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  1. Article ; Online: Indirect effect of diabatic heating on Mei-yu frontogenesis

    Hu, Yang / Deng, Yi / Lin, Yanluan / Zhou, Zhimin / Cui, Chunguang / Li, Chao / Dong, Xiquan

    Clim Dyn. 2022 Aug., v. 59, no. 3-4 p.851-868

    2022  

    Abstract: Mei-yu fronts are accompanied with the formation of frontal clouds. In addition to its direct ... effect on Mei-yu fronts through the modification of atmospheric temperature, diabatic heating related ... contributions to the Mei-yu frontogenesis. The indirect effect of diabatic heating on Mei-yu frontogenesis is ...

    Abstract Mei-yu fronts are accompanied with the formation of frontal clouds. In addition to its direct effect on Mei-yu fronts through the modification of atmospheric temperature, diabatic heating related to clouds formation also influences frontogenesis indirectly by changing local atmospheric circulation. To quantify such indirect effects, piecewise potential vorticity (PV) inversion is adopted to decompose the process of deformation frontogenesis into several parts associated with distinct PV anomalies. The balanced flow associated with the interior-level diabatic PV anomaly emerges as the most stable and important contributor to the total deformation frontogenesis with the effect of local diabatic PV anomaly in the frontal zone outweighing the effect of remote diabatic PV anomaly. Lower-boundary thermal anomaly (i.e., surface cooling associated with frontal clouds formation) and mean flow provide weak negative and positive contributions to the deformation frontogenesis, respectively. The balanced flow associated with the upper-level PV perturbations is weak at lower-levels, especially in the vicinity of the front zone and thus has negligible contributions to the Mei-yu frontogenesis. The indirect effect of diabatic heating on Mei-yu frontogenesis is generally weaker in magnitude compared to the direct effect of temperature modification as well as the impact of moisture depletion that is also tied to clouds formation. The results presented here add further evidences about the importance of cloud feedback to the evolution of Mei-yu fronts and suggest the necessity of improved model representations of cloud processes in achieving a better simulation and prediction of Mei-yu rainfall.
    Keywords air temperature ; atmospheric circulation ; cooling ; deformation ; evolution ; heat ; prediction ; rain
    Language English
    Dates of publication 2022-08
    Size p. 851-868.
    Publishing place Springer Berlin Heidelberg
    Document type Article ; Online
    ZDB-ID 1471747-5
    ISSN 1432-0894 ; 0930-7575
    ISSN (online) 1432-0894
    ISSN 0930-7575
    DOI 10.1007/s00382-022-06159-7
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  2. Article ; Online: Research based on data mining compatibility of traditional Chinese medicine with Professor WANG Dong-mei in the treatment of primary dysmenorrhea

    Huan Gao / Dong-Mei Wang

    Journal of Hainan Medical University, Vol 27, Iss 20, Pp 50-

    2021  Volume 54

    Abstract: ... of primary dysmenorrhea by Professor WANG Dong‑mei. Methods:This study collected the Professor WANG Dong‑mei ... common clustering groups. Conclusion:Professor WANG Dong‑mei usually chooses the drugs of sweet,warm or ... combination is Shaofu Zhuyu Decoction,which embodies Professor WANG Dong‑mei's academic thought of treating ...

