LIVIVO - Search results -

Search results

Result 1 - 10 of total 18

Search options

Article ; Online: A large travel-associated outbreak of iatrogenic botulism in four European countries following intragastric botulinum neurotoxin injections for weight reduction, Türkiye, February to March 2023.

Dorner, Martin Bernhard / Wilking, Hendrik / Skiba, Martin / Wilk, Laura / Steinberg, Maximilian / Worbs, Sylvia / Çeken, Sabahat / Kaygusuz, Sedat / Simon, Stéphanie / Becher, François / Mikolajewska, Agata / Kornschober, Christian / Bütler, Timo / Jourdan-Da-Silva, Nathalie / An der Heiden, Maria / Schaade, Lars / Stark, Klaus / Dorner, Brigitte Gertrud / Frank, Christina

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin

2023 Volume 28, Issue 23

Abstract: In March 2023, 34 associated cases of iatrogenic botulism were detected in Germany (30 cases), Switzerland (two cases), Austria (one case), and France (one case). An alert was rapidly disseminated via European Union networks and communication platforms ( ... ...

Abstract	In March 2023, 34 associated cases of iatrogenic botulism were detected in Germany (30 cases), Switzerland (two cases), Austria (one case), and France (one case). An alert was rapidly disseminated via European Union networks and communication platforms (Food- and Waterborne Diseases and Zoonoses Network, EpiPulse, Early Warning and Response System) and the International Health Regulation mechanism; the outbreak was investigated in a European collaboration. We traced sources of the botulism outbreak to treatment of weight loss in Türkiye, involving intragastric injections of botulinum neurotoxin. Cases were traced using a list of patients who had received this treatment. Laboratory investigations of the first 12 German cases confirmed nine cases. The application of innovative and highly sensitive endopeptidase assays was necessary to detect minute traces of botulinum neurotoxin in patient sera. The botulism notification requirement for physicians was essential to detect this outbreak in Germany. The surveillance case definition of botulism should be revisited and inclusion of cases of iatrogenic botulism should be considered as these cases might lack standard laboratory confirmation yet warrant public health action. Any potential risks associated with the use of botulinum neurotoxins in medical procedures need to be carefully balanced with the expected benefits of the procedure.
MeSH term(s)	Animals ; Humans ; Botulinum Toxins/adverse effects ; Botulism/diagnosis ; Botulism/epidemiology ; Botulism/etiology ; Neurotoxins ; Travel ; Disease Outbreaks ; Weight Loss ; Iatrogenic Disease/epidemiology ; Clostridium botulinum
Chemical Substances	Botulinum Toxins (EC 3.4.24.69) ; Neurotoxins
Language	English
Publishing date	2023-06-08
Publishing country	Sweden
Document type	Journal Article
ZDB-ID	1338803-4
ISSN	1560-7917 ; 1025-496X
ISSN (online)	1560-7917
ISSN	1025-496X
DOI	10.2807/1560-7917.ES.2023.28.23.2300203
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5066: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article: Protection of dystrophic muscle cells with polyphenols from green tea correlates with improved glutathione balance and increased expression of 67LR, a receptor for (-)-epigallocatechin gallate.

Dorchies, Olivier M / Wagner, Stéphanie / Buetler, Timo M / Ruegg, Urs T

BioFactors (Oxford, England)

2009 Volume 35, Issue 3, Page(s) 279–294

Abstract: Duchenne muscular dystrophy (DMD) is a fatal muscle wasting disease caused by the absence of the protein dystrophin. Because oxidative stress contributes to the pathogenesis of DMD, we investigated if a green tea polyphenol blend (GTP) and its major ... ...

