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  1. Article ; Online: Method to share learning in real time at scientific meetings: lessons from the IHI-BMJ International Conference on Quality and Safety.

    Figueroa, Johanna / McPherson, Marianne E / Henriks, Göran / Mountford, James / Barker, Pierre

    BMJ leader

    2024  Volume 8, Issue 1, Page(s) 74–78

    Abstract: Background: Capturing and disseminating key learnings on emerging themes for conference participants is challenging, yet also presents a significant opportunity to distill, share and discuss learning in real time with conference organisers and attendees. ...

    Abstract Background: Capturing and disseminating key learnings on emerging themes for conference participants is challenging, yet also presents a significant opportunity to distill, share and discuss learning in real time with conference organisers and attendees. The Institute for Healthcare Improvement (IHI) and
    Aim: To test a learning system that harvested and synthesised the key lessons shared by conference participants at the 2022 IHI-BMJ Gothenburg Forum, and to disseminate this content.
    Methods: Twelve invited Forum attendees collected and shared their 'breakthrough learnings' via electronic survey. Three IHI team members synthesised the participants' responses into themes that were shared and refined in real time at an in-person Forum session including 35 additional participants.
    Results: Participants shared four learning themes: collaboration and co-production, trust, meaningful communication about data, and broadening the scope of the Science of Improvement field to multi-disciplinary and multi-system approaches.
    Conclusions: Collection of key learning on emerging topics of interest to the health system improvement community is feasible and yielded information both for dissemination and real-time learning. While not representing the full scope of the conference learnings, the content resonated with an additional group of reviewers at the conclusion of the conference and has guided planning for the next annual meeting. This approach may be helpful in capturing key themes for discussion and planning by similar improvement communities.
    MeSH term(s) Humans ; Learning ; Communication ; Health Facilities ; Surveys and Questionnaires
    Language English
    Publishing date 2024-03-18
    Publishing country England
    Document type Journal Article
    ISSN 2398-631X
    ISSN (online) 2398-631X
    DOI 10.1136/leader-2023-000788
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Expression in skin biopsies supports genetic evidence linking CAMKK2, P2X7R and P2X4R with HIV-associated sensory neuropathy.

    Gaff, Jessica / Octaviana, Fitri / Jackaman, Connie / Kamerman, Peter / Papadimitriou, John / Lee, Silvia / Mountford, Jenjira / Price, Patricia

    Journal of neurovirology

    2023  Volume 29, Issue 3, Page(s) 241–251

    Abstract: ... cell counts (r = 0.69, p = 0.004). However, IENFD counts were similar in HIV-SN+ and HIV-SN- donors (p = 0.19 ...

    Abstract HIV-associated sensory neuropathy (HIV-SN) affects 14-38% of HIV+ individuals stable on therapy with no neurotoxic drugs. Polymorphisms in CAMKK2, P2X7R and P2X4R associated with altered risk of HIV-SN in Indonesian and South African patients. The role of CaMKK2 in neuronal repair makes this an attractive candidate, but a direct role for any protein is predicated on expression in affected tissues. Here, we describe expression of CaMKK2, P2X7R and P2X4R proteins in skin biopsies from the lower legs of HIV+ Indonesians with and without HIV-SN, and healthy controls (HC). HIV-SN was diagnosed using the Brief Peripheral Neuropathy Screen. Biopsies were stained to detect protein gene product 9.5 on nerve fibres and CaMKK2, P2X7R or P2X4R, and were examined using 3-colour sequential scanning confocal microscopy. Intraepidermal nerve fibre densities (IENFD) were lower in HIV+ donors than HC and correlated directly with nadir CD4 T-cell counts (r = 0.69, p = 0.004). However, IENFD counts were similar in HIV-SN+ and HIV-SN- donors (p = 0.19) and so did not define neuropathy. CaMKK2+ cells were located close to dermal and epidermal nerve fibres and were rare in HC and HIV-SN- donors, consistent with a role for the protein in nerve damage and/or repair. P2X7R was expressed by cells in blood vessels of HIV-SN- donors, but rarely in HC or HIV-SN+ donors. P2X4R expression by cells in the epidermal basal layer appeared greatest in HIV-SN+ donors. Overall, the differential expression of CaMKK2, P2X7R and P2X4R supports the genetic evidence of a role for these proteins in HIV-SN.
    MeSH term(s) Humans ; HIV Infections/complications ; HIV Infections/genetics ; HIV Infections/diagnosis ; Peripheral Nervous System Diseases/genetics ; Peripheral Nervous System Diseases/complications ; Skin ; Biopsy ; Polymorphism, Single Nucleotide ; Calcium-Calmodulin-Dependent Protein Kinase Kinase/genetics
    Chemical Substances CAMKK2 protein, human (EC 2.7.11.17) ; Calcium-Calmodulin-Dependent Protein Kinase Kinase (EC 2.7.11.17)
    Language English
    Publishing date 2023-05-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1283265-0
    ISSN 1538-2443 ; 1355-0284
    ISSN (online) 1538-2443
    ISSN 1355-0284
    DOI 10.1007/s13365-023-01134-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Structure and Function of the α-Hydroxylation Bimodule of the Mupirocin Polyketide Synthase.

