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Article: Prévalence de l’utilisation et de la dépendance aux benzodiazépines au Liban.

Ghayad, T / Dagher, R / Rached, G / Richa, S

2022 Volume 49, Issue 2, Page(s) 197–198

Title translation	Prevalence of benzodiazepine use and dependence in Lebanon.
MeSH term(s)	Humans ; Benzodiazepines/adverse effects ; Prevalence ; Lebanon/epidemiology ; Substance-Related Disorders/epidemiology
Chemical Substances	Benzodiazepines (12794-10-4)
Language	French
Publishing date	2022-08-17
Publishing country	France
Document type	Letter
ZDB-ID	214431-1
ISSN	0013-7006
ISSN	0013-7006
DOI	10.1016/j.encep.2022.05.006
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Article ; Online: Antifungal Activity of the Essential Oil of Origanum syriacum L.

Daouk, Rasha K / Dagher, Shawky M / Sattout, Elsa J

Journal of food protection

2019 Volume 58, Issue 10, Page(s) 1147–1149

Abstract: The volatile oil of the Lebanese Za'atar ( Origanum syriacum L.) was characterized for its thymol ...

Abstract	The volatile oil of the Lebanese Za'atar ( Origanum syriacum L.) was characterized for its thymol and carvacrol content using gas-liquid chromatography. These two compounds constituted the major components of the oil and were present in equal proportions of 30% in the volatile oil extracted from the leaves and shoot tips of the Origanum plant during the preflowering stage. The percentage of carvacrol in the essential oil increased to 62% after flowering and maturation, while the concentration of thymol decreased to 14%. Origanum oil extracted from plants collected during midseason was evaluated for its antifungal activity against Aspergillus niger , Fusarium oxysporum , and Penicillium species. The oil exhibited strong inhibitory action against the three fungi tested. The minimum inhibitory concentration (MIC) of the oil was found to be 0.1 μl/ml of yeast extract sucrose broth for the fungi tested.
Language	English
Publishing date	2019-06-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	243284-5
ISSN	1944-9097 ; 0362-028X
ISSN (online)	1944-9097
ISSN	0362-028X
DOI	10.4315/0362-028X-58.10.1147
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Article ; Online: Peripheral node addressin, a ligand for L-selectin is found in tumor cells and in high endothelial venules in endometrial cancer.

Karpathiou, Georgia / Sramek, Viviana / Dagher, Sami / Mobarki, Mousa / Dridi, Maroa / Picot, Tiphanie / Chauleur, Celine / Peoc'h, Michel

Pathology, research and practice

2022 Volume 233, Page(s) 153888

Abstract: Background: High endothelial venules (HEVs) are vessels specialized in the transport of lymphocytes shown to be implicated in various forms of cancer. They express peripheral node addressin (specifically recognized by the MECA-79 antibody). MECA-79 is ... ...

Abstract	Background: High endothelial venules (HEVs) are vessels specialized in the transport of lymphocytes shown to be implicated in various forms of cancer. They express peripheral node addressin (specifically recognized by the MECA-79 antibody). MECA-79 is also implicated in pregnancy through its expression by epithelial cells of the endometrium. However, the expression of MECA-79 by endothelial or epithelial (cancer) cells has never been studied in endometrial cancer. Material and methods: In this retrospective study, we investigated the immunohistochemical expression of MECA-79 in 40 endometrioid adenocarcinoma hysterectomy specimens and compared it with its expression in 30 non-cancer hysterectomies. Results: HEVs were found in 22% of tumor specimens and in none of the non-cancer hysterectomies (p = 0.005) and were positively associated with higher grade tumors (p = 0.04). MECA-70 was expressed in tumor cells of 70% of carcinomas and in epithelial cells of 46.6% of normal endometria (p = 0.04). It was inversely associated with parametrial invasion (p = 0.03) and larger tumors (statistical trend of p = 0.07). MECA-79 expression was not associated with overall or progression-free survival. Conclusion: MECA-79 is found in HEVs and tumor cells in endometrial endometrioid adenocarcinoma.
MeSH term(s)	Antibodies ; Carcinoma, Endometrioid ; Female ; Humans ; L-Selectin/metabolism ; Ligands ; Retrospective Studies ; Venules
Chemical Substances	Antibodies ; Ligands ; L-Selectin (126880-86-2)
Language	English
Publishing date	2022-04-06
Publishing country	Germany
Document type	Journal Article
ZDB-ID	391889-0
ISSN	1618-0631 ; 0344-0338
ISSN (online)	1618-0631
ISSN	0344-0338
DOI	10.1016/j.prp.2022.153888
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Article ; Online: Arrêt cardiaque par spasme coronaire associé à une maladie de Moyamoya. Apport de l’imagerie cardiaque multimodale.

