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  1. Article: Serum Allergen-Specific Immunoglobulin E in Cats with Inflammatory Bronchial Disease.

    Hörner-Schmid, Lina / Palić, Jelena / Mueller, Ralf S / Schulz, Bianka

    Animals : an open access journal from MDPI

    2023  Volume 13, Issue 20

    Abstract: The etiology of feline inflammatory bronchial disease is poorly understood. This study compares the degree of allergen-specific serum IgE responses between cats with feline asthma, chronic bronchitis, mixed inflammation, and clinically healthy cats (HCs). ...

    Abstract The etiology of feline inflammatory bronchial disease is poorly understood. This study compares the degree of allergen-specific serum IgE responses between cats with feline asthma, chronic bronchitis, mixed inflammation, and clinically healthy cats (HCs). The retrospective case-control study used serum from eighteen cats with eosinophilic inflammation (EI), ten with neutrophilic inflammation (NI), six with mixed inflammation (MI), and fourteen HCs. Affected cats were categorized into groups based on bronchoalveolar lavage cytology. The measurement of IgE for 34 different allergens including fungal organisms, weeds, grasses, trees, mites, and insects was performed using an indirect ELISA. Positive reactions to allergens were detected in the serum of 17/18 cats with EI, 8/10 with NI, 6/6 with MI, and 11/14 HCs (
    Language English
    Publishing date 2023-10-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani13203226
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: BTK mutations in patients with chronic lymphocytic leukemia receiving tirabrutinib.

    Jackson, Ross A / Britton, Robert G / Jayne, Sandrine / Lehmann, Susann / Cowley, Caroline M / Trethewey, Christopher S / Smith, Victoria M / Schmid, Ralf / Fegan, Christopher / Walter, Harriet S / Dyer, Martin J S

    Blood advances

    2023  Volume 7, Issue 14, Page(s) 3378–3381

    MeSH term(s) Humans ; Leukemia, Lymphocytic, Chronic, B-Cell/drug therapy ; Leukemia, Lymphocytic, Chronic, B-Cell/genetics ; Mutation ; Pyrimidines/therapeutic use ; Imidazoles
    Chemical Substances tirabrutinib (LXG44NDL2T) ; Pyrimidines ; Imidazoles
    Language English
    Publishing date 2023-05-29
    Publishing country United States
    Document type Research Support, Non-U.S. Gov't ; Letter
    ZDB-ID 2915908-8
    ISSN 2473-9537 ; 2473-9529
    ISSN (online) 2473-9537
    ISSN 2473-9529
    DOI 10.1182/bloodadvances.2022009366
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Fully Flexible Ligand Docking for the P2X7 Receptor Using ROSIE.

    Dayl, Sudad / Schmid, Ralf

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2510, Page(s) 65–75

    Abstract: The availability of P2X7 receptor structures with allosteric antagonists bound enables us to predict specific interactions between receptor and antagonists at atomistic detail. In this chapter we outline how modern ligand docking techniques can be ... ...

    Abstract The availability of P2X7 receptor structures with allosteric antagonists bound enables us to predict specific interactions between receptor and antagonists at atomistic detail. In this chapter we outline how modern ligand docking techniques can be employed by the nonexpert to predict putative binding modes for known or hypothetical allosteric P2X7 antagonists.
    MeSH term(s) Ligands ; Protein Binding ; Receptors, Purinergic P2X7
    Chemical Substances Ligands ; Receptors, Purinergic P2X7
    Language English
    Publishing date 2022-07-01
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2384-8_4
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  4. Article ; Online: Correction to: Chd8 haploinsufficiency impairs early brain development and protein homeostasis later in life.

    Jiménez, Jessica A / Ptacek, Travis S / Tuttle, Alex H / Schmid, Ralf S / Moy, Sheryl S / Simon, Jeremy M / Zylka, Mark J

    Molecular autism

    2021  Volume 12, Issue 1, Page(s) 33

    Language English
    Publishing date 2021-05-08
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2540930-X
    ISSN 2040-2392 ; 2040-2392
    ISSN (online) 2040-2392
    ISSN 2040-2392
    DOI 10.1186/s13229-021-00438-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Limitations of Monitoring Disease Progression Using Circulating Tumor DNA in Lymphoma: An Example From Primary Cutaneous DLBCL Leg-type.