    Winter, Ashley J / Khanizeman, R Nisha / Barker-Mountford, Abigail M C / Devine, Andrew J / Wang, Luoyi / Song, Zhongshu / Davies, Jonathan A / Race, Paul R / Williams, Christopher / Simpson, Thomas J / Willis, Christine L / Crump, Matthew P

    Angewandte Chemie (Weinheim an der Bergstrasse, Germany)

    2023  Volume 135, Issue 47, Page(s) e202312514

    Abstract: Mupirocin is a clinically important antibiotic produced by ... ...

    Abstract Mupirocin is a clinically important antibiotic produced by a
    Language English
    Publishing date 2023-10-16
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 506609-8
    ISSN 1521-3757 ; 0044-8249 ; 0932-2140
    ISSN (online) 1521-3757
    ISSN 0044-8249 ; 0932-2140
    DOI 10.1002/ange.202312514
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Assessing clinician competence in the delivery of cognitive-behavioural therapy for eating disorders: development of the Cognitive-Behavioural Therapy Scale for Eating Disorders (CBTS-ED).

    Beard, Jessica / Cooper, Zafra / Masson, Philip / Mountford, Victoria A / Murphy, Rebecca / Raykos, Bronwyn / Tatham, Madeleine / Thomas, Jennifer J / Turner, Hannah M / Wade, Tracey D / Waller, Glenn

    Cognitive behaviour therapy

    2024  Volume 53, Issue 1, Page(s) 29–47

    Abstract: Evidence-based cognitive-behaviour therapy for eating disorders (CBT-ED) differs from other forms of CBT for psychological disorders, making existing generic CBT measures of therapist competence inadequate for evaluating CBT-ED. This study developed and ... ...

    Abstract Evidence-based cognitive-behaviour therapy for eating disorders (CBT-ED) differs from other forms of CBT for psychological disorders, making existing generic CBT measures of therapist competence inadequate for evaluating CBT-ED. This study developed and piloted the reliability of a novel measure of therapist competence in this domain-the Cognitive Behaviour Therapy Scale for Eating Disorders (CBTS-ED). Initially, a team of CBT-ED experts developed a 26-item measure, with general (i.e. present in every session) and specific (context- or case-dependent) items. To determine statistical properties of the measure, nine CBT-ED experts and eight non-experts independently observed six role-played mock CBT-ED therapy sessions, rating the therapists' performance using the CBTS-ED. The inter-item consistency (Cronbach's alpha and McDonald's omega) and inter-rater reliability (ICC) were assessed, as appropriate to the clustering of the items. The CBTS-ED demonstrated good internal consistency and moderate/good inter-rater reliability for the general items, at least comparable to existing generic CBT scales in other domains. An updated version is proposed, where five of the 16 "specific" items are reallocated to the general group. These preliminary results suggest that the CBTS-ED can be used effectively across both expert and non-expert raters, though less experienced raters might benefit from additional training in its use.
    MeSH term(s) Humans ; Reproducibility of Results ; Cognitive Behavioral Therapy/methods ; Clinical Competence ; Feeding and Eating Disorders/therapy
    Language English
    Publishing date 2024-01-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 2076060-7
    ISSN 1651-2316 ; 1650-6073
    ISSN (online) 1651-2316
    ISSN 1650-6073
    DOI 10.1080/16506073.2023.2263640
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Topic Modeling The Red Pill

    J. B. Mountford

    Social Sciences, Vol 7, Iss 3, p

    2018  Volume 42

    Abstract: The Men’s Rights Activism (MRA) movement and its sub-movement The Red Pill (TRP), has flourished online, offering support and advice to men who feel their masculinity is being challenged by societal shifts. Whilst some insightful studies have been ... ...

