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  1. Article ; Online: Investigating Mitochondrial Ca

    Bhosale, Gauri / Duchen, Michael R

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2497, Page(s) 325–332

    Abstract: Mitochondrial ... ...

    Abstract Mitochondrial Ca
    MeSH term(s) Calcium/metabolism ; Calcium Signaling ; Cytosol/metabolism ; Fluorescent Dyes/metabolism ; Mitochondria/metabolism
    Chemical Substances Fluorescent Dyes ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2022-06-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2309-1_23
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  2. Article ; Online: Monitoring Mitochondrial Membrane Potential in Live Cells Using Time-Lapse Fluorescence Imaging.

    Valdebenito, Gabriel Esteban / Duchen, Michael R

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2497, Page(s) 319–324

    Abstract: The mitochondrial membrane potential ( ... ...

    Abstract The mitochondrial membrane potential (ΔΨ
    MeSH term(s) Cells, Cultured ; Membrane Potential, Mitochondrial ; Mitochondria/metabolism ; Optical Imaging ; Time-Lapse Imaging
    Language English
    Publishing date 2022-06-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2309-1_22
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  3. Article ; Online: Analysis of Organization and Activity of Mitochondrial Respiratory Chain Complexes in Primary Fibroblasts Using Blue Native PAGE.

    Singh, Kritarth / Duchen, Michael R

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2497, Page(s) 339–348

    Abstract: Blue Native polyacrylamide gel electrophoresis (BN-PAGE) is a well-established technique for the isolation and separation of mitochondrial membrane protein complexes in a native conformation with high resolution. In combination with histochemical ... ...

    Abstract Blue Native polyacrylamide gel electrophoresis (BN-PAGE) is a well-established technique for the isolation and separation of mitochondrial membrane protein complexes in a native conformation with high resolution. In combination with histochemical staining methods, BN-PAGE has been successfully used as clinical diagnostic tool for the detection of oxidative phosphorylation (OXPHOS) defects from small tissue biopsies from patients with primary mitochondrial disease. However, its application to patient-derived primary fibroblasts is difficult due to limited proliferation and high background staining. Here, we describe a rapid and convenient method to analyze the organization and activity of OXPHOS complexes from cultured skin fibroblasts.
    MeSH term(s) Electron Transport ; Electrophoresis, Polyacrylamide Gel ; Fibroblasts ; Humans ; Mitochondrial Membranes ; Native Polyacrylamide Gel Electrophoresis/methods
    Language English
    Publishing date 2022-06-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2309-1_25
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  4. Article ; Online: A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX.

    Chung, Chih-Yao / Duchen, Michael R

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2497, Page(s) 333–337

    Abstract: Intracellular reactive oxygen species (ROS) act as an important signaling transductor in cells, regulating almost every aspect of cell biology. Measurements of ROS production thus, offer links between oxidative stress and cell pathophysiology. Here, we ... ...

    Abstract Intracellular reactive oxygen species (ROS) act as an important signaling transductor in cells, regulating almost every aspect of cell biology. Measurements of ROS production thus, offer links between oxidative stress and cell pathophysiology. Here, we describe a simple screening assay in intact adherent cells by fluorescence microplate readers, using dihydroethidium (DHE) and MitoSOX to measure cytosolic superoxide and mitochondrial superoxide production, respectively. This assay enables a quick and reliable assessment of ROS generation in a well-controlled environment.
    MeSH term(s) Ethidium/analogs & derivatives ; Oxidative Stress ; Phenanthridines ; Reactive Oxygen Species/analysis ; Superoxides/analysis
    Chemical Substances 5-(6'-triphenylphosphoniumhexyl)-5,6-dihydro-6-phenyl-3,8-phenanthridinediammine ; Phenanthridines ; Reactive Oxygen Species ; dihydroethidium (104821-25-2) ; Superoxides (11062-77-4) ; Ethidium (EN464416SI)
    Language English
    Publishing date 2022-06-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2309-1_24
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  5. Article ; Online: The mitochondrial ATP synthase as an ATP consumer-a surprising therapeutic target.

    Valdebenito, Gabriel E / Chacko, Anitta R / Duchen, Michael R

    The EMBO journal

    2023  Volume 42, Issue 10, Page(s) e114141

    Abstract: The mitochondrial ... ...

    Abstract The mitochondrial F
    MeSH term(s) Mitochondrial Proton-Translocating ATPases/metabolism ; Adenosine Triphosphate ; Protons ; Mitochondria/metabolism
    Chemical Substances Mitochondrial Proton-Translocating ATPases (EC 3.6.3.-) ; Adenosine Triphosphate (8L70Q75FXE) ; Protons
    Language English
    Publishing date 2023-04-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 586044-1
    ISSN 1460-2075 ; 0261-4189
    ISSN (online) 1460-2075
    ISSN 0261-4189
    DOI 10.15252/embj.2023114141
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    Zs.A 1808: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  6. Article ; Online: NAD(P)H binding configurations revealed by time-resolved fluorescence and two-photon absorption.

    Blacker, Thomas S / Duchen, Michael R / Bain, Angus J

    Biophysical journal

    2023  Volume 122, Issue 7, Page(s) 1240–1253

    Abstract: NADH and NADPH play key roles in the regulation of metabolism. Their endogenous fluorescence is sensitive to enzyme binding, allowing changes in cellular metabolic state to be determined using fluorescence lifetime imaging microscopy (FLIM). However, to ... ...

    Abstract NADH and NADPH play key roles in the regulation of metabolism. Their endogenous fluorescence is sensitive to enzyme binding, allowing changes in cellular metabolic state to be determined using fluorescence lifetime imaging microscopy (FLIM). However, to fully uncover the underlying biochemistry, the relationships between their fluorescence and binding dynamics require greater understanding. Here we accomplish this through time- and polarization-resolved fluorescence and polarized two-photon absorption measurements. Two lifetimes result from binding of both NADH to lactate dehydrogenase and NADPH to isocitrate dehydrogenase. The composite fluorescence anisotropy indicates the shorter (1.3-1.6 ns) decay component to be accompanied by local motion of the nicotinamide ring, pointing to attachment solely via the adenine moiety. For the longer lifetime (3.2-4.4 ns), the nicotinamide conformational freedom is found to be fully restricted. As full and partial nicotinamide binding are recognized steps in dehydrogenase catalysis, our results unify photophysical, structural, and functional aspects of NADH and NADPH binding and clarify the biochemical processes that underlie their contrasting intracellular lifetimes.
    MeSH term(s) NAD/chemistry ; NAD/metabolism ; NADP ; Fluorescence ; Niacinamide ; Catalysis
    Chemical Substances NAD (0U46U6E8UK) ; NADP (53-59-8) ; Niacinamide (25X51I8RD4)
    Language English
    Publishing date 2023-02-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218078-9
    ISSN 1542-0086 ; 0006-3495
    ISSN (online) 1542-0086
    ISSN 0006-3495
    DOI 10.1016/j.bpj.2023.02.014
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    Zs.A 235: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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    Zs.MO 2: Show issues

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  7. Book ; Online ; E-Book: Mitochondrial dysfunction in neurodegenerative disorders

    Reeve, Amy Katherine / Simcox, Eve M. / Duchen, Michael R. / Turnbull, Doug M.

    2016  

    Author's details Amy K. Reeve, Eve M. Simcox, Michael R. Duchen, Doug M. Turnbull editors
    Language English
    Size 1 Online-Ressource (x, 380 Seiten), Illustrationen
    Edition Second edition
    Publisher Springer
    Publishing place Cham
    Publishing country Switzerland
    Document type Book ; Online ; E-Book
    Remark Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT019057223
    ISBN 978-3-319-28637-2 ; 9783319286358 ; 3-319-28637-4 ; 3319286358
    DOI 10.1007/978-3-319-28637-2
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  8. Article ; Online: Assessing the Redox Status of Mitochondria Through the NADH/FAD

    Chi, Haoyu / Bhosale, Gauri / Duchen, Michael R

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2497, Page(s) 313–318

    Abstract: This section aims to describe the measurement of NADH and ... ...

    Abstract This section aims to describe the measurement of NADH and FAD
    MeSH term(s) Flavin-Adenine Dinucleotide/metabolism ; Microscopy, Fluorescence ; Mitochondria/metabolism ; NAD/metabolism ; Oxidation-Reduction
    Chemical Substances NAD (0U46U6E8UK) ; Flavin-Adenine Dinucleotide (146-14-5)
    Language English
    Publishing date 2022-06-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2309-1_21
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  9. Article ; Online: Investigating the Mitochondrial Permeability Transition Pore in Disease Phenotypes and Drug Screening.

    Bhosale, Gauri / Duchen, Michael R

    Current protocols in pharmacology

    2019  Volume 85, Issue 1, Page(s) e59

    Abstract: Mitochondria act as 'sinks' for ... ...

    Abstract Mitochondria act as 'sinks' for Ca
    MeSH term(s) Animals ; Calcium/metabolism ; Cryopreservation ; Drug Evaluation, Preclinical/methods ; Immunoblotting ; Membrane Potential, Mitochondrial ; Mice ; Mitochondria, Liver ; Mitochondrial Membrane Transport Proteins/metabolism ; Mitochondrial Permeability Transition Pore ; Oxygen Consumption ; Phenotype
    Chemical Substances Mitochondrial Membrane Transport Proteins ; Mitochondrial Permeability Transition Pore ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2019-05-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2179074-7
    ISSN 1934-8290 ; 1934-8282
    ISSN (online) 1934-8290
    ISSN 1934-8282
    DOI 10.1002/cpph.59
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  10. Article ; Online: Excitotoxicity Revisited: Mitochondria on the Verge of a Nervous Breakdown.

    Plotegher, Nicoletta / Filadi, Riccardo / Pizzo, Paola / Duchen, Michael R

    Trends in neurosciences

    2021  Volume 44, Issue 5, Page(s) 342–351

    Abstract: Excitotoxicity is likely to occur in pathological scenarios in which mitochondrial function is already compromised, shaping neuronal responses to glutamate. In fact, mitochondria sustain cell bioenergetics, tune intracellular ... ...

    Abstract Excitotoxicity is likely to occur in pathological scenarios in which mitochondrial function is already compromised, shaping neuronal responses to glutamate. In fact, mitochondria sustain cell bioenergetics, tune intracellular Ca
    MeSH term(s) Calcium/metabolism ; Energy Metabolism ; Glutamic Acid/metabolism ; Humans ; Mitochondria/metabolism ; Neurons/metabolism
    Chemical Substances Glutamic Acid (3KX376GY7L) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2021-02-16
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 282488-7
    ISSN 1878-108X ; 0378-5912 ; 0166-2236
    ISSN (online) 1878-108X
    ISSN 0378-5912 ; 0166-2236
    DOI 10.1016/j.tins.2021.01.001
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    Zs.A 1442: Show issues Location:
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    Zs.MG 53: Show issues

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