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Article ; Online: Correction to: Genetically engineered probiotic Saccharomyces cerevisiae strains mature human dendritic cells and stimulate gag-specific memory CD8

Palma, Mariana L / Garcia-Bates, Tatiana M / Martins, Flaviano S / Douradinha, Bruno

2019 Volume 103, Issue 13, Page(s) 5461

Abstract: In the Funding section, the following statement is missing: The MACS cohort study was supported by the NIH, National Institute of Allergy and Infectious Diseases grant U01-AI35041. ...

Abstract	In the Funding section, the following statement is missing: The MACS cohort study was supported by the NIH, National Institute of Allergy and Infectious Diseases grant U01-AI35041.
Language	English
Publishing date	2019-03-22
Publishing country	Germany
Document type	Journal Article ; Published Erratum
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-019-09911-y
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Article ; Online: Genetically engineered probiotic Saccharomyces cerevisiae strains mature human dendritic cells and stimulate Gag-specific memory CD8

Palma, Mariana L / Garcia-Bates, Tatiana M / Martins, Flaviano S / Douradinha, Bruno

Applied microbiology and biotechnology

2019 Volume 103, Issue 13, Page(s) 5183–5192

Abstract: Recombinant Saccharomyces cerevisiae strains expressing HIV antigens have shown promising pre-clinical results. Probiotic S. cerevisiae strains naturally induce gut immunity; thus, genetically engineered probiotic strains could be used to stimulate ... ...

Abstract	Recombinant Saccharomyces cerevisiae strains expressing HIV antigens have shown promising pre-clinical results. Probiotic S. cerevisiae strains naturally induce gut immunity; thus, genetically engineered probiotic strains could be used to stimulate immune responses against HIV in the mucosa. Probiotic strains have a higher rate of heterologous protein production, meaning higher antigen's epitope expression levels per yeast cell. We expressed HIV-1 Gag protein in the probiotic yeasts' surface, which was eagerly phagocytosed by and induced type 1 polarization of human monocyte-derived dendritic cells (DCs) from healthy donors in vitro. We further matured DCs derived from HIV-1
MeSH term(s)	AIDS Vaccines/immunology ; Antigen Presentation ; CD8-Positive T-Lymphocytes/immunology ; Cell Differentiation ; Dendritic Cells/immunology ; Genetic Engineering ; HIV-1/genetics ; HIV-1/immunology ; Humans ; Immunologic Memory ; Male ; Phagocytosis ; Probiotics ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/immunology ; Th1 Cells/immunology ; gag Gene Products, Human Immunodeficiency Virus/genetics ; gag Gene Products, Human Immunodeficiency Virus/immunology
Chemical Substances	AIDS Vaccines ; gag Gene Products, Human Immunodeficiency Virus
Language	English
Publishing date	2019-04-24
Publishing country	Germany
Document type	Journal Article
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-019-09842-8
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Article: Correction to: Genetically engineered probiotic Saccharomyces cerevisiae strains mature human dendritic cells and stimulate gag-specific memory CD8+ T cells ex vivo

Palma, Mariana L / Douradinha, Bruno / Garcia-Bates, Tatiana M / Martins, Flaviano S

Applied microbiology and biotechnology. 2019 July, v. 103, no. 13

2019

Abstract: In the Funding section, the following statement is missing: The MACS cohort study was supported by the NIH, National Institute of Allergy and Infectious Diseases grant U01-AI35041. ...

Abstract	In the Funding section, the following statement is missing: The MACS cohort study was supported by the NIH, National Institute of Allergy and Infectious Diseases grant U01-AI35041.
Keywords	CD8-positive T-lymphocytes ; cohort studies ; dendritic cells ; genetic engineering ; humans ; probiotics ; Saccharomyces cerevisiae
Language	English
Dates of publication	2019-07
Size	p. 5461.
Publishing place	Springer Berlin Heidelberg
Document type	Article
Note	Published Erratum
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-019-09911-y
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Article ; Online: Type 1-programmed dendritic cells drive antigen-specific latency reversal and immune elimination of persistent HIV-1.