Abstract	Duchenne muscular dystrophy (DMD) is a fatal muscle wasting disease caused by the absence of the protein dystrophin. Because oxidative stress contributes to the pathogenesis of DMD, we investigated if a green tea polyphenol blend (GTP) and its major polyphenol (-)-epigallocatechin gallate (EGCg), could protect muscle cell primary cultures from oxidative damage induced by hydrogen peroxide (H(2)O(2)) in the widely used mdx mouse model. On-line fluorimetric measurements using an H(2)O(2)-sensitive probe indicated that GTP and EGCg scavenged peroxide in a concentration-dependent manner. A 48 h exposure to EGCg increased glutathione content but did not alter the expression of proteins involved in membrane stabilization and repair. Pretreatment of dystrophic cultures with GTP or EGCg 48 h before exposure to H(2)O(2) improved cell survival. Normal cultures were protected by GTP but not by EGCg. 67LR, a receptor for EGCg, was seven times more abundant in dystrophic compared with normal cultures. Altogether our results demonstrate that GTP and EGCg protect muscle cells by scavenging H(2)O(2) and by improving the glutathione balance. In addition, the higher levels of 67LR in dystrophic muscle cells compared with normal ones likely contribute to EGCg-mediated survival.
MeSH term(s)	Animals ; Animals, Newborn ; Catechin/analogs & derivatives ; Catechin/pharmacology ; Cell Survival/drug effects ; Cells, Cultured ; Flavonoids/pharmacology ; Glutathione/metabolism ; Hydrogen Peroxide/pharmacology ; Immunohistochemistry ; Mice ; Mice, Inbred C57BL ; Mice, Inbred mdx ; Mice, Mutant Strains ; Muscle Cells/drug effects ; Muscle Cells/metabolism ; Muscle, Skeletal/cytology ; Oxidative Stress/drug effects ; Phenols/pharmacology ; Polyphenols ; Protease Inhibitors/pharmacology ; Receptors, Laminin/metabolism ; Tea/chemistry
Chemical Substances	Flavonoids ; Phenols ; Polyphenols ; Protease Inhibitors ; Receptors, Laminin ; Tea ; Catechin (8R1V1STN48) ; Hydrogen Peroxide (BBX060AN9V) ; epigallocatechin gallate (BQM438CTEL) ; Glutathione (GAN16C9B8O)
Language	English
Publishing date	2009-05
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	59230-4
ISSN	1872-8081 ; 0951-6433
ISSN (online)	1872-8081
ISSN	0951-6433
DOI	10.1002/biof.34
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2473: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Role of superoxide as a signaling molecule.

Buetler, Timo M / Krauskopf, Alexandra / Ruegg, Urs T

News in physiological sciences : an international journal of physiology produced jointly by the International Union of Physiological Sciences and the American Physiological Society

2004 Volume 19, Page(s) 120–123

Abstract: Superoxide is known to affect vascular physiology in several ways and has also been recognized to contribute significantly to vascular physiopathology. Here we discuss the emerging role of superoxide as an essential signaling molecule in normal ... ...

Abstract	Superoxide is known to affect vascular physiology in several ways and has also been recognized to contribute significantly to vascular physiopathology. Here we discuss the emerging role of superoxide as an essential signaling molecule in normal physiology.
MeSH term(s)	Animals ; Cell Division/physiology ; Humans ; Signal Transduction/physiology ; Superoxides/metabolism
Chemical Substances	Superoxides (11062-77-4)
Language	English
Publishing date	2004-05-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	632842-8
ISSN	1522-161X ; 0886-1714
ISSN (online)	1522-161X
ISSN	0886-1714
DOI	10.1152/nips.01514.2003
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2164: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Dicarbonyls stimulate cellular protection systems in primary rat hepatocytes and show anti-inflammatory properties.

Buetler, Timo M / Latado, Hélia / Baumeyer, Alexandra / Delatour, Thierry

Annals of the New York Academy of Sciences

2008 Volume 1126, Page(s) 113–117

Abstract: Advanced glycation endproducts (AGEs) and their precursor dicarbonyls are generally perceived as having adverse health effects. They are also considered to be initiators and promoters of disease and aging. However, proof for a causal relationship is ... ...

