LIVIVO - Search results -

Search results

Result 1 - 10 of total 90503

Search options

Article: Traditional Chinese Medicine formula Dai-Zong-Fang alleviating hepatic steatosis in

Zhang, Li-Wei / Zhu, Li-Li / Zhu, Xiao-Yun / Fu, Shou-Qiang / Liu, Xi-Ming

2024 Volume 15, Page(s) 1337057

Abstract: Introduction: ...

Abstract	Introduction:
Language	English
Publishing date	2024-01-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2024.1337057
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article: The Chinese herbal medicine Dai-Zong-Fang promotes browning of white adipocytes

Xu, Jing / Zhang, Li-Wei / Feng, Hui / Tang, Yang / Fu, Shou-Qiang / Liu, Xi-Ming / Zhu, Xiao-Yun

Frontiers in pharmacology

2023 Volume 14, Page(s) 1176443

Abstract: Introduction: ...

Abstract	Introduction:
Language	English
Publishing date	2023-05-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2023.1176443
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Premature conclusions about the signal-to-noise ratio in structural equation modeling research: A commentary on Yuan and Fang (2023).

Schuberth, Florian / Schamberger, Tamara / Rönkkö, Mikko / Liu, Yide / Henseler, Jörg

The British journal of mathematical and statistical psychology

2023 Volume 76, Issue 3, Page(s) 682–694

Abstract: In a recent article published in this journal, Yuan and Fang (British Journal of Mathematical and ... thus corresponds to greater values of the [SNR]." In our commentary, we show that Yuan and Fang have made ... their methodological choice regarding CB-SEM and regression analysis with composites on the findings of Yuan and Fang ...

Abstract	In a recent article published in this journal, Yuan and Fang (British Journal of Mathematical and Statistical Psychology, 2023) suggest comparing structural equation modeling (SEM), also known as covariance-based SEM (CB-SEM), estimated by normal-distribution-based maximum likelihood (NML), to regression analysis with (weighted) composites estimated by least squares (LS) in terms of their signal-to-noise ratio (SNR). They summarize their findings in the statement that "[c]ontrary to the common belief that CB-SEM is the preferred method for the analysis of observational data, this article shows that regression analysis via weighted composites yields parameter estimates with much smaller standard errors, and thus corresponds to greater values of the [SNR]." In our commentary, we show that Yuan and Fang have made several incorrect assumptions and claims. Consequently, we recommend that empirical researchers not base their methodological choice regarding CB-SEM and regression analysis with composites on the findings of Yuan and Fang as these findings are premature and require further research.
MeSH term(s)	Latent Class Analysis ; Signal-To-Noise Ratio ; Least-Squares Analysis ; Research Design ; Normal Distribution
Language	English
Publishing date	2023-04-18
Publishing country	England
Document type	Observational Study ; Journal Article ; Comment
ZDB-ID	218109-5
ISSN	2044-8317 ; 0007-1102
ISSN (online)	2044-8317
ISSN	0007-1102
DOI	10.1111/bmsp.12304
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 981: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Jing-Fang powder ethyl acetate extracts attenuate atopic dermatitis by modulating T-cell activity.

Zhao, Ge / Tong, Yue / Xu, Jie / Zhu, Wenjing / Zeng, Jiuseng / Liu, Rong / Luan, Fei / Zeng, Nan

Molecular immunology

2023 Volume 160, Page(s) 133–149

Abstract: Jing-Fang powder ethyl acetate extract (JFEE) and its isolated C (JFEE-C) possess favorable ...

Abstract	Jing-Fang powder ethyl acetate extract (JFEE) and its isolated C (JFEE-C) possess favorable anti-inflammatory and anti-allergic properties; however, their inhibitory effects on T cell activity remain unknown. In vitro, Jurkat T cells and primary mouse CD4
MeSH term(s)	Animals ; Mice ; Dermatitis, Atopic/drug therapy ; Dermatitis, Atopic/chemically induced ; Interleukin-2 ; Powders/adverse effects ; Powders/metabolism ; NF-kappa B/metabolism ; Cytokines/metabolism ; CD4-Positive T-Lymphocytes/metabolism ; Mice, Inbred BALB C ; Plant Extracts/pharmacology
Chemical Substances	Interleukin-2 ; ethyl acetate (76845O8NMZ) ; Powders ; NF-kappa B ; Cytokines ; Plant Extracts
Language	English
Publishing date	2023-07-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	424427-8
ISSN	1872-9142 ; 0161-5890
ISSN (online)	1872-9142
ISSN	0161-5890
DOI	10.1016/j.molimm.2023.07.002
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 166: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Qingxin Kaiqiao Fang decreases Tau hyperphosphorylation in Alzheimer's disease via the PI3K/Akt/GSK3β pathway in vitro and in vivo.