Kristoff, Jan / Palma, Mariana L / Garcia-Bates, Tatiana M / Shen, Chengli / Sluis-Cremer, Nicolas / Gupta, Phalguni / Rinaldo, Charles R / Mailliard, Robbie B

EBioMedicine

2019 Volume 43, Page(s) 295–306

Abstract: Background: Despite the success of antiretroviral therapy (ART), latent HIV-1 continues to persist in a long-lived population of resting memory CD4: Methods: In this study, we test the use of antigen-presenting type 1-polarized, monocyte-derived ... ...

Abstract	Background: Despite the success of antiretroviral therapy (ART), latent HIV-1 continues to persist in a long-lived population of resting memory CD4 Methods: In this study, we test the use of antigen-presenting type 1-polarized, monocyte-derived dendritic cells (MDC1) generated from chronic HIV-1-infected individuals on ART as a means to induce HIV-1 latency reversal in autologous CD4 Findings: MDC1 presentation of either HIV-1 or cytomegalovirus (CMV) antigens to CD4 Interpretation: Inclusion of virus-associated MHC class II 'helper' antigens in MDC1-based HIV-1 immunotherapies could serve both as a targeted means to safely unmask antigen-specific CD4
MeSH term(s)	Antigens, Viral ; Biomarkers ; CD4-Positive T-Lymphocytes/immunology ; CD4-Positive T-Lymphocytes/metabolism ; CD4-Positive T-Lymphocytes/virology ; CD8-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/metabolism ; Dendritic Cells/immunology ; Dendritic Cells/metabolism ; Epitopes/immunology ; HIV Infections/drug therapy ; HIV Infections/immunology ; HIV Infections/metabolism ; HIV Infections/virology ; HIV-1/physiology ; Host-Pathogen Interactions/immunology ; Humans ; Interferon-gamma/metabolism ; RNA, Viral ; T-Lymphocytes, Cytotoxic/immunology ; Virus Latency/immunology ; Virus Replication
Chemical Substances	Antigens, Viral ; Biomarkers ; Epitopes ; RNA, Viral ; Interferon-gamma (82115-62-6)
Language	English
Publishing date	2019-04-02
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2851331-9
ISSN	2352-3964
ISSN (online)	2352-3964
DOI	10.1016/j.ebiom.2019.03.077
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Article: Genetically engineered probiotic Saccharomyces cerevisiae strains mature human dendritic cells and stimulate Gag-specific memory CD8+ T cells ex vivo

Palma, Mariana L / Douradinha, Bruno / Garcia-Bates, Tatiana M / Martins, Flaviano S

Applied microbiology and biotechnology. 2019 July, v. 103, no. 13

2019

Abstract	Recombinant Saccharomyces cerevisiae strains expressing HIV antigens have shown promising pre-clinical results. Probiotic S. cerevisiae strains naturally induce gut immunity; thus, genetically engineered probiotic strains could be used to stimulate immune responses against HIV in the mucosa. Probiotic strains have a higher rate of heterologous protein production, meaning higher antigen’s epitope expression levels per yeast cell. We expressed HIV-1 Gag protein in the probiotic yeasts’ surface, which was eagerly phagocytosed by and induced type 1 polarization of human monocyte-derived dendritic cells (DCs) from healthy donors in vitro. We further matured DCs derived from HIV-1+ donors with transformed yeasts and incubated them with autologous T cells. Only DCs matured with Gag-expressing probiotic strains were able to efficiently present antigen to CD8+ T cells and induced their clonal expansion. Our results show that genetically engineered probiotic S. cerevisiae strains are a promising vaccination strategy against HIV.
Keywords	CD8-positive T-lymphocytes ; dendritic cells ; digestive system ; epitopes ; genetic engineering ; heterologous gene expression ; Human immunodeficiency virus 1 ; humans ; immune response ; mucosa ; probiotics ; Saccharomyces cerevisiae ; vaccination ; yeasts
Language	English
Dates of publication	2019-07
Size	p. 5183-5192.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	392453-1
ISSN	1432-0614 ; 0171-1741 ; 0175-7598
ISSN (online)	1432-0614
ISSN	0171-1741 ; 0175-7598
DOI	10.1007/s00253-019-09842-8
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Contrasting Roles of the PD-1 Signaling Pathway in Dendritic Cell-Mediated Induction and Regulation of HIV-1-Specific Effector T Cell Functions.

Garcia-Bates, Tatiana M / Palma, Mariana L / Shen, Chengli / Gambotto, Andrea / Macatangay, Bernard J C / Ferris, Robert L / Rinaldo, Charles R / Mailliard, Robbie B

Journal of virology

2019 Volume 93, Issue 5

Abstract: Eliciting highly functional ... ...

Abstract	Eliciting highly functional CD8
MeSH term(s)	Adult ; B7-H1 Antigen/metabolism ; CD40 Ligand/metabolism ; Dendritic Cells/immunology ; HIV Infections/immunology ; HIV-1/immunology ; Humans ; Immune Evasion/immunology ; Immunologic Memory/immunology ; Interleukin-12 Subunit p35/immunology ; Lymphocyte Activation/immunology ; Programmed Cell Death 1 Receptor/antagonists & inhibitors ; Programmed Cell Death 1 Receptor/metabolism ; Signal Transduction/immunology ; T-Lymphocytes, Cytotoxic/immunology ; T-Lymphocytes, Helper-Inducer/immunology
Chemical Substances	B7-H1 Antigen ; CD274 protein, human ; IL12A protein, human ; Interleukin-12 Subunit p35 ; PDCD1 protein, human ; Programmed Cell Death 1 Receptor ; CD40 Ligand (147205-72-9)
Language	English
Publishing date	2019-02-19
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	80174-4
ISSN	1098-5514 ; 0022-538X
ISSN (online)	1098-5514
ISSN	0022-538X
DOI	10.1128/JVI.02035-18
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Article ; Online: Dendritic cells focus CTL responses toward highly conserved and topologically important HIV-1 epitopes.

Garcia-Bates, Tatiana M / Palma, Mariana L / Anderko, Renee R / Hsu, Denise C / Ananworanich, Jintanat / Korber, Bette T / Gaiha, Gaurav D / Phanuphak, Nittaya / Thomas, Rasmi / Tovanabutra, Sodsai / Walker, Bruce D / Mellors, John W / Piazza, Paolo A / Kroon, Eugene / Riddler, Sharon A / Michael, Nelson L / Rinaldo, Charles R / Mailliard, Robbie B

EBioMedicine

2021 Volume 63, Page(s) 103175

Abstract: Background: During early HIV-1 infection, immunodominant T cell responses to highly variable epitopes lead to the establishment of immune escape virus variants. Here we assessed a type 1-polarized monocyte-derived dendritic cell (MDC1)-based approach to ...

