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Article: Allergen management in the food industry--potential and limitations.

Hischenhuber, Claudia

2005 Volume 49, Issue 1, Page(s) 4–5

MeSH term(s)	Allergens ; European Union ; Food Hypersensitivity/prevention & control ; Food Industry ; Food Labeling/legislation & jurisprudence ; Humans
Chemical Substances	Allergens
Language	English
Publishing date	2005-01
Publishing country	Germany
Document type	Interview
ZDB-ID	2161265-1
ISSN	1613-4125
ISSN	1613-4125
DOI	10.1002/mnfr.200590002
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Article: Food manufacturing initiatives to protect the allergic consumer.

Huggett, A C / Hischenhuber, C

Allergy

1998 Volume 53, Issue 46 Suppl, Page(s) 89–92

MeSH term(s)	Allergens/analysis ; Food Hypersensitivity/prevention & control ; Food Industry ; Food Labeling ; Humans
Chemical Substances	Allergens
Language	English
Publishing date	1998
Publishing country	Denmark
Document type	Journal Article
ZDB-ID	391933-x
ISSN	1398-9995 ; 0105-4538
ISSN (online)	1398-9995
ISSN	0105-4538
DOI	10.1111/j.1398-9995.1998.tb04972.x
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Article: Simplified enzymatic high-performance anion exchange chromatographic determination of total fructans in food and pet food-limitations and measurement uncertainty.

Stöber, Paul / Bénet, Sylvie / Hischenhuber, Claudia

Journal of agricultural and food chemistry

2004 Volume 52, Issue 8, Page(s) 2137–2146

Abstract: A simplified method to determine total fructans in food and pet food has been developed and validated. It follows the principle of AOAC method 997.08, i.e., high-performance anion exchange chromatographic (HPAEC) determination of total fructose released ... ...

Abstract	A simplified method to determine total fructans in food and pet food has been developed and validated. It follows the principle of AOAC method 997.08, i.e., high-performance anion exchange chromatographic (HPAEC) determination of total fructose released from fructans (F(f)) and total glucose released from fructans (G(f)) after enzymatic fructan hydrolysis. Unlike AOAC method 997.08, calculation of total fructans is based on the determination of F(f) alone. This is motivated by the inherent difficulty to accurately determine low amounts of G(f) since many food and pet food products contain other sources of total glucose (e.g., starch and sucrose). In this case, a correction factor g can be used (1.05 by default) to take into account the theoretical contribution of G(f). At levels >5% of total fructans and in commercial fructan ingredients, both F(f) and G(f) can and should be accurately determined; hence, no correction factor g is required. The method is suitable to quantify total fructans in various food and pet food products at concentrations >or=0.2% providing that the product does not contain other significant sources of total fructose such as free fructose or sucrose. Recovery rates in commercial fructan ingredients and in selected food and pet food ranged from 97 to 102%. As part of a measurement uncertainty estimation study, individual contributions to the total uncertainty (u) of the total fructan content were identified and quantified by using the validation data available. As a result, a correlation between the sucrose content and the total uncertainty of the total fructan content was established allowing us to define a limit of quantitation as a function of the sucrose content. One can conclude that this method is limited to food products where the sucrose content does not exceed about three times the total fructan content. Despite this limitation, which is inherent to any total fructan method based on the same approach, this procedure represents an excellent compromise with regard to accuracy, applicability, and convenience.
MeSH term(s)	Animals ; Animals, Domestic ; Chromatography, High Pressure Liquid/methods ; Dietary Carbohydrates/analysis ; Food Analysis/methods ; Fructans/analysis ; Glycoside Hydrolases/metabolism ; Humans ; Polysaccharides/analysis ; Quality Control ; Reproducibility of Results ; Sensitivity and Specificity ; Starch/analysis ; Sucrose/analysis
Chemical Substances	Dietary Carbohydrates ; Fructans ; Polysaccharides ; Sucrose (57-50-1) ; maltodextrin (7CVR7L4A2D) ; Starch (9005-25-8) ; Glycoside Hydrolases (EC 3.2.1.-) ; levanase (EC 3.2.1.65)
Language	English
Publishing date	2004-04-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/jf034770g
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Article: Determination of locust bean gum and guar gum by polymerase chain reaction and restriction fragment length polymorphism analysis.

Meyer, K / Rosa, C / Hischenhuber, C / Meyer, R

Journal of AOAC International

2001 Volume 84, Issue 1, Page(s) 89–99

Abstract: A polymerase chain reaction (PCR) was developed to differentiate the thickening agents locust bean gum (LBG) and the cheaper guar gum in finished food products. Universal primers for amplification of the intergenic spacer region between trnL 3' (UAA) ... ...

Abstract	A polymerase chain reaction (PCR) was developed to differentiate the thickening agents locust bean gum (LBG) and the cheaper guar gum in finished food products. Universal primers for amplification of the intergenic spacer region between trnL 3' (UAA) exon and trnF (GAA) gene in the chloroplast (cp) genome and subsequent restriction analysis were applied to differentiate guar gum and LBG. The presence of <5% (w/w) guar gum powder added to LBG powder was detectable. Based on data obtained from sequencing this intergenic spacer region, a second PCR method for the specific detection of guar gum DNA was also developed. This assay detected guar gum powder in LBG in amounts as low as 1% (w/w). Both methods successfully detected guar gum and/or LBG in ice cream stabilizers and in foodstuffs, such as dairy products, ice cream, dry seasoning mixes, a finished roasting sauce, and a fruit jelly product, but not in products with highly degraded DNA, such as tomato ketchup and sterilized chocolate cream. Both methods detected guar gum and LBG in ice cream and fresh cheese at levels <0.1%.
MeSH term(s)	DNA/analysis ; Electrophoresis, Agar Gel ; Fabaceae/chemistry ; Food Additives/analysis ; Galactans/analysis ; Indicators and Reagents ; Mannans/analysis ; Plant Gums ; Plants, Medicinal ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Polysaccharides/analysis ; Sequence Analysis, DNA
Chemical Substances	Food Additives ; Galactans ; Indicators and Reagents ; Mannans ; Plant Gums ; Polysaccharides ; DNA (9007-49-2) ; guar gum (E89I1637KE) ; locust bean gum (V4716MY704)
Language	English
Publishing date	2001-01
Publishing country	England
Document type	Journal Article
ZDB-ID	1103149-9
ISSN	1944-7922 ; 1060-3271
ISSN (online)	1944-7922
ISSN	1060-3271
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Article: Determination of total taurine in pet foods by liquid chromatography of the dansyl derivative: collaborative study.

McCarthy, K / Hischenhuber, C / Joyce, N / Hischenhuber, C

Journal of AOAC International

2000 Volume 83, Issue 4, Page(s) 784–788

Abstract: A liquid chromatographic (LC) method for the determination of total taurine in pet foods was evaluated in a collaborative study. Ten laboratories assayed 6 blind duplicate pairs of wet and dry pet foods. The taurine in the 6 sample pairs ranged from low ( ...

Abstract	A liquid chromatographic (LC) method for the determination of total taurine in pet foods was evaluated in a collaborative study. Ten laboratories assayed 6 blind duplicate pairs of wet and dry pet foods. The taurine in the 6 sample pairs ranged from low (170 mg/kg) to high (2250 mg/kg) concentrations as is. Collaborators also assayed a sample of known taurine concentration for familiarization purposes. Samples were hydrolyzed to release bound taurine, which was subsequently converted to the dansyl derivative and quantitated by gradient-elution LC with fluorescence detection. Repeatability relative standard deviations, RSDr, ranged from 3.2 to 10.0%; reproducibility relative standard deviations, RSDR, ranged from 6.1 to 16.1%. The method has been adopted Official First Action status by AOAC INTERNATIONAL.
MeSH term(s)	Animals ; Animals, Domestic ; Chromatography, High Pressure Liquid/methods ; Chromatography, High Pressure Liquid/statistics & numerical data ; Dansyl Compounds ; Food Analysis/methods ; Hydrolysis ; Laboratories ; Linear Models ; Reproducibility of Results ; Taurine/analysis
Chemical Substances	Dansyl Compounds ; Taurine (1EQV5MLY3D)
Language	English
Publishing date	2000-07
Publishing country	England
Document type	Journal Article
ZDB-ID	1103149-9
ISSN	1944-7922 ; 1060-3271
ISSN (online)	1944-7922
ISSN	1060-3271
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Article: Increased zinc accumulation in mineralized osteosarcoma tissue measured by confocal synchrotron radiation micro X-ray fluorescence analysis.

Rauwolf, Mirjam / Pemmer, Bernhard / Roschger, Andreas / Turyanskaya, Anna / Smolek, Stephan / Maderitsch, Angelika / Hischenhuber, Peter / Foelser, Martin / Simon, Rolf / Lang, Susanna / Puchner, Stephan E / Windhager, Reinhard / Klaushofer, Klaus / Wobrauschek, Peter / Hofstaetter, Jochen G / Roschger, Paul / Streli, Christina

X-ray spectrometry : XRS

2016 Volume 46, Issue 1, Page(s) 56–62

Abstract: Abnormal tissue levels of certain trace elements such as zinc (Zn) were reported in various types of cancer. Little is known about the role of Zn in osteosarcoma. Using confocal synchrotron radiation micro X-ray fluorescence analysis, we characterized ... ...