    Abstract Objective: To study the law of compatibility of traditional Chinese medicine in the treatment of primary dysmenorrhea by Professor WANG Dong‑mei. Methods:This study collected the Professor WANG Dong‑mei’s prescriptions for primary dysmenorrhea cases in the outpatient department,to establish a database by Excel 2015,and to use IBM SPSS statistics 24.0 and SPSS Clementine 12.0 software for clustering analysis and association rules data mining analysis. Results:A total of 106 cases were included,106 prescriptions were collected,114 kinds of drugs were used,nd the cumulative frequency of drug use was 1 876 times;the top three drugs were white peony(89 times),Xiangfu(77 times),red peony(75 times);the drugs with the most drug properties and taste frequency were warm and sweet drugs,which were mainly liver meridian;four common pair‑used drug groups were Xiangfu yanhusuo,angelica white peony etc. and six groups were common clustering groups. Conclusion:Professor WANG Dong‑mei usually chooses the drugs of sweet,warm or spicy in the treatment of primary dysmenorrhea. In addition to the natural efficacy,Prof. Wang also considers modern pharmacological effect in the specific drug selection. The commonly used prescription combination is Shaofu Zhuyu Decoction,which embodies Professor WANG Dong‑mei's academic thought of treating primary dysmenorrhea "mainly by warming and regulating qi and blood",and for patients with reproductive needs,in addition to promoting blood circulation and relieving pain,she also adds menstruation regulating and pregnancy aiding drugs,which can not only improve the symptoms of primary dysmenorrhea,but also meet their fertility desire.
    Keywords wang dong-mei ; primary dysmenorrhea ; data mining ; compatibility of traditional chinese medicine ; Medicine ; R
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Editorial Board of Journal of Hainan Medical University
    Document type Article ; Online
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  3. Article ; Online: Network Pharmacology Research Indicates that Wu-Mei-Wan Treats Obesity by Inhibiting Th17 Cell Differentiation and Alleviating Metabolic Inflammation.

    Cheng, Zhe / Xiong, Xinyu / Wu, Fan / Zhao, Yan / Dong, Ruolan / Jiang, Shujun / Fang, Ke / Huang, Panpan / Chen, Guang

    Combinatorial chemistry & high throughput screening

    2022  Volume 26, Issue 1, Page(s) 30–48

    Abstract: Background: Wu-Mei-Wan (WMW), a traditional Chinese medicine (TCM) formula, has a good effect ...

    Abstract Background: Wu-Mei-Wan (WMW), a traditional Chinese medicine (TCM) formula, has a good effect on the treatment of obesity and has been proven helpful to promote the metabolism of adipose tissue. However, its underlying mechanism remains to be studied. This study aims to explore the potential pharmacological mechanism of WMW in the treatment of obesity.
    Methods: Network pharmacology was used to sort out the relationship between WMW putative targets and obesity-related drug targets or disease targets, which indicated the mechanism of WMW in treating obesity from two aspects of clinical drugs approved by the Food and Drug Administration (FDA) and obesity-related diseases. Databases such as Traditional Chinese Medicine Systems Pharmacology (TCMSP), PubChem, DrugBank, DisGeNET, and Genecards were used to collect information about targets. String platform was used to convert the data into gene symbol of "homo sapiens", and perform gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. With the Human Protein Reference Database (HPRD) as background data, Cytoscape 3.6.0 software was used to construct a new protein-protein interaction (PPI) network. Mechanism diagrams of key pathways were obtained from the KEGG database. AutoDock Vina software was used to conduct molecular docking verification.
    Results: The number of targets in the overlap between WMW putative targets and obesity-related drug targets accounted for more than 50% of the latter, and HTR3A, SLC6A4, and CYP3A4 were core targets. In obesity-related disease targets-WMW putative targets PPI network, the Th17 cell differentiation pathway, and the IL-17 signaling pathway were key pathways, and the 1st module and the 7th module were central function modules that were highly associated with immunity and inflammation. Molecular docking verified that STAT3, TGFB1, MMP9, AHR, IL1B, and CCL2 were core targets in the treatment of WMW on obesity.
    Conclusion: WMW has similar effects on lipid and drug metabolism as the current obesity-related drugs, and is likely to treat obesity by inhibiting Th17 cell differentiation and alleviating metabolic inflammation.
    MeSH term(s) United States ; Humans ; Network Pharmacology ; Molecular Docking Simulation ; Signal Transduction ; Cell Differentiation ; Databases, Protein ; Serotonin Plasma Membrane Transport Proteins
    Chemical Substances SLC6A4 protein, human ; Serotonin Plasma Membrane Transport Proteins
    Language English
    Publishing date 2022-02-16
    Publishing country United Arab Emirates
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2064785-2
    ISSN 1875-5402 ; 1386-2073
    ISSN (online) 1875-5402
    ISSN 1386-2073
    DOI 10.2174/1386207325666220221121919
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  4. Book ; Online: Idealized simulations of Mei-yu rainfall in Taiwan under uniform southwesterly flow using a cloud-resolving model

    Wang, Chung-Chieh / Chuang, Pi-Yu / Chen, Shi-Ting / Lee, Dong-In / Tsuboki, Kazuhisa

    eISSN: 1684-9981

    2022  

    Abstract: ... and the role of the complex topography in Taiwan during the Mei-yu season without the influence ...