Abstract	Background: During early HIV-1 infection, immunodominant T cell responses to highly variable epitopes lead to the establishment of immune escape virus variants. Here we assessed a type 1-polarized monocyte-derived dendritic cell (MDC1)-based approach to selectively elicit cytotoxic T lymphocyte (CTL) responses against highly conserved and topologically important HIV-1 epitopes in HIV-1-infected individuals from the Thailand RV254/SEARCH 010 cohort who initiated antiretroviral therapy (ART) during early infection (Fiebig stages I-IV). Methods: Autologous MDC1 were used as antigen presenting cells to induce in vitro CTL responses against HIV-1 Gag, Pol, Env, and Nef as determined by flow cytometry and ELISpot assay. Ultra-conserved or topologically important antigens were respectively identified using the Epigraph tool and a structure-based network analysis approach and compared to overlapping peptides spanning the Gag proteome. Findings: MDC1 presenting either the overlapping Gag, Epigraph, or Network 14-21mer peptide pools consistently activated and expanded HIV-1-specific T cells to epitopes identified at the 9-13mer peptide level. Interestingly, some CTL responses occurred outside known or expected HLA associations, providing evidence of new HLA-associated CTL epitopes. Comparative analyses demonstrated more sequence conservation among Epigraph antigens but a higher magnitude of CTL responses to Network and Gag peptide groups. Importantly, CTL responses against topologically constrained Gag epitopes contained in both the Network and Gag peptide pools were selectively enhanced in the Network pool-initiated cultures. Interpretation: Our study supports the use of MDC1 as a therapeutic strategy to induce and focus CTL responses toward putative fitness-constrained regions of HIV-1 to prevent immune escape and control HIV-1 infection. Funding: A full list of the funding sources is detailed in the Acknowledgment section of the manuscript.
MeSH term(s)	Adult ; Alleles ; Amino Acid Sequence ; CD4 Lymphocyte Count ; CD4-CD8 Ratio ; Conserved Sequence ; Dendritic Cells/immunology ; Dendritic Cells/metabolism ; Epitopes, T-Lymphocyte/chemistry ; Epitopes, T-Lymphocyte/immunology ; Genotype ; HIV Infections/genetics ; HIV Infections/immunology ; HIV Infections/virology ; HIV-1/genetics ; HIV-1/immunology ; HLA Antigens/genetics ; HLA Antigens/immunology ; Host-Pathogen Interactions/genetics ; Host-Pathogen Interactions/immunology ; Humans ; Immunophenotyping ; Middle Aged ; Peptides/chemistry ; Peptides/immunology ; T-Lymphocytes, Cytotoxic/immunology ; T-Lymphocytes, Cytotoxic/metabolism
Chemical Substances	Epitopes, T-Lymphocyte ; HLA Antigens ; Peptides
Language	English
Publishing date	2021-01-12
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2851331-9
ISSN	2352-3964
ISSN (online)	2352-3964
DOI	10.1016/j.ebiom.2020.103175
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Article: Nimotuzumab Induces NK Cell Activation, Cytotoxicity, Dendritic Cell Maturation and Expansion of EGFR-Specific T Cells in Head and Neck Cancer Patients.

Mazorra, Zaima / Lavastida, Anabel / Concha-Benavente, Fernando / Valdés, Anet / Srivastava, Raghvendra M / García-Bates, Tatiana M / Hechavarría, Esperanza / González, Zuyen / González, Amnely / Lugiollo, Martha / Cuevas, Iván / Frómeta, Carlos / Mestre, Braulio F / Barroso, Maria C / Crombet, Tania / Ferris, Robert L

Frontiers in pharmacology

2017 Volume 8, Page(s) 382

Abstract: Survival benefit and long-term duration of clinical response have been seen using the epidermal growth factor receptor (EGFR)-targeted monoclonal antibody (mAb) nimotuzumab. Blocking EGFR signaling may not be the only mechanism of action underlying its ... ...

Abstract	Survival benefit and long-term duration of clinical response have been seen using the epidermal growth factor receptor (EGFR)-targeted monoclonal antibody (mAb) nimotuzumab. Blocking EGFR signaling may not be the only mechanism of action underlying its efficacy. As an IgG1 isotype mAb, nimotuzumab's capacity of killing tumor cells by antibody dependent cellular cytotoxicity (ADCC) and to induce an immune response in cancer patients have not been studied. ADCC-induced by nimotuzumab was determined using a
Language	English
Publishing date	2017-06-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2017.00382
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Article: Anti-EGFR Targeted Monoclonal Antibody Isotype Influences Antitumor Cellular Immunity in Head and Neck Cancer Patients.

Trivedi, Sumita / Srivastava, Raghvendra M / Concha-Benavente, Fernando / Ferrone, Soldano / Garcia-Bates, Tatiana M / Li, Jing / Ferris, Robert L

Clinical cancer research : an official journal of the American Association for Cancer Research

2016 Volume 22, Issue 21, Page(s) 5229–5237

Abstract: Purpose: EGF receptor (EGFR) is highly overexpressed on several cancers and two targeted anti-EGFR antibodies which differ by isotype are FDA-approved for clinical use. Cetuximab (IgG1 isotype) inhibits downstream signaling of EGFR and activates ... ...