Abstract	Advanced glycation endproducts (AGEs) and their precursor dicarbonyls are generally perceived as having adverse health effects. They are also considered to be initiators and promoters of disease and aging. However, proof for a causal relationship is lacking. On the other hand, it is known that AGEs and melanoidins possess beneficial properties, such as antioxidant and metal-chelating activities. Furthermore, some AGEs may stimulate the cellular detoxification system, generally known as the phase II drug metabolizing system. We show here that several reactive dicarbonyl intermediates have the capability to stimulate the cellular phase II detoxification systems in both a reporter cell line and primary rat hepatocytes. In addition, we demonstrate that dicarbonyls can attenuate the inflammatory signaling induced by tumor necrosis factor-alpha in a reporter cell system.
MeSH term(s)	Animals ; Anti-Inflammatory Agents/pharmacology ; Cell Line ; Cells, Cultured ; Gene Expression Regulation ; Genes, Reporter ; Glycation End Products, Advanced/pharmacology ; Hepatocytes/cytology ; Hepatocytes/drug effects ; Hepatocytes/physiology ; Hydroquinones/pharmacology ; Polymerase Chain Reaction ; RNA/genetics ; RNA, Messenger/genetics ; Rats ; TATA-Box Binding Protein/genetics
Chemical Substances	Anti-Inflammatory Agents ; Glycation End Products, Advanced ; Hydroquinones ; RNA, Messenger ; TATA-Box Binding Protein ; RNA (63231-63-0) ; 2-tert-butylhydroquinone (C12674942B)
Language	English
Publishing date	2008-04
Publishing country	United States
Document type	Journal Article
ZDB-ID	211003-9
ISSN	1749-6632 ; 0077-8923
ISSN (online)	1749-6632
ISSN	0077-8923
DOI	10.1196/annals.1433.036
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Se 110: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Glycolaldehyde-modified β-lactoglobulin AGEs are unable to stimulate inflammatory signaling pathways in RAGE-expressing human cell lines

Buetler, Timo M / Latado, Hélia / Leclerc, Estelle / Weigle, Bernd / Baumeyer, Alexandra / Heizmann, Claus W / Scholz, Gabriele

Molecular nutrition & food research. 2011 Feb., v. 55, no. 2

2011

Abstract: Scope: Advanced glycation endproducts (AGEs) are suspected to stimulate inflammatory signaling pathways in target tissues via activation of the receptor for AGEs. Endotoxins are generally recognized as potential contamination of AGE preparations and ... ...

Abstract	Scope: Advanced glycation endproducts (AGEs) are suspected to stimulate inflammatory signaling pathways in target tissues via activation of the receptor for AGEs. Endotoxins are generally recognized as potential contamination of AGE preparations and stimulate biological actions that are very similar as or identical to those induced by AGEs. Methods and results: In our study, we used glycolaldehyde-modified β-lactoglobulin preparations as model AGEs and employed two methods to remove endotoxin using either affinity columns or extraction with Triton X-114 (TX-114). Affinity column-purified AGEs retained their ability to stimulate inflammatory signaling as measured by mRNA expression of inflammatory cytokines in the human lung epithelial cell line Beas2b. However, glycolaldehyde-modified AGEs purified by extraction with TX-114 did not show any stimulation of mRNA expression of inflammatory cytokines. The presence of a cell stimulating endotoxin-like activity was demonstrated in the detergent phase after extraction with TX-114, thus indicating that not AGEs but a lipophilic contamination was responsible for the stimulation of inflammatory signaling. Conclusion: Our results demonstrate that glycolaldehyde-modified AGEs are unable to induce inflammatory signaling in receptor for AGE-expressing cells. The observed cell-activating activity can be ascribed to an endotoxin-like lipophilic contamination present in AGE preparations and affinity column purification was insufficient to remove this contamination.
Keywords	cytokines ; endotoxins ; epithelial cells ; gene expression ; glycation ; human cell lines ; humans ; lungs ; messenger RNA ; receptors
Language	English
Dates of publication	2011-02
Size	p. 291-299.
Publishing place	Wiley-VCH Verlag
Document type	Article
ZDB-ID	2160372-8
ISSN	1613-4133 ; 1613-4125
ISSN (online)	1613-4133
ISSN	1613-4125
DOI	10.1002/mnfr.201000140
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Glycolaldehyde-modified β-lactoglobulin AGEs are unable to stimulate inflammatory signaling pathways in RAGE-expressing human cell lines.

Buetler, Timo M / Latado, Hélia / Leclerc, Estelle / Weigle, Bernd / Baumeyer, Alexandra / Heizmann, Claus W / Scholz, Gabriele

