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Article ; Online: Toxicity of

Fernandez-Luna, Maria Teresa / Kumar, Pavan / Hall, David G / Mitchell, Ashaki D / Blackburn, Michael B / Bonning, Bryony C

Toxins

2019 Volume 11, Issue 3

Abstract: The Asian citrus psyllid (ACP), ...

Abstract	The Asian citrus psyllid (ACP),
MeSH term(s)	Animals ; Bacterial Proteins/chemistry ; Bacterial Proteins/toxicity ; Biological Control Agents/chemistry ; Biological Control Agents/toxicity ; Endotoxins/chemistry ; Endotoxins/toxicity ; Feeding Behavior/drug effects ; Hemiptera/drug effects ; Hemiptera/physiology ; Hemolysin Proteins/chemistry ; Hemolysin Proteins/toxicity ; Insecticides/chemistry ; Insecticides/toxicity ; Intestinal Mucosa/drug effects ; Intestinal Mucosa/pathology ; Trypsin/chemistry
Chemical Substances	Bacterial Proteins ; Biological Control Agents ; Endotoxins ; Hemolysin Proteins ; Insecticides ; insecticidal crystal protein, Bacillus Thuringiensis ; Trypsin (EC 3.4.21.4)
Language	English
Publishing date	2019-03-22
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2518395-3
ISSN	2072-6651 ; 2072-6651
ISSN (online)	2072-6651
ISSN	2072-6651
DOI	10.3390/toxins11030173
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Article ; Online: Riboswitch folding: one at a time and step by step.

Fernández-Luna, María Teresa / Miranda-Ríos, Juan

RNA biology

2008 Volume 5, Issue 1, Page(s) 20–23

Abstract: Riboswitches are mRNAs that regulate gene expression upon specific binding of a small metabolite (vitamin cofactors, nucleobases, amino acids, carbohydrates or even metal ions). Riboswitches must fold into very intricate 3D structures to accomplish their ...

Abstract	Riboswitches are mRNAs that regulate gene expression upon specific binding of a small metabolite (vitamin cofactors, nucleobases, amino acids, carbohydrates or even metal ions). Riboswitches must fold into very intricate 3D structures to accomplish their function. Single-molecule studies are very powerful techniques that allow the characterization and elucidation of complex folding paths as well as the identification of intermediate conformational states that an RNA must traverse to acquire its native, functional structure. We review some recent reports on the folding of the adenine-binding riboswitch as studied by fluorescence resonance energy transfer (FRET) and force-measuring optical tweezers (FMOT) techniques.
MeSH term(s)	Adenine/metabolism ; Fluorescence Resonance Energy Transfer ; Nucleic Acid Conformation ; Optical Tweezers ; RNA, Messenger/chemistry ; RNA, Messenger/metabolism
Chemical Substances	RNA, Messenger ; Adenine (JAC85A2161)
Language	English
Publishing date	2008-03-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ISSN	1555-8584
ISSN (online)	1555-8584
DOI	10.4161/rna.5.1.5974
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Article: Riboswitch folding: One at a time and step by step

Fernández-Luna, María Teresa / Miranda-Ríos, Juan

RNA biology. 2008 Jan. 1, v. 5, no. 1

2008

Abstract	Riboswitches are mRNAs that regulate gene expression upon specific binding of a small metabolite (vitamin cofactors, nucleobases, amino acids, carbohydrates or even metal ions). Riboswitches must fold into very intricate 3D structures to accomplish their function. Single-molecule studies are very powerful techniques that allow the characterization and elucidation of complex folding paths as well as the identification of intermediate conformational states that an RNA must traverse to acquire its native, functional structure. We review some recent reports on the folding of the adenine-binding riboswitch as studied by fluorescence resonance energy transfer (FRET) and force-measuring optical tweezers (FMOT) techniques.
Keywords	energy transfer ; fluorescence ; gene expression ; metabolites ; nucleobases
Language	English
Dates of publication	2008-0101
Size	p. 20-23.
Publishing place	Taylor & Francis
Document type	Article
ISSN	1555-8584
DOI	10.4161/rna.5.1.5974
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Toxicity of Bacillus thuringiensis -Derived Pesticidal Proteins Cry1Ab and Cry1Ba against Asian Citrus Psyllid, Diaphorina citri (Hemiptera)