Abstract	Abnormal tissue levels of certain trace elements such as zinc (Zn) were reported in various types of cancer. Little is known about the role of Zn in osteosarcoma. Using confocal synchrotron radiation micro X-ray fluorescence analysis, we characterized the spatial distribution of Zn in high-grade sclerosing osteosarcoma of nine patients (four women/five men; seven knee/one humerus/one femur) following chemotherapy and wide surgical resection. Levels were compared with adjacent normal tissue. Quantitative backscattered electron imaging as well as histological examinations was also performed. On average, the ratio of medians of Zn count rates (normalized to calcium) in mineralized tumor tissue was about six times higher than in normal tissue. There was no difference in Zn levels between tumor fraction areas with a low fraction and a high fraction of mineralized tissue, which were clearly depicted using quantitative backscattered electron imaging. Moreover, we found no correlation between the Zn values and the type of tumor regression according to the Salzer-Kuntschik grading. The underlying mechanism of Zn accumulation remains unclear. Given the emerging data on the role of trace elements in other types of cancer, our novel results warrant further studies on the role of trace elements in bone cancer. Copyright © 2016 The Authors.
Language	English
Publishing date	2016-12-21
Publishing country	England
Document type	Journal Article
ZDB-ID	120288-1
ISSN	1097-4539 ; 0049-8246
ISSN (online)	1097-4539
ISSN	0049-8246
DOI	10.1002/xrs.2727
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Article: Criteria for identifying allergenic foods of public health importance.

Björkstén, B / Crevel, R / Hischenhuber, C / Løvik, M / Samuels, F / Strobel, S / Taylor, S L / Wal, J-M / Ward, R

Regulatory toxicology and pharmacology : RTP

2008 Volume 51, Issue 1, Page(s) 42–52

Abstract: The World Health Organisation and other food safety authorities recognise food allergy as a significant public health concern due to the high prevalence and potential severity of the condition and the impact it has on the quality of life and economy. A ... ...

Abstract	The World Health Organisation and other food safety authorities recognise food allergy as a significant public health concern due to the high prevalence and potential severity of the condition and the impact it has on the quality of life and economy. A public health perspective focuses on risk management at the societal level rather than precautions taken by individuals. Allergen lists were originally drawn up on the basis of a combination of prevalence and severity information, but data to document inclusion were limited. Since then the number of allergenic foods for which reactions have been well documented has grown considerably. Yet, most of them are of limited significance to public health. To address food allergy issues from the point of view of risk management, an expert group appointed by the Food Allergy Task Force of the International Life Sciences Institute ILSI Europe reviewed the criteria. We propose a revised set of criteria together with a framework which can be used to help decide which allergenic foods are of sufficient public health importance to be included in allergen lists. Criteria include clinical issues (diagnosis, potency of allergen, severity of reactions), population elements (prevalence, exposure) and modulating factors (food processing). In the framework, data providing evidence for these criteria are weighted according to quality, using a ranking derived from evidence-based medicine. The advantage of this approach is that it makes explicit each of the considerations, thereby rendering the whole process more transparent for all stakeholders.
MeSH term(s)	Allergens/analysis ; Allergens/immunology ; Consensus ; Double-Blind Method ; Environmental Exposure/prevention & control ; Environmental Health ; Food Hypersensitivity/diagnosis ; Food Hypersensitivity/epidemiology ; Food Hypersensitivity/prevention & control ; Humans ; International Cooperation ; Public Health ; Randomized Controlled Trials as Topic ; Risk Assessment
Chemical Substances	Allergens
Language	English
Publishing date	2008-06
Publishing country	Netherlands
Document type	Journal Article ; Multicenter Study
ZDB-ID	604672-1
ISSN	0273-2300
ISSN	0273-2300
DOI	10.1016/j.yrtph.2008.01.002
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Article: Simplified enzymatic high-performance anion exchange chromatographic determination of total fructans in food and pet food--limitations and measurement uncertainty

Stober, P / Benet, S / Hischenhuber, C

Journal of agricultural and food chemistry. 2004 Apr. 21, v. 52, no. 8

2004

Abstract	A simplified method to determine total fructans in food and pet food has been developed and validated. It follows the principle of AOAC method 997.08, i.e., high-performance anion exchange chromatographic (HPAEC) determination of total fructose released from fructans (F(f)) and total glucose released from fructans (G(f)) after enzymatic fructan hydrolysis. Unlike AOAC method 997.08, calculation of total fructans is based on the determination of F(f) alone. This is motivated by the inherent difficulty to accurately determine low amounts of G(f) since many food and pet food products contain other sources of total glucose (e.g., starch and sucrose). In this case, a correction factor g can be used (1.05 by default) to take into account the theoretical contribution of G(f). At levels >5% of total fructans and in commercial fructan ingredients, both F(f) and G(f) can and should be accurately determined; hence, no correction factor g is required. The method is suitable to quantify total fructans in various food and pet food products at concentrations greater than or equal to 0.2% providing that the product does not contain other significant sources of total fructose such as free fructose or sucrose. Recovery rates in commercial fructan ingredients and in selected food and pet food ranged from 97 to 102%. As part of a measurement uncertainty estimation study, individual contributions to the total uncertainty (u) of the total fructan content were identified and quantified by using the validation data available. As a result, a correlation between the sucrose content and the total uncertainty of the total fructan content was established allowing us to define a limit of quantitation as a function of the sucrose content. One can conclude that this method is limited to food products where the sucrose content does not exceed about three times the total fructan content. Despite this limitation, which is inherent to any total fructan method based on the same approach, this procedure represents an excellent compromise with regard to accuracy, applicability, and convenience.
Keywords	pet foods ; infant formulas ; wheat meal ; fructans ; carbohydrate content ; food composition ; feed composition ; enzymatic hydrolysis ; O-glycoside hydrolases ; food analysis ; chromatography ; sucrose ; fructose ; glucose
Language	English
Dates of publication	2004-0421
Size	p. 2137-2146.
Document type	Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
Database	NAL-Catalogue (AGRICOLA)

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Article: Review article: safe amounts of gluten for patients with wheat allergy or coeliac disease.

Hischenhuber, C / Crevel, R / Jarry, B / Mäki, M / Moneret-Vautrin, D A / Romano, A / Troncone, R / Ward, R

Alimentary pharmacology & therapeutics

2006 Volume 23, Issue 5, Page(s) 559–575

Abstract: For both wheat allergy and coeliac disease the dietary avoidance of wheat and other gluten-containing cereals is the only effective treatment. Estimation of the maximum tolerated amount of gluten for susceptible individuals would support effective ... ...

Abstract	For both wheat allergy and coeliac disease the dietary avoidance of wheat and other gluten-containing cereals is the only effective treatment. Estimation of the maximum tolerated amount of gluten for susceptible individuals would support effective management of their disease. Literature was reviewed to evaluate whether an upper limit for gluten content in food, which would be safe for sufferers from both diseases, could be identified. When setting gluten limits for coeliac disease sufferers, the overall potential daily intake should be considered, while for wheat allergy limits should be based on single servings. For coeliac disease sufferers this limit should lie between 10 and 100 mg daily intake. For wheat allergy, lowest eliciting doses for children lie in the lower milligram range, while for adults they are most significantly higher. Gliadins (part of the gluten proteins) not only trigger coeliac disease, but are also major allergens in wheat allergy. Therefore, measurement of gliadins with validated enzyme-linked immunosorbent assay methods provides an appropriate marker for assessing gluten and/or wheat protein contents in food. Available data suggest that a maximum gluten content for 'gluten-free' foods could be set, which protects both wheat allergy sufferers and coeliac patients.
MeSH term(s)	Adult ; Antigens, Plant/immunology ; Autoantigens/immunology ; Biomarkers/analysis ; Celiac Disease/diet therapy ; Celiac Disease/immunology ; Child ; Diet, Protein-Restricted/methods ; Enzyme-Linked Immunosorbent Assay/methods ; Female ; Gliadin/analysis ; Glutens/administration & dosage ; Humans ; Male ; Plant Proteins/immunology ; Prolamins ; Wheat Hypersensitivity/diet therapy ; Wheat Hypersensitivity/immunology
Chemical Substances	Antigens, Plant ; Autoantigens ; Biomarkers ; Plant Proteins ; Prolamins ; Glutens (8002-80-0) ; Gliadin (9007-90-3)
Language	English
Publishing date	2006-03-01
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	639012-2
ISSN	1365-2036 ; 0269-2813 ; 0953-0673
ISSN (online)	1365-2036
ISSN	0269-2813 ; 0953-0673
DOI	10.1111/j.1365-2036.2006.02768.x
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Article: Food authentication by carbohydrate chromatography

Prodolliet, J / Hischenhuber, C

Zeitschrift für Lebensmittel-Untersuchung und -Forschung. A, Food research and technology. 1998. v. 207 (1)

1998

Keywords	honey ; maple syrup ; fruit juices ; mushrooms ; UHT milk ; gums ; coffee (beverage) ; adulterated products ; food analysis ; carbohydrates ; chromatography ; food composition
Language	English
Size	p. 1-12.
Document type	Article
ZDB-ID	1359456-4
ISSN	1431-4630 ; 1438-2377
ISSN	1431-4630 ; 1438-2377
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