Molecular nutrition & food research

2011 Volume 55, Issue 2, Page(s) 291–299

Abstract	Scope: Advanced glycation endproducts (AGEs) are suspected to stimulate inflammatory signaling pathways in target tissues via activation of the receptor for AGEs. Endotoxins are generally recognized as potential contamination of AGE preparations and stimulate biological actions that are very similar as or identical to those induced by AGEs. Methods and results: In our study, we used glycolaldehyde-modified β-lactoglobulin preparations as model AGEs and employed two methods to remove endotoxin using either affinity columns or extraction with Triton X-114 (TX-114). Affinity column-purified AGEs retained their ability to stimulate inflammatory signaling as measured by mRNA expression of inflammatory cytokines in the human lung epithelial cell line Beas2b. However, glycolaldehyde-modified AGEs purified by extraction with TX-114 did not show any stimulation of mRNA expression of inflammatory cytokines. The presence of a cell stimulating endotoxin-like activity was demonstrated in the detergent phase after extraction with TX-114, thus indicating that not AGEs but a lipophilic contamination was responsible for the stimulation of inflammatory signaling. Conclusion: Our results demonstrate that glycolaldehyde-modified AGEs are unable to induce inflammatory signaling in receptor for AGE-expressing cells. The observed cell-activating activity can be ascribed to an endotoxin-like lipophilic contamination present in AGE preparations and affinity column purification was insufficient to remove this contamination.
MeSH term(s)	Acetaldehyde/analogs & derivatives ; Acetaldehyde/chemistry ; Cell Line ; Cytokines/genetics ; Cytokines/metabolism ; Detergents/chemistry ; Endotoxins/isolation & purification ; Endotoxins/pharmacology ; Glycation End Products, Advanced/chemistry ; Glycation End Products, Advanced/isolation & purification ; Glycation End Products, Advanced/metabolism ; Humans ; Inflammation/metabolism ; Lactoglobulins/chemistry ; Lactoglobulins/isolation & purification ; Lactoglobulins/metabolism ; Polyethylene Glycols/chemistry ; RNA, Messenger/metabolism ; Receptor for Advanced Glycation End Products ; Receptors, Immunologic/metabolism ; Reproducibility of Results ; Signal Transduction
Chemical Substances	Cytokines ; Detergents ; Endotoxins ; Glycation End Products, Advanced ; Lactoglobulins ; RNA, Messenger ; Receptor for Advanced Glycation End Products ; Receptors, Immunologic ; Polyethylene Glycols (30IQX730WE) ; Nonidet P-40 (9036-19-5) ; Acetaldehyde (GO1N1ZPR3B) ; glycolaldehyde (W0A0XPU08U)
Language	English
Publishing date	2011-02
Publishing country	Germany
Document type	Comparative Study ; Journal Article
ZDB-ID	2160372-8
ISSN	1613-4133 ; 1613-4125
ISSN (online)	1613-4133
ISSN	1613-4125
DOI	10.1002/mnfr.201000140
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article: Supplementation with oral probiotic bacteria maintains cutaneous immune homeostasis after UV exposure.

Guéniche, Audrey / Benyacoub, Jalil / Buetler, Timo M / Smola, Hans / Blum, Stephanie

European journal of dermatology : EJD

2006 Volume 16, Issue 5, Page(s) 511–517

Abstract: Probiotic bacteria have been shown to modulate the immune system of the gut and protect against infectious and inflammatory diseases of the gastro-intestinal tract. Ultraviolet radiation (UVR) is known to alter the cutaneous and systemic immune systems ... ...

Abstract	Probiotic bacteria have been shown to modulate the immune system of the gut and protect against infectious and inflammatory diseases of the gastro-intestinal tract. Ultraviolet radiation (UVR) is known to alter the cutaneous and systemic immune systems implicated in the development of skin tumors. In this study we investigated whether oral probiotics are able to modulate the immune system of the skin using hairless Skh:hr1 mice exposed to an acute dose of UVR. We show that nutritional supplementation with Lactobacillus johnsonii (La1) at 10(8) cfu/day for 10 days was able to protect against the UVR-induced suppression of contact hypersensitivity, the decreased epidermal Langerhans cell density and the increased IL-10 serum levels. In the absence of UV exposure, probiotic bacteria had no detectable effect on the immune system of the skin, thus acting only to re-establish skin homeostasis. In conclusion, our data demonstrate that ingested probiotic bacteria can maintain cutaneous immune capacity after UV exposure.
MeSH term(s)	Animals ; Dermatitis, Contact/immunology ; Female ; Homeostasis/drug effects ; Homeostasis/radiation effects ; Immunity, Innate/drug effects ; Immunity, Innate/radiation effects ; Inflammation/physiopathology ; Interleukin-10/blood ; Lactobacillus ; Langerhans Cells/physiology ; Mice ; Mice, Hairless ; Mice, Inbred Strains ; Probiotics/therapeutic use ; Skin Physiological Phenomena/drug effects ; Skin Physiological Phenomena/radiation effects ; Ultraviolet Rays
Chemical Substances	Interleukin-10 (130068-27-8)
Language	English
Publishing date	2006-09
Publishing country	France
Document type	Journal Article
ZDB-ID	1128666-0
ISSN	1952-4013 ; 1167-1122
ISSN (online)	1952-4013
ISSN	1167-1122
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3484: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Cyclosporin A generates superoxide in smooth muscle cells.

