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Article ; Online: Bringing to Light the Importance of the miRNA Methylome in Colorectal Cancer Prognosis Through Electrochemical Bioplatforms.

Povedano, Eloy / Ruiz-Valdepeñas Montiel, Víctor / Sebuyoya, Ravery / Torrente-Rodríguez, Rebeca M / Garranzo-Asensio, Maria / Montero-Calle, Ana / Pingarrón, José M / Barderas, Rodrigo / Bartosik, Martin / Campuzano, Susana

Analytical chemistry

2024 Volume 96, Issue 11, Page(s) 4580–4588

Abstract: This work reports the first electrochemical bioplatforms developed for the determination of the total contents of either target miRNA or methylated target miRNA. The bioplatforms are based on the hybridization of the target miRNA with a synthetic ... ...

Abstract	This work reports the first electrochemical bioplatforms developed for the determination of the total contents of either target miRNA or methylated target miRNA. The bioplatforms are based on the hybridization of the target miRNA with a synthetic biotinylated DNA probe, the capture of the formed DNA/miRNA heterohybrids on the surface of magnetic microcarriers, and their recognition with an antibody selective to these heterohybrids or to the
MeSH term(s)	Humans ; MicroRNAs/genetics ; MicroRNAs/analysis ; Epigenome ; Nucleic Acid Hybridization/methods ; Antibodies/genetics ; Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/genetics ; Prognosis ; Biosensing Techniques/methods
Chemical Substances	MicroRNAs ; Antibodies
Language	English
Publishing date	2024-02-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c05474
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Dextran-coated nanoparticles as immunosensing platforms: Consideration of polyaldehyde density, nanoparticle size and functionality.

Gao, Shipeng / Torrente-Rodríguez, Rebeca M / Pedrero, María / Pingarrón, José M / Campuzano, Susana / Rocha-Martin, Javier / Guisán, José M

Talanta

2022 Volume 247, Page(s) 123549

Abstract: Magnetic nanoparticles (MNPs) can be used as antibody carriers in a wide range of immunosensing applications. The conjugation chemistry for preparing antibody-MNP bionanohybrids should assure the nanoparticle's colloidal dispersity, directional ... ...

Abstract	Magnetic nanoparticles (MNPs) can be used as antibody carriers in a wide range of immunosensing applications. The conjugation chemistry for preparing antibody-MNP bionanohybrids should assure the nanoparticle's colloidal dispersity, directional conformation and high biofunctionality retention of attached antibodies. In this work, peroxidase (HRP) was selected as model target analyte, and stable antibody-MNP conjugates were prepared using polyaldehyde-dextrans as multivalent linkers, also to prevent nanoparticles agglomeration and steric shielding of non-specific proteins. Under the manipulation of the oxidation variables, MNP-conjugated antibody showed the highest Fab accessibility, of 1.32 μmol analyte per μmol antibody, corresponding to 139 μmol aldehyde per gram of nanocarrier (5 mM NaIO
MeSH term(s)	Antibodies ; Biosensing Techniques/methods ; Dextrans/chemistry ; Immunoassay/methods ; Magnetics ; Magnetite Nanoparticles/chemistry ; Nanoparticles/chemistry
Chemical Substances	Antibodies ; Dextrans ; Magnetite Nanoparticles
Language	English
Publishing date	2022-05-16
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2022.123549
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Disposable electrochemical immunoplatform to shed light on the role of the multifunctional glycoprotein TIM-1 in cancer cells invasion.

Quinchia, Jennifer / Blázquez-García, Marina / Torrente-Rodríguez, Rebeca M / Ruiz-Valdepeñas Montiel, Víctor / Serafín, Verónica / Rejas-González, Raquel / Montero-Calle, Ana / Orozco, Jahir / Pingarrón, José M / Barderas, Rodrigo / Campuzano, Susana

Talanta

2023 Volume 267, Page(s) 125155

Abstract: Detecting overexpression of cancer biomarkers is an excellent tool for diagnostic/prognostic and follow-up of patients with cancer or their response to treatment. This work illustrates the relevance of interrogating the levels of T-cell immunoglobulin ... ...

