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Article ; Conference proceedings: Hashimoto-Thyreoiditis – Risikofaktor für die Entstehung von Schilddrüsenkarzinomen

Geisler, Antje / Becker, Christoph

2024 Volume 103, Issue S 02

Event/congress	95. Jahresversammlung Deutsche Gesellschaft für Hals-Nasen-Ohren-Heilkunde, Kopf- und Hals-Chirurgie e. V., Bonn, Messe Essen, 2024-05-08
Language	German
Publishing date	2024-04-19
Publisher	Georg Thieme Verlag KG
Publishing place	Stuttgart ; New York
Document type	Article ; Conference proceedings
ZDB-ID	96005-6
ISSN	1438-8685 ; 0935-8943 ; 0340-1588
ISSN (online)	1438-8685
ISSN	0935-8943 ; 0340-1588
DOI	10.1055/s-0044-1784048
Database	Thieme publisher's database

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Article ; Conference proceedings: Hashimoto's thyroiditis – Risk factor for the development of thyroid carcinoma

Geisler, Antje / Becker, Christoph

Laryngo-Rhino-Otologie

2024 Volume 103, Issue S 02

Event/congress	95th Annual Meeting German Society of Oto-Rhino-Laryngology, Head and Neck Surgery e. V., Bonn, Messe Essen, 2024-05-08
Language	English
Publishing date	2024-04-19
Publisher	Georg Thieme Verlag KG
Publishing place	Stuttgart ; New York
Document type	Article ; Conference proceedings
ZDB-ID	96005-6
ISSN	1438-8685 ; 0935-8943 ; 0340-1588
ISSN (online)	1438-8685
ISSN	0935-8943 ; 0340-1588
DOI	10.1055/s-0044-1784615
Database	Thieme publisher's database

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Article ; Online: A new approach for detecting adventitious viruses shows Sf-rhabdovirus-negative Sf-RVN cells are suitable for safe biologicals production.

Geisler, Christoph

BMC biotechnology

2018 Volume 18, Issue 1, Page(s) 8

Abstract: Background: Adventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioinformatics tools with massively ... ...

Abstract	Background: Adventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioinformatics tools with massively parallel sequencing technology. Typically, this involves mapping or BLASTN searching individual reads against viral nucleotide databases. Although extremely sensitive for known viruses, this approach can easily miss viruses that are too dissimilar to viruses in the database. Moreover, it is computationally intensive and requires reference cell genome databases. To avoid these drawbacks, we set out to develop an alternative approach. We reasoned that searching genome and transcriptome assemblies for adventitious viral contaminants using TBLASTN with a compact viral protein database covering extant viral diversity as the query could be fast and sensitive without a requirement for high performance computing hardware. Results: We tested our approach on Spodoptera frugiperda Sf-RVN, a recently isolated insect cell line, to determine if it was contaminated with one or more adventitious viruses. We used Illumina reads to assemble the Sf-RVN genome and transcriptome and searched them for adventitious viral contaminants using TBLASTN with our viral protein database. We found no evidence of viral contamination, which was substantiated by the fact that our searches otherwise identified diverse sequences encoding virus-like proteins. These sequences included Maverick, R1 LINE, and errantivirus transposons, all of which are common in insect genomes. We also identified previously described as well as novel endogenous viral elements similar to ORFs encoded by diverse insect viruses. Conclusions: Our results demonstrate TBLASTN searching massively parallel sequencing (MPS) assemblies with a compact, manually curated viral protein database is more sensitive for adventitious virus detection than BLASTN, as we identified various sequences that encoded virus-like proteins, but had no similarity to viral sequences at the nucleotide level. Moreover, searches were fast without requiring high performance computing hardware. Our study also documents the enhanced biosafety profile of Sf-RVN as compared to other Sf cell lines, and supports the notion that Sf-RVN is highly suitable for the production of safe biologicals.
MeSH term(s)	Adenosine Triphosphatases/genetics ; Animals ; Capsid ; Cells, Cultured ; Computational Biology/methods ; DNA Polymerase II/genetics ; DNA Transposable Elements ; Gene Expression Profiling ; Genome, Insect ; Integrases/genetics ; Retroelements/genetics ; Rhabdoviridae/genetics ; Spodoptera/genetics ; Spodoptera/virology ; Viral Proteins/genetics
Chemical Substances	DNA Transposable Elements ; Retroelements ; Viral Proteins ; DNA Polymerase II (EC 2.7.7.-) ; Integrases (EC 2.7.7.-) ; Adenosine Triphosphatases (EC 3.6.1.-)
Language	English
Publishing date	2018-02-07
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ISSN	1472-6750
ISSN (online)	1472-6750
DOI	10.1186/s12896-017-0412-z
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Declining COVID-19 morbidity and case fatality in Germany: the pandemic end?