    Abstract The Men’s Rights Activism (MRA) movement and its sub-movement The Red Pill (TRP), has flourished online, offering support and advice to men who feel their masculinity is being challenged by societal shifts. Whilst some insightful studies have been carried out, the small samples analysed by researchers limits the scope of studies, which is small compared to the large amounts of data that TRP produces. By extracting a significant quantity of content from a prominent MRA website, ReturnOfKings.com (RoK), whose creator is one of the most prominent figures in the manosphere and who has been featured in multiple studies. Research already completed can be expanded upon with topic modelling and neural networked machine learning, computational analysis that is proposed to augment methodologies of open coding by automatically and unbiasedly analysing conceptual clusters. The successes and limitations of this computational methodology shed light on its further uses in sociological research and has answered the question: What can topic modeling demonstrate about the men’s rights activism movement’s prescriptive masculinity? This methodology not only proved that it could replicate the results of a previous study, but also delivered insights into an increasingly political focus within TRP, and deeper perspectives into the concepts identified within the movement.
    Keywords machine learning ; digital humanities ; men’s rights activism ; masculinities ; online communities ; Social Sciences ; H
    Language English
    Publishing date 2018-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Enhanced short-wavelength sensitivity in the blue-tongued skink Tiliqua rugosa.

    Nagloo, Nicolas / Mountford, Jessica K / Gundry, Ben J / Hart, Nathan S / Davies, Wayne I L / Collin, Shaun P / Hemmi, Jan M

    The Journal of experimental biology

    2022  Volume 225, Issue 11

    Abstract: Despite lizards using a wide range of colour signals, the limited variation in photoreceptor spectral sensitivities across lizards suggests only weak selection for species-specific, spectral tuning of photoreceptors. Some species, however, have enhanced ... ...

    Abstract Despite lizards using a wide range of colour signals, the limited variation in photoreceptor spectral sensitivities across lizards suggests only weak selection for species-specific, spectral tuning of photoreceptors. Some species, however, have enhanced short-wavelength sensitivity, which probably helps with the detection of signals rich in ultraviolet and short wavelengths. In this study, we examined the visual system of Tiliqua rugosa, which has an ultraviolet/blue tongue, to gain insight into this species' visual ecology. We used electroretinograms, opsin sequencing and immunohistochemical labelling to characterize whole-eye spectral sensitivity and the elements that shape it. Our findings reveal that T. rugosa expresses all five opsins typically found in lizards (SWS1, SWS2, RH1, RH2 and LWS) but possesses greatly enhanced short-wavelength sensitivity compared with other diurnal lizards. This enhanced short-wavelength sensitivity is characterized by a broadening of the spectral sensitivity curve of the eye towards shorter wavelengths while the peak sensitivity of the eye at longer wavelengths (560 nm) remains similar to that of other diurnal lizards. While an increased abundance of SWS1 photoreceptors is thought to mediate elevated ultraviolet sensitivity in a couple of other lizard species, SWS1 photoreceptor abundance remains low in this species. Instead, our findings suggest that short-wavelength sensitivity is driven by multiple factors which include a potentially red-shifted SWS1 photoreceptor and the absence of short-wavelength-absorbing oil droplets. Examining the coincidence of enhanced short-wavelength sensitivity with blue tongues among lizards of this genus will provide further insight into the co-evolution of conspecific signals and whole-eye spectral sensitivity.
    MeSH term(s) Animals ; Electroretinography ; Eye ; Lizards ; Opsins/genetics ; Phylogeny
    Chemical Substances Opsins
    Language English
    Publishing date 2022-06-13
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218085-6
    ISSN 1477-9145 ; 0022-0949
    ISSN (online) 1477-9145
    ISSN 0022-0949
    DOI 10.1242/jeb.244317
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Structure and Function of the α-Hydroxylation Bimodule of the Mupirocin Polyketide Synthase.

    Winter, Ashley J / Khanizeman, R Nisha / Barker-Mountford, Abigail M C / Devine, Andrew J / Wang, Luoyi / Song, Zhongshu / Davies, Jonathan A / Race, Paul R / Williams, Christopher / Simpson, Thomas J / Willis, Christine L / Crump, Matthew P

    Angewandte Chemie (International ed. in English)

    2023  Volume 62, Issue 47, Page(s) e202312514

    Abstract: Mupirocin is a clinically important antibiotic produced by a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens. The major bioactive metabolite, pseudomonic acid A (PA-A), is assembled on a tetrasubstituted tetrahydropyran (THP) core ... ...