    Abstract In this study, idealized cloud-resolving simulations are performed for horizontally uniform and steady southwesterly flow at fixed direction–speed combinations to investigate rainfall characteristics and the role of the complex topography in Taiwan during the Mei-yu season without the influence of a front or other disturbances. Eight directions (180 to 285 ∘ , every 15 ∘ ) and eight speeds (5 to 22.5 m s −1 , every 2.5 m s −1 ) are considered, and near-surface relative humidity is also altered (from 55 %–100 %) in a subset of these tests to further examine the effects of moisture content, yielding a total 109 experiments each having a integration length of 50 h . Three rainfall regimes that correspond to different ranges of the wet Froude number ( Fr w ) are identified from the idealized simulations (with a grid size of 2 km ). The low- Fr w regime ( Fr w ≤ ∼ 0.3) is where the island circulation from thermodynamic effects is the main driver of rainfall in local afternoon. The lower the wind speed and Fr w are, the more widespread the rainfall is, as well as its amount. On the other hand, the high- Fr w regime ( Fr w ≥ ∼ 0.4) occurs when the flow of at least 12.5 m s −1 impinges on Taiwan terrain at a large angle (not parallel). This favors the flow-over scenario, and topographic rainfall production becomes dominant through mechanical uplift of unstable air. In this scenario, the faster and wetter the flow is, the heavier the rainfall on the windward slopes is, and maximum amounts typically occur at wind directions from 240–255 ∘ . Between the two regimes above, a third, mixed regime also exists. The idealized results are discussed for their applicability to the real atmosphere.
    Subject code 333 ; 511
    Language English
    Publishing date 2022-06-02
    Publishing country de
    Document type Book ; Online
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  5. Article ; Online: Wu-Mei-Wan ameliorates chronic colitis-associated intestinal fibrosis through inhibiting fibroblast activation.

    Wu, Fan / Shao, Qingqing / Hu, Meilin / Zhao, Yan / Dong, Ruolan / Fang, Ke / Xu, Lijun / Zou, Xin / Lu, Fuer / Li, Jingbin / Chen, Guang

    Journal of ethnopharmacology

    2020  Volume 252, Page(s) 112580

    Abstract: Ethnopharmacological relevance: Wu-Mei-Wan (WMW), a classic traditional Chinese herb medicine, is ...

    Abstract Ethnopharmacological relevance: Wu-Mei-Wan (WMW), a classic traditional Chinese herb medicine, is one of the most important formulations to treat digestive diseases from ancient times to the present. Previous study showed that WMW has satisfactory curative effects on experimental colitis, which motivating the application of WMW on colitis-associated complications.
    Aim of the study: Intestinal fibrosis is usually considered to be a common complication of inflammatory bowel disease (IBD), particularly Crohn's disease (CD). Currently, no effective preventive measures or medical therapies are available for that. This work was designed to evaluate the effect and related mechanism of WMW on chronic colitis-associated intestinal fibrosis mice model.
    Materials and methods: The chronic colitis-associated intestinal fibrosis mice model was established by weekly intrarectal injection of 2,4,6-trinitrobenzene sulfonic acid (TNBS). The mice survival rate, disease activity index (DAI), colon length and histological score were examined to assess the therapeutic effect of WMW. Masson's trichrome staining, hydroxyproline assay, immunohistochemical staining and western blot analysis were used to evaluate fibrosis level. Colon inflammation was determined by ELISA and immunofluorescence staining. Immunofluorescence staining was used to evaluate fibroblasts proliferation and epithelial to mesenchymal transition (EMT), and the expression of key molecules in fibrosis was analyzed by western blot.
    Results: Here we showed that WMW alleviates chronic colitis with improved survival rate, DAI, colon length and histological score. WMW inhibited the progression of intestinal fibrosis, decreased the expression of various fibrosis markers, such as α-SMA, collagen I, MMP-9 and fibronectin. In addition, WMW treatment reduced cytokines IL-6 and IFN-γ, and downregulated proinflammatory NF-κBp65 and STAT3 signaling pathways. Importantly, administration of WMW led to the inhibition of colon fibroblast proliferation and EMT, which are important mediators during fibrosis. Several key profibrotic pathways, including TGF-β/Smad and Wnt/β-catenin pathways, were downregulated by WMW treatment.
    Conclusion: Our work demonstrated that WMW can prevent intestinal fibrosis and the mechanisms involved may be related to the inhibition of colon fibroblasts activation.
    MeSH term(s) Animals ; Chronic Disease ; Colitis/complications ; Colitis/drug therapy ; Colitis/immunology ; Colitis/pathology ; Colon/drug effects ; Colon/immunology ; Colon/pathology ; Cytokines/blood ; Cytokines/immunology ; Fibroblasts/drug effects ; Fibrosis ; Male ; Medicine, Chinese Traditional ; Mice, Inbred C57BL
    Chemical Substances Cytokines
    Language English
    Publishing date 2020-01-20
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2020.112580
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  6. Article: Traditional herbal formula Wu-Mei-Wan alleviates TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation.

