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Article ; Online: Stable Laboratory Culture System for the Ctenophore Mnemiopsis leidyi.

Soto-Angel, Joan J / Nordmann, Eva-Lena / Sturm, Daniela / Sachkova, Maria / Pang, Kevin / Burkhardt, Pawel

Methods in molecular biology (Clifton, N.J.)

2024 Volume 2757, Page(s) 123–145

Abstract: Ctenophores are marine organisms attracting significant attention from evolutionary biology, molecular biology, and ecological research. Here, we describe an easy and affordable setup to maintain a stable culture of the ctenophore Mnemiopsis leidyi. The ... ...

Abstract	Ctenophores are marine organisms attracting significant attention from evolutionary biology, molecular biology, and ecological research. Here, we describe an easy and affordable setup to maintain a stable culture of the ctenophore Mnemiopsis leidyi. The challenging delicacy of the lobate ctenophores can be met by monitoring the water quality, providing the right nutrition, and adapting the handling and tank set-up to their fragile gelatinous body plan. Following this protocol allows stable laboratory lines, a continuous supply of embryos for molecular biological studies, and independence from population responses to environmental fluctuations.
MeSH term(s)	Animals ; Ctenophora/physiology
Language	English
Publishing date	2024-04-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3642-8_4
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Article ; Online: Functional properties of Ganoderma lucidum supplementation in canine nutrition.

Kayser, Emanuela / Castaneda, Paola L / Soto-Diaz, Katiria / Steelman, Andrew J / Murphy, Alayna / Spindola, Maria / He, Fei / de Godoy, Maria R C

Journal of animal science

2024 Volume 102

Abstract: Ganoderma lucidum (GL) is a mushroom that has been widely used in Asia for its immunostimulatory and anti-inflammatory capacity, which has been hypothesized to be attributed mainly to the recognition of its cell-surface patterns by cells of the immune ... ...

Abstract	Ganoderma lucidum (GL) is a mushroom that has been widely used in Asia for its immunostimulatory and anti-inflammatory capacity, which has been hypothesized to be attributed mainly to the recognition of its cell-surface patterns by cells of the immune system present in the gastrointestinal tract, resulting in a cascade of modulatory events. However, the nutraceutical properties of GL have not been tested in dogs. Forty adult beagles were used in a completely randomized design. The objective of the present study was to evaluate the effects of dietary inclusion of GL on peripheral blood mononuclear cells (PBMC; T cells, B cells, monocytes, and natural killers), vaccine response, nutrient digestibility, fecal fermentative end-products, and skin and coat quality of adult dogs. Dogs were fed a commercial dry extruded complete and balanced diet plus GL top-dressed daily upon feeding time. Four experimental treatments were used: 0% GL supplementation (control), 5 mg/kg BW of GL, 10 mg/kg BW of GL, or 15 mg/kg BW of GL. Following a 7 d adaptation to the control diet, dogs were fed their respective treatment diets for 28 d. They were challenged with vaccination of a modified live virus Canine Distemper, Adenovirus Type 1 (Hepatitis), Adenovirus Type 2, Parainfluenza, and Parvovirus and killed Rabies Virus on day 7 with blood collections on days 0, 14, and 28. The inclusion of GL in all dosages was well-accepted by all dogs, with no detrimental effect on macronutrient apparent total tract digestibility. There was a trend that the percentage of major histocompatibility II (MHC-II) from B cells was greater in dogs fed 15 mg/kg of GL (41.91%) compared to the control group (34.63%). The phagocytosis response tended to have treatment-by-time interaction among treatments; dogs fed 15 mg/kg of GL tended to have greater phagocytosis activity on day 28 than dogs from the control group and dogs fed 5 mg/kg of GL. The vaccine-specific serum immunoglobulin G (IgG) concentrations were higher in the group supplemented with 15 mg/kg of GL compared to treatment control 7 d after the vaccination for rabies. These data suggest that the inclusion of GL had no detrimental effects on any analyzed PBMC. Due to changes in immune parameters among treatments, GL may also exert beneficial immunostimulatory effects in healthy adult dogs when provided at a daily dose of 15 mg/ kg BW.
MeSH term(s)	Dogs ; Animals ; Digestion ; Leukocytes, Mononuclear ; Reishi ; Feces ; Diet/veterinary ; Dietary Supplements ; Vaccines ; Animal Feed/analysis
Chemical Substances	Vaccines
Language	English
Publishing date	2024-02-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	390959-1
ISSN	1525-3163 ; 0021-8812
ISSN (online)	1525-3163
ISSN	0021-8812
DOI	10.1093/jas/skae051
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Article ; Online: Comfort levels and experiences of middle school and high school age children in anatomical donor-based anatomy outreach sessions.

