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Article ; Online: The role of ferroptosis as a regulator of oxidative stress in the pathogenesis of ischemic stroke.

Delgado-Martín, Susana / Martínez-Ruiz, Antonio

2024

Abstract: Ferroptosis is a unique form of cell death that was first described in 2012 and plays a significant role in various diseases, including neurodegenerative conditions. It depends on a dysregulation of cellular iron metabolism, which increases free, redox- ... ...

Abstract	Ferroptosis is a unique form of cell death that was first described in 2012 and plays a significant role in various diseases, including neurodegenerative conditions. It depends on a dysregulation of cellular iron metabolism, which increases free, redox-active, iron that can trigger Fenton reactions, generating hydroxyl radicals that damage cells through oxidative stress and lipid peroxidation. Lipid peroxides, resulting mainly from unsaturated fatty acids, damage cells by disrupting membrane integrity and propagating cell death signals. Moreover, lipid peroxide degradation products can further affect cellular components such as DNA, proteins, and amines. In ischemic stroke, where blood flow to the brain is restricted, there is increased iron absorption, oxidative stress, and compromised blood-brain barrier integrity. Imbalances in iron-transport and -storage proteins increase lipid oxidation and contribute to neuronal damage, thus pointing to the possibility of brain cells, especially neurons, dying from ferroptosis. Here, we review the evidence showing a role of ferroptosis in ischemic stroke, both in recent studies directly assessing this type of cell death, as well as in previous studies showing evidence that can now be revisited with our new knowledge on ferroptosis mechanisms. We also review the efforts made to target ferroptosis in ischemic stroke as a possible treatment to mitigate cellular damage and death.
Language	English
Publishing date	2024-04-26
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	212746-5
ISSN	1873-3468 ; 0014-5793
ISSN (online)	1873-3468
ISSN	0014-5793
DOI	10.1002/1873-3468.14894
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Article: Regulation of Ras Signaling by S-Nitrosylation.

Simão, Sónia / Agostinho, Rafaela Ribeiro / Martínez-Ruiz, Antonio / Araújo, Inês Maria

Antioxidants (Basel, Switzerland)

2023 Volume 12, Issue 8

Abstract: Ras are a family of small GTPases that function as signal transduction mediators and are involved in cell proliferation, migration, differentiation and survival. The significance of Ras is further evidenced by the fact that Ras genes are among the most ... ...

Abstract	Ras are a family of small GTPases that function as signal transduction mediators and are involved in cell proliferation, migration, differentiation and survival. The significance of Ras is further evidenced by the fact that Ras genes are among the most mutated oncogenes in different types of cancers. After translation, Ras proteins can be targets of post-translational modifications (PTM), which can alter the intracellular dynamics of the protein. In this review, we will focus on how S-nitrosylation of Ras affects the way these proteins interact with membranes, its cellular localization, and its activity. S-Nitrosylation occurs when a nitrosyl moiety of nitric oxide (NO) is covalently attached to a thiol group of a cysteine residue in a target protein. In Ras, the conserved Cys118 is the most surface-exposed Cys and the preferable residue for NO action, leading to the initiation of transduction events. Ras transduces the mitogen-activated protein kinases (MAPK), the phosphoinositide-3 kinase (PI3K) and the RalGEF cellular pathways. S-Nitrosylation of elements of the RalGEF cascade remains to be identified. On the contrary, it is well established that several components of the MAPK and PI3K pathways, as well as different proteins associated with these cascades, can be modified by S-nitrosylation. Overall, this review presents a better understanding of Ras S-nitrosylation, increasing the knowledge on the dynamics of these proteins in the presence of NO and the underlying implications in cellular signaling.
Language	English
Publishing date	2023-08-04
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox12081562
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Article ; Online: Post-Translational Nitric Oxide-Dependent Modifications In Immune System.