Liu, Shuo / Xu, Luting / Shen, Yan / Wang, Liuying / Lai, Xiaoxiao / Hu, Haiyan

Journal of ethnopharmacology

2023 Volume 318, Issue Pt B, Page(s) 117031

Abstract: ... depression, eliminating turbidity, cultivating positivity, and dispelling evil spirits. Qingxin Kaiqiao Fang ...

Abstract	Ethnopharmacological relevance: Alzheimer's disease (AD) belongs to the category of "senile dementia" in traditional Chinese medicine. AD is associated with brain emptiness or collaterals blocked by phlegm-heat. "Fumanjian" from Jingyue Quanshu treats dementia by promoting qi circulation, alleviating depression, eliminating turbidity, cultivating positivity, and dispelling evil spirits. Qingxin Kaiqiao Fang (QKF), derived from Fumanjian, is effective in treating AD owing to previously mentioned clinical effects. Elucidating the mechanism(s) of action of QKF on AD associated with phlegm-heat may be beneficial for therapeutic management; however, further research is needed. Aim of the study: This study aimed to determine the role of the PI3K/Akt pathway in AD, especially the specific effector protein involved, and explore the efficacy of QKF in treating AD by modulating the PI3K/Akt signal. Materials and methods: High-performance liquid chromatography-Q-orbitrap-mass spectrometry was used to analyze the chemical components of QKF. Subsequently, APP/PS1 double-transgenic mice were used for behavioral tests, and hematoxylin-eosin and Nissl staining were used to assess the neuroprotective and cognitive effects of QKF. Cerebrospinal fluid pharmacology was used in in vitro validation, and Aβ Results: We identified 295 chemical components in the water extract of QKF.QKF improved spatial cognition and learning memory in APP/PS1 mice, protected PC12 cell morphology, improved cell survival, reduced Aβ Conclusions: QKF can improve spatial cognition, learning, and memory abilities in APP/PS1 mice and protect PC12 cells. Decreasing the Tau hyperphosphorylation in AD exhibits curative efficacy on AD via the PI3K/Akt/GSK3β pathway in vitro and in vivo.
MeSH term(s)	Rats ; Mice ; Animals ; Alzheimer Disease/drug therapy ; Alzheimer Disease/complications ; tau Proteins/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Glycogen Synthase Kinase 3 beta/metabolism ; Amyloid beta-Peptides/metabolism ; Phosphorylation ; Mice, Transgenic ; Maze Learning
Chemical Substances	tau Proteins ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Glycogen Synthase Kinase 3 beta (EC 2.7.11.1) ; Amyloid beta-Peptides
Language	English
Publishing date	2023-08-12
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.117031
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1494: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: San Jie Tong Mai Fang Protects Against Atherosclerosis Progression by Regulating Macroautophagy through the PI3K/AKT/mTOR Signaling Pathway.

Li, Pengfei / Li, Hongyu / Li, Xiaohui / Li, Shuangdi / Xu, Hanying / Cui, Junfeng / Cheng, Guangyu / Liu, Yinghui / Xu, Xiaolin / Xin, Yuning / Liu, Aidong

Journal of cardiovascular pharmacology

2023 Volume 82, Issue 4, Page(s) 333–343

Abstract: ... autophagy. We previously reported that the herbal formula San Jie Tong Mai Fang (SJTMF) elicits lipid ...