Dennler, Ulf / Geisler, Fabian / Spinner, Christoph D

Infection

2022 Volume 50, Issue 6, Page(s) 1625–1626

MeSH term(s)	Humans ; Pandemics ; COVID-19/epidemiology ; SARS-CoV-2 ; Germany/epidemiology ; Morbidity
Language	English
Publishing date	2022-06-18
Publishing country	Germany
Document type	Letter
ZDB-ID	185104-4
ISSN	1439-0973 ; 0300-8126 ; 0173-2129
ISSN (online)	1439-0973
ISSN	0300-8126 ; 0173-2129
DOI	10.1007/s15010-022-01873-0
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 881: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article: A new approach for detecting adventitious viruses shows Sf-rhabdovirus-negative Sf-RVN cells are suitable for safe biologicals production

Geisler, Christoph

BMC biotechnology. 2018 Dec., v. 18, no. 1

2018

Abstract: BACKGROUND: Adventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioinformatics tools with massively ... ...

Abstract	BACKGROUND: Adventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioinformatics tools with massively parallel sequencing technology. Typically, this involves mapping or BLASTN searching individual reads against viral nucleotide databases. Although extremely sensitive for known viruses, this approach can easily miss viruses that are too dissimilar to viruses in the database. Moreover, it is computationally intensive and requires reference cell genome databases. To avoid these drawbacks, we set out to develop an alternative approach. We reasoned that searching genome and transcriptome assemblies for adventitious viral contaminants using TBLASTN with a compact viral protein database covering extant viral diversity as the query could be fast and sensitive without a requirement for high performance computing hardware. RESULTS: We tested our approach on Spodoptera frugiperda Sf-RVN, a recently isolated insect cell line, to determine if it was contaminated with one or more adventitious viruses. We used Illumina reads to assemble the Sf-RVN genome and transcriptome and searched them for adventitious viral contaminants using TBLASTN with our viral protein database. We found no evidence of viral contamination, which was substantiated by the fact that our searches otherwise identified diverse sequences encoding virus-like proteins. These sequences included Maverick, R1 LINE, and errantivirus transposons, all of which are common in insect genomes. We also identified previously described as well as novel endogenous viral elements similar to ORFs encoded by diverse insect viruses. CONCLUSIONS: Our results demonstrate TBLASTN searching massively parallel sequencing (MPS) assemblies with a compact, manually curated viral protein database is more sensitive for adventitious virus detection than BLASTN, as we identified various sequences that encoded virus-like proteins, but had no similarity to viral sequences at the nucleotide level. Moreover, searches were fast without requiring high performance computing hardware. Our study also documents the enhanced biosafety profile of Sf-RVN as compared to other Sf cell lines, and supports the notion that Sf-RVN is highly suitable for the production of safe biologicals.
Keywords	Errantivirus ; Spodoptera frugiperda ; bioinformatics ; biosafety ; cell lines ; genetic databases ; genome ; high-throughput nucleotide sequencing ; insect viruses ; insects ; open reading frames ; proteins ; transcriptome ; transposons ; viruses
Language	English
Dates of publication	2018-12
Size	p. 8.
Publishing place	BioMed Central
Document type	Article
ISSN	1472-6750
DOI	10.1186/s12896-017-0412-z
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The Effects of High-Intensity Functional Training Compared with Traditional Strength or Endurance Training on Physical Performance in Adolescents: A Randomized Controlled Trial.

Gavanda, Simon / Isenmann, Eduard / Geisler, Stephan / Faigenbaum, Avery / Zinner, Christoph

Journal of strength and conditioning research

2022 Volume 36, Issue 3, Page(s) 624–632

MeSH term(s)	Adolescent ; Endurance Training ; High-Intensity Interval Training ; Humans ; Muscle Strength ; Physical Endurance ; Physical Functional Performance ; Resistance Training
Language	English
Publishing date	2022-02-18
Publishing country	United States
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	1156349-7
ISSN	1533-4287 ; 1064-8011
ISSN (online)	1533-4287
ISSN	1064-8011
DOI	10.1519/JSC.0000000000004221
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 6263: Show issues

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Article ; Online: A New Bacmid for Customized Protein Glycosylation Pathway Engineering in the Baculovirus-Insect Cell System.

Maghodia, Ajay B / Geisler, Christoph / Jarvis, Donald L

ACS chemical biology

2021 Volume 16, Issue 10, Page(s) 1941–1950

Abstract: One attractive feature of the baculovirus-insect cell system (BICS) is the baculoviral genome has a large capacity for genetic cargo. This enables construction of viral vectors designed to accept multigene insertions, which has facilitated efforts to ... ...

Abstract	One attractive feature of the baculovirus-insect cell system (BICS) is the baculoviral genome has a large capacity for genetic cargo. This enables construction of viral vectors designed to accept multigene insertions, which has facilitated efforts to produce recombinant multisubunit protein complexes. However, the large genetic capacity of baculovirus vectors has not yet been exploited for multistep pathway engineering. Therefore, we created PolyBac, which is a novel baculovirus shuttle vector, or bacmid, that can be used for this purpose. PolyBac was designed to accept multiple transgene insertions by three different mechanisms at three different sites within the baculovirus genome. After constructing and characterizing PolyBac, we used it to isolate nine derivatives encoding various combinations of up to eight different protein
MeSH term(s)	Animals ; Baculoviridae/genetics ; Cell Line ; Genetic Vectors ; Glycosylation ; Hemagglutinin Glycoproteins, Influenza Virus/genetics ; Hemagglutinin Glycoproteins, Influenza Virus/metabolism ; Moths/genetics ; Orthomyxoviridae/chemistry ; Protein Engineering/methods ; Protein Processing, Post-Translational ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism
Chemical Substances	Hemagglutinin Glycoproteins, Influenza Virus ; Recombinant Proteins
Language	English
Publishing date	2021-02-17
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ISSN	1554-8937
ISSN (online)	1554-8937
DOI	10.1021/acschembio.0c00974
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Muscle strength gains per week are higher in the lower-body than the upper-body in resistance training experienced healthy young women-A systematic review with meta-analysis.

Jung, Roger / Gehlert, Sebastian / Geisler, Stephan / Isenmann, Eduard / Eyre, Julia / Zinner, Christoph

PloS one

2023 Volume 18, Issue 4, Page(s) e0284216

Abstract: Background: Women are underrepresented in resistance exercise-related studies. To date only one meta-analysis provides concrete training recommendations for muscle strength gains through resistance training in eumenorrhoeic women.: Objective: This ... ...