    Wu, Fan / Shao, Qingqing / Cheng, Zhe / Xiong, Xinyu / Fang, Ke / Zhao, Yan / Dong, Ruolan / Xu, Lijun / Lu, Fuer / Chen, Guang

    Chinese medicine

    2021  Volume 16, Issue 1, Page(s) 78

    Abstract: ... a protective effect in gut inflammation. Our recent study suggested traditional Chinese herbal formula Wu-Mei-Wan (WMW ...

    Abstract Background: Accumulating evidence indicated that necroptosis plays an essential role in the pathogenesis of inflammatory bowel disease (IBD). The O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) of necroptotic signal molecule receptor-interacting serine-threonine kinase 3 (RIPK3) was reported to exert a protective effect in gut inflammation. Our recent study suggested traditional Chinese herbal formula Wu-Mei-Wan (WMW) as an effective prescription in mouse colitis. However, the potential mechanisms are not fully understood. Considering the crucial role of necroptosis in the pathogenesis of IBD, therefore, this study was designed to explain whether the anti-colitis effect of WMW is mediated by modulating necroptosis and its related mechanisms.
    Methods: The protective effects of WMW on colitis have been determined by detecting colitis mice body weight, disease activity index (DAI), survival rate and colon length. Colonic inflammation was examined by inflammatory cells infiltration and local cytokines levels. After then, we measured the levels of necroptosis and O-GlcNAcylation. C O-immunoprecipitation experiments were used to address whether elevated O-GlcNAcylation can inhibit necroptotic signal transduction in the treatment of WMW. Finally, the key enzymes in O-GlcNAcylation: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were examined and molecular docking analysis was used to determine effective natural compounds in the regulation on OGT and OGA activities.
    Results: Our results showed that WMW significantly improved mice body weight, survival rate and colon length, decreased DAI in TNBS-induced colitis. WMW obviously alleviated colonic inflammatory responses with reduced macrophages, neutrophils infiltration and local IL-1β, IL-6, TNF-α and IFN-γ levels. It was found that WMW increased colonic O-GlcNAcylation level and inhibited the activation of RIPK1, RIPK3 and MLKL. Then, further experiments revealed that WMW enhanced OGT activity and suppressed OGA activity, thereby increasing RIPK3 O-GlcNAcylation and inhibiting the binding of RIPK3 and MLKL, which led to the inhibition of necroptosis. Additionally, docking analysis demonstrated that hesperidin, coptisine and ginsenoside Rb1 may exert a major role in the regulation on OGT and OGA activities by WMW.
    Conclusion: Our work demonstrated that WMW can alleviate TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation.
    Language English
    Publishing date 2021-08-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 2260322-0
    ISSN 1749-8546
    ISSN 1749-8546
    DOI 10.1186/s13020-021-00493-4
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  7. Article: Efficacy and Safety of Wei Bi Mei, a Chinese Herb Compound, as an Alternative to Bismuth for Eradication of

    Li, Lei / Meng, FanDong / Zhu, Shengtao / Guo, ShuiLong / Wang, YongJun / Zhao, Xin / Sun, YiLin / Zhang, Yan / Wang, QinQin / Xu, HuFeng / Zhang, ShuTian

    Evidence-based complementary and alternative medicine : eCAM

    2018  Volume 2018, Page(s) 4320219

    Abstract: ... been raised. We first assessed the efficacy and safety of Wei Bi Mei granules, which are bismuth ...