Maria Teresa Fernandez-Luna / Pavan Kumar / David G. Hall / Ashaki D. Mitchell / Michael B. Blackburn / Bryony C. Bonning

Toxins, Vol 11, Iss 3, p

2019 Volume 173

Abstract: The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera), is an important pest of citriculture. The ACP vectors a bacterium that causes huanglongbing (HLB), a devastating and incurable disease of citrus. The bacterium Bacillus thuringiensis ( ...

Abstract	The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera), is an important pest of citriculture. The ACP vectors a bacterium that causes huanglongbing (HLB), a devastating and incurable disease of citrus. The bacterium Bacillus thuringiensis (Bt) produces multiple toxins with activity against a diverse range of insects. In efforts to provide additional control methods for the ACP vector of HLB, we identified pesticidal proteins derived from Bt for toxicity against ACP. The trypsin proteolytic profiles of strain-derived toxins were characterized. Strain IBL-00200, one of six strains with toxins shown to have basal activity against ACP was selected for liquid chromatography-mass spectrometry (LC-MS/MS) identification of the individual Cry toxins expressed. Toxicity assays with individual toxins derived from IBL-00200 were then performed. The activated form of the Cry toxins Cry1Ab and Cry1Ba were toxic to ACP with LC 50 values of approximately 120 µg/mL. Disruption of the midgut epithelium was associated with the toxicity of both the IBL-00200-derived toxin mixture, and with Cry1Ba. With further optimization of the efficacy of Cry1Ab and Cry1Ba, these toxins may have practical utility against ACP. Bt toxins with activity against ACP may provide an additional tool for management of ACP and the associated HLB disease, thereby providing a more sustainable and environmentally benign approach than repeated application of broad-spectrum insecticides.
Keywords	Asian citrus psyllid ; Bacillus thuringiensis ; pesticidal protein ; toxin ; gut epithelium ; Medicine ; R
Subject code	590
Language	English
Publishing date	2019-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: Functional analysis of PTEN variants of unknown significance from PHTS patients unveils complex patterns of PTEN biological activity in disease.

Torices, Leire / Mingo, Janire / Rodríguez-Escudero, Isabel / Fernández-Acero, Teresa / Luna, Sandra / Nunes-Xavier, Caroline E / López, José I / Mercadillo, Fátima / Currás, María / Urioste, Miguel / Molina, María / Cid, Víctor J / Pulido, Rafael

European journal of human genetics : EJHG

2022 Volume 31, Issue 5, Page(s) 568–577

Abstract: Heterozygous germline mutations in PTEN gene predispose to hamartomas and tumors in different tissues, as well as to neurodevelopmental disorders, and define at genetic level the PTEN Hamartoma Tumor Syndrome (PHTS). The major physiologic role of PTEN ... ...