Asri, Rijul / Baquerizo, Humberto / Padilla-Register, Mercedes / Soto-Greene, Maria L / Grachan, Jeremy J

Anatomical sciences education

2024

Abstract: Many health professions schools host anatomy outreach sessions for high school students that utilize anatomical donors. However, teaching with anatomical donors for younger learners is uncommon. This study aimed to assess the comfort levels and ... ...

Abstract	Many health professions schools host anatomy outreach sessions for high school students that utilize anatomical donors. However, teaching with anatomical donors for younger learners is uncommon. This study aimed to assess the comfort levels and experiences of students who attended the anatomy sessions as part of summer programs at Rutgers New Jersey Medical School. Younger learners (students entering grades 7-10; n = 25) and older learners (students entering grades 11-12; n = 33) completed pre- and post-session surveys about their comfort using a 5-point Likert scale. Before the sessions, most students felt comfortable or very comfortable learning from isolated organs (μ = 2.7, SD = 1.3) or full-body donors (μ = 2.4, SD = 1.4), even though most have never been to an anatomical donor lab before. After the sessions, the comfort level significantly increased for both isolated organs (μ = 3.3, SD = 1.1; p = 0.02) and full-body donors (μ = 3.1, SD = 1.2; p = 0.004). For isolated organs, there was no significant difference in the comfort level between younger and older learners before (p = 0.50) or after (p = 0.56) the sessions. Similarly, for full-body donors, there was no significant difference in the comfort level between younger and older learners before (p = 0.95) or after (p = 0.75) the sessions. Most students expressed that the experience was unique and positive. In conclusion, most students entering grades 7-12 felt comfortable learning from isolated organs and full-body anatomical donors prior to the sessions and felt more comfortable after the sessions. With this, anatomy outreach programs that utilize anatomical donors could be expanded to include younger learners to provide more dynamic teaching experiences for students of various ages.
Language	English
Publishing date	2024-04-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2483491-9
ISSN	1935-9780 ; 1935-9772
ISSN (online)	1935-9780
ISSN	1935-9772
DOI	10.1002/ase.2430
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Article ; Online: Conditions Associated with

Sorrento, Gianluca U / Soto, Maria Carolina Sepulveda / Espay, Alberto J / Bloem, Bastiaan R / Fasano, Alfonso

Movement disorders clinical practice

2022 Volume 9, Issue 4, Page(s) 558–559

Language	English
Publishing date	2022-04-15
Publishing country	United States
Document type	Journal Article
ISSN	2330-1619
ISSN (online)	2330-1619
DOI	10.1002/mdc3.13445
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Article: Bee Assemblage in the Southern Chihuahuan Desert: The Role of Season, Year, and Trap Color in Abundance.

Munguia-Soto, Esteban O / Golubov, Jordan / Mandujano, María C

Insects

2023 Volume 14, Issue 11

Abstract: Recognizing how populations fluctuate over time is a crucial factor in determining the environmental elements affecting population persistence. However, the limited information on wild bee populations complicates the estimation of the impact of ... ...

Abstract	Recognizing how populations fluctuate over time is a crucial factor in determining the environmental elements affecting population persistence. However, the limited information on wild bee populations complicates the estimation of the impact of anthropogenic threats leading to changes in population size. To address this, we conducted a study capturing and monitoring nine species of wild bees through monthly samplings over four years. Tray traps were placed in permanent plots, and capture records were used to determine population size (
Language	English
Publishing date	2023-11-14
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662247-6
ISSN	2075-4450
ISSN	2075-4450
DOI	10.3390/insects14110875
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Article: Synergistic Effects of Co

Machín, Abniel / Morant, Carmen / Soto-Vázquez, Loraine / Resto, Edgard / Ducongé, José / Cotto, María / Berríos-Rolón, Pedro J / Martínez-Perales, Cristian / Márquez, Francisco

Materials (Basel, Switzerland)

2024 Volume 17, Issue 5

Abstract: This research evaluates the efficacy of catalysts based on ... ...