Martínez-Ruiz, Antonio

Redox biology

2015 Volume 5, Page(s) 418–419

Abstract: Nitric oxide non classical signalling is exerted through a series of covalent protein post-translational modifications, which include modification of cysteine residues by S-nitrosylation and S-glutathionylation. A key process in adaptive immunity is the ... ...

Abstract	Nitric oxide non classical signalling is exerted through a series of covalent protein post-translational modifications, which include modification of cysteine residues by S-nitrosylation and S-glutathionylation. A key process in adaptive immunity is the immune synapse that tightly couples T cells with antigen presenting cells, triggering antigen recognition by T cells. In this highly regulated process, we have shown that eNOS is activated, inducing protein S-nitrosylation. While both N-Ras and K-Ras are present in T cells, only N-Ras, which colocalizes in the Golgi with eNOS, is S-nitrosylated and activated during the immune synapse, providing an example of short-range selectivity of NO signalling through S-nitrosylation. We have developed proteomic methods to detect S-nitrosylation and reversible cysteine oxidations. We have applied them to detecting S-nitrosylated proteins in macrophage activation, highlighting the role of denitrosylase mechanism, particularly the thioredoxin pathway, in protecting macrophages from self-modification. We have also applied these proteomic methods to studying protein modification in acute hypoxia. In endothelial cells, there is an increase in cysteine oxidation in several proteins that can mediate acute responses to hypoxia prior to the activation of the HIF pathway, and we are currently studying in more detail the role of protein S-nitrosylation. We have also recently shown that acute hypoxia produces a superoxide burst in cells, which can be converted in an oxidative signal through protein cysteine modification, and we are unraveling the molecular mechanisms producing this superoxide burst in mitochondria.
Language	English
Publishing date	2015-08
Publishing country	Netherlands
Document type	Journal Article
ISSN	2213-2317
ISSN (online)	2213-2317
DOI	10.1016/j.redox.2015.09.027
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online: Compilado de proyectos de investigación científica empresarial : semana de la ciencia, la tecnología, la innovación y el emprendimiento

Mendoza Sánchez, Bernardo / Linero Meléndez, Carlos Manuel / Cárdenas Cantillo, Ingrid / Silva Tapias, Leonel / Ruiz Sánchez, Edier Alejandro / Villamil Villadiego, Graciela Esther / Guzman, Kedyn / Callejas Porto, Marcela / Calabria López, Marlon Antonio / Molina, William / Martínez Espeleta, Gustavo Rafael / Martínez T, Juan Carlos / Martínez Torres, Diana Carolina / Redondo Ochoa, Wendy / Towers, Walter / Galiano, Jossi / Martínez Juvene, Johanna / Betancourt Rodríguez, Libnazaret / Cortés Bracho, Oriana /

Martínez Torres, Juan Carlos / Martínez Torres, Juan Carlos

2023

Keywords	YQV ; Marketing ; Research ; Technological innovations ; Social entrepreneurship ; Rural development projects ; Industrial project management ; Small and medium-sized companies
Language	Spanish
Size	1 electronic resource (21 pages pages)
Publisher	Corporación Universitaria Americana
Publishing place	Barranquilla
Document type	Book ; Online
Note	Spanish
HBZ-ID	HT030380835
ISBN	9789585169531 ; 9585169533
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: Inhibition of ALK3-mediated signalling pathway protects against acetaminophen-induced liver injury.

Marañón, Patricia / Rey, Esther / Isaza, Stephania C / Wu, Hanghang / Rada, Patricia / Choya-Foces, Carmen / Martínez-Ruiz, Antonio / Martín, María Ángeles / Ramos, Sonia / García-Monzón, Carmelo / Cubero, Francisco Javier / Valverde, Ángela M / González-Rodríguez, Águeda

Redox biology

2024 Volume 71, Page(s) 103088

Abstract: Acetaminophen (APAP)-induced liver injury is one of the most prevalent causes of acute liver failure (ALF). We assessed the role of the bone morphogenetic protein (BMP) type I receptors ALK2 and ALK3 in APAP-induced hepatotoxicity. The molecular ... ...