    Abstract Mupirocin is a clinically important antibiotic produced by a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens. The major bioactive metabolite, pseudomonic acid A (PA-A), is assembled on a tetrasubstituted tetrahydropyran (THP) core incorporating a 6-hydroxy group proposed to be introduced by α-hydroxylation of the thioester of the acyl carrier protein (ACP) bound polyketide chain. Herein, we describe an in vitro approach combining purified enzyme components, chemical synthesis, isotopic labelling, mass spectrometry and NMR in conjunction with in vivo studies leading to the first characterisation of the α-hydroxylation bimodule of the mupirocin biosynthetic pathway. These studies reveal the precise timing of hydroxylation by MupA, substrate specificity and the ACP dependency of the enzyme components that comprise this α-hydroxylation bimodule. Furthermore, using purified enzyme, it is shown that the MmpA KS
    MeSH term(s) Mupirocin ; Polyketide Synthases/metabolism ; Hydroxylation ; Anti-Bacterial Agents/chemistry
    Chemical Substances Mupirocin (D0GX863OA5) ; Polyketide Synthases (79956-01-7) ; Anti-Bacterial Agents
    Language English
    Publishing date 2023-10-17
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.202312514
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Breast Tissue Chemistry Measured In Vivo In Healthy Women Correlate with Breast Density and Breast Cancer Risk.

    Santamaría, Gorane / Naude, Natali / Watson, Julia / Irvine, John / Lloyd, Thomas / Bennett, Ian / Galloway, Graham / Malycha, Peter / Mountford, Carolyn

    Journal of magnetic resonance imaging : JMRI

    2022  Volume 56, Issue 5, Page(s) 1355–1369

    Abstract: Background: The relationship of tissue chemistry to breast density and cancer risk has not been documented despite breast density being a known risk factor.: Purpose: To investigate whether distinct chemical profiles associated with breast density ... ...

    Abstract Background: The relationship of tissue chemistry to breast density and cancer risk has not been documented despite breast density being a known risk factor.
    Purpose: To investigate whether distinct chemical profiles associated with breast density and cancer risk are identified in healthy breast tissue using in vivo two-dimensional correlated spectroscopy (2D COSY).
    Study type: Prospective.
    Population: One-hundred-seven participants including 55 at low risk and 52 at high risk of developing breast cancer.
    Field strength/sequence: 3 T/ axial/ T1, T2, 2D COSY.
    Assessment: Two radiologists defined breast density on T2. Interobserver variability assessed. Peak volumes normalized to methylene at (1.30, 1.30) ppm as internal shift reference.
    Statistical tests: Chi-squared/Mann-Whitney/Kappa statistics/Kruskal Wallis/pairwise analyses. Significance level 0.05.
    Results: Ten percentage were fatty breasts, 39% scattered fibroglandular, 35% heterogeneously dense, and 16% extremely dense. Interobserver variability was excellent (kappa = 0.817). Sixty percentage (64/107) were premenopausal. Four distinct tissue chemistry categories were identified: low-density (LD)/premenopausal, high-density (HD)/premenopausal, LD/postmenopausal, and HD/postmenopausal. Compared to LD, HD breast chemistry showed significant increases of cholesterol (235%) and lipid unsaturation (33%). In the low-risk category, postmenopausal women with dense breasts recorded the largest significant changes including cholesterol methyl 540%, lipid unsaturation 207%, glutamine/glutamate 900%, and choline/phosphocholine 800%. In the high-risk cohort, premenopausal women with HD recorded a more active chemical profile with significant increases in choline/phosphocholine 1100%, taurine/glucose 550% and cholesterol sterol 250%.
    Data conclusion: Four distinct chemical profiles were identified in healthy breast tissue based on breast density and menopausal status in participants at low and high risk. Gradual increase in neutral lipid content and metabolites was noted in both risk groups across categories in different order. In low risk, the HD postmenopausal category exhibited the highest metabolic activity, while women at high risk exhibited the highest lipid content and metabolic activity in the HD premenopausal category.
    Level of evidence: 2 TECHNICAL EFFICACY STAGE: 3.
    MeSH term(s) Breast Density ; Breast Neoplasms/diagnostic imaging ; Choline ; Female ; Glucose ; Glutamates ; Glutamine ; Humans ; Lipids ; Mammography ; Phosphorylcholine ; Prospective Studies ; Risk Factors ; Sterols ; Taurine
    Chemical Substances Glutamates ; Lipids ; Sterols ; Glutamine (0RH81L854J) ; Phosphorylcholine (107-73-3) ; Taurine (1EQV5MLY3D) ; Glucose (IY9XDZ35W2) ; Choline (N91BDP6H0X)
    Language English
    Publishing date 2022-03-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1146614-5
    ISSN 1522-2586 ; 1053-1807
    ISSN (online) 1522-2586
    ISSN 1053-1807
    DOI 10.1002/jmri.28168
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Curious case of gut dysmotility.