Abstract	Purpose: EGF receptor (EGFR) is highly overexpressed on several cancers and two targeted anti-EGFR antibodies which differ by isotype are FDA-approved for clinical use. Cetuximab (IgG1 isotype) inhibits downstream signaling of EGFR and activates antitumor, cellular immune mechanisms. As panitumumab (IgG2 isotype) may inhibit downstream EGFR signaling similar to cetuximab, it might also induce adaptive immunity. Experimental design: We measured in vitro activation of cellular components of the innate and adaptive immune systems. We also studied the in vivo activation of components of the adaptive immune system in patient specimens from two recent clinical trials using cetuximab or panitumumab. Results: Both monoclonal antibodies (mAb) primarily activate natural killer (NK) cells, although cetuximab is significantly more potent than panitumumab. Cetuximab-activated neutrophils mediate antibody-dependent cellular cytotoxicity (ADCC) against head and neck squamous cell carcinomas (HNSCC) tumor cells, and interestingly, this effect was FcγRIIa- and FcγRIIIa genotype-dependent. Panitumumab may activate monocytes through CD32 (FcγRIIa); however, monocytes activated by either mAb are not able to mediate ADCC. Cetuximab enhanced dendritic cell (DC) maturation to a greater extent than panitumumab, which was associated with improved tumor antigen cross-presentation by cetuximab compared with panitumumab. This correlated with increased EGFR-specific cytotoxic CD8 Conclusions: Although panitumumab effectively inhibits EGFR signaling to a similar extent as cetuximab, it is less effective at triggering antitumor, cellular immune mechanisms which may be crucial for effective therapy of HNSCC. Clin Cancer Res; 22(21); 5229-37. ©2016 AACR.
MeSH term(s)	Antibodies, Monoclonal/immunology ; Antibodies, Monoclonal/therapeutic use ; CD8-Positive T-Lymphocytes/drug effects ; CD8-Positive T-Lymphocytes/immunology ; Carcinoma, Squamous Cell/drug therapy ; Carcinoma, Squamous Cell/immunology ; Cetuximab/immunology ; Cetuximab/therapeutic use ; Dendritic Cells/drug effects ; Dendritic Cells/immunology ; ErbB Receptors/antagonists & inhibitors ; ErbB Receptors/immunology ; Head and Neck Neoplasms/drug therapy ; Head and Neck Neoplasms/immunology ; Humans ; Immunity, Cellular/drug effects ; Immunity, Cellular/immunology ; Immunoglobulin G/immunology ; Killer Cells, Natural/drug effects ; Killer Cells, Natural/immunology ; Monocytes/drug effects ; Monocytes/immunology ; Panitumumab ; Receptors, IgG/immunology ; Squamous Cell Carcinoma of Head and Neck ; T-Lymphocytes, Cytotoxic/drug effects ; T-Lymphocytes, Cytotoxic/immunology
Chemical Substances	Antibodies, Monoclonal ; Fc gamma receptor IIA ; Fc gamma receptor IIC ; Immunoglobulin G ; Receptors, IgG ; Panitumumab (6A901E312A) ; EGFR protein, human (EC 2.7.10.1) ; ErbB Receptors (EC 2.7.10.1) ; Cetuximab (PQX0D8J21J)
Language	English
Publishing date	2016-05-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	1225457-5
ISSN	1557-3265 ; 1078-0432
ISSN (online)	1557-3265
ISSN	1078-0432
DOI	10.1158/1078-0432.CCR-15-2971
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Article ; Online: Enhanced Cytotoxic CD8 T Cell Priming Using Dendritic Cell-Expressing Human Papillomavirus-16 E6/E7-p16INK4 Fusion Protein with Sequenced Anti-Programmed Death-1.

Garcia-Bates, Tatiana M / Kim, Eun / Concha-Benavente, Fernando / Trivedi, Sumita / Mailliard, Robbie B / Gambotto, Andrea / Ferris, Robert L

Journal of immunology (Baltimore, Md. : 1950)

2016 Volume 196, Issue 6, Page(s) 2870–2878

Abstract: The incidence of human papillomavirus (HPV)-related head and neck squamous cell carcinoma has increased in recent decades, though HPV prevention vaccines may reduce this rise in the future. HPV-related cancers express the viral oncoproteins E6 and E7. ... ...