Krauskopf, Alexandra / Lhote, Philippe / Petermann, Olivier / Ruegg, Urs T / Buetler, Timo M

Free radical research

2005 Volume 39, Issue 9, Page(s) 913–919

Abstract: Cyclosporin A (CsA) generates superoxide in smooth muscle cells. Our earlier studies have demonstrated that the increase in the vasopressin type 1 receptor induced in vascular smooth muscle cells in the presence of CsA is probably due to superoxide ( ... ...

Abstract	Cyclosporin A (CsA) generates superoxide in smooth muscle cells. Our earlier studies have demonstrated that the increase in the vasopressin type 1 receptor induced in vascular smooth muscle cells in the presence of CsA is probably due to superoxide (Krauskopf et al., J Biol Chem 278, 41685-41690, 2003). This increase in vasopressin receptor is likely at the base of increased vascular responsiveness to vasoconstrictor hormones and hypertension induced by CsA. Here, we demonstrate that CsA produces superoxide. In addition, our data show that superoxide generation does not originate from the major cellular superoxide generating systems NAD(P)H oxidase or xanthine oxidase. Our results suggest that the side effects of CsA could be diminished with the help of SOD mimetic drugs.
MeSH term(s)	Animals ; Aorta/drug effects ; Cells, Cultured ; Cyclosporine/pharmacology ; Dose-Response Relationship, Drug ; Ethidium/analogs & derivatives ; Fluoresceins ; Free Radical Scavengers/pharmacology ; Immunosuppressive Agents/pharmacology ; Male ; Myocytes, Smooth Muscle/drug effects ; Myocytes, Smooth Muscle/metabolism ; Rats ; Reactive Oxygen Species/metabolism ; Superoxide Dismutase/antagonists & inhibitors ; Superoxide Dismutase/genetics ; Superoxides/metabolism
Chemical Substances	Fluoresceins ; Free Radical Scavengers ; Immunosuppressive Agents ; Reactive Oxygen Species ; dihydroethidium (104821-25-2) ; Superoxides (11062-77-4) ; diacetyldichlorofluorescein (2044-85-1) ; Cyclosporine (83HN0GTJ6D) ; Superoxide Dismutase (EC 1.15.1.1) ; Ethidium (EN464416SI)
Language	English
Publishing date	2005-09
Publishing country	England
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1194130-3
ISSN	1029-2470 ; 1071-5762
ISSN (online)	1029-2470
ISSN	1071-5762
DOI	10.1080/10715760500104009
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2236: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: International STakeholder NETwork (ISTNET): creating a developmental neurotoxicity (DNT) testing road map for regulatory purposes.

Bal-Price, Anna / Crofton, Kevin M / Leist, Marcel / Allen, Sandra / Arand, Michael / Buetler, Timo / Delrue, Nathalie / FitzGerald, Rex E / Hartung, Thomas / Heinonen, Tuula / Hogberg, Helena / Bennekou, Susanne Hougaard / Lichtensteiger, Walter / Oggier, Daniela / Paparella, Martin / Axelstad, Marta / Piersma, Aldert / Rached, Eva / Schilter, Benoît /

Schmuck, Gabriele / Stoppini, Luc / Tongiorgi, Enrico / Tiramani, Manuela / Monnet-Tschudi, Florianne / Wilks, Martin F / Ylikomi, Timo / Fritsche, Ellen

Archives of toxicology

2015 Volume 89, Issue 2, Page(s) 269–287

Abstract: A major problem in developmental neurotoxicity (DNT) risk assessment is the lack of toxicological hazard information for most compounds. Therefore, new approaches are being considered to provide adequate experimental data that allow regulatory decisions. ...