Cherif, G / Georges, J L / Convers, R / De Malherbe, M / Ajlani, B / Dagher Hayeck, Y / Larnier, L / Blicq, E / Charbonnel, C / Legriel, S / Hervé, D / Livarek, B

Annales de cardiologie et d'angeiologie

2019 Volume 68, Issue 5, Page(s) 375–381

Abstract: Moyamoya disease is a rare angiopathy characterized by a progressive distal occlusion of the internal carotid arteries and their branches. Extracerebral involvement, including coronary arteries, has been described. We report the case of a patient with ... ...

Title translation	Coronary artery spasm revealed by an out-of-hospital cardiac arrest associated with a moyamoya disease. A case report of multimodality imaging.
Abstract	Moyamoya disease is a rare angiopathy characterized by a progressive distal occlusion of the internal carotid arteries and their branches. Extracerebral involvement, including coronary arteries, has been described. We report the case of a patient with moyamoya disease who suffered an out-of-hospital cardiac arrest associated with coronary spasm. We discussed the possible links between coronary spasm and moyamoya, as well as the contribution of multimodal cardiac imaging, combining conventional and intracoronary imaging, cardiac MRI, provocative tests for spasm, in the exploration of out-of-hospital cardiac arrest without obvious electrocardiographic and angiographic cause.
MeSH term(s)	Coronary Vasospasm/diagnostic imaging ; Coronary Vasospasm/etiology ; Humans ; Male ; Middle Aged ; Moyamoya Disease/complications ; Moyamoya Disease/diagnostic imaging ; Multimodal Imaging ; Out-of-Hospital Cardiac Arrest/complications ; Out-of-Hospital Cardiac Arrest/diagnostic imaging
Language	French
Publishing date	2019-08-28
Publishing country	France
Document type	Case Reports ; Journal Article
ZDB-ID	418425-7
ISSN	1768-3181 ; 0003-3928
ISSN (online)	1768-3181
ISSN	0003-3928
DOI	10.1016/j.ancard.2019.07.013
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Article: New records of vascular plants for the flora of Lebanon: a rare species rediscovered after seventy years, Daphne pontica L. (Thymelaeaceae), and three new occurrences, Atropa bella-donna L. (Solanaceae), Circaea lutetiana L. (Onagraceae), and Euonymus latifolius (L.) Mill. (Celastraceae)

El Zein, Hicham / Bou Dagher-Kharrat, Magda

Check list. 2021 Apr. 14, v. 17, no. 2

2021

Abstract: ... occurrences of vascular plants were detected. Atropa bella-donna L. (Solanaceae, Spermatophyte), Circaea ... lutetiana L. (Onagraceae, Spermatophyte), and Euonymus latifolius (L.) Mill. (Celastraceae, Spermatophyte ... were recorded for the first time in Lebanon, while Daphne pontica L. (Thymelaeaceae, Spermatophyte ...

Abstract	During a series of surveys in two valleys of Mount Lebanon between 2014 and 2020, four new occurrences of vascular plants were detected. Atropa bella-donna L. (Solanaceae, Spermatophyte), Circaea lutetiana L. (Onagraceae, Spermatophyte), and Euonymus latifolius (L.) Mill. (Celastraceae, Spermatophyte) were recorded for the first time in Lebanon, while Daphne pontica L. (Thymelaeaceae, Spermatophyte), thought to be extirpated in Lebanon was rediscovered after 70 years. The updated distribution and ecology of these four Euro-Siberian elements, relics of the Quaternary glaciations, are presented.
Keywords	Atropa ; Daphne ; Euonymus ; Onagraceae ; ecology ; flora ; rare species ; Lebanon
Language	English
Dates of publication	2021-0414
Size	p. 655-667.
Publishing place	Pensoft Publishers
Document type	Article
ZDB-ID	2252867-2
ISSN	1809-127X
ISSN	1809-127X
DOI	10.15560/17.2.655
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: In vivo and in vitro anti-inflammatory activity evaluation of Lebanese Cannabis sativa L. ssp. indica (Lam.).

Shebaby, Wassim / Saliba, Jane / Faour, Wissam H / Ismail, Jana / El Hage, Marissa / Daher, Costantine F / Taleb, Robin I / Nehmeh, Bilal / Dagher, Carol / Chrabieh, Edwin / Mroueh, Mohamad

Journal of ethnopharmacology

2020 Volume 270, Page(s) 113743

Abstract: Ethnopharmacological relevance: Cannabis sativa L. is an aromatic annual herb belonging ...

Abstract	Ethnopharmacological relevance: Cannabis sativa L. is an aromatic annual herb belonging to the family Cannabaceae and it is widely distributed worldwide. Cultivation, selling, and consumption of cannabis and cannabis related products, regardless of its use, was prohibited in Lebanon until April 22, 2020. Nevertheless, cannabis oil has been traditionally used unlawfully for many years in Lebanon to treat diseases such as arthritis, diabetes, cancer and few neurological disorders. Aim of the study: The present study aims to evaluate the phytochemical and anti-inflammatory properties of a cannabis oil preparation that is analogous to the illegally used cannabis oil in Lebanon. Materials and methods: Dried Cannabis flowers were extracted with ethanol without any purification procedures to simulate the extracts sold by underground dealers in Lebanon. GC/MS was performed to identify chemical components of the cannabis oil extract (COE). In vivo anti-inflammatory effect of COE was evaluated by using carageenan- and formalin-induced paw edema rat models. TNF-α production were determined by using LPS-activated rat monocytes. Anti-inflammatory markers were quantified using Western blot. Results: Chemical analysis of COE revealed that cannabidiol (CBD; 59.1%) and tetrahydrocannabinol (THC; 20.2%) were found to be the most abundant cannabinoids.Various monoterpenes (α-Pinene, Camphene, β-Myrecene and D-Limonene) and sesquiterpenes (β-Caryophyllene, α-Bergamotene, α-Humelene, Humulene epoxide II, and Caryophyllene oxide) were identified in the extract. Results showed that COE markedly suppressed the release of TNF-α in LPS-stimulated rat monocytes. Western blot analysis revealed that COE significantly inhibited LPS-induced COX-2 and i-NOS protein expressions and blocked the phosphorylation of MAPKs, specifically that of extracellular signal-regulated kinase (ERK), c-Jun NH2-terminal kinase (JNK) and p38 MAPK. COE displayed a significant inhibition of paw edema in both rat models. Histopathological examination revealed that COE reduced inflammation and edema in chronic paw edema model. Conclusion: The current findings demonstrate that COE possesses remarkable in vivo and in vitro anti-inflammatory activities which support the traditional use of the Lebanese cannabis oil extract in the treatment of various inflammatory diseases including arthritis.
MeSH term(s)	Animals ; Anti-Inflammatory Agents/chemistry ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use ; Cannabis/chemistry ; Carrageenan/toxicity ; Disease Models, Animal ; Edema/blood ; Edema/chemically induced ; Edema/drug therapy ; Edema/pathology ; Flowers/chemistry ; Formaldehyde/toxicity ; Inflammation/blood ; Inflammation/chemically induced ; Inflammation/drug therapy ; Inflammation/pathology ; Lebanon ; Lipopolysaccharides/toxicity ; MAP Kinase Signaling System/drug effects ; Male ; Monocytes/drug effects ; Monocytes/metabolism ; Phytochemicals/chemistry ; Phytochemicals/pharmacology ; Phytochemicals/therapeutic use ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Primary Cell Culture ; Rats, Sprague-Dawley ; Tumor Necrosis Factor-alpha/metabolism ; Rats
Chemical Substances	Anti-Inflammatory Agents ; Lipopolysaccharides ; Phytochemicals ; Plant Extracts ; Tumor Necrosis Factor-alpha ; Formaldehyde (1HG84L3525) ; Carrageenan (9000-07-1)
Language	English
Publishing date	2020-12-24
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2020.113743
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Article ; Online: Geographical isolation caused the diversification of the Mediterranean thorny cushion-like Astragalus L. sect. Tragacantha DC. (Fabaceae).

Hardion, Laurent / Dumas, Pierre-Jean / Abdel-Samad, Farah / Bou Dagher Kharrat, Magda / Surina, Bostjan / Affre, Laurence / Médail, Frédéric / Bacchetta, Gianluigi / Baumel, Alex

Molecular phylogenetics and evolution

2016 Volume 97, Page(s) 187–195

Abstract: ... and mountainous opened habitats, the circum-Mediterranean Astragalus L. sect. Tragacantha DC ...

Abstract	Understanding the origin and evolution of Mediterranean vascular flora within the long-term context of climate change requires a continuous study of historical biogeography supported by molecular phylogenetic approaches. Here we provide new insights into the fascinating but often overlooked diversification of Mediterranean xerophytic plants. Growing in some of the most stressing Mediterranean environments, i.e. coastal and mountainous opened habitats, the circum-Mediterranean Astragalus L. sect. Tragacantha DC. (Fabaceae) gathers several thorny cushion-like taxa. These have been the subjects of recent taxonomical studies, but they have not yet been investigated within a comprehensive molecular framework. Bayesian phylogenetics applied to rDNA ITS sequences reveal that the diversification of A. sect. Tragacantha has roots dating back to the Pliocene, and the same data also indicate an eastern-western split giving rise to the five main lineages that exist today. In addition, AFLP fingerprinting supports an old east-west pattern of vicariance that completely rules out the possibility of a recent eastern origin for western taxa. The observed network of genetic relationships implies that contrary to what is widely claimed in the taxonomic literature, it is range fragmentation, as opposed to a coastal-to-mountain ecological shift, that is likely the main driver of diversification.
MeSH term(s)	Amplified Fragment Length Polymorphism Analysis ; Bayes Theorem ; Climate Change ; DNA, Ribosomal/genetics ; Ecosystem ; Evolution, Molecular ; Fabaceae/classification ; Fabaceae/genetics ; Mediterranean Region ; Phylogeny ; Phylogeography
Chemical Substances	DNA, Ribosomal
Language	English
Publishing date	2016-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	33610-5
ISSN	1095-9513 ; 1055-7903
ISSN (online)	1095-9513
ISSN	1055-7903
DOI	10.1016/j.ympev.2016.01.006
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Article: In vivo and in vitro anti-inflammatory activity evaluation of Lebanese Cannabis sativa L. ssp. indica (Lam.)

Shebaby, Wassim / Saliba, Jane / Faour, Wissam H / Ismail, Jana / El Hage, Marissa / Daher, Costantine F / Taleb, Robin I / Nehmeh, Bilal / Dagher, Carol / Chrabieh, Edwin / Mroueh, Mohamad

Journal of ethnopharmacology. 2021 Apr. 24, v. 270

2021

Abstract: Cannabis sativa L. is an aromatic annual herb belonging to the family Cannabaceae and it is widely ...