    Abstract Bismuth-containing quadruple therapy has been recommended as the first line of treatment in areas of high clarithromycin or metronidazole resistance. However, safety concerns of bismuth agents have long been raised. We first assessed the efficacy and safety of Wei Bi Mei granules, which are bismuth compounds consisting of three synthetic drugs and five medicinal herbs, compared to bismuth aluminate and colloidal bismuth subcitrate (CBS) in
    Language English
    Publishing date 2018-02-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2018/4320219
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    Zs.A 5995: Show issues Location:
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  8. Article: Wu-Mei-Wan ameliorates chronic colitis-associated intestinal fibrosis through inhibiting fibroblast activation

    Wu, Fan / Chen, Guang / Dong, Ruolan / Fang, Ke / Hu, Meilin / Li, Jingbin / Lu, Fuer / Shao, Qingqing / Xu, Lijun / Zhao, Yan / Zou, Xin

    Journal of ethnopharmacology. 2020 Apr. 24, v. 252

    2020  

    Abstract: Wu-Mei-Wan (WMW), a classic traditional Chinese herb medicine, is one of the most important ...

    Abstract Wu-Mei-Wan (WMW), a classic traditional Chinese herb medicine, is one of the most important formulations to treat digestive diseases from ancient times to the present. Previous study showed that WMW has satisfactory curative effects on experimental colitis, which motivating the application of WMW on colitis-associated complications.Intestinal fibrosis is usually considered to be a common complication of inflammatory bowel disease (IBD), particularly Crohn's disease (CD). Currently, no effective preventive measures or medical therapies are available for that. This work was designed to evaluate the effect and related mechanism of WMW on chronic colitis-associated intestinal fibrosis mice model.The chronic colitis-associated intestinal fibrosis mice model was established by weekly intrarectal injection of 2,4,6-trinitrobenzene sulfonic acid (TNBS). The mice survival rate, disease activity index (DAI), colon length and histological score were examined to assess the therapeutic effect of WMW. Masson's trichrome staining, hydroxyproline assay, immunohistochemical staining and western blot analysis were used to evaluate fibrosis level. Colon inflammation was determined by ELISA and immunofluorescence staining. Immunofluorescence staining was used to evaluate fibroblasts proliferation and epithelial to mesenchymal transition (EMT), and the expression of key molecules in fibrosis was analyzed by western blot.Here we showed that WMW alleviates chronic colitis with improved survival rate, DAI, colon length and histological score. WMW inhibited the progression of intestinal fibrosis, decreased the expression of various fibrosis markers, such as α-SMA, collagen I, MMP-9 and fibronectin. In addition, WMW treatment reduced cytokines IL-6 and IFN-γ, and downregulated proinflammatory NF-κBp65 and STAT3 signaling pathways. Importantly, administration of WMW led to the inhibition of colon fibroblast proliferation and EMT, which are important mediators during fibrosis. Several key profibrotic pathways, including TGF-β/Smad and Wnt/β-catenin pathways, were downregulated by WMW treatment.Our work demonstrated that WMW can prevent intestinal fibrosis and the mechanisms involved may be related to the inhibition of colon fibroblasts activation.
    Keywords animal models ; beta catenin ; cell proliferation ; colitis ; collagen ; colon ; Crohn disease ; enzyme-linked immunosorbent assay ; epithelium ; fibroblasts ; fibronectins ; fibrosis ; fluorescent antibody technique ; hydroxyproline ; immunohistochemistry ; inflammation ; interferon-gamma ; interleukin-6 ; mice ; Oriental traditional medicine ; signal transduction ; staining ; sulfonic acids ; survival rate ; therapeutics ; transforming growth factor beta ; Western blotting
    Language English
    Dates of publication 2020-0424
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2020.112580
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  9. Article ; Online: Traditional herbal formula Wu-Mei-Wan alleviates TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation

    Fan Wu / Qingqing Shao / Zhe Cheng / Xinyu Xiong / Ke Fang / Yan Zhao / Ruolan Dong / Lijun Xu / Fuer Lu / Guang Chen

    Chinese Medicine, Vol 16, Iss 1, Pp 1-

    2021  Volume 17

    Abstract: ... a protective effect in gut inflammation. Our recent study suggested traditional Chinese herbal formula Wu-Mei-Wan (WMW ...

