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  1. Article: Plants in traditional medicine with special reference to

    Kamala, Arunagiri / Middha, Sushil Kumar / Karigar, Chandrakant S

    3 Biotech

    2018  Volume 8, Issue 7, Page(s) 309

    Abstract: The nutgrass, ...

    Abstract The nutgrass,
    Language English
    Publishing date 2018-07-09
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 2600522-0
    ISSN 2190-5738 ; 2190-572X
    ISSN (online) 2190-5738
    ISSN 2190-572X
    DOI 10.1007/s13205-018-1328-6
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  2. Article: Plants in traditional medicine with special reference to Cyperus rotundus L.: a review

    Kamala, Arunagiri / Middha, Sushil Kumar / Karigar, Chandrakant S

    3 Biotech. 2018 July, v. 8, no. 7

    2018  

    Abstract: The nutgrass, Cyperus rotundus L. (Family: Cyperaceae), is a colonial, perennial herb considered to have originated in India 2000 years ago and widely used in Ayurveda to treat several ailments. In addition to its prehistoric uses, it is used in several ... ...

    Abstract The nutgrass, Cyperus rotundus L. (Family: Cyperaceae), is a colonial, perennial herb considered to have originated in India 2000 years ago and widely used in Ayurveda to treat several ailments. In addition to its prehistoric uses, it is used in several systems of medicine for treating variety of diseases. The synergistic actions of the Cyperus’ compounds have added advantage over that of a single constituent. In the past decade, numerous studies proved analgesic, anti-allergic, anti-arthritic, anti-candida, anti-cariogenic, anti-convulsant, anti-diarrheal, anti-emetic, anti-helminthic, anti-histamine, anti-hyperglycemic, anti-hypertensive, anti-inflammatory, anti-malarial, anti-obesity, antioxidant, anti-platelet, anti-pyretic, anti-ulcer, anti-viral, cardioprotective, cytoprotective, cytotoxic, gastroprotective, hepatoprotective, neuroprotective, ovicidal, and larvicidal, wound healing and inhibition of brain Na+ K+ ATPase activities of C. rotundus and its chemical constituents. However, the exact the mechanism of action is not very clear and requires further evaluation. These properties strongly suggest an extensive use of C. rotundus for clinical applications. In this review, we attempted to provide information about the pharmacological effects of Cyperus and its proposed mechanisms of actions.
    Keywords Ayurvedic medicine ; Cyperus rotundus ; adenosinetriphosphatase ; analgesics ; anthelmintics ; anti-allergic agents ; antimalarials ; antioxidants ; brain ; chemical composition ; cytotoxicity ; enzyme activity ; glycemic effect ; larvicides ; mechanism of action ; medicinal plants ; potassium ; sodium ; India
    Language English
    Dates of publication 2018-07
    Size p. 309.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    Note Review
    ZDB-ID 2600522-0
    ISSN 2190-5738 ; 2190-572X
    ISSN (online) 2190-5738
    ISSN 2190-572X
    DOI 10.1007/s13205-018-1328-6
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  3. Article ; Online: Role of microbial enzymes in the bioremediation of pollutants: a review.

    Karigar, Chandrakant S / Rao, Shwetha S

    Enzyme research

    2011  Volume 2011, Page(s) 805187

    Abstract: A large number of enzymes from bacteria, fungi, and plants have been reported to be involved in the biodegradation of toxic organic pollutants. Bioremediation is a cost effective and nature friendly biotechnology that is powered by microbial enzymes. The ...

    Abstract A large number of enzymes from bacteria, fungi, and plants have been reported to be involved in the biodegradation of toxic organic pollutants. Bioremediation is a cost effective and nature friendly biotechnology that is powered by microbial enzymes. The research activity in this area would contribute towards developing advanced bioprocess technology to reduce the toxicity of the pollutants and also to obtain novel useful substances. The information on the mechanisms of bioremediation-related enzymes such as oxido-reductases and hydrolases have been extensively studied. This review attempts to provide descriptive information on the enzymes from various microorganisms involved in the biodegradation of wide range of pollutants, applications, and suggestions required to overcome the limitations of their efficient use.
    Language English
    Publishing date 2011-09-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2573712-0
    ISSN 2090-0414 ; 2090-0406
    ISSN (online) 2090-0414
    ISSN 2090-0406
    DOI 10.4061/2011/805187
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  4. Article: In vitro

    Kamala, Arunagiri / Middha, Sushil Kumar / Gopinath, Chitra / Sindhura, H S / Karigar, Chandrakant S

    publication RETRACTED

    Pharmacognosy magazine

    2018  Volume 14, Issue 54, Page(s) 261–267

    Abstract: Background: Cyperus rotundus: Objective: The objective of the study was to optimize the best solvent system and bioprospect the possible phytochemicals in : Materials and methods: The phytochemicals were extracted from the rhizomes of : Results ... ...

