion, the current study demonstrates that SPI combined with PG may provide effective protection to cells not only during spray drying, but also during storage and gastrointestinal digestion.
    MeSH term(s) Soybean Proteins ; Prunus persica ; Microbial Viability ; Bacteria ; Polysaccharides ; Probiotics ; Digestion ; Capsules
    Chemical Substances Soybean Proteins ; Polysaccharides ; Capsules
    Language English
    Publishing date 2023-02-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 243123-3
    ISSN 1873-7072 ; 0308-8146
    ISSN (online) 1873-7072
    ISSN 0308-8146
    DOI 10.1016/j.foodchem.2023.135596
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 3634: Show issues

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  8. Article ; Online: Exercise suppresses neuroinflammation for alleviating Alzheimer's disease.

    Wang, Minghui / Zhang, Hu / Liang, Jiling / Huang, Jielun / Chen, Ning

    Journal of neuroinflammation

    2023  Volume 20, Issue 1, Page(s) 76

    Abstract: Alzheimer's disease (AD) is a chronic neurodegenerative disease, with the characteristics of neurofibrillary tangle (NFT) and senile plaque (SP) formation. Although great progresses have been made in clinical trials based on relevant hypotheses, these ... ...

    Abstract Alzheimer's disease (AD) is a chronic neurodegenerative disease, with the characteristics of neurofibrillary tangle (NFT) and senile plaque (SP) formation. Although great progresses have been made in clinical trials based on relevant hypotheses, these studies are also accompanied by the emergence of toxic and side effects, and it is an urgent task to explore the underlying mechanisms for the benefits to prevent and treat AD. Herein, based on animal experiments and a few clinical trials, neuroinflammation in AD is characterized by long-term activation of pro-inflammatory microglia and the NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasomes. Damaged signals from the periphery and within the brain continuously activate microglia, thus resulting in a constant source of inflammatory responses. The long-term chronic inflammatory response also exacerbates endoplasmic reticulum oxidative stress in microglia, which triggers microglia-dependent immune responses, ultimately leading to the occurrence and deterioration of AD. In this review, we systematically summarized and sorted out that exercise ameliorates AD by directly and indirectly regulating immune response of the central nervous system and promoting hippocampal neurogenesis to provide a new direction for exploring the neuroinflammation activity in AD.
    MeSH term(s) Animals ; Alzheimer Disease ; Inflammasomes/metabolism ; Inflammation/metabolism ; Microglia/metabolism ; Neurodegenerative Diseases/metabolism ; Neurofibrillary Tangles/metabolism ; Neuroinflammatory Diseases ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Exercise ; Humans
    Chemical Substances Inflammasomes ; NLR Family, Pyrin Domain-Containing 3 Protein
    Language English
    Publishing date 2023-03-19
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2156455-3
    ISSN 1742-2094 ; 1742-2094
    ISSN (online) 1742-2094
    ISSN 1742-2094
    DOI 10.1186/s12974-023-02753-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Effects of Lactobacillus casei NCU011054 on immune response and gut microbiota of cyclophosphamide induced immunosuppression mice.

    Min, Fangfang / Hu, Jielun / Huang, Tao / Huang, Yousheng / Nie, Shaoping / Xiong, Tao / Xie, Mingyong

    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

    2023  Volume 174, Page(s) 113662

    Abstract: Lactobacillus (L.) casei NCU011054 isolated from infant feces has been proven to be a potential probiotic in vitro. The present study aimed to investigate the effects of L. casei NCU011054 on the immune response and gut microbiota in cyclophosphamide (CP) ...

    Abstract Lactobacillus (L.) casei NCU011054 isolated from infant feces has been proven to be a potential probiotic in vitro. The present study aimed to investigate the effects of L. casei NCU011054 on the immune response and gut microbiota in cyclophosphamide (CP)-induced immunosuppression mice. Results indicated that L. casei NCU011054 could increase the levels of mucin (Muc2) and tight junction proteins (ZO-1, occludin and claudin-1). Moreover, L. casei NCU011054 was found to upregulate TLRs/NF-κB pathway (TLR-2, TLR-4, TLR-6, p65 and NF-κB) and two transcription factors (T-bet and GATA-3) mRNA levels, and enhance the number of CD4
    MeSH term(s) Animals ; Mice ; NF-kappa B/metabolism ; Lacticaseibacillus casei ; Interleukin-4/metabolism ; Gastrointestinal Microbiome ; Immunity ; Cytokines/metabolism ; Immunosuppression Therapy ; Cyclophosphamide/pharmacology ; Intestinal Diseases
    Chemical Substances NF-kappa B ; Interleukin-4 (207137-56-2) ; Cytokines ; Cyclophosphamide (8N3DW7272P)
    Language English
    Publishing date 2023-02-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 782617-5
    ISSN 1873-6351 ; 0278-6915
    ISSN (online) 1873-6351
    ISSN 0278-6915
    DOI 10.1016/j.fct.2023.113662
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 529: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  10. Article ; Online: Review of structure and bioactivity of the

    Zhang, Shanshan / Hu, Jielun / Sun, Yonggan / Tan, Huizi / Yin, Junyi / Geng, Fang / Nie, Shaoping

    Food chemistry: X

    2021  Volume 12, Page(s) 100158

    Abstract: ... ...

    Abstract Plantago
    Language English
    Publishing date 2021-11-12
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2590-1575
    ISSN (online) 2590-1575
    DOI 10.1016/j.fochx.2021.100158
    Database MEDical Literature Analysis and Retrieval System OnLINE

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