Abstract	Acetaminophen (APAP)-induced liver injury is one of the most prevalent causes of acute liver failure (ALF). We assessed the role of the bone morphogenetic protein (BMP) type I receptors ALK2 and ALK3 in APAP-induced hepatotoxicity. The molecular mechanisms that regulate the balance between cell death and survival and the response to oxidative stress induced by APAP was assessed in cultured human hepatocyte-derived (Huh7) cells treated with pharmacological inhibitors of ALK receptors and with modulated expression of ALK2 or ALK3 by lentiviral infection, and in a mouse model of APAP-induced hepatotoxicity. Inhibition of ALK3 signalling with the pharmacological inhibitor DMH2, or by silencing of ALK3, showed a decreased cell death both by necrosis and apoptosis after APAP treatment. Also, upon APAP challenge, ROS generation was ameliorated and, thus, ROS-mediated JNK and P38 MAPK phosphorylation was reduced in ALK3-inhibited cells compared to control cells. These results were also observed in an experimental model of APAP-induced ALF in which post-treatment with DMH2 after APAP administration significantly reduced liver tissue damage, apoptosis and oxidative stress. This study shows the protective effect of ALK3 receptor inhibition against APAP-induced hepatotoxicity. Furthermore, findings obtained from the animal model suggest that BMP signalling might be a new pharmacological target for the treatment of ALF.
MeSH term(s)	Mice ; Animals ; Humans ; Acetaminophen/adverse effects ; Reactive Oxygen Species/metabolism ; Chemical and Drug Induced Liver Injury, Chronic/metabolism ; Liver/metabolism ; Hepatocytes/metabolism ; Oxidative Stress ; Chemical and Drug Induced Liver Injury/genetics ; Chemical and Drug Induced Liver Injury/metabolism ; Mice, Inbred C57BL ; Morpholines
Chemical Substances	Acetaminophen (362O9ITL9D) ; Reactive Oxygen Species ; DMH2 ; Morpholines
Language	English
Publishing date	2024-02-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2701011-9
ISSN	2213-2317 ; 2213-2317
ISSN (online)	2213-2317
ISSN	2213-2317
DOI	10.1016/j.redox.2024.103088
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Article ; Online: Regulation of Ras Signaling by S-Nitrosylation

Simão, Sónia / Agostinho, Rafaela Ribeiro / Martínez-Ruiz, Antonio / Araújo, Inês Maria

Antioxidants. 2023 Aug. 04, v. 12, no. 8

2023

Abstract	Ras are a family of small GTPases that function as signal transduction mediators and are involved in cell proliferation, migration, differentiation and survival. The significance of Ras is further evidenced by the fact that Ras genes are among the most mutated oncogenes in different types of cancers. After translation, Ras proteins can be targets of post-translational modifications (PTM), which can alter the intracellular dynamics of the protein. In this review, we will focus on how S-nitrosylation of Ras affects the way these proteins interact with membranes, its cellular localization, and its activity. S-Nitrosylation occurs when a nitrosyl moiety of nitric oxide (NO) is covalently attached to a thiol group of a cysteine residue in a target protein. In Ras, the conserved Cys118 is the most surface-exposed Cys and the preferable residue for NO action, leading to the initiation of transduction events. Ras transduces the mitogen-activated protein kinases (MAPK), the phosphoinositide-3 kinase (PI3K) and the RalGEF cellular pathways. S-Nitrosylation of elements of the RalGEF cascade remains to be identified. On the contrary, it is well established that several components of the MAPK and PI3K pathways, as well as different proteins associated with these cascades, can be modified by S-nitrosylation. Overall, this review presents a better understanding of Ras S-nitrosylation, increasing the knowledge on the dynamics of these proteins in the presence of NO and the underlying implications in cellular signaling.
Keywords	S-nitrosylation ; cell proliferation ; chemical bonding ; cysteine ; guanosinetriphosphatase ; mitogen-activated protein kinase ; moieties ; nitric oxide ; oncogenes ; phosphatidylinositol 3-kinase ; signal transduction ; thiols
Language	English
Dates of publication	2023-0804
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox12081562
Database	NAL-Catalogue (AGRICOLA)