    Trethewey, Christopher S / Walter, Harriet S / Alqahtani, Abdullah N M / Schmid, Ralf / Guttery, David S / Griffin, Yvette / Ahearne, Matthew J / Saldanha, Gerald S / Jayne, Sandrine P N / Dyer, Martin J S

    HemaSphere

    2022  Volume 6, Issue 3, Page(s) e690

    Language English
    Publishing date 2022-03-01
    Publishing country United States
    Document type Case Reports
    ISSN 2572-9241
    ISSN (online) 2572-9241
    DOI 10.1097/HS9.0000000000000690
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: CRISPR/Cas9 directed to the Ube3a antisense transcript improves Angelman syndrome phenotype in mice.

    Schmid, Ralf S / Deng, Xuefeng / Panikker, Priyalakshmi / Msackyi, Msema / Breton, Camilo / Wilson, James M

    The Journal of clinical investigation

    2021  Volume 131, Issue 5

    Abstract: Gene editing holds the potential to correct mutations and cure devastating genetic disorders. The technology has not yet proven efficacious for therapeutic use in CNS diseases with ubiquitous neuronal defects. Angelman syndrome (AS), a severe ... ...

    Abstract Gene editing holds the potential to correct mutations and cure devastating genetic disorders. The technology has not yet proven efficacious for therapeutic use in CNS diseases with ubiquitous neuronal defects. Angelman syndrome (AS), a severe neurodevelopmental disorder, is caused by a lack of maternal expression of the UBE3A gene. Because of genomic imprinting, only neurons are affected. One therapeutic approach focuses on the intact paternal UBE3A copy in patients with AS that is silenced by an antisense transcript (UBE3A-ATS). We show here that gene editing of Ube3a-ATS in the mouse brain resulted in the formation of base pair insertions/deletions (indels) in neurons and the subsequent unsilencing of the paternal Ube3a allele in neurons, which partially corrected the behavioral phenotype of a murine AS model. This study provides compelling evidence to further investigate editing of the homologous region of the human UBE3A-ATS because this may provide a lasting therapeutic effect for patients with AS.
    Language English
    Publishing date 2021-01-08
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3067-3
    ISSN 1558-8238 ; 0021-9738
    ISSN (online) 1558-8238
    ISSN 0021-9738
    DOI 10.1172/JCI142574
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: A Coupled Mathematical Model of the Intracellular Replication of Dengue Virus and the Host Cell Immune Response to Infection.

    Zitzmann, Carolin / Schmid, Bianca / Ruggieri, Alessia / Perelson, Alan S / Binder, Marco / Bartenschlager, Ralf / Kaderali, Lars

    Frontiers in microbiology

    2020  Volume 11, Page(s) 725

    Abstract: Dengue virus (DV) is a positive-strand RNA virus of ... ...

    Abstract Dengue virus (DV) is a positive-strand RNA virus of the
    Language English
    Publishing date 2020-04-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2020.00725
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Mathematical modeling of plus-strand RNA virus replication to identify broad-spectrum antiviral treatment strategies.

    Zitzmann, Carolin / Dächert, Christopher / Schmid, Bianca / van der Schaar, Hilde / van Hemert, Martijn / Perelson, Alan S / van Kuppeveld, Frank J M / Bartenschlager, Ralf / Binder, Marco / Kaderali, Lars

    PLoS computational biology

    2023  Volume 19, Issue 4, Page(s) e1010423

    Abstract: Plus-strand RNA viruses are the largest group of viruses. Many are human pathogens that inflict a socio-economic burden. Interestingly, plus-strand RNA viruses share remarkable similarities in their replication. A hallmark of plus-strand RNA viruses is ... ...