Abstract	This research evaluates the efficacy of catalysts based on Co
Language	English
Publishing date	2024-02-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma17051059
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Article ; Online: Analysis of the quality of e-Consultations in chronic obstructive pulmonary disease.

Álvarez-Dobaño, José M / Rodríguez-García, Carlota / Atienza, Gerardo / Toubes, María E / Rodríguez-Núñez, Nuria / Zamarrón, Carlos / Novo-Platas, José Ángel / Soto-Feijóo, Roi / Landín, Elisa / Carreiras-Cuiña, María / Martínez-Martínez, Honorio J / Carbajales, María Cruz / Otero, Borja / Valdés, Luis

Respiratory medicine

2024 Volume 222, Page(s) 107514

Abstract: Introduction: The quality of e-Consultations in the COPD is unknown. The objectives of this study were (i) to evaluate the quality of referrals; (ii) to define the characteristics of patients referred from Primary Care (PC) to the Unit of Pulmonology; ... ...

Abstract	Introduction: The quality of e-Consultations in the COPD is unknown. The objectives of this study were (i) to evaluate the quality of referrals; (ii) to define the characteristics of patients referred from Primary Care (PC) to the Unit of Pulmonology; and (iii) to describe differences between accepted and rejected patients. Methods: A retrospective, observational study of e-Consultations requested by PC for suspected COPD throughout 2022. To quantify the quality of the e-Consultations, an arbitrary scale of 12 variables (score 0-10) was created. Results: In total, 384 e-Consultations were reviewed, of which 167 (43.5 %) resulted in a face-to-face visit, and 217 (56.5 %) were rejected. No differences were observed between the two types of patients, except for confirmations of diagnostic suspicion of COPD [significantly higher in accepted patients (p = 0.042)]; physical examination data of rejected patients (more data provided; p = 0.015); and lung function (significantly better in rejected patients). The mean quality of referrals was acceptable (5.6 ± 2.1 score): 121 (31.3 %) had insufficient quality; 118 (30.5 %) acceptable; 75 (19.4 %) good, and 30 (7.8 %) excellent. Quality was low in half of the variables analyzed (6/12); acceptable in 3, and good in another 3. The capacity of resolution of referrals was good (one e-Consultation) in 199 requests (66.1 %); deficient (two e-Consultations) in 72 (23.9 %), and poor (≥3 e-Consultations) in 30 (10 %). Overdiagnosis was 40.2 % (86/214 e-Consultations). The risk could be classified in 247 patients (64.3 %; 135 low-risk; 90 high-risk). Conclusions: When adequate information is provided, e-Consultations help identify different levels of severity. However, the quality and capacity of resolution of referrals were suboptimal, with a high percentage of overdiagnoses.
MeSH term(s)	Humans ; Retrospective Studies ; Pulmonary Disease, Chronic Obstructive/diagnosis ; Pulmonary Disease, Chronic Obstructive/epidemiology ; Referral and Consultation
Language	English
Publishing date	2024-01-01
Publishing country	England
Document type	Observational Study ; Journal Article
ZDB-ID	1003348-8
ISSN	1532-3064 ; 0954-6111
ISSN (online)	1532-3064
ISSN	0954-6111
DOI	10.1016/j.rmed.2023.107514
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Article: 'More stressful than cancer': Treatment Experiences Lived During Hurricane Maria among Breast and Colorectal Cancer Patients in Puerto Rico.

Colón-López, Vivian / Sánchez-Cabrera, Yara / Soto-Salgado, Marievelisse / Ortiz-Ortiz, Karen J / Quast, Troy / Fernández, María E

Research square

2023

Abstract: ... regarding their experience with treatment and access to care during and after Hurricanes Irma and Maria and ...