Abstract	Cannabis sativa L. is an aromatic annual herb belonging to the family Cannabaceae and it is widely distributed worldwide. Cultivation, selling, and consumption of cannabis and cannabis related products, regardless of its use, was prohibited in Lebanon until April 22, 2020. Nevertheless, cannabis oil has been traditionally used unlawfully for many years in Lebanon to treat diseases such as arthritis, diabetes, cancer and few neurological disorders.The present study aims to evaluate the phytochemical and anti-inflammatory properties of a cannabis oil preparation that is analogous to the illegally used cannabis oil in Lebanon.Dried Cannabis flowers were extracted with ethanol without any purification procedures to simulate the extracts sold by underground dealers in Lebanon. GC/MS was performed to identify chemical components of the cannabis oil extract (COE). In vivo anti-inflammatory effect of COE was evaluated by using carageenan- and formalin-induced paw edema rat models. TNF-α production were determined by using LPS-activated rat monocytes. Anti-inflammatory markers were quantified using Western blot.Chemical analysis of COE revealed that cannabidiol (CBD; 59.1%) and tetrahydrocannabinol (THC; 20.2%) were found to be the most abundant cannabinoids.Various monoterpenes (α-Pinene, Camphene, β-Myrecene and D-Limonene) and sesquiterpenes (β-Caryophyllene, α-Bergamotene, α-Humelene, Humulene epoxide II, and Caryophyllene oxide) were identified in the extract. Results showed that COE markedly suppressed the release of TNF-α in LPS-stimulated rat monocytes. Western blot analysis revealed that COE significantly inhibited LPS-induced COX-2 and i-NOS protein expressions and blocked the phosphorylation of MAPKs, specifically that of extracellular signal-regulated kinase (ERK), c-Jun NH2-terminal kinase (JNK) and p38 MAPK. COE displayed a significant inhibition of paw edema in both rat models. Histopathological examination revealed that COE reduced inflammation and edema in chronic paw edema model.The current findings demonstrate that COE possesses remarkable in vivo and in vitro anti-inflammatory activities which support the traditional use of the Lebanese cannabis oil extract in the treatment of various inflammatory diseases including arthritis.
Keywords	Cannabis sativa ; Western blotting ; anti-inflammatory activity ; arthritis ; camphene ; cannabidiol ; diabetes ; edema ; ethanol ; histopathology ; humulene ; inflammation ; limonene ; mitogen-activated protein kinase ; monocytes ; oils ; phosphorylation ; phytochemicals ; rats ; tetrahydrocannabinol ; traditional medicine ; Lebanon
Language	English
Dates of publication	2021-0424
Publishing place	Elsevier B.V.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2020.113743
Database	NAL-Catalogue (AGRICOLA)

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Article: Geographical isolation caused the diversification of the Mediterranean thorny cushion-like Astragalus L. sect. Tragacantha DC. (Fabaceae)

Hardion, Laurent / Alex Baumel / Bostjan Surina / Farah Abdel-Samad / Frédéric Médail / Gianluigi Bacchetta / Laurence Affre / Magda Bou Dagher Kharrat / Pierre-Jean Dumas

Molecular phylogenetics and evolution. 2016 Apr., v. 97

2016

Abstract: ... and mountainous opened habitats, the circum-Mediterranean Astragalus L. sect. Tragacantha DC ...