    Abstract Plus-strand RNA viruses are the largest group of viruses. Many are human pathogens that inflict a socio-economic burden. Interestingly, plus-strand RNA viruses share remarkable similarities in their replication. A hallmark of plus-strand RNA viruses is the remodeling of intracellular membranes to establish replication organelles (so-called "replication factories"), which provide a protected environment for the replicase complex, consisting of the viral genome and proteins necessary for viral RNA synthesis. In the current study, we investigate pan-viral similarities and virus-specific differences in the life cycle of this highly relevant group of viruses. We first measured the kinetics of viral RNA, viral protein, and infectious virus particle production of hepatitis C virus (HCV), dengue virus (DENV), and coxsackievirus B3 (CVB3) in the immuno-compromised Huh7 cell line and thus without perturbations by an intrinsic immune response. Based on these measurements, we developed a detailed mathematical model of the replication of HCV, DENV, and CVB3 and showed that only small virus-specific changes in the model were necessary to describe the in vitro dynamics of the different viruses. Our model correctly predicted virus-specific mechanisms such as host cell translation shut off and different kinetics of replication organelles. Further, our model suggests that the ability to suppress or shut down host cell mRNA translation may be a key factor for in vitro replication efficiency, which may determine acute self-limited or chronic infection. We further analyzed potential broad-spectrum antiviral treatment options in silico and found that targeting viral RNA translation, such as polyprotein cleavage and viral RNA synthesis, may be the most promising drug targets for all plus-strand RNA viruses. Moreover, we found that targeting only the formation of replicase complexes did not stop the in vitro viral replication early in infection, while inhibiting intracellular trafficking processes may even lead to amplified viral growth.
    MeSH term(s) Humans ; Antiviral Agents/pharmacology ; RNA Viruses ; Virus Replication/physiology ; RNA, Viral/genetics ; Models, Theoretical ; Hepatitis C
    Chemical Substances Antiviral Agents ; RNA, Viral
    Language English
    Publishing date 2023-04-04
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2193340-6
    ISSN 1553-7358 ; 1553-734X
    ISSN (online) 1553-7358
    ISSN 1553-734X
    DOI 10.1371/journal.pcbi.1010423
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Mathematical modeling of plus-strand RNA virus replication to identify broad-spectrum antiviral treatment strategies.

    Carolin Zitzmann / Christopher Dächert / Bianca Schmid / Hilde van der Schaar / Martijn van Hemert / Alan S Perelson / Frank J M van Kuppeveld / Ralf Bartenschlager / Marco Binder / Lars Kaderali

    PLoS Computational Biology, Vol 19, Iss 4, p e

    2023  Volume 1010423

    Abstract: Plus-strand RNA viruses are the largest group of viruses. Many are human pathogens that inflict a socio-economic burden. Interestingly, plus-strand RNA viruses share remarkable similarities in their replication. A hallmark of plus-strand RNA viruses is ... ...

    Abstract Plus-strand RNA viruses are the largest group of viruses. Many are human pathogens that inflict a socio-economic burden. Interestingly, plus-strand RNA viruses share remarkable similarities in their replication. A hallmark of plus-strand RNA viruses is the remodeling of intracellular membranes to establish replication organelles (so-called "replication factories"), which provide a protected environment for the replicase complex, consisting of the viral genome and proteins necessary for viral RNA synthesis. In the current study, we investigate pan-viral similarities and virus-specific differences in the life cycle of this highly relevant group of viruses. We first measured the kinetics of viral RNA, viral protein, and infectious virus particle production of hepatitis C virus (HCV), dengue virus (DENV), and coxsackievirus B3 (CVB3) in the immuno-compromised Huh7 cell line and thus without perturbations by an intrinsic immune response. Based on these measurements, we developed a detailed mathematical model of the replication of HCV, DENV, and CVB3 and showed that only small virus-specific changes in the model were necessary to describe the in vitro dynamics of the different viruses. Our model correctly predicted virus-specific mechanisms such as host cell translation shut off and different kinetics of replication organelles. Further, our model suggests that the ability to suppress or shut down host cell mRNA translation may be a key factor for in vitro replication efficiency, which may determine acute self-limited or chronic infection. We further analyzed potential broad-spectrum antiviral treatment options in silico and found that targeting viral RNA translation, such as polyprotein cleavage and viral RNA synthesis, may be the most promising drug targets for all plus-strand RNA viruses. Moreover, we found that targeting only the formation of replicase complexes did not stop the in vitro viral replication early in infection, while inhibiting intracellular trafficking processes may even lead to amplified viral ...
    Keywords Biology (General) ; QH301-705.5
    Subject code 570
    Language English
    Publishing date 2023-04-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Survival and hormone production of isolated mouse follicles in three-dimensional artificial scaffolds after stimulation with bpV(HOpic).

