Article ; Online: A mechanistic insight on how Compromised Hydrolysis of Triacylglycerol 7 (CHT7) restrains the involvement of it's CXC domain from quiescence repression.
International journal of biological macromolecules
2024 Volume 265, Issue Pt 1, Page(s) 130844
Abstract: CHT7 is a regulator of quiescence repression in Chlamydomonas reinhardtii. Initially, CHT7's repression activity was thought to be managed by its DNA-binding CXC domain. Later, it was found that the CHT7-CXC domain is dispensable for CHT7's activities. ... ...
| Abstract | CHT7 is a regulator of quiescence repression in Chlamydomonas reinhardtii. Initially, CHT7's repression activity was thought to be managed by its DNA-binding CXC domain. Later, it was found that the CHT7-CXC domain is dispensable for CHT7's activities. Rather, CHT7's predicted protein domains were proposed to be involved in regulation activities by binding to other repressors in the cell. Yet, it remains unclear why and how CHT7 refrains its CXC domain from participating in any transcriptional activities. The question becomes more intriguing, since CXC binding regions are available in promoter regions of some of the misregulated genes in CHT7 mutant (cht7). Through biophysical experiments and molecular dynamics approaches, we studied the DNA recognition behavior of CHT7-CXC. The results indicate that this domain possesses sequence selectivity due to the differential binding abilities of its subdomains. Further, to understand if the case is that CXC loses its DNA binding capabilities in the vicinity of other repressors, we examined CHT7-CXC's DNA binding stability under the spatial constraint conditions created through fusing CHT7-CXC with AsLOV2. The results show limited ability of CHT7-CXC to withstand steric forces and provide insights to why and how algal cells may hold back CHT7-CXC's indulgence in quiescence repression. CLASSIFICATIONS: Biological Sciences, Biophysics and Computational Biology. |
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| MeSH term(s) | Triglycerides ; Hydrolysis ; Transcription Factors/genetics ; DNA-Binding Proteins/metabolism ; DNA ; Transcription, Genetic |
| Chemical Substances | Triglycerides ; Transcription Factors ; DNA-Binding Proteins ; DNA (9007-49-2) |
| Language | English |
| Publishing date | 2024-03-13 |
| Publishing country | Netherlands |
| Document type | Journal Article |
| ZDB-ID | 282732-3 |
| ISSN | 1879-0003 ; 0141-8130 |
| ISSN (online) | 1879-0003 |
| ISSN | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2024.130844 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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