Abstract	Abstract: Many studies have confirmed that macrophage autophagy injury negatively impacts the pathogenesis of atherosclerosis (AS). Meanwhile, the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway affects AS progression by regulating macrophage autophagy. We previously reported that the herbal formula San Jie Tong Mai Fang (SJTMF) elicits lipid regulatory and anti-inflammatory properties. Hence, the current study used an ApoE -/- high-fat diet-fed mouse model to determine whether SJTMF elicits protective effects against AS progression by means of the regulation of macrophage autophagy through the PI3K/AKT/mTOR signaling pathway. Our results show that SJTMF reduced the number of atherosclerotic plaques, foam cell formation, and intimal thickness in mouse aorta. In addition, SJTMF improved blood lipid metabolism and inflammatory levels in mice. We also observed that SJTMF caused macrophages to be polarized toward the M2 phenotype through the inhibition of the PI3K/AKT/mTOR signaling pathway. In addition, the abundances of LC3-II/I and beclin1 proteins-key autophagy molecules-were increased, whereas that of p62 was decreased, resulting in the promotion of macrophage autophagy. Taken together, these findings indicate that SJTMF may regulate the polarization of macrophages by inhibiting the PI3K/AKT/mTOR signaling pathway, thereby reducing atherosclerotic plaque damage in ApoE -/- mice, thereby promoting macrophage autophagy and eliciting a significant antiarteriosclerosis effect. Hence, SJTMF may represent a promising new candidate drug for the treatment of AS.
MeSH term(s)	Mice ; Animals ; Proto-Oncogene Proteins c-akt/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Phosphatidylinositol 3-Kinase/metabolism ; Macroautophagy ; TOR Serine-Threonine Kinases/metabolism ; Mice, Knockout, ApoE ; Signal Transduction ; Atherosclerosis/drug therapy ; Atherosclerosis/prevention & control ; Atherosclerosis/genetics ; Plaque, Atherosclerotic ; Autophagy ; Apolipoproteins E/pharmacology ; Mammals/metabolism
Chemical Substances	Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Phosphatidylinositol 3-Kinase (EC 2.7.1.137) ; TOR Serine-Threonine Kinases (EC 2.7.11.1) ; Apolipoproteins E
Language	English
Publishing date	2023-10-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	391970-5
ISSN	1533-4023 ; 0160-2446
ISSN (online)	1533-4023
ISSN	0160-2446
DOI	10.1097/FJC.0000000000001452
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1471: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Zigui-Yichong-Fang protects against cyclophosphamide-induced premature ovarian insufficiency via the SIRT1/Foxo3a pathway.

Xiu, Zi / Tang, Siling / Kong, Peng / Yan, Mengxuan / Tong, Xue / Liu, Xueping / Liang, Xiao / Li, Rongxia / Duan, Yancang

Journal of ethnopharmacology

2023 Volume 314, Page(s) 116608

Abstract: Ethnopharmacological relevance: Zigui-Yichong-Fang (ZGYCF) is a traditional Chinese medicine ...

Abstract	Ethnopharmacological relevance: Zigui-Yichong-Fang (ZGYCF) is a traditional Chinese medicine prescription for the treatment of infertility and premature ovarian insufficiency (POI). It is clinically used to regulate hormone levels, improve ovarian reserve and increase pregnancy rate. However, the exact mechanism of action is not yet clear. Aims of the study: This study aimed to explore the potential impact and mechanism of ZGYCF on POI, and provide a scientific basis for its clinical application. Materials and methods: UHPLC‒MS/MS was used to identify the main compounds of ZGYCF. Female 8-week-old C57BL/6N mice were randomized into four group containing the vehicle control (Veh) group, the cyclophosphamide (CTX) model group, the low-dose ZGYCF (CTX-ZG-L) group and the high-dose ZGYCF (CTX-ZG-H) group. A mouse POI model was induced with a single intraperitoneal injection of CTX, and the therapeutic effects of different doses of ZGYCF on POI were evaluated according to the ovarian weight coefficient, serum AMH, serum E Results: A total of 37 compounds in ZGYCF were identified. ZGYCF attenuated the morphological changes in ovarian tissue in POI model mice, increased serum AMH and E Conclusions: Acetylated Foxo3a plays an important role in the occurrence of POI. ZGYCF improves the ovarian reserve of CTX-induced POI mice by activating SIRT1-mediated deacetylation of Foxo3a, and played a role in the treatment of POI. SIRT1 may be a novel target for ZGYCF to ameliorate POI.
MeSH term(s)	Humans ; Female ; Mice ; Animals ; Sirtuin 1/metabolism ; Phosphatidylinositol 3-Kinases ; Tandem Mass Spectrometry ; Mice, Inbred C57BL ; Primary Ovarian Insufficiency/chemically induced ; Primary Ovarian Insufficiency/drug therapy ; Primary Ovarian Insufficiency/prevention & control ; Cyclophosphamide/toxicity ; Menopause, Premature ; Estradiol/therapeutic use ; Disease Models, Animal
Chemical Substances	Sirtuin 1 (EC 3.5.1.-) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Cyclophosphamide (8N3DW7272P) ; Estradiol (4TI98Z838E) ; SIRT1 protein, human (EC 3.5.1.-) ; Sirt1 protein, mouse (EC 3.5.1.-)
Language	English
Publishing date	2023-05-05
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116608
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1494: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Study on Fu-Fang-Jin-Qian-Cao Inhibiting Autophagy in Calcium Oxalate-induced Renal Injury by UHPLC/Q-TOF-MS-based Metabonomics and Network Pharmacology Approaches.