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Article: Sucrose Hydrolysis in a Continuous Packed-Bed Reactor with Auto-immobilise Aspergillus niger Biocatalyst Obtained by Solid-State Fermentation

Martínez-Ruiz, Antonio / Tovar-Castro, Luz / Aguilar, Cristóbal N. / Saucedo-Castañeda, Gerardo / Favela-Torres, Ernesto

Applied biochemistry and biotechnology. 2022 Mar., v. 194, no. 3

2022

Abstract: Invertase from Aspergillus niger C28B25 was produced by solid-state fermentation (SSF). Fermented solids were used directly as a biocatalyst for batch and continuous hydrolysis of sucrose in a packed-bed reactor under different operational conditions ... ...

Abstract	Invertase from Aspergillus niger C28B25 was produced by solid-state fermentation (SSF). Fermented solids were used directly as a biocatalyst for batch and continuous hydrolysis of sucrose in a packed-bed reactor under different operational conditions with various temperatures, sucrose concentrations, and feed flow rates. The SSF allowed obtaining a biocatalyst with an invertase activity of 82.2 U/g db. The biocatalyst maintained its activity in the range of 40 to 70 °C for at least 70 h of continuous operation. In a 20-mL packed bed reactor, the highest hydrolysis rate (12.3 g/g db h) was obtained at 40 °C with 2 M sucrose. Continuous hydrolysis in 20-mL and 200-mL reactors at 60 °C led to sucrose hydrolysis above 60% (8.5 residence times) and above 55% (4.5 residence times), respectively. The auto-immobilised biocatalyst produced by SSF without recovery, purification, and immobilisation stages offers an economical alternative for developing accessible biocatalysts that can be applied in batch or continuous sucrose hydrolysis processes. This study shows the potential of biocatalyst production by SSF for other enzymatic systems.
Keywords	Aspergillus niger ; beta-fructofuranosidase ; biocatalysts ; biotechnology ; hydrolysis ; solid state fermentation ; sucrose
Language	English
Dates of publication	2022-03
Size	p. 1327-1339.
Publishing place	Springer US
Document type	Article
ZDB-ID	392344-7
ISSN	0273-2289
ISSN	0273-2289
DOI	10.1007/s12010-021-03737-z
Database	NAL-Catalogue (AGRICOLA)

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Z 80/718: Show issues

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Article ; Online: Measurement of Superoxide Production in Acute Hypoxia by Fixed-Cell Microscopy.

Hernansanz-Agustín, Pablo / Choya-Foces, Carmen / Martínez-Ruiz, Antonio

Methods in molecular biology (Clifton, N.J.)

2020 Volume 2202, Page(s) 43–50

Abstract: Redox signaling implication in cell adaptation to hypoxia has been studied for a long time, both in long-term and acute responses. However, measurement of superoxide and other reactive oxygen species (ROS) in acute hypoxia is technically challenging, for ...

Abstract	Redox signaling implication in cell adaptation to hypoxia has been studied for a long time, both in long-term and acute responses. However, measurement of superoxide and other reactive oxygen species (ROS) in acute hypoxia is technically challenging, for example, because of the need to overcome the effect of cell reoxygenation before measurement.Here we describe a method we have developed for measuring superoxide production in acute hypoxia using the fluorescent probe dihydroethidine in fixed-cell microscopy. The method allows measuring the kinetics of superoxide production (or other ROS with the appropriate probes) by incubating the probe in different time windows during hypoxia incubation.
MeSH term(s)	Animals ; Cell Hypoxia/physiology ; Cell Line ; Dicarbethoxydihydrocollidine/analogs & derivatives ; Dicarbethoxydihydrocollidine/metabolism ; Humans ; Hypoxia/metabolism ; Microscopy/methods ; Microscopy, Fluorescence/methods ; Mitochondria/metabolism ; Oxidation-Reduction ; Oxygen/metabolism ; Reactive Oxygen Species/analysis ; Reactive Oxygen Species/metabolism ; Superoxides/analysis ; Superoxides/metabolism
Chemical Substances	Reactive Oxygen Species ; dihydroethidine ; Superoxides (11062-77-4) ; Dicarbethoxydihydrocollidine (632-93-9) ; Oxygen (S88TT14065)
Language	English
Publishing date	2020-08-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-0896-8_3
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Preparation and evaluation of (R)-(-)-carvone-doped polymeric resins as potential antibacterial dental materials.