Abstract	Heterozygous germline mutations in PTEN gene predispose to hamartomas and tumors in different tissues, as well as to neurodevelopmental disorders, and define at genetic level the PTEN Hamartoma Tumor Syndrome (PHTS). The major physiologic role of PTEN protein is the dephosphorylation of phosphatidylinositol (3,4,5)-trisphosphate (PIP3), counteracting the pro-oncogenic function of phosphatidylinositol 3-kinase (PI3K), and PTEN mutations in PHTS patients frequently abrogate PTEN PIP3 catalytic activity. PTEN also displays non-canonical PIP3-independent functions, but their involvement in PHTS pathogeny is less understood. We have previously identified and described, at clinical and genetic level, novel PTEN variants of unknown functional significance in PHTS patients. Here, we have performed an extensive functional characterization of these PTEN variants (c.77 C > T, p.(Thr26Ile), T26I; c.284 C > G, p.(Pro95Arg), P95R; c.529 T > A, p.(Tyr177Asn), Y177N; c.781 C > G, p.(Gln261Glu), Q261E; c.829 A > G, p.(Thr277Ala), T277A; and c.929 A > G, p.(Asp310Gly), D310G), including cell expression levels and protein stability, PIP3-phosphatase activity, and subcellular localization. In addition, caspase-3 cleavage analysis in cells has been assessed using a C2-domain caspase-3 cleavage-specific anti-PTEN antibody. We have found complex patterns of functional activity on PTEN variants, ranging from loss of PIP3-phosphatase activity, diminished protein expression and stability, and altered nuclear/cytoplasmic localization, to intact functional properties, when compared with PTEN wild type. Furthermore, we have found that PTEN cleavage at the C2-domain by the pro-apoptotic protease caspase-3 is diminished in specific PTEN PHTS variants. Our findings illustrate the multifaceted molecular features of pathogenic PTEN protein variants, which could account for the complexity in the genotype/phenotype manifestations of PHTS patients.
MeSH term(s)	Humans ; Caspase 3/genetics ; Germ-Line Mutation ; Hamartoma Syndrome, Multiple/genetics ; Phosphatidylinositol 3-Kinases/genetics ; PTEN Phosphohydrolase/genetics ; PTEN Phosphohydrolase/metabolism
Chemical Substances	Caspase 3 (EC 3.4.22.-) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; PTEN Phosphohydrolase (EC 3.1.3.67) ; PTEN protein, human (EC 3.1.3.67)
Language	English
Publishing date	2022-12-21
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1141470-4
ISSN	1476-5438 ; 1018-4813
ISSN (online)	1476-5438
ISSN	1018-4813
DOI	10.1038/s41431-022-01265-w
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Article ; Online: Bt toxin modification for enhanced efficacy.

Deist, Benjamin R / Rausch, Michael A / Fernandez-Luna, Maria Teresa / Adang, Michael J / Bonning, Bryony C

Toxins

2014 Volume 6, Issue 10, Page(s) 3005–3027

Abstract: Insect-specific toxins derived from Bacillus thuringiensis (Bt) provide a valuable resource for pest suppression. Here we review the different strategies that have been employed to enhance toxicity against specific target species including those that ... ...

Abstract	Insect-specific toxins derived from Bacillus thuringiensis (Bt) provide a valuable resource for pest suppression. Here we review the different strategies that have been employed to enhance toxicity against specific target species including those that have evolved resistance to Bt, or to modify the host range of Bt crystal (Cry) and cytolytic (Cyt) toxins. These strategies include toxin truncation, modification of protease cleavage sites, domain swapping, site-directed mutagenesis, peptide addition, and phage display screens for mutated toxins with enhanced activity. Toxin optimization provides a useful approach to extend the utility of these proteins for suppression of pests that exhibit low susceptibility to native Bt toxins, and to overcome field resistance.
MeSH term(s)	Animals ; Bacillus thuringiensis/metabolism ; Bacillus thuringiensis Toxins ; Bacterial Proteins/chemistry ; Biological Control Agents ; Endotoxins/chemistry ; Hemolysin Proteins/chemistry ; Insecta/drug effects ; Mutagenesis, Site-Directed ; Pest Control, Biological ; Protein Conformation
Chemical Substances	Bacillus thuringiensis Toxins ; Bacterial Proteins ; Biological Control Agents ; Endotoxins ; Hemolysin Proteins ; insecticidal crystal protein, Bacillus Thuringiensis
Language	English
Publishing date	2014-10-22
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
ZDB-ID	2518395-3
ISSN	2072-6651 ; 2072-6651
ISSN (online)	2072-6651
ISSN	2072-6651
DOI	10.3390/toxins6103005
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Article: Suppressing the Effect of the Wetting Layer through AlAs Capping in InAs/GaAs QD Structures for Solar Cells Applications.