Abstract	Background: This study explored experiences in cancer care and disruption after Hurricanes Irma and Maria's aftermath in Puerto Rico (PR). Methods: A total of three focus groups were conducted among breast and colorectal cancer patients diagnosed six months before the disaster. Results: The most prevalent themes were (a) barriers related to their cancer treatment, (b) facilitators related to their cancer treatment, and (c) treatment experiences during the hurricane. Participants discussed struggles regarding their experience with treatment and access to care during and after Hurricanes Irma and Maria and how household limitations due to lack of electricity and water deter their intention to continue their treatment. Moreover, stressors directly linked with the disaster were the most challenging to cope with. Conclusions: Our study identifies the hardships experienced by cancer patients living during a disaster. Similarly, our study highlights the impending need to address in future emergency plans the individual and system needs of cancer patients in active treatment to minimize the delay in continuing cancer care.
Language	English
Publishing date	2023-04-03
Publishing country	United States
Document type	Preprint
DOI	10.21203/rs.3.rs-2689228/v1
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Article ; Online: Study of the Effect of Water Content in Deep Eutectic Phases on the Extraction of Fatty Acids from Microalgae Biomass

Pedro A. García-Soto / María I. Saavedra de Santiago / María J. Salar-García / Sergio Sánchez-Segado / Víctor M. Ortiz-Martínez

Applied Sciences, Vol 13, Iss 23, p

2023 Volume 12680

Abstract: Microalgae, as some of the oldest life forms on Earth, are of significant interest to industry and in terms of environmental policies, due to their ability to perform photosynthesis and consume atmospheric carbon dioxide. Moreover, they contain a wide ... ...

Abstract	Microalgae, as some of the oldest life forms on Earth, are of significant interest to industry and in terms of environmental policies, due to their ability to perform photosynthesis and consume atmospheric carbon dioxide. Moreover, they contain a wide variety of value-added compounds such as amino acids and proteins, carbohydrates, and fatty acids, which can be exploited in multiple fields like medicine, cosmetics, nutritional supplements, and for the production of biodiesel. In this article, Nannochloropsis gaditana , a type of microalgae that inhabits both fresh and salt water, is studied for fatty acid recovery using deep eutectic solvents (DES). This microalgae species is a natural source of eicosapentaenoic acid (EPA), an omega-3 compound that is commonly used in the nutritional industry. There are numerous extraction techniques and pretreatments to obtain these compounds. In this work, DES are studied as extractive agents due to their advantages as neoteric solvents. Specifically, this work focuses on an assessment of the effect of the composition of DES on the extraction yield of fatty acids from microalgae. Several DES compositions based on choline chloride, ethylene glycol, and fructose are studied to analyze the influence of water content in these phases. The results show that water content significantly influences recovery yields. The DES with higher extractive capacity were those based on choline chloride, ethylene glycol, and water at a molar ratio of 1:2:2. This composition offered 48.7% of the yield obtained with a conventional solvent like methanol for the recovery of EPA (11.2 mg/g microalgae). Furthermore, the choline chloride-fructose-based DES shows the capability of selective extractions of fatty acids with low carbon content—choline chloride:fructose:water (molar ratio 2:1:2) can extract 0.14 mg of decanoic acid/g of microalgae, indicating that this DES composition can recover 35.7% more decanoic acid in comparison to methanol.
Keywords	microalgae ; fatty acids ; omega-3 ; deep eutectic solvents ; green solvents ; biomass valorization ; Technology ; T ; Engineering (General). Civil engineering (General) ; TA1-2040 ; Biology (General) ; QH301-705.5 ; Physics ; QC1-999 ; Chemistry ; QD1-999
Subject code	660
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Sinorhizobium meliloti

Carvia-Hermoso, Cristina / Cuéllar, Virginia / Bernabéu-Roda, Lydia M / van Dillewijn, Pieter / Soto, María J

Plants (Basel, Switzerland)

2024 Volume 13, Issue 5

Abstract: Type IVc Pili (T4cP), also known as Tad or Flp pili, are long thin microbial filaments that are made up of small-sized pilins. These appendages serve different functions in bacteria, including attachment, biofilm formation, surface sensing, motility, and ...

Abstract	Type IVc Pili (T4cP), also known as Tad or Flp pili, are long thin microbial filaments that are made up of small-sized pilins. These appendages serve different functions in bacteria, including attachment, biofilm formation, surface sensing, motility, and host colonization. Despite their relevant role in diverse microbial lifestyles, knowledge about T4cP in bacteria that establish symbiosis with legumes, collectively referred to as rhizobia, is still limited.
Language	English
Publishing date	2024-02-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704341-1
ISSN	2223-7747
ISSN	2223-7747
DOI	10.3390/plants13050628
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