Martínez-Ruiz, Erick Osvaldo / González-López, José Abraham / Ledezma-Pérez, Antonio Serguei / Espinosa-Neira, Roberto / Betancourt-Galindo, Rebeca / Neira-Velázquez, María Guadalupe / Treviño-Martínez, María Esther

Journal of the mechanical behavior of biomedical materials

2023 Volume 147, Page(s) 106108

Abstract: Objectives: This study aimed to develop and evaluate resin-based antibacterial materials incorporating carvone for restorative dentistry. The objectives included assessing antimicrobial activity, conversion degree, mechanical properties, hydrolytic and ... ...

Abstract	Objectives: This study aimed to develop and evaluate resin-based antibacterial materials incorporating carvone for restorative dentistry. The objectives included assessing antimicrobial activity, conversion degree, mechanical properties, hydrolytic and hygroscopic behavior, cytotoxicity, among others. Methodology: Carvone was incorporated into resin-based materials following established protocols. Antimicrobial activity was evaluated against S. Aureus. Conversion degree, polimerization kinetics, mechanical properties, hydrolytic and hygroscopic behavior, cytotoxicity, and other properties were assessed using standardized tests and methodologies. Results: Carvone-incorporated materials demonstrated significant antimicrobial activity, minimal changes in conversion degree, comparable mechanical properties, improved hydrolytic and hygroscopic behavior, and lack of cytotoxicity. Antimicrobial resins were obtained due to the hydrophobic nature of carvone and its ability to diffuse through the cell walls of microorganisms, causing membrane damage. The polymerization process yielded successful conversion, ensuring adequate material performance. Significance: This study showcases that incorporating carvone into methacrylate-based resins can confer antimicrobial properties while preserving key material attributes. Antimicrobial activity against S. aureus is achieved without cytotoxicity in human fibroblasts. While flexural properties are affected only at carvone concentrations exceeding 9%, conversion degree and polymerization kinetics remain stable, except for a specific experimental formulation. These findings highlight the balanced integration of carvone. However, further work, including assessing antimicrobial performance against specific strains like S. Mutans and/or C. Albicans, and evaluating long-term effectiveness, is essential to establish the potential of these materials for dental restorations.
MeSH term(s)	Humans ; Composite Resins/chemistry ; Staphylococcus aureus ; Materials Testing ; Methacrylates/chemistry ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry ; Polymers ; Dental Materials
Chemical Substances	Composite Resins ; carvone (75GK9XIA8I) ; Methacrylates ; Anti-Bacterial Agents ; Polymers ; Dental Materials
Language	English
Publishing date	2023-09-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2378381-3
ISSN	1878-0180 ; 1751-6161
ISSN (online)	1878-0180
ISSN	1751-6161
DOI	10.1016/j.jmbbm.2023.106108
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book: Aproximación a una bibliografía de Don Antonio Nariño y Álvarez

Ruiz Martínez, Eduardo

(Publicaciones del Instituto Caro y Cuervo : Serie bibliográfica ; 15)

1995

Author's details	Eduardo Ruiz Martínez
Series title	Publicaciones del Instituto Caro y Cuervo : Serie bibliográfica ; 15
Language	Spanish
Size	390 S
Publisher	Instituto Caro y Cuervo
Publishing place	Santafé de Bogotá
Document type	Book
Database	Former special subject collection: coastal and deep sea fishing

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