Ruiz, Nazaret / Fernández, Daniel / Stanojević, Lazar / Ben, Teresa / Flores, Sara / Braza, Verónica / Carro, Alejandro Gallego / Luna, Esperanza / Ulloa, José María / González, David

Nanomaterials (Basel, Switzerland)

2022 Volume 12, Issue 8

Abstract: Recently, thin AlAs capping layers (CLs) on InAs quantum dot solar cells (QDSCs) have been shown to yield better photovoltaic efficiency compared to traditional QDSCs. Although it has been proposed that this improvement is due to the suppression of the ... ...

Abstract	Recently, thin AlAs capping layers (CLs) on InAs quantum dot solar cells (QDSCs) have been shown to yield better photovoltaic efficiency compared to traditional QDSCs. Although it has been proposed that this improvement is due to the suppression of the capture of photogenerated carriers through the wetting layer (WL) states by a de-wetting process, the mechanisms that operate during this process are not clear. In this work, a structural analysis of the WL characteristics in the AlAs/InAs QD system with different CL-thickness has been made by scanning transmission electron microscopy techniques. First, an exponential decline of the amount of InAs in the WL with the CL thickness increase has been found, far from a complete elimination of the WL. Instead, this reduction is linked to a higher shield effect against QD decomposition. Second, there is no compositional separation between the WL and CL, but rather single layer with a variable content of InAlGaAs. Both effects, the high intermixing and WL reduction cause a drastic change in electronic levels, with the CL making up of 1-2 monolayers being the most effective configuration to reduce the radiative-recombination and minimize the potential barriers for carrier transport.
Language	English
Publishing date	2022-04-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662255-5
ISSN	2079-4991
ISSN	2079-4991
DOI	10.3390/nano12081368
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Article: Tailoring of AlAs/InAs/GaAs QDs Nanostructures via Capping Growth Rate.

Ruiz, Nazaret / Fernandez, Daniel / Luna, Esperanza / Stanojević, Lazar / Ben, Teresa / Flores, Sara / Braza, Verónica / Gallego-Carro, Alejandro / Bárcena-González, Guillermo / Yañez, Andres / Ulloa, José María / González, David

Nanomaterials (Basel, Switzerland)

2022 Volume 12, Issue 14

Abstract: The use of thin AlA capping layers (CLs) on InAs quantum dots (QDs) has recently received considerable attention due to improved photovoltaic performance in QD solar cells. However, there is little data on the structural changes that occur during capping ...

Abstract	The use of thin AlA capping layers (CLs) on InAs quantum dots (QDs) has recently received considerable attention due to improved photovoltaic performance in QD solar cells. However, there is little data on the structural changes that occur during capping and their relation to different growth conditions. In this work, we studied the effect of AlA capping growth rate (CGR) on the structural features of InAs QDs in terms of shape, size, density, and average content. As will be shown, there are notable differences in the characteristics of the QDs upon changing CGR. The Al distribution analysis in the CL around the QDs was revealed to be the key. On the one hand, for the lowest CGR, Al has a homogeneous distribution over the entire surface, but there is a large thickening of the CL on the sides of the QD. As a result, the QDs are lower, lenticular in shape, but richer in In. On the other hand, for the higher CGRs, Al accumulates preferentially around the QD but with a more uniform thickness, resulting in taller QDs, which progressively adopt a truncated pyramidal shape. Surprisingly, intermediate CGRs do not improve either of these behaviors, resulting in less enriched QDs.
Language	English
Publishing date	2022-07-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662255-5
ISSN	2079-4991
ISSN	2079-4991
DOI	10.3390/nano12142504
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Article ; Online: Suppressing the Effect of the Wetting Layer through AlAs Capping in InAs/GaAs QD Structures for Solar Cells Applications

Nazaret Ruiz / Daniel Fernández / Lazar Stanojević / Teresa Ben / Sara Flores / Verónica Braza / Alejandro Gallego Carro / Esperanza Luna / José María Ulloa / David González