Abstract	The incidence of human papillomavirus (HPV)-related head and neck squamous cell carcinoma has increased in recent decades, though HPV prevention vaccines may reduce this rise in the future. HPV-related cancers express the viral oncoproteins E6 and E7. The latter inactivates the tumor suppressor protein retinoblastoma (Rb), which leads to the overexpression of p16(INK4) protein, providing unique Ags for therapeutic HPV-specific cancer vaccination. We developed potential adenoviral vaccines that express a fusion protein of HPV-16 E6 and E7 (Ad.E6E7) alone or fused with p16 (Ad.E6E7p16) and also encoding an anti-programmed death (PD)-1 Ab. Human monocyte-derived dendritic cells (DC) transduced with Ad.E6E7 or Ad.E6E7p16 with or without Ad.αPD1 were used to activate autologous CD8 CTL in vitro. CTL responses were tested against naturally HPV-infected head and neck squamous cell carcinoma cells using IFN-γ ELISPOT and [(51)Cr]release assay. Surprisingly, stimulation and antitumor activity of CTL were increased after incubation with Ad.E6E7p16-transduced DC (DC.E6E7p16) compared with Ad.E6E7 (DC.E6E7), a result that may be due to an effect of p16 on cyclin-dependent kinase 4 levels and IL-12 secretion by DC. Moreover, the beneficial effect was most prominent when anti-PD-1 was introduced during the second round of stimulation (after initial priming). These data suggest that careful sequencing of Ad.E6E7.p16 with Ad.αPD1 could improve antitumor immunity against HPV-related tumors and that p16 may enhance the immunogenicity of DC, through cyclin-dependent pathways, Th1 cytokine secretion, and by adding a nonviral Ag highly overexpressed in HPV-induced cancers.
MeSH term(s)	Adenoviridae/genetics ; Antibodies/genetics ; Antibodies/metabolism ; CD8-Positive T-Lymphocytes/immunology ; Cancer Vaccines/administration & dosage ; Cyclin-Dependent Kinase 4/metabolism ; Cyclin-Dependent Kinase Inhibitor p16/genetics ; Cyclin-Dependent Kinase Inhibitor p16/metabolism ; Cytotoxicity, Immunologic ; Dendritic Cells/immunology ; Dendritic Cells/transplantation ; Head and Neck Neoplasms/immunology ; Head and Neck Neoplasms/therapy ; Human papillomavirus 16/immunology ; Humans ; Interferon-gamma/metabolism ; Interleukin-12/metabolism ; Mutation/genetics ; Neoplasms, Squamous Cell/immunology ; Neoplasms, Squamous Cell/therapy ; Oncogene Proteins, Viral/genetics ; Oncogene Proteins, Viral/metabolism ; Papillomavirus Infections/immunology ; Papillomavirus Infections/therapy ; Programmed Cell Death 1 Receptor/immunology ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; Repressor Proteins/genetics ; Repressor Proteins/metabolism
Chemical Substances	Antibodies ; Cancer Vaccines ; Cyclin-Dependent Kinase Inhibitor p16 ; E6 protein, Human papillomavirus type 16 ; E7 protein, Human papillomavirus type 33 ; Oncogene Proteins, Viral ; PDCD1 protein, human ; Programmed Cell Death 1 Receptor ; Recombinant Fusion Proteins ; Repressor Proteins ; Interleukin-12 (187348-17-0) ; Interferon-gamma (82115-62-6) ; Cyclin-Dependent Kinase 4 (EC 2.7.11.22)
Language	English
Publishing date	2016-03-15
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	3056-9
ISSN	1550-6606 ; 0022-1767 ; 1048-3233 ; 1047-7381
ISSN (online)	1550-6606
ISSN	0022-1767 ; 1048-3233 ; 1047-7381
DOI	10.4049/jimmunol.1502027
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