Liu, Wen-Rui / Li, Mao-Ting / Zhou, Qi / Gao, Song-Yan / Hou, Jie-Bin / Yang, Guo-Bin / Liu, Nan-Mei / Jia-Yan / Yu, Jian-Peng / Cheng, Jin / Guo, Zhi-Yong

Combinatorial chemistry & high throughput screening

2024 Volume 27, Issue 1, Page(s) 90–100

Abstract: Introduction: Fu-Fang-Jin-Qian-Cao is a Chinese herbal preparation used to treat urinary calculi ... Fu-Fang-Jin-Qian-Cao can protect renal tubular epithelial cells from calcium oxalateinduced renal ... pharmacology to study the mechanism of Fu-Fang-Jin-Qian-Cao inhibiting autophagy in calcium oxalate-induced ...

Abstract	Introduction: Fu-Fang-Jin-Qian-Cao is a Chinese herbal preparation used to treat urinary calculi. Fu-Fang-Jin-Qian-Cao can protect renal tubular epithelial cells from calcium oxalateinduced renal injury by inhibiting ROS-mediated autopathy. The mechanism still needs further exploration. Metabonomics is a new subject; the combination of metabolomics and network pharmacology can find pathways for drugs to act on targets more efficiently. Methods: Comprehensive metabolomics and network pharmacology to study the mechanism of Fu-Fang-Jin-Qian-Cao inhibiting autophagy in calcium oxalate-induced renal injury. Based on UHPLC-Q-TOF-MS, combined with biochemical analysis, a mice model of Calcium oxalateinduced renal injury was established to study the therapeutic effect of Fu-Fang-Jin-Qian-Cao. Based on the network pharmacology, the target signaling pathway and the protective effect of Fu- Fang-Jin-Qian-Cao on Calcium oxalate-induced renal injury by inhibiting autophagy were explored. Autophagy-related proteins LC3-II, BECN1, ATG5, and ATG7 were studied by immunohistochemistry. Results: Combining network pharmacology and metabolomics, 50 differential metabolites and 2482 targets related to these metabolites were found. Subsequently, the targets enriched in PI3KAkt, MAPK and Ras signaling pathways. LC3-II, BECN1, ATG5 and ATG7 were up-regulated in Calcium oxalate-induced renal injury. All of them could be reversed after the Fu-Fang-Jin-Qian- Cao treatment. Conclusions: Fu-Fang-Jin-Qian-Cao can reverse ROS-induced activation of the MAPK signaling pathway and inhibition of the PI3K-Akt signaling pathway, thereby reducing autophagy damage of renal tubular epithelial cells in Calcium oxalate-induced renal injury.
MeSH term(s)	Mice ; Animals ; Calcium Oxalate/metabolism ; Calcium Oxalate/pharmacology ; Calcium/metabolism ; Chromatography, High Pressure Liquid ; Network Pharmacology ; Phosphatidylinositol 3-Kinases/metabolism ; Reactive Oxygen Species/metabolism ; Kidney/metabolism ; Autophagy ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/metabolism
Chemical Substances	Calcium Oxalate (2612HC57YE) ; Calcium (SY7Q814VUP) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Reactive Oxygen Species ; Drugs, Chinese Herbal
Language	English
Publishing date	2024-01-12
Publishing country	United Arab Emirates
Document type	Journal Article
ZDB-ID	2064785-2
ISSN	1875-5402 ; 1386-2073
ISSN (online)	1875-5402
ISSN	1386-2073
DOI	10.2174/1386207326666230515151302
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5577: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Evaluation of the effectiveness and mechanism of action of the Chang-Kang-Fang formula combined with bifid triple viable capsules on diarrhea-predominant irritable bowel syndrome.

Sun, Jing / Zhang, Mengqiu / Liu, Wei / Liu, Youqian / Zhang, Dongjian / Fan, Xinyu / Zhang, Jian / Li, Tian / Lu, Min

Frontiers in microbiology

2023 Volume 14, Page(s) 1160783

Abstract: Introduction: The Chang-Kang-Fang (CKF) formula, a traditional Chinese herbal formula ...