Abstract	Detecting overexpression of cancer biomarkers is an excellent tool for diagnostic/prognostic and follow-up of patients with cancer or their response to treatment. This work illustrates the relevance of interrogating the levels of T-cell immunoglobulin and mucin domain 1 (TIM-1) protein as a diagnostic/prognostic biomarker of high-prevalence breast and lung cancers by using an amperometric disposable magnetic microparticles-assisted immunoplatform. The developed method integrates the inherent advantages of carboxylic acid-functionalized magnetic beads (HOOC-MBs) as pre-concentrator support and the amperometric transduction at screen-printed carbon electrodes (SPCEs). The immunoplatform involves a sandwich-type immunoassay assembled on HOOC-MBs through the specific capture/labeling of TIM-1 using capture antibodies and horseradish peroxidase (HRP)-conjugated biotinylated detection antibodies as biorecognition elements. The magnetic immunoconjugates were confined onto the working electrode (WE) surface of the SPCEs for amperometric detection using the hydroquinone/hydrogen peroxide/HRP (HQ/H
MeSH term(s)	Humans ; Hydrogen Peroxide/chemistry ; Antibodies/chemistry ; Immunoconjugates/chemistry ; Magnetics ; Horseradish Peroxidase ; Glycoproteins ; Biosensing Techniques/methods ; Electrochemical Techniques/methods ; Electrodes ; Immunoassay/methods ; Limit of Detection ; Neoplasms
Chemical Substances	Hydrogen Peroxide (BBX060AN9V) ; Antibodies ; Immunoconjugates ; Horseradish Peroxidase (EC 1.11.1.-) ; Glycoproteins
Language	English
Publishing date	2023-09-04
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2023.125155
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Article: p53 and p63 Proteoforms Derived from Alternative Splicing Possess Differential Seroreactivity in Colorectal Cancer with Distinct Diagnostic Ability from the Canonical Proteins.

Montero-Calle, Ana / Garranzo-Asensio, María / Torrente-Rodríguez, Rebeca M / Ruiz-Valdepeñas Montiel, Víctor / Poves, Carmen / Dziaková, Jana / Sanz, Rodrigo / Díaz Del Arco, Cristina / Pingarrón, José Manuel / Fernández-Aceñero, María Jesús / Campuzano, Susana / Barderas, Rodrigo

Cancers

2023 Volume 15, Issue 7

Abstract: Colorectal cancer (CRC) is the third most common cancer and the second most frequent cause of cancer-related death worldwide. The detection in plasma samples of autoantibodies against specific tumor-associated antigens has been demonstrated to be useful ... ...

Abstract	Colorectal cancer (CRC) is the third most common cancer and the second most frequent cause of cancer-related death worldwide. The detection in plasma samples of autoantibodies against specific tumor-associated antigens has been demonstrated to be useful for the early diagnosis of CRC by liquid biopsy. However, new studies related to the humoral immune response in cancer are needed to enable blood-based diagnosis of the disease. Here, our aim was to characterize the humoral immune response associated with the different p53 and p63 proteoforms derived from alternative splicing and previously described as aberrantly expressed in CRC. Thus, here we investigated the diagnostic ability of the twelve p53 proteoforms and the eight p63 proteoforms described to date, and their specific N-terminal and C-terminal end peptides, by means of luminescence HaloTag beads immunoassays. Full-length proteoforms or specific peptides were cloned as HaloTag fusion proteins and their seroreactivity analyzed using plasma from CRC patients at stages I-IV (
Language	English
Publishing date	2023-03-31
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers15072102
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Article ; Online: SARS-CoV-2 RapidPlex: A Graphene-Based Multiplexed Telemedicine Platform for Rapid and Low-Cost COVID-19 Diagnosis and Monitoring.

Torrente-Rodríguez, Rebeca M / Lukas, Heather / Tu, Jiaobing / Min, Jihong / Yang, Yiran / Xu, Changhao / Rossiter, Harry B / Gao, Wei

Matter

2020 Volume 3, Issue 6, Page(s) 1981–1998

Abstract: The COVID-19 pandemic is an ongoing global challenge for public health systems. Ultrasensitive and early identification of infection is critical in preventing widespread COVID-19 infection by presymptomatic and asymptomatic individuals, especially in the ...

Abstract	The COVID-19 pandemic is an ongoing global challenge for public health systems. Ultrasensitive and early identification of infection is critical in preventing widespread COVID-19 infection by presymptomatic and asymptomatic individuals, especially in the community and in-home settings. We demonstrate a multiplexed, portable, wireless electrochemical platform for ultra-rapid detection of COVID-19: the SARS-CoV-2 RapidPlex. It detects viral antigen nucleocapsid protein, IgM and IgG antibodies, as well as the inflammatory biomarker C-reactive protein, based on our mass-producible laser-engraved graphene electrodes. We demonstrate ultrasensitive, highly selective, and rapid electrochemical detection in the physiologically relevant ranges. We successfully evaluated the applicability of our SARS-CoV-2 RapidPlex platform with COVID-19-positive and COVID-19-negative blood and saliva samples. Based on this pilot study, our multiplexed immunosensor platform may allow for high-frequency at-home testing for COVID-19 telemedicine diagnosis and monitoring.