    Keckstein, Philip / Dittrich, Ralf / Bleisinger, Nathalie / Hoffmann, Inge / Beckmann, Matthias W / Gebhardt, Albrecht / Schmid, Benjamin / Keckstein, Simon

    Archives of gynecology and obstetrics

    2024  Volume 309, Issue 5, Page(s) 2127–2136

    Abstract: Purpose: To preserve fertility before gonadotoxic therapy, ovarian tissue can be removed, cryopreserved, and transplanted back again after treatment. An alternative is the artificial ovary, in which the ovarian follicles are extracted from the tissue, ... ...

    Abstract Purpose: To preserve fertility before gonadotoxic therapy, ovarian tissue can be removed, cryopreserved, and transplanted back again after treatment. An alternative is the artificial ovary, in which the ovarian follicles are extracted from the tissue, which reduces the risk of reimplantation of potentially remaining malignant cells. The PTEN inhibitor bpV(HOpic) has been shown to activate human, bovine and alpacas ovarian follicles, and it is therefore considered a promising substance for developing the artificial ovary. The purpose of this study was to examine the impact of different scaffolds and the vanadate derivative bpV(HOpic) on mice follicle survival and hormone secretion over 10 days.
    Methods: A comparative analysis was performed, studying the survival rates (SR) of isolated mice follicle in four different groups that differed either in the scaffold (polycaprolactone scaffold versus polyethylene terephthalate membrane) or in the medium-bpV(HOpic) versus control medium. The observation period of the follicles was 10 days. On days 2, 6, and 10, the viability and morphology of the follicles were checked using fluorescence or confocal microscopy. Furthermore, hormone levels of estrogen (pmol/L) and progesterone (nmol/L) were determined.
    Results: When comparing the SR of follicles among the four groups, it was observed that on day 6, the study groups utilizing the polycaprolactone scaffold with bpV(HOpic) in the medium (SR: 0.48 ± 0.18; p = 0.004) or functionalized in the scaffold (SR: 0.50 ± 0.20; p = 0.003) exhibited significantly higher survival rates compared to the group using only the polyethylene terephthalate membrane (SR: 0). On day 10, a significantly higher survival rate was only noted when comparing the polycaprolactone scaffold with bpV(HOpic) in the medium to the polyethylene terephthalate membrane group (SR: 0.38 ± 0.20 versus 0; p = 0.007). Higher levels of progesterone were only significantly associated with better survival rates in the group with the polycaprolactone scaffold functionalized with bpV(HOpic) (p = 0.017).
    Conclusion: This study demonstrates that three-dimensional polycaprolactone scaffolds improve the survival rates of isolated mice follicles in comparison with a conventional polyethylene terephthalate membrane. The survival rates slightly improve with added bpV(HOpic). Furthermore, higher rates of progesterone were also partly associated with improved survival.
    MeSH term(s) Female ; Mice ; Animals ; Humans ; Cattle ; Progesterone/pharmacology ; Polyethylene Terephthalates ; Ovarian Follicle/physiology ; Ovary ; Cryopreservation
    Chemical Substances Progesterone (4G7DS2Q64Y) ; Polyethylene Terephthalates
    Language English
    Publishing date 2024-03-12
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 896455-5
    ISSN 1432-0711 ; 0932-0067
    ISSN (online) 1432-0711
    ISSN 0932-0067
    DOI 10.1007/s00404-024-07419-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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