Abstract	Introduction: The Chang-Kang-Fang (CKF) formula, a traditional Chinese herbal formula, can decrease serotonin (5-HT) levels and treat irritable bowel syndrome (IBS). Probiotics have a better synergistic effect on diarrhea-predominant IBS (IBS-D) when combined with 5-HT Methods: The rat models of IBS-D were induced by gavage with senna decoction plus restraint stress. The CKF formula, PFK and their combination were administered to the rats. Their effects were evaluated based on general condition of the rats and the AWR score. The levels of 5-HT and fos protein in the colon and hippocampus were measured by immunohistochemistry. The levels of SP and VIP, as well as ZO-1 and occludin in the colon, were determined by enzyme-linked immunosorbent assay and immunohistochemistry. The intestinal microbiota in faeces was analyzed by 16S rRNA high-throughput sequencing. Results: The results showed that the oral CKF formula combined with PFK (CKF + PFK) could significantly relieve the symptoms of IBS-D, including elevating the weight rate and decreasing the AWR score. Compared with the MC group, administration of CKF + PFK significantly reduced the expression of fos in the colon and hippocampus and that of 5-HT, SP and VIP in the colon and increased the levels of 5-HT in the hippocampus and ZO-1 and occludin in the colon. The above indexes exhibited statistical significance in the CKF + PFK group relative to those in the other groups. Moreover, treatment with CKF + PFK improved the diversity of intestinal microbiota and the abundance of Conclusions: The CKF formula combined with PFK may have a synergistic effect on IBS-D by slowing gastrointestinal motility, lowering visceral hypersensitivity, enhancing the intestinal barrier function and modulating the composition of intestinal microbiota.
Language	English
Publishing date	2023-06-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2023.1160783
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Protective effects and mechanisms of Yi Qi Huo Xue Fang in cerebral ischemic stroke based on network pharmacology and experimental verification.

Li, Jiamin / Zhang, Tiantian / Liu, Kan / Hu, Guoheng

Journal of ethnopharmacology

2023 Volume 314, Page(s) 116611

Abstract: Ethnopharmacological relevance: Yi Qi Huo Xue Fang (YQHXF) is an effective formula for treating ...

Abstract	Ethnopharmacological relevance: Yi Qi Huo Xue Fang (YQHXF) is an effective formula for treating cerebral ischemic stroke (CIS). However, its active ingredients and mechanism of action remain unclear. Aim of the study: This study aimed to reveal the mechanism of action of YQHXF in the treatment of ischemic stroke based on network pharmacology and experimental validation. Materials and methods: This study identified the chemical components in YQHXF and the components absorbed by rat serum based on UPLC-Q-TOF/MS technology and used network pharmacology to predict key candidate targets. A protein-protein-interaction (P-P-I) network was constructed using String 11.0 database and Cytoscape, and R software for gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis. Finally, molecular docking combined with animal experiments was used to verify network pharmacology results. Results: This study identified and confirmed 36 chemical components of YQHXF and five chemical ingredients that were absorbed into the blood of rats and screened 66 key candidate targets. All targets in the P-P-I network were mainly related to inflammation and vascular processes. KEGG enrichment results revealed that these 66 key candidate targets were primarily involved in the "AGE-RAGE signaling pathway," "TNF-α signaling pathway, and "T cell receptor signaling pathway." Molecular docking results revealed that Prostaglandin-endoperoxidase synthase 2(PTGS-2), Nitric oxide synthase, endothelial (NOS3), and peroxisome proliferator-activated receptor gamma (PPARG) were more stably bound to their active ingredients. Animal experiments demonstrated that YQHXF promoted M2 polarization, inhibited M1 polarization in microglia, and promoted angiogenesis, which may be related to the PPARG pathway. Conclusion: This study revealed the key active components and effective targets of YQHXF, identified the mechanism of action of YQHXF, laid the foundation for further research on YQHXF, and provided ideas for developing new drugs for CIS.
MeSH term(s)	Animals ; Rats ; Ischemic Stroke ; Molecular Docking Simulation ; Network Pharmacology ; PPAR gamma ; Stroke/drug therapy ; Biological Products ; Cyclooxygenase 2 ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use
Chemical Substances	PPAR gamma ; Biological Products ; Cyclooxygenase 2 (EC 1.14.99.1) ; Drugs, Chinese Herbal
Language	English
Publishing date	2023-05-10
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116611
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1494: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito