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Article ; Online: Dearomative (4 + 3) Cycloaddition Reactions of 3-Alkenylindoles and 3-Alkenylpyrroles to Afford Cyclohepta[

Taenzler, Ferdinand / Xu, Jiasu / Athe, Sudhakar / Rawal, Viresh H

Organic letters

2022 Volume 24, Issue 44, Page(s) 8109–8114

Abstract: The dearomative (4 + 3) cycloaddition reactions of 3-alkenylindoles with in situ-generated oxyallyl cations furnish cyclohepta[ ...

Abstract	The dearomative (4 + 3) cycloaddition reactions of 3-alkenylindoles with in situ-generated oxyallyl cations furnish cyclohepta[
MeSH term(s)	Cycloaddition Reaction ; Pyrroles ; Indoles ; Stereoisomerism ; Cations
Chemical Substances	Pyrroles ; Indoles ; Cations
Language	English
Publishing date	2022-10-31
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
ISSN	1523-7052
ISSN (online)	1523-7052
DOI	10.1021/acs.orglett.2c02983
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Codetection of Proteins and RNAs on Extracellular Vesicles for Pancreatic Cancer Early Diagnosis.

He, Jie / Long, Jiang / Zhai, Chunhui / Xu, Jiasu / Bao, Kaiwen / Su, Wenqiong / Jiang, Lai / Shen, Guangxia / Ding, Xianting

Analytical chemistry

2024

Abstract: Tumor-derived extracellular vesicles (EVs) carry tumor-specific proteins and RNAs, thus becoming prevalent targets for early cancer diagnosis. However, low expression of EV cargos and insufficient diagnostic power of individual biomarkers hindered EVs ... ...

Abstract	Tumor-derived extracellular vesicles (EVs) carry tumor-specific proteins and RNAs, thus becoming prevalent targets for early cancer diagnosis. However, low expression of EV cargos and insufficient diagnostic power of individual biomarkers hindered EVs application in clinical practice. Herein, we propose a multiplex Codetection platform of proteins and RNAs (Co-PAR) for EVs. Co-PAR adopted a pair of antibody-DNA probes to recognize the same target protein, which in turn formed a double-stranded DNA. Thus, the target protein could be quantified by detecting the double-stranded DNA via qPCR. Meanwhile, qRT-PCR simultaneously quantified the target RNAs. Thus, with a regular qPCR instrument, Co-PAR enabled the codetection of multiplex proteins and RNAs, with the sensitivity of 10
Language	English
Publishing date	2024-04-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c05858
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Article ; Online: GPR Clutter Removal Based on Weighted Nuclear Norm Minimization for Nonparallel Cases.

Liu, Li / Song, Chenyan / Wu, Zezhou / Xu, Hang / Li, Jingxia / Wang, Bingjie / Li, Jiasu

Sensors (Basel, Switzerland)

2023 Volume 23, Issue 11

Abstract: Ground-penetrating radar (GPR) is an effective geophysical electromagnetic method for underground target detection. However, the target response is usually overwhelmed by strong clutter, thus damaging the detection performance. To account for the ... ...

Abstract	Ground-penetrating radar (GPR) is an effective geophysical electromagnetic method for underground target detection. However, the target response is usually overwhelmed by strong clutter, thus damaging the detection performance. To account for the nonparallel case of the antennas and the ground surface, a novel GPR clutter-removal method based on weighted nuclear norm minimization (WNNM) is proposed, which decomposes the B-scan image into a low-rank clutter matrix and a sparse target matrix by using a non-convex weighted nuclear norm and assigning different weights to different singular values. The WNNM method's performance is evaluated using both numerical simulations and experiments with real GPR systems. Comparative analysis with the commonly used state-of-the-art clutter removal methods is also conducted in terms of the peak signal-to-noise ratio (PSNR) and the improvement factor (IF). The visualization and quantitative results demonstrate that the proposed method outperforms the others in the nonparallel case. Moreover, it is about five times faster than the RPCA, which is beneficial for practical applications.
Language	English
Publishing date	2023-05-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s23115078
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Total Synthesis of (-)-Ambiguine P.

Xu, Jiasu / Rawal, Viresh H

Journal of the American Chemical Society

2019 Volume 141, Issue 12, Page(s) 4820–4823

Abstract: Described is a concise total synthesis of (-)-ambiguine P, a cycloheptane-containing member of the hapalindole alkaloids. The challenging pentacyclic framework of the natural product was assembled rapidly via a [4 + 3] cycloaddition reaction-inspired ... ...

Abstract	Described is a concise total synthesis of (-)-ambiguine P, a cycloheptane-containing member of the hapalindole alkaloids. The challenging pentacyclic framework of the natural product was assembled rapidly via a [4 + 3] cycloaddition reaction-inspired strategy, and the tertiary hydroxy group was introduced by an NBS-mediated bromination-nucleophilic substitution sequence.
Language	English
Publishing date	2019-03-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.9b01739
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Article ; Online: SpiDe-Sr: blind super-resolution network for precise cell segmentation and clustering in spatial proteomics imaging.