Nanomaterials, Vol 12, Iss 1368, p

2022 Volume 1368

Abstract	Recently, thin AlAs capping layers (CLs) on InAs quantum dot solar cells (QDSCs) have been shown to yield better photovoltaic efficiency compared to traditional QDSCs. Although it has been proposed that this improvement is due to the suppression of the capture of photogenerated carriers through the wetting layer (WL) states by a de-wetting process, the mechanisms that operate during this process are not clear. In this work, a structural analysis of the WL characteristics in the AlAs/InAs QD system with different CL-thickness has been made by scanning transmission electron microscopy techniques. First, an exponential decline of the amount of InAs in the WL with the CL thickness increase has been found, far from a complete elimination of the WL. Instead, this reduction is linked to a higher shield effect against QD decomposition. Second, there is no compositional separation between the WL and CL, but rather single layer with a variable content of InAlGaAs. Both effects, the high intermixing and WL reduction cause a drastic change in electronic levels, with the CL making up of 1–2 monolayers being the most effective configuration to reduce the radiative-recombination and minimize the potential barriers for carrier transport.
Keywords	InAs quantum dots solar cells ; AlAs capping ; (S)TEM ; Chemistry ; QD1-999
Subject code	530
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: Clinical significance of glycogen synthase kinase 3 (GSK-3) expression and tumor budding grade in colorectal cancer: Implications for targeted therapy.

Guil-Luna, Silvia / Rivas-Crespo, Aurora / Navarrete-Sirvent, Carmen / Mantrana, Ana / Pera, Alejandra / Mena-Osuna, Rafael / Toledano-Fonseca, Marta / García-Ortíz, María Victoria / Villar, Carlos / Sánchez-Montero, Maria Teresa / Krueger, Janna / Medina-Fernández, Francisco Javier / De La Haba-Rodríguez, Juan / Gómez-España, Auxiliadora / Aranda, Enrique / Rudd, Christopher E / Rodríguez-Ariza, Antonio

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

2023 Volume 167, Page(s) 115592

Abstract: Introduction: Glycogen synthase kinase 3 (GSK-3) has been proposed as a novel cancer target due to its regulating role in both tumor and immune cells. However, the connection between GSK-3 and immunoevasive contexture, including tumor budding (TB) has ... ...

Abstract	Introduction: Glycogen synthase kinase 3 (GSK-3) has been proposed as a novel cancer target due to its regulating role in both tumor and immune cells. However, the connection between GSK-3 and immunoevasive contexture, including tumor budding (TB) has not been previously examined. Methods: we investigated the expression levels of total GSK-3 as well as its isoforms (GSK-3β and GSK-3α) and examined their potential correlation with TB grade and the programmed cell death-ligand 1 (PD-L1) in colorectal cancer (CRC) tumor samples. Additionally, we compared the efficacy of GSK-3-inhibition with PD-1/PD-L1 blockade in humanized patient-derived (PDXs) xenografts models of high-grade TB CRC. Results: we show that high-grade (BD3) TB CRC is associated with elevated expression levels of total GSK-3, specifically the GSK-3β isoform, along with increased expression of PD-L1 in tumor cells. Moreover, we define an improved risk stratification of CRC patients based on the presence of GSK-3 Conclusions: our study provides compelling evidence for the clinical significance of GSK-3 expression and TB grade in risk stratification of CRC patients. Moreover, our findings strongly support GSK-3 inhibition as an effective therapy specifically targeting high-grade TB in CRC.
MeSH term(s)	Humans ; CD8-Positive T-Lymphocytes ; Glycogen Synthase Kinase 3 ; Glycogen Synthase Kinase 3 beta ; B7-H1 Antigen ; Programmed Cell Death 1 Receptor ; Clinical Relevance ; Colorectal Neoplasms/pathology
Chemical Substances	Glycogen Synthase Kinase 3 (EC 2.7.11.26) ; Glycogen Synthase Kinase 3 beta (EC 2.7.11.1) ; B7-H1 Antigen ; Programmed Cell Death 1 Receptor
Language	English
Publishing date	2023-09-29
Publishing country	France
Document type	Journal Article
ZDB-ID	392415-4
ISSN	1950-6007 ; 0753-3322 ; 0300-0893
ISSN (online)	1950-6007
ISSN	0753-3322 ; 0300-0893
DOI	10.1016/j.biopha.2023.115592
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