Abstract	Background: Women are underrepresented in resistance exercise-related studies. To date only one meta-analysis provides concrete training recommendations for muscle strength gains through resistance training in eumenorrhoeic women. Objective: This review aims to identify research gaps to advance future study in this area to expand the knowledge concerning resistance exercise-induced strength gains in women and to provide guidelines on the number of repetitions per set and the training frequency per week to enhance maximal muscle strength. Methods: The electronic databases PubMed and Web of Science were searched using a comprehensive list of relevant terms. After checking for exclusion criteria, 31 studies could be included in the final analysis using data from 621 subjects. From these data sets, the ideal number of repetitions per set and also the training frequency per week were analyzed. Results: In the lower body, the largest gains were achieved with 1 to 6 repetitions (17.4% 1RM increase). For lower-body exercises, the highest gains were achieved with 13 to 20 repetitions (8.7% 1RM increase). The lower body should be trained two times a week (8.5% 1RM increase). The upper body should be trained two (5.2% 1RM increase) to three times (4.5% 1RM increase) a week. Conclusion: Women can increase their 1RM by 7.2% per week in the upper body and by 5.2% per week in the lower-body exercises. The upper body can be trained more than two times per week whereas the lower body should be trained two times. Women with intermediate experiences in RT and advanced performance level show more rapid increases in strength in the lower-body compared to the upper-body while no differences were found between upper and lower limb adaptations in RT-beginner subjects.
MeSH term(s)	Humans ; Female ; Resistance Training ; Muscle, Skeletal/physiology ; Muscle Strength/physiology ; Exercise ; Adaptation, Physiological
Language	English
Publishing date	2023-04-13
Publishing country	United States
Document type	Meta-Analysis ; Systematic Review ; Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0284216
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: A rare case of a subungual epidermoid cyst treated by surgical excision maintaining maximal functionality.

Geisler, Stephanie / Steinkraus, Volker / Loeser, Christoph R

JAAD case reports

2020 Volume 8, Page(s) 67–70

Language	English
Publishing date	2020-12-27
Publishing country	United States
Document type	Case Reports
ZDB-ID	2834220-3
ISSN	2352-5126
ISSN	2352-5126
DOI	10.1016/j.jdcr.2020.12.018
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Muscle strength gains per week are higher in the lower-body than the upper-body in resistance training experienced healthy young women-A systematic review with meta-analysis.

Roger Jung / Sebastian Gehlert / Stephan Geisler / Eduard Isenmann / Julia Eyre / Christoph Zinner

PLoS ONE, Vol 18, Iss 4, p e

2023 Volume 0284216

Abstract: Background Women are underrepresented in resistance exercise-related studies. To date only one meta-analysis provides concrete training recommendations for muscle strength gains through resistance training in eumenorrhoeic women. Objective This review ... ...

Abstract	Background Women are underrepresented in resistance exercise-related studies. To date only one meta-analysis provides concrete training recommendations for muscle strength gains through resistance training in eumenorrhoeic women. Objective This review aims to identify research gaps to advance future study in this area to expand the knowledge concerning resistance exercise-induced strength gains in women and to provide guidelines on the number of repetitions per set and the training frequency per week to enhance maximal muscle strength. Methods The electronic databases PubMed and Web of Science were searched using a comprehensive list of relevant terms. After checking for exclusion criteria, 31 studies could be included in the final analysis using data from 621 subjects. From these data sets, the ideal number of repetitions per set and also the training frequency per week were analyzed. Results In the lower body, the largest gains were achieved with 1 to 6 repetitions (17.4% 1RM increase). For lower-body exercises, the highest gains were achieved with 13 to 20 repetitions (8.7% 1RM increase). The lower body should be trained two times a week (8.5% 1RM increase). The upper body should be trained two (5.2% 1RM increase) to three times (4.5% 1RM increase) a week. Conclusion Women can increase their 1RM by 7.2% per week in the upper body and by 5.2% per week in the lower-body exercises. The upper body can be trained more than two times per week whereas the lower body should be trained two times. Women with intermediate experiences in RT and advanced performance level show more rapid increases in strength in the lower-body compared to the upper-body while no differences were found between upper and lower limb adaptations in RT-beginner subjects.
Keywords	Medicine ; R ; Science ; Q
Subject code	796
Language	English
Publishing date	2023-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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