Chen, Rui / Xu, Jiasu / Wang, Boqian / Ding, Yi / Abdulla, Aynur / Li, Yiyang / Jiang, Lai / Ding, Xianting

Nature communications

2024 Volume 15, Issue 1, Page(s) 2708

Abstract: Spatial proteomics elucidates cellular biochemical changes with unprecedented topological level. Imaging mass cytometry (IMC) is a high-dimensional single-cell resolution platform for targeted spatial proteomics. However, the precision of subsequent ... ...

Abstract	Spatial proteomics elucidates cellular biochemical changes with unprecedented topological level. Imaging mass cytometry (IMC) is a high-dimensional single-cell resolution platform for targeted spatial proteomics. However, the precision of subsequent clinical analysis is constrained by imaging noise and resolution. Here, we propose SpiDe-Sr, a super-resolution network embedded with a denoising module for IMC spatial resolution enhancement. SpiDe-Sr effectively resists noise and improves resolution by 4 times. We demonstrate SpiDe-Sr respectively with cells, mouse and human tissues, resulting 18.95%/27.27%/21.16% increase in peak signal-to-noise ratio and 15.95%/31.63%/15.52% increase in cell extraction accuracy. We further apply SpiDe-Sr to study the tumor microenvironment of a 20-patient clinical breast cancer cohort with 269,556 single cells, and discover the invasion of Gram-negative bacteria is positively correlated with carcinogenesis markers and negatively correlated with immunological markers. Additionally, SpiDe-Sr is also compatible with fluorescence microscopy imaging, suggesting SpiDe-Sr an alternative tool for microscopy image super-resolution.
MeSH term(s)	Humans ; Animals ; Mice ; Female ; Proteomics ; Diagnostic Imaging ; Signal-To-Noise Ratio ; Breast Neoplasms/diagnostic imaging ; Cluster Analysis ; Image Processing, Computer-Assisted/methods ; Tumor Microenvironment
Language	English
Publishing date	2024-03-28
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-46989-z
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Article ; Online: Multiplex Protein Profiling by Low-Signal-Loss Single-Cell Western Blotting with Fluorescent-Quenching Aptamers.

Xu, Li / Xie, Haiyang / Wang, Boqian / Zhu, Zijian / Jiang, Hui / Duan, Xiaoqian / Deng, Shuxin / Xu, Jiasu / Jiang, Lai / Ding, Xianting

Analytical chemistry

2023 Volume 95, Issue 30, Page(s) 11399–11409

Abstract: Single-cell western blotting (scWB) is a prevalent technique for high-resolution protein analysis on low-abundance cell samples. However, the extensive signal loss during repeated antibody stripping precludes multiplex protein detection. Herein, we ... ...

Abstract	Single-cell western blotting (scWB) is a prevalent technique for high-resolution protein analysis on low-abundance cell samples. However, the extensive signal loss during repeated antibody stripping precludes multiplex protein detection. Herein, we introduce
MeSH term(s)	Aptamers, Nucleotide ; Fluorescent Dyes ; Antibodies ; Proteins ; Blotting, Western
Chemical Substances	Aptamers, Nucleotide ; Fluorescent Dyes ; Antibodies ; Proteins
Language	English
Publishing date	2023-07-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c01577
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ab Jg. 2022: Lesesaal (EG)

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Article: Total Synthesis of (−)-Ambiguine P

Xu, Jiasu / Viresh H. Rawal

Journal of the American Chemical Society. 2019 Mar. 11, v. 141, no. 12

2019

Abstract: Described is a concise total synthesis of (−)-ambiguine P, a cycloheptane-containing member of the hapalindole alkaloids. The challenging pentacyclic framework of the natural product was assembled rapidly via a [4 + 3] cycloaddition reaction-inspired ... ...

Abstract	Described is a concise total synthesis of (−)-ambiguine P, a cycloheptane-containing member of the hapalindole alkaloids. The challenging pentacyclic framework of the natural product was assembled rapidly via a [4 + 3] cycloaddition reaction-inspired strategy, and the tertiary hydroxy group was introduced by an NBS-mediated bromination–nucleophilic substitution sequence.
Keywords	alkaloids ; cycloaddition reactions
Language	English
Dates of publication	2019-0311
Size	p. 4820-4823.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/jacs.9b01739
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Photosensitive Hydrogel with Temperature-Controlled Reversible Nano-Apertures for Single-Cell Protein Analysis.

Xie, Haiyang / Guo, Wenke / Jiang, Hui / Zhang, Ting / Zhao, Lei / Hu, Jinjuan / Gao, Shuxin / Song, Sunfengda / Xu, Jiasu / Xu, Li / Sun, Xinyi / Ding, Yi / Jiang, Lai / Ding, Xianting

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

2024 , Page(s) e2308569

Abstract: Single cell western blot (scWB) is one of the most important methods for cellular heterogeneity profiling. However, current scWB based on conventional photoactive polyacrylamide hydrogel material suffers from the tradeoff between in-gel probing and ... ...

Abstract	Single cell western blot (scWB) is one of the most important methods for cellular heterogeneity profiling. However, current scWB based on conventional photoactive polyacrylamide hydrogel material suffers from the tradeoff between in-gel probing and separation resolution. Here, a highly sensitive temperature-controlled single-cell western blotting (tc-scWB) method is introduced, which is based on a thermo/photo-dualistic-sensitive polyacrylamide hydrogel, namely acrylic acid-functionalized graphene oxide (AFGO) assisted, N-isopropylacrylamide modified polyacrylamide (ANP) hydrogel. The ANP hydrogel is contracted at high-temperature to constrain protein band diffusion during microchip electrophoretic separation, while the gel aperture is expanded under low-temperature for better antibody penetration into the hydrogel. The tc-scWB method enables the separation and profiling of small-molecule-weight proteins with highly crosslinked gel (12% T) in SDS-PAGE. The tc-scWB is demonstrated on three metabolic and ER stress-specific proteins (CHOP, MDH2 and FH) in four pancreatic cell subtypes, revealing the expression of key enzymes in the Krebs cycle is upregulated with enhanced ER stress. It is found that ER stress can regulate crucial enzyme (MDH2 and FH) activities of metabolic cascade in cancer cells, boosting aerobic respiration to attenuate the Warburg effect and promote cell apoptosis. The tc-scWB is a general toolbox for the analysis of low-abundance small-molecular functional proteins at the single-cell level.
Language	English
Publishing date	2024-03-14
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2808093-2
ISSN	2198-3844 ; 2198-3844
ISSN (online)	2198-3844
ISSN	2198-3844
DOI	10.1002/advs.202308569
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Stoichiometric reactions and catalytic dehydrogenations of amine-boranes with calcium aryloxide.

Zheng, Xizhou / Huang, Jiasu / Yao, Yingming / Xu, Xin

Chemical communications (Cambridge, England)

2019 Volume 55, Issue 62, Page(s) 9152–9155

Abstract: Catalysis with the main-group metal calcium conventionally relies on σ-bond metathesis to initiate the reactions. Here, we report a calcium aryloxide based on the tridentate β-diketiminato ligand, which shows transition-metal-like reactivity with ... ...

Abstract	Catalysis with the main-group metal calcium conventionally relies on σ-bond metathesis to initiate the reactions. Here, we report a calcium aryloxide based on the tridentate β-diketiminato ligand, which shows transition-metal-like reactivity with dialkylamine-boranes H
Language	English
Publishing date	2019-07-15
Publishing country	England
Document type	Journal Article
ZDB-ID	1472881-3
ISSN	1364-548X ; 1359-7345 ; 0009-241X
ISSN (online)	1364-548X
ISSN	1359-7345 ; 0009-241X
DOI	10.1039/c9cc04698d
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Multiplex Protein Profiling by Low-Signal-Loss Single-Cell Western Blotting with Fluorescent-Quenching Aptamers

Xu, Li / Xie, Haiyang / Wang, Boqian / Zhu, Zijian / Jiang, Hui / Duan, Xiaoqian / Deng, Shuxin / Xu, Jiasu / Jiang, Lai / Ding, Xianting

Analytical Chemistry. 2023 July 17, v. 95, no. 30 p.11399-11409

2023

Abstract	Single-cell western blotting (scWB) is a prevalent technique for high-resolution protein analysis on low-abundance cell samples. However, the extensive signal loss during repeated antibody stripping precludes multiplex protein detection. Herein, we introduce Fluorescent-quenching Aptamer-based Single-cell Western Blotting (FAS-WB) for multiplex protein detection at single-cell resolution. The minimal size of aptamer probes allows rapid in-gel penetration, diffusion, and elution. Meanwhile, the fluorophore-tagged aptamers, coordinated with complementary quenching strands, avoid the massive signal loss conventionally caused by antibody stripping during repeated staining. Such a strategy also facilitates multiplex protein analysis with a limited number of fluorescent tags. We demonstrated FAS-WB for co-imaging four biomarker proteins (EpCAM, PTK7, HER2, CA125) at single-cell resolution with lower signal loss and enhanced signal-to-noise ratio compared to conventional antibody-based scWB. Being more time-saving (less than 25 min per cycle) and economical (1/1000 cost of conventional antibody probes), FAS-WB offers a highly efficient platform for profiling multiplex proteins at single-cell resolution.
Keywords	analytical chemistry ; antibodies ; biomarkers ; fluorescence ; oligonucleotides ; signal-to-noise ratio
Language	English
Dates of publication	2023-0717
Size	p. 11399-11409.
Publishing place	American Chemical Society
Document type	Article ; Online
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c01577
Database	NAL-Catalogue (AGRICOLA)

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