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  1. Article ; Online: Tapping the potential of Gram-positive bacteria for bioelectrochemical applications.

    Dundas, Christopher M / Keitz, Benjamin K

    Trends in biotechnology

    2022  Volume 41, Issue 3, Page(s) 273–275

    Abstract: Bacteria that perform extracellular electron transfer (EET) are central to redox-driven biotechnologies, including microbial fuel cells, bioremediation, and bioelectrosynthesis. However, engineerable EET strains have been restricted to well-characterized, ...

    Abstract Bacteria that perform extracellular electron transfer (EET) are central to redox-driven biotechnologies, including microbial fuel cells, bioremediation, and bioelectrosynthesis. However, engineerable EET strains have been restricted to well-characterized, Gram-negative model species. Light et al. identified a previously unknown but widely conserved EET pathway in the Gram-positive bacterium Listeria monocytogenes.
    MeSH term(s) Electron Transport ; Oxidation-Reduction ; Gram-Positive Bacteria ; Bacteria/metabolism ; Biotechnology
    Language English
    Publishing date 2022-12-17
    Publishing country England
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
    ZDB-ID 47474-5
    ISSN 1879-3096 ; 0167-7799
    ISSN (online) 1879-3096
    ISSN 0167-7799
    DOI 10.1016/j.tibtech.2022.12.008
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  2. Article ; Online: Children's Motivation Profiles in Sports and Physical Activities: A Latent Profile Analysis and Self-Determination Theory Approach.

    Lohbeck, Annette / Hohmann, Andreas / von Keitz, Philipp / Daseking, Monika

    Journal of sport & exercise psychology

    2022  Volume 44, Issue 4, Page(s) 251–262

    Abstract: Using latent profile analysis and self-determination theory, the present study aimed to examine younger children's motivation profiles in sports and physical activities and the relations of those profiles to various predictors and achievement outcomes. A ...

    Abstract Using latent profile analysis and self-determination theory, the present study aimed to examine younger children's motivation profiles in sports and physical activities and the relations of those profiles to various predictors and achievement outcomes. A total of 1,116 German children from Grade 2 participated in this study. Latent-profile-analysis solutions based on five behavioral-regulation types covered in self-determination theory (i.e., intrinsic, identified, introjected, external, amotivation) were tested. Results favored a three-profile solution, showing three theoretically meaningful and distinct motivation profiles labeled "amotivated," "non-self-determined," and "self-determined." Older children and children with a lower physical self-concept were more likely to be members of the amotivated profile relative to the other profiles than younger children and children with a higher physical self-concept. Furthermore, children of the self-determined profile demonstrated the best physical performance in various motor-skills tests.
    MeSH term(s) Achievement ; Adolescent ; Child ; Humans ; Motivation ; Personal Autonomy ; Self Concept ; Sports
    Language English
    Publishing date 2022-04-22
    Publishing country United States
    Document type Journal Article
    ISSN 1543-2904
    ISSN (online) 1543-2904
    DOI 10.1123/jsep.2021-0279
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  3. Article ; Online: Tuning Extracellular Electron Transfer by

    Dundas, Christopher M / Walker, David J F / Keitz, Benjamin K

    ACS synthetic biology

    2020  Volume 9, Issue 9, Page(s) 2301–2315

    Abstract: Extracellular electron transfer (EET) pathways, such as those in the ... ...

    Abstract Extracellular electron transfer (EET) pathways, such as those in the bacterium
    MeSH term(s) ATP-Binding Cassette Transporters/genetics ; ATP-Binding Cassette Transporters/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cytochrome c Group/genetics ; Cytochrome c Group/metabolism ; Electron Transport/genetics ; Ferric Compounds/chemistry ; Ferric Compounds/metabolism ; Kinetics ; Logic ; Shewanella/metabolism ; Transcription, Genetic
    Chemical Substances ATP-Binding Cassette Transporters ; Bacterial Proteins ; Cytochrome c Group ; Ferric Compounds ; MtrA protein, Bacteria ; cytochrome c(3) (9035-44-3)
    Language English
    Publishing date 2020-08-17
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 2161-5063
    ISSN (online) 2161-5063
    DOI 10.1021/acssynbio.9b00517
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  4. Article: Children's Physical Self-Concept, Motivation, and Physical Performance: Does Physical Self-Concept or Motivation Play a Mediating Role?

    Lohbeck, Annette / von Keitz, Philipp / Hohmann, Andreas / Daseking, Monika

    Frontiers in psychology

    2021  Volume 12, Page(s) 669936

    Abstract: ... 12 A.M.) by qualified test personnel. Beyond correlation analyses, structural equation modeling (SEM ...