    Abstract Background: Cyperus rotundus
    Objective: The objective of the study was to optimize the best solvent system and bioprospect the possible phytochemicals in
    Materials and methods: The phytochemicals were extracted from the rhizomes of
    Results and discussion: The TPC, TFC, and TPAC estimates ranged from 0.036 ± 0.002 to 118.924 ± 5.946 μg/mg extract, 7.196 ± 0.359 to 200.654 ± 10.032 μg/mg extract, and 13.115 ± 0.656 to 45.901 ± 2.295 μg/mg extract, respectively. The quantities of TPC, TFC, and TPAC were found to be the highest in 70% acetone extract. The 70% acetone and 70% methanol extracts revealed best radical scavenging effect. GC-MS analysis of CRR extract revealed the presence of a novel compound 1 (2)-acetyl-3 (5)-styryl-5 (3)-methylthiopyrazole.
    Conclusion: The study indicated that 70% acetone and 70% methanol extracts of CRRs can be a potential source of antioxidants.
    Summary: The studies suggest 70% methanol and acetone as the suitable solvents for the extraction of phytochemicalsNovel compound 1(2)-Acetyl-3(5)-styryl-5(3)-methylthiopyrazole was detected in 70% acetone extract.
    Language English
    Publishing date 2018-04-10
    Publishing country United States
    Document type Journal Article ; Retracted Publication
    ZDB-ID 2274976-7
    ISSN 0976-4062 ; 0973-1296
    ISSN (online) 0976-4062
    ISSN 0973-1296
    DOI 10.4103/pm.pm_228_17
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Role of Microbial Enzymes in the Bioremediation of Pollutants

    Chandrakant S. Karigar / Shwetha S. Rao

    Enzyme Research, Vol

    A Review

    2011  Volume 2011

    Abstract: A large number of enzymes from bacteria, fungi, and plants have been reported to be involved in the biodegradation of toxic organic pollutants. Bioremediation is a cost effective and nature friendly biotechnology that is powered by microbial enzymes. The ...

    Abstract A large number of enzymes from bacteria, fungi, and plants have been reported to be involved in the biodegradation of toxic organic pollutants. Bioremediation is a cost effective and nature friendly biotechnology that is powered by microbial enzymes. The research activity in this area would contribute towards developing advanced bioprocess technology to reduce the toxicity of the pollutants and also to obtain novel useful substances. The information on the mechanisms of bioremediation-related enzymes such as oxido-reductases and hydrolases have been extensively studied. This review attempts to provide descriptive information on the enzymes from various microorganisms involved in the biodegradation of wide range of pollutants, applications, and suggestions required to overcome the limitations of their efficient use.
    Keywords Biology (General) ; QH301-705.5 ; Biochemistry ; QD415-436
    Language English
    Publishing date 2011-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Production of tropane alkaloids during de-differentiation of Scopolia parviflora calli.

    Kim, Yong Duck / Kang, Seung Mi / Min, Ji Yun / Choi, Won Kyun / Jeong, Mi Jin / Karigar, Chandrakant S / Choi, Myung Suk

    Journal of natural products

    2010  Volume 73, Issue 2, Page(s) 147–150

    Abstract: The production of tropane alkaloids during differentiation and de-differentiation of Scopolia parviflora calli was studied. Tropane alkaloid production drastically decreased during calli de-differentiation. Scopolamine (1) production decreased after 10 ... ...