    Abstract Abstract Background Accumulating evidence indicated that necroptosis plays an essential role in the pathogenesis of inflammatory bowel disease (IBD). The O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) of necroptotic signal molecule receptor-interacting serine-threonine kinase 3 (RIPK3) was reported to exert a protective effect in gut inflammation. Our recent study suggested traditional Chinese herbal formula Wu-Mei-Wan (WMW) as an effective prescription in mouse colitis. However, the potential mechanisms are not fully understood. Considering the crucial role of necroptosis in the pathogenesis of IBD, therefore, this study was designed to explain whether the anti-colitis effect of WMW is mediated by modulating necroptosis and its related mechanisms. Methods The protective effects of WMW on colitis have been determined by detecting colitis mice body weight, disease activity index (DAI), survival rate and colon length. Colonic inflammation was examined by inflammatory cells infiltration and local cytokines levels. After then, we measured the levels of necroptosis and O-GlcNAcylation. C O-immunoprecipitation experiments were used to address whether elevated O-GlcNAcylation can inhibit necroptotic signal transduction in the treatment of WMW. Finally, the key enzymes in O-GlcNAcylation: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) were examined and molecular docking analysis was used to determine effective natural compounds in the regulation on OGT and OGA activities. Results Our results showed that WMW significantly improved mice body weight, survival rate and colon length, decreased DAI in TNBS-induced colitis. WMW obviously alleviated colonic inflammatory responses with reduced macrophages, neutrophils infiltration and local IL-1β, IL-6, TNF-α and IFN-γ levels. It was found that WMW increased colonic O-GlcNAcylation level and inhibited the activation of RIPK1, RIPK3 and MLKL. Then, further experiments revealed that WMW enhanced OGT activity and suppressed OGA activity, thereby increasing RIPK3 ...
    Keywords Wu-Mei-Wan ; Colitis ; Necroptosis ; O-GlcNAcylation ; RIPK3 ; Other systems of medicine ; RZ201-999
    Language English
    Publishing date 2021-08-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
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  10. Article: Wu-Mei-Wan Reduces Insulin Resistance via Inhibition of NLRP3 Inflammasome Activation in HepG2 Cells.

    Yang, Xueping / Li, Lingli / Fang, Ke / Dong, Ruolan / Li, Jingbin / Zhao, Yan / Dong, Hui / Yi, Ping / Huang, Zhaoyi / Chen, Guang / Lu, Fuer

    Evidence-based complementary and alternative medicine : eCAM

    2017  Volume 2017, Page(s) 7283241

    Abstract: Wu-Mei-Wan (WMW) is a Chinese herbal formula used to treat type 2 diabetes. In this study, we aimed ...

    Abstract Wu-Mei-Wan (WMW) is a Chinese herbal formula used to treat type 2 diabetes. In this study, we aimed to explore the effects and mechanisms of WMW on insulin resistance in HepG2 cells. HepG2 cells were pretreated with palmitate (0.25 mM) to impair the insulin signaling pathway. Then, they were treated with different doses of WMW-containing medicated serum and stimulated with 100 nM insulin. Results showed that palmitate could reduce the glucose consumption rate in HepG2 cells and impair insulin signaling related to phosphorylation of insulin receptor (IR) and insulin receptor substrate-1 (IRS-1), thereby regulating the downstream signaling pathways. However, medicated serum of WMW restored impaired insulin signaling, upregulated the expression of phospho-IR (pIR), phosphatidylinositol 3-kinase p85 subunit, phosphoprotein kinase B, and glucose transporter 4, and decreased IRS serine phosphorylation. In addition, it decreased the expression of interleukin-1
    Language English
    Publishing date 2017-08-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2017/7283241
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