Abstract	Understanding the origin and evolution of Mediterranean vascular flora within the long-term context of climate change requires a continuous study of historical biogeography supported by molecular phylogenetic approaches. Here we provide new insights into the fascinating but often overlooked diversification of Mediterranean xerophytic plants. Growing in some of the most stressing Mediterranean environments, i.e. coastal and mountainous opened habitats, the circum-Mediterranean Astragalus L. sect. Tragacantha DC. (Fabaceae) gathers several thorny cushion-like taxa. These have been the subjects of recent taxonomical studies, but they have not yet been investigated within a comprehensive molecular framework. Bayesian phylogenetics applied to rDNA ITS sequences reveal that the diversification of A. sect. Tragacantha has roots dating back to the Pliocene, and the same data also indicate an eastern–western split giving rise to the five main lineages that exist today. In addition, AFLP fingerprinting supports an old east–west pattern of vicariance that completely rules out the possibility of a recent eastern origin for western taxa. The observed network of genetic relationships implies that contrary to what is widely claimed in the taxonomic literature, it is range fragmentation, as opposed to a coastal-to-mountain ecological shift, that is likely the main driver of diversification.
Keywords	amplified fragment length polymorphism ; Astragalus ; biogeography ; climate change ; flora ; genetic relationships ; geographical isolation ; habitats ; internal transcribed spacers ; Mediterranean climate ; phylogeny ; ribosomal DNA ; roots ; xerophytes
Language	English
Dates of publication	2016-04
Size	p. 187-195.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	33610-5
ISSN	1095-9513 ; 1055-7903
ISSN (online)	1095-9513
ISSN	1055-7903
DOI	10.1016/j.ympev.2016.01.006
Database	NAL-Catalogue (AGRICOLA)

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Article: Seed germination ecology and salt stress response in eight Mediterranean populations of Sarcopoterium spinosum (L.) Spach

Santo, Andrea / Dessì, Ludovica / Ucchesu, Mariano / Bou Dagher Kharrat, Magda / Charbel Sakr, Ramy / Accogli, Rita A / Buhagiar, Joseph / Kyratzis, Angelos / Fournaraki, Christini / Bacchetta, Gianluigi

Plant species biology. 2019 July, v. 34, no. 3

2019

Abstract: This study aims to deepen the analysis of seed germination ecology and salinity tolerance of Sarcopoterium spinosum (Rosaceae). Germination tests were conducted to evaluate the effect of the fruit’s spongy tissue and the intraspecific variability in seed ...

Abstract	This study aims to deepen the analysis of seed germination ecology and salinity tolerance of Sarcopoterium spinosum (Rosaceae). Germination tests were conducted to evaluate the effect of the fruit’s spongy tissue and the intraspecific variability in seed germination among eight populations of the species on responses to light and total darkness, constant and alternating temperatures, salt stress and germination recovery. The effect of the presence of the spongy tissue varied among populations, with significant results for seed germination. For all populations, optimum germination temperatures were observed in the range of 10–20°C, indicating that S. spinosum and its germination in the field might occur preferably in the period between autumn and early spring. The high water availability due to rainfall during this period could be a considerable advantage for the seed germination of this species. Seeds of S. spinosum showed the ability to germinate in up to 250 mM NaCl in the substrate, and their ability to recover after salt exposure may be interpreted as adaptation to the coastal habitats in which they generally grow. These results give this species a halo‐tolerant character. Great inter‐population variability is detected in this study in several aspects, which indicated that the Mediterranean populations of S. spinosum differ considerably and are adapted to their local conditions. This study provides new information about S. spinosum seed ecology, which could help to preserve and apply effective conservation measures for this species, which in several areas of its distribution range is endangered.
Keywords	Sarcopoterium spinosum ; autumn ; fruits ; habitats ; intraspecific variation ; rain ; salt stress ; salt tolerance ; seed germination ; seeds ; spring ; stress response ; temperature
Language	English
Dates of publication	2019-07
Size	p. 110-121.
Publishing place	John Wiley & Sons Australia, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2020484-X
ISSN	1442-1984 ; 0913-557X
ISSN (online)	1442-1984
ISSN	0913-557X
DOI	10.1111/1442-1984.12244
Database	NAL-Catalogue (AGRICOLA)

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