    Lee, Phey Shen / Jopson, Laura / Needham, Stephanie J / Mountford, Christopher G / Thompson, Nick P

    Gut

    2019  Volume 70, Issue 1, Page(s) 66–126

    MeSH term(s) Aged ; Amyloidosis/complications ; Amyloidosis/diagnosis ; Gastrointestinal Diseases/complications ; Gastrointestinal Diseases/diagnosis ; Gastrointestinal Motility ; Humans ; Male ; Multiple Myeloma/complications ; Multiple Myeloma/diagnosis
    Language English
    Publishing date 2019-11-08
    Publishing country England
    Document type Case Reports ; Journal Article
    ZDB-ID 80128-8
    ISSN 1468-3288 ; 0017-5749
    ISSN (online) 1468-3288
    ISSN 0017-5749
    DOI 10.1136/gutjnl-2019-319840
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Extracellular vesicles from differentiated stem cells contain novel proangiogenic miRNAs and induce angiogenic responses at low doses.

    Kesidou, Despoina / Bennett, Matthew / Monteiro, João P / McCracken, Ian R / Klimi, Eftychia / Rodor, Julie / Condie, Alison / Cowan, Scott / Caporali, Andrea / Wit, Jan B M / Mountford, Joanne C / Brittan, Mairi / Beqqali, Abdelaziz / Baker, Andrew H

    Molecular therapy : the journal of the American Society of Gene Therapy

    2023  Volume 32, Issue 1, Page(s) 185–203

    Abstract: Extracellular vesicles (EVs) released from healthy endothelial cells (ECs) have shown potential for promoting angiogenesis, but their therapeutic efficacy remains poorly understood. We have previously shown that transplantation of a human embryonic stem ... ...

    Abstract Extracellular vesicles (EVs) released from healthy endothelial cells (ECs) have shown potential for promoting angiogenesis, but their therapeutic efficacy remains poorly understood. We have previously shown that transplantation of a human embryonic stem cell-derived endothelial cell product (hESC-ECP), promotes new vessel formation in acute ischemic disease in mice, likely via paracrine mechanism(s). Here, we demonstrated that EVs from hESC-ECPs (hESC-eEVs) significantly increased EC tube formation and wound closure in vitro at ultralow doses, whereas higher doses were ineffective. More important, EVs isolated from the mesodermal stage of the differentiation (hESC-mEVs) had no effect. Small RNA sequencing revealed that hESC-eEVs have a unique transcriptomic profile and are enriched in known proangiogenic microRNAs (miRNAs, miRs). Moreover, an in silico analysis identified three novel hESC-eEV-miRNAs with potential proangiogenic function. Differential expression analysis suggested that two of those, miR-4496 and miR-4691-5p, are highly enriched in hESC-eEVs. Overexpression of miR-4496 or miR-4691-5p resulted in increased EC tube formation and wound closure in vitro, validating the novel proangiogenic function of these miRNAs. In summary, we demonstrated that hESC-eEVs are potent inducers of EC angiogenic response at ultralow doses and contain a unique EV-associated miRNA repertoire, including miR-4496 and miR-4691-5p, with novel proangiogenic function.
    MeSH term(s) Humans ; Animals ; Mice ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Endothelial Cells/metabolism ; Extracellular Vesicles/genetics ; Extracellular Vesicles/metabolism ; Cell Differentiation/genetics ; Stem Cells/metabolism
    Chemical Substances MicroRNAs
    Language English
    Publishing date 2023-12-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2010592-7
    ISSN 1525-0024 ; 1525-0016
    ISSN (online) 1525-0024
    ISSN 1525-0016
    DOI 10.1016/j.ymthe.2023.11.023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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