    Abstract The production of tropane alkaloids during differentiation and de-differentiation of Scopolia parviflora calli was studied. Tropane alkaloid production drastically decreased during calli de-differentiation. Scopolamine (1) production decreased after 10 days of culture, whereas that of hyoscyamine (2) decreased during de-differentiation of root to calli. The production of 1 was enhanced in calli undergoing differentiation to shoot after 60 days of culture, reaching a maximum by 80 days. However, production of hyoscyamine in regenerated plants was lower. The expression level of hyoscyamine 6beta-hydroxylase (H6H), a key biosynthetic enzyme for tropane alkaloids, was significantly increased in 4-week-old calli. This study suggests that the biosynthesis of tropane alkaloids is regulated inversely in de-differentiating Scopolia parviflora calli.
    MeSH term(s) Korea ; Molecular Structure ; Plant Roots/chemistry ; Scopolamine Hydrobromide/biosynthesis ; Scopolia/chemistry ; Scopolia/growth & development ; Tropanes/chemistry ; Tropanes/isolation & purification
    Chemical Substances Tropanes ; Scopolamine Hydrobromide (451IFR0GXB)
    Language English
    Publishing date 2010-02-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 304325-3
    ISSN 1520-6025 ; 0163-3864
    ISSN (online) 1520-6025
    ISSN 0163-3864
    DOI 10.1021/np900525n
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1144: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  7. Article: Degradation of ethylbenzene by free and immobilized Pseudomonas fluorescens-CS2.

    Parameswarappa, Suneetha / Karigar, Chandrakant / Nagenahalli, Manjunath

    Biodegradation

    2008  Volume 19, Issue 1, Page(s) 137–144

    Abstract: Pseudomonas fluorescens-CS2 metabolized ethylbenzene as the sole source of carbon and energy. The involvement of catechol as the hydroxylated intermediate during the biodegradation of ethylbenzene was established by TLC, HPLC and enzyme analysis. The ... ...

    Abstract Pseudomonas fluorescens-CS2 metabolized ethylbenzene as the sole source of carbon and energy. The involvement of catechol as the hydroxylated intermediate during the biodegradation of ethylbenzene was established by TLC, HPLC and enzyme analysis. The specific activity of Catechol 2,3-dioxygenase in the cell free extracts of P. fluorescens-CS2 was determined to be 0.428 micromoles min(-1) mg(-1) protein. An aqueous-organic, Two-Phase Batch Culture System (TPBCS) was developed to overcome inhibition due to higher substrate concentrations. In TPBCS, P. fluorescens-CS2 demonstrated ethylbenzene utilization up to 50 mM without substrate inhibition on inclusion of n-decanol as the second phase. The rate of ethylbenzene metabolism in TPBCS was found enhance by fivefold in comparison with single phase system. Alternatively the alginate, agar and polyacrylamide matrix immobilized P. fluorescens-CS2 cells efficiently degraded ethylebenzene with enhanced efficiency compared to free cell cultures in single and two-phase systems. The cells entrapped in ployacrylamide and alginate were found to be stable and degradation efficient for a period of 42 days where as agar-entrapped P. fluorescens was stable and efficient a period of 36 days. This demonstrates that alginate and polyacrylamide matrices are more promising as compared to agar for cell immobilization.
    MeSH term(s) Benzene Derivatives/metabolism ; Biodegradation, Environmental ; Catechols/metabolism ; Cells, Immobilized/metabolism ; Pseudomonas fluorescens/metabolism
    Chemical Substances Benzene Derivatives ; Catechols ; ethylbenzene (L5I45M5G0O) ; catechol (LF3AJ089DQ)
    Language English
    Publishing date 2008-02
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1056014-2
    ISSN 1572-9729 ; 0923-9820
    ISSN (online) 1572-9729
    ISSN 0923-9820
    DOI 10.1007/s10532-007-9121-y
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    Zs.A 3094: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  8. Article ; Audio / Video: Production of Tropane Alkaloids during De-differentiation of Scopolia parviflora Calli

    Kim, Yong Duck / Kang, Seung Mi / Min, Ji Yun / Choi, Won Kyun / Jeong, Mi Jin / Karigar, Chandrakant. S / Choi, Myung Suk

    Journal of natural products. 2010 Feb. 26, v. 73, no. 2

    2010  

    Language English
    Dates of publication 2010-0226
    Size p. 147–150.
    Document type Article ; Audio / Video
    ZDB-ID 304325-3
    ISSN 1520-6025 ; 0163-3864
    ISSN (online) 1520-6025
    ISSN 0163-3864
    DOI 10.1021/np900525n
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1144: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article: Rapid selection of catechin-rich tea trees (Camellia sinensis) by a colorimetric method

    Kim, Yong Duck / Chandrakant S. Karigar / Gang Won Cheong / Hyun Jin Song / Ji Yun Min / Jung Gyu Hwang / Mi Jin Jeong / Myung Suk Choi

    Journal of wood science. 2010 Oct., v. 56, no. 5

    2010  

    Abstract: A rapid and efficient colorimetric method based on the use of Fast Blue B-salt (FBB) was established to select catechin-rich tea trees (Camellia sinensis L.). The catechin levels measured by the colorimetric method under optimized reaction conditions ... ...

    Abstract A rapid and efficient colorimetric method based on the use of Fast Blue B-salt (FBB) was established to select catechin-rich tea trees (Camellia sinensis L.). The catechin levels measured by the colorimetric method under optimized reaction conditions correlated closely with estimations by high-performance liquid chromatography (HPLC) analysis. The FBB colorimetric method was successfully used to classify 160 tea trees on the basis of their catechin contents into rich and poor lines. HPLC analysis of the FBB-selected tea tree extracts showed them to contain (−)-epigallocatechin 186 mg/g in tea tree line HR-29, (−)-epicatechin 43.7 mg/g in HR-82, (−)-epigallocatechin gallate 4.32 mg/g in HR-29, and (−)-epicatechin gallate 0.22 mg/g in HR-52. Classification of tea trees from the Hadong region into catechin-rich and -poor trees was independent of the growing season. Thus the FBB colorimetric method could find application as a reliable tool in screening and selection of tea trees on the basis of their catechin content.
    Keywords Camellia sinensis ; catechin ; colorimetry ; growing season ; high performance liquid chromatography ; screening ; tea ; treeline ; trees
    Language English
    Dates of publication 2010-10
    Size p. 411-417.
    Publishing place Springer Japan
    Document type Article
    ZDB-ID 1429032-7
    ISSN 1435-0211
    ISSN 1435-0211
    DOI 10.1007/s10086-010-1112-7
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    In stock of ZB MED Bonn / Germany

    Z 6929: Show issues

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  10. Article: Degradation of ethylbenzene by free and immobilized Pseudomonas fluorescens-CS2

    Parameswarappa, Suneetha / Karigar, Chandrakant / Nagenahalli, Manjunath

    Biodegradation. 2008 Feb., v. 19, no. 1

    2008  

    Abstract: Pseudomonas fluorescens-CS2 metabolized ethylbenzene as the sole source of carbon and energy. The involvement of catechol as the hydroxylated intermediate during the biodegradation of ethylbenzene was established by TLC, HPLC and enzyme analysis. The ... ...

    Abstract Pseudomonas fluorescens-CS2 metabolized ethylbenzene as the sole source of carbon and energy. The involvement of catechol as the hydroxylated intermediate during the biodegradation of ethylbenzene was established by TLC, HPLC and enzyme analysis. The specific activity of Catechol 2,3-dioxygenase in the cell free extracts of P. fluorescens-CS2 was determined to be 0.428 μmoles min-¹ mg-¹ protein. An aqueous-organic, Two-Phase Batch Culture System (TPBCS) was developed to overcome inhibition due to higher substrate concentrations. In TPBCS, P. fluorescens-CS2 demonstrated ethylbenzene utilization up to 50 mM without substrate inhibition on inclusion of n-decanol as the second phase. The rate of ethylbenzene metabolism in TPBCS was found enhance by fivefold in comparison with single phase system. Alternatively the alginate, agar and polyacrylamide matrix immobilized P. fluorescens-CS2 cells efficiently degraded ethylebenzene with enhanced efficiency compared to free cell cultures in single and two-phase systems. The cells entrapped in ployacrylamide and alginate were found to be stable and degradation efficient for a period of 42 days where as agar-entrapped P. fluorescens was stable and efficient a period of 36 days. This demonstrates that alginate and polyacrylamide matrices are more promising as compared to agar for cell immobilization.
    Keywords Pseudomonas fluorescens ; biodegradation ; catechol
    Language English
    Dates of publication 2008-02
    Size p. 137-144.
    Publisher Springer Netherlands
    Publishing place Dordrecht
    Document type Article
    ZDB-ID 1056014-2
    ISSN 1572-9729 ; 0923-9820
    ISSN (online) 1572-9729
    ISSN 0923-9820
    DOI 10.1007/s10532-007-9121-y
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    In stock of ZB MED Bonn / Germany

    Z 5897: Show issues

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