Abstract	A major problem in developmental neurotoxicity (DNT) risk assessment is the lack of toxicological hazard information for most compounds. Therefore, new approaches are being considered to provide adequate experimental data that allow regulatory decisions. This process requires a matching of regulatory needs on the one hand and the opportunities provided by new test systems and methods on the other hand. Alignment of academically and industrially driven assay development with regulatory needs in the field of DNT is a core mission of the International STakeholder NETwork (ISTNET) in DNT testing. The first meeting of ISTNET was held in Zurich on 23-24 January 2014 in order to explore the concept of adverse outcome pathway (AOP) to practical DNT testing. AOPs were considered promising tools to promote test systems development according to regulatory needs. Moreover, the AOP concept was identified as an important guiding principle to assemble predictive integrated testing strategies (ITSs) for DNT. The recommendations on a road map towards AOP-based DNT testing is considered a stepwise approach, operating initially with incomplete AOPs for compound grouping, and focussing on key events of neurodevelopment. Next steps to be considered in follow-up activities are the use of case studies to further apply the AOP concept in regulatory DNT testing, making use of AOP intersections (common key events) for economic development of screening assays, and addressing the transition from qualitative descriptions to quantitative network modelling.
MeSH term(s)	Brain/drug effects ; Fetus/drug effects ; Guidelines as Topic ; Humans ; Neurotoxicity Syndromes/etiology ; Risk Assessment ; Toxicity Tests/methods
Language	English
Publishing date	2015-02
Publishing country	Germany
Document type	Journal Article
ZDB-ID	124992-7
ISSN	1432-0738 ; 0340-5761
ISSN (online)	1432-0738
ISSN	0340-5761
DOI	10.1007/s00204-015-1464-2
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Uf I Zs.90: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Vasopressin type 1A receptor up-regulation by cyclosporin A in vascular smooth muscle cells is mediated by superoxide.

Krauskopf, Alexandra / Lhote, Philippe / Mutter, Manfred / Dufour, Jean-François / Ruegg, Urs T / Buetler, Timo M

The Journal of biological chemistry

2003 Volume 278, Issue 43, Page(s) 41685–41690

Abstract: Based on our previous results, we investigated whether cyclosporin A (CsA)-induced vasopressin type 1A receptor up-regulation was mediated by free radicals. We report that CsA analogues with different affinities for cyclophilin and calcineurin were able ... ...

Abstract	Based on our previous results, we investigated whether cyclosporin A (CsA)-induced vasopressin type 1A receptor up-regulation was mediated by free radicals. We report that CsA analogues with different affinities for cyclophilin and calcineurin were able to up-regulate vasopressin type 1A receptor and to generate free radicals in smooth muscle cells independently of calcineurin. Further, we demonstrate that the antioxidant N-acetyl-L-cysteine blocked the increase in vasopressin type 1A receptor mRNA and protein levels induced by CsA and that low concentrations of prooxidants were able to directly increase vasopressin type 1A receptor mRNA and protein levels. In addition, short exposure to CsA or pro-oxidants was sufficient to significantly increase vasopressin type 1A receptor mRNA and protein levels. Using cell-permeable forms of superoxide dismutase and catalase, we finally show that superoxide mediates the CsA-induced effects on vasopressin type 1A receptor. These results provide strong evidence that CsA-induced superoxide generation is causally involved in vasopressin type 1A receptor expression and demonstrate for the first time that low physiological concentrations of radicals, most probably superoxide, are able to directly affect cellular signaling to increase vasopressin type 1A receptor expression in rat aortic smooth muscle cells.
MeSH term(s)	Animals ; Aorta/cytology ; Calcineurin/pharmacology ; Catalase/metabolism ; Cyclophilins/pharmacology ; Cyclosporine/pharmacology ; Free Radicals/metabolism ; Male ; Muscle, Smooth, Vascular/cytology ; RNA, Messenger/biosynthesis ; RNA, Messenger/drug effects ; Rats ; Rats, Wistar ; Receptors, Vasopressin/biosynthesis ; Superoxide Dismutase/metabolism ; Superoxides/metabolism ; Superoxides/pharmacology ; Up-Regulation/drug effects
Chemical Substances	Free Radicals ; RNA, Messenger ; Receptors, Vasopressin ; Superoxides (11062-77-4) ; Cyclosporine (83HN0GTJ6D) ; Catalase (EC 1.11.1.6) ; Superoxide Dismutase (EC 1.15.1.1) ; Calcineurin (EC 3.1.3.16) ; Cyclophilins (EC 5.2.1.-)
Language	English
Publishing date	2003-08-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.M301181200
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uc I Zs.108: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito