Article ; Online: Structural insights into the multivalent binding of the Arabidopsis FLOWERING LOCUS T promoter by the CO-NF-Y master transcription factor complex.
2021 Volume 33, Issue 4, Page(s) 1182–1195
Abstract: ... in the photoperiod floral pathway that integrates upstream signals and activates the florigen gene FLOWERING LOCUS T ... gives the CO-NF-Y complex high affinity and specificity for FT promoter binding. Overall, our data ... NF-Y during the floral transition. ...
| Abstract | Flowering plants sense various environmental and endogenous signals to trigger the floral transition and start the reproductive growth cycle. CONSTANS (CO) is a master transcription factor in the photoperiod floral pathway that integrates upstream signals and activates the florigen gene FLOWERING LOCUS T (FT). Here, we performed comprehensive structural and biochemical analyses to study the molecular mechanism underlying the regulation of FT by CO in Arabidopsis thaliana. We show that the four previously characterized cis-elements in the FT promoter proximal region, CORE1, CORE2, P1, and P2, are all direct CO binding sites. Structural analysis of CO in complex with NUCLEAR FACTOR-YB/YC (NF-YB/YC) and the CORE2 or CORE1 elements revealed the molecular basis for the specific recognition of the shared TGTG motifs. Biochemical analysis suggested that CO might form a homomultimeric assembly via its N-terminal B-Box domain and simultaneously occupy multiple cis-elements within the FT promoter. We suggest that this multivalent binding gives the CO-NF-Y complex high affinity and specificity for FT promoter binding. Overall, our data provide a detailed molecular model for the regulation of FT by the master transcription factor complex CO-NF-Y during the floral transition. |
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| MeSH term(s) | Arabidopsis/genetics ; Arabidopsis/metabolism ; Arabidopsis Proteins/chemistry ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Binding Sites ; Crystallography, X-Ray ; DNA-Binding Proteins/chemistry ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Gene Expression Regulation, Plant ; Multiprotein Complexes/chemistry ; Multiprotein Complexes/genetics ; Multiprotein Complexes/metabolism ; Promoter Regions, Genetic ; Protein Domains ; Trans-Activators/chemistry ; Trans-Activators/genetics ; Trans-Activators/metabolism ; Transcription Factors/chemistry ; Transcription Factors/genetics ; Transcription Factors/metabolism |
| Chemical Substances | Arabidopsis Proteins ; At4g14540 protein, Arabidopsis ; CONSTANS protein, Arabidopsis ; DNA-Binding Proteins ; FT protein, Arabidopsis ; Multiprotein Complexes ; NF-YC4 protein, Arabidopsis ; Trans-Activators ; Transcription Factors |
| Language | English |
| Publishing date | 2021-03-06 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 623171-8 |
| ISSN | 1532-298X ; 1040-4651 |
| ISSN (online) | 1532-298X |
| ISSN | 1040-4651 |
| DOI | 10.1093/plcell/koab016 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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