    Abstract The present study aimed to examine the relations between physical self-concept, intrinsic and extrinsic motivation as well as physical performance of 1,082 children aged 7-8 years. The central objective of this study was to contrast a mediation model assuming physical self-concept as a mediator of the relations between both types of motivation and physical performance to a mediation model assuming both types of motivation as mediators of the relations between physical self-concept and physical performance. Physical self-concept and both types of motivation were measured by using self-reported questionnaires, while physical performance was measured with 10 motor skill tests. All tests were carried out during regular school hours (8-12 A.M.) by qualified test personnel. Beyond correlation analyses, structural equation modeling (SEM) was performed to find evidence for the predictive relations between the variables under study. Results showed that physical self-concept was significantly positively related to both types of motivation and physical performance (all
    Language English
    Publishing date 2021-04-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2563826-9
    ISSN 1664-1078
    ISSN 1664-1078
    DOI 10.3389/fpsyg.2021.669936
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  5. Article ; Online: A hybrid transistor with transcriptionally controlled computation and plasticity.

    Gao, Yang / Zhou, Yuchen / Ji, Xudong / Graham, Austin J / Dundas, Christopher M / Miniel Mahfoud, Ismar E / Tibbett, Bailey M / Tan, Benjamin / Partipilo, Gina / Dodabalapur, Ananth / Rivnay, Jonathan / Keitz, Benjamin K

    Nature communications

    2024  Volume 15, Issue 1, Page(s) 1598

    Abstract: Organic electrochemical transistors (OECTs) are ideal devices for translating biological signals into electrical readouts and have applications in bioelectronics, biosensing, and neuromorphic computing. Despite their potential, developing programmable ... ...

    Abstract Organic electrochemical transistors (OECTs) are ideal devices for translating biological signals into electrical readouts and have applications in bioelectronics, biosensing, and neuromorphic computing. Despite their potential, developing programmable and modular methods for living systems to interface with OECTs has proven challenging. Here we describe hybrid OECTs containing the model electroactive bacterium Shewanella oneidensis that enable the transduction of biological computations to electrical responses. Specifically, we fabricated planar p-type OECTs and demonstrated that channel de-doping is driven by extracellular electron transfer (EET) from S. oneidensis. Leveraging this mechanistic understanding and our ability to control EET flux via transcriptional regulation, we used plasmid-based Boolean logic gates to translate biological computation into current changes within the OECT. Finally, we demonstrated EET-driven changes to OECT synaptic plasticity. This work enables fundamental EET studies and OECT-based biosensing and biocomputing systems with genetically controllable and modular design elements.
    MeSH term(s) Electron Transport ; Cell Respiration ; Electricity
    Language English
    Publishing date 2024-02-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-024-45759-1
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  6. Article ; Online: Finding New Meaning in the Practice of Medicine: A Quality Improvement Project Evaluating the Remote Communication Liaison Program During the Initial COVID Pandemic Surge.

    Moraco, Nicole / Lipworth, Adam D / Lei, Yuxiu / Bassell, Lior / Harper, Megan / Collins, Elizabeth / Mosenthal, Anne C / Keitz, Sheri

    Journal of palliative medicine

    2023  Volume 26, Issue 6, Page(s) 807–815

    Abstract: Background: ...

    Abstract Background:
    MeSH term(s) Humans ; United States ; Pandemics ; Quality Improvement ; COVID-19 ; Communication ; Physicians
    Language English
    Publishing date 2023-01-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1427361-5
    ISSN 1557-7740 ; 1096-6218
    ISSN (online) 1557-7740
    ISSN 1096-6218
    DOI 10.1089/jpm.2022.0153
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  7. Article ; Online: Microbial reduction of metal-organic frameworks enables synergistic chromium removal.

    Springthorpe, Sarah K / Dundas, Christopher M / Keitz, Benjamin K

    Nature communications

    2019  Volume 10, Issue 1, Page(s) 5212

    Abstract: Redox interactions between electroactive bacteria and inorganic materials underpin many emerging technologies, but commonly used materials (e.g., metal oxides) suffer from limited tunability and can be challenging to characterize. In contrast, metal- ... ...

    Abstract Redox interactions between electroactive bacteria and inorganic materials underpin many emerging technologies, but commonly used materials (e.g., metal oxides) suffer from limited tunability and can be challenging to characterize. In contrast, metal-organic frameworks exhibit well-defined structures, large surface areas, and extensive chemical tunability, but their utility as microbial substrates has not been examined. Here, we report that metal-organic frameworks can support the growth of the metal-respiring bacterium Shewanella oneidensis, specifically through the reduction of Fe(III). In a practical application, we show that cultures containing S. oneidensis and reduced metal-organic frameworks can remediate lethal concentrations of Cr(VI) over multiple cycles, and that pollutant removal exceeds the performance of either component in isolation or bio-reduced iron oxides. Our results demonstrate that frameworks can serve as growth substrates and suggest that they may offer an alternative to metal oxides in applications seeking to combine the advantages of bacterial metabolism and synthetic materials.
    MeSH term(s) Chromium/isolation & purification ; Metal-Organic Frameworks/chemistry ; Oxidation-Reduction ; Shewanella/genetics ; Shewanella/growth & development ; Shewanella/metabolism
    Chemical Substances Metal-Organic Frameworks ; Chromium (0R0008Q3JB)
    Language English
    Publishing date 2019-11-18
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-019-13219-w
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  8. Article: A Hybrid Transistor with Transcriptionally Controlled Computation and Plasticity.

    Gao, Yang / Zhou, Yuchen / Ji, Xudong / Graham, Austin J / Dundas, Christopher M / Mahfoud, Ismar E Miniel / Tibbett, Bailey M / Tan, Benjamin / Partipilo, Gina / Dodabalapur, Ananth / Rivnay, Jonathan / Keitz, Benjamin K

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Organic electrochemical transistors (OECTs) are ideal devices for translating biological signals into electrical readouts and have applications in bioelectronics, biosensing, and neuromorphic computing. Despite their potential, developing programmable ... ...

    Abstract Organic electrochemical transistors (OECTs) are ideal devices for translating biological signals into electrical readouts and have applications in bioelectronics, biosensing, and neuromorphic computing. Despite their potential, developing programmable and modular methods for living systems to interface with OECTs has proven challenging. Here we describe hybrid OECTs containing the model electroactive bacterium
    Language English
    Publishing date 2023-08-18
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.08.16.553547
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  9. Article: The effect of the design of the orthosis on the axial load transmission of two flexion abduction orthoses used in treating congenital hip dysplasia.

    Schwanitz von Keitz, Paul / Kleimeier, Dana / Lutter, Christoph Fabian / Rehberg, Mirko / Mittelmeier, Wolfram / Kasch, Richard / Osmanski-Zenk, Katrin / Fröhlich, Susanne

    Heliyon

    2022  Volume 8, Issue 12, Page(s) e11942

    Abstract: Background: With an incidence of 2-4% in all newborns, developmental dysplasia of the hip, DDH, represents the most frequent congenital disorder of the skeletal system in Germany. The therapy options are deduced with the help of a sonography. The ... ...

    Abstract Background: With an incidence of 2-4% in all newborns, developmental dysplasia of the hip, DDH, represents the most frequent congenital disorder of the skeletal system in Germany. The therapy options are deduced with the help of a sonography. The conservative therapy approach includes the application of flexion abduction orthoses, which lead to a development of the child's hip through abduction and flexion angle. The overall structure of the orthoses puts a strain on the axial skeleton of the children. The following work is intended to clarify what role the design of the orthoses plays in this respect.
    Methods: Inclusion criterion for the study was fully developed newborns without an indication of skeletal malformations with Type I hip joints according to Graf verified by ultrasound. A total of 19 newborns were recruited and included in the period 3/2013-01/2015. Two types of orthoses used in treating developmental dysplasia of the hip (Tübinger splint, Otto Bock; hip flexion abduction orthosis (Superior orthosis) according to Mittelmeier-Graf, AIDAMED e.K (Kreuz et al., 2012; Mittelmeier et al., 1998; Schmitz et al., 1999), constructions differ, were used. Force was measured with the help of three force sensors, which were even able to be integrated into these without changing the design of the orthosis. In this closed system, force transmission was measured for the duration of a fixed period of two minutes.
    Findings: The greatest axial force development (overall force) is in the Tübinger splint with an average force of 15.1 N (min. 0.59 N, max. 53.09 N, mean 15.1, SD 2.46). 4.09 N (min. 0.96 N, max. 20.99 N, mean 4.09, SD 0.65) resulted in the Superior orthosis. Significant correlations between body weight and resulting axial traction - on average during the entire measurement period and in movement - can be taken from the statistical analysis regarding the Tübinger splint. Such a correlation cannot be depicted for the Superior orthosis.
    Interpretation: The analysis of the load transmission of the examined flexion and abduction orthoses reveals differences between the models. The construct of the orthoses in itself appears to play a significant role. Long-term effects of orthosis therapy on a child's axial skeleton have not been studied to date. Furthermore, it seems reasonable to expand the test series to orthoses, the design of which is configured in a similar matter compared to the examined aids.
    Conclusion: This study proves that the orthotic design has an influence on the infant's axial load.
    Language English
    Publishing date 2022-12-01
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2022.e11942
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  10. Article ; Online: Imposed Environmental Stresses Facilitate Cell-Free Nanoparticle Formation by Deinococcus radiodurans.

    Chen, Angela / Contreras, Lydia M / Keitz, Benjamin K

    Applied and environmental microbiology

    2017  Volume 83, Issue 18

    Abstract: The biological synthesis of metal nanoparticles has been examined in a wide range of organisms, due to increased interest in green synthesis and environmental remediation applications involving heavy metal ion contamination. ...

    Abstract The biological synthesis of metal nanoparticles has been examined in a wide range of organisms, due to increased interest in green synthesis and environmental remediation applications involving heavy metal ion contamination.
    MeSH term(s) Deinococcus/chemistry ; Deinococcus/growth & development ; Deinococcus/metabolism ; Gold/analysis ; Gold/metabolism ; Metal Nanoparticles/analysis ; Silver/analysis ; Silver/metabolism ; Temperature
    Chemical Substances Silver (3M4G523W1G) ; Gold (7440-57-5)
    Language English
    Publishing date 2017-08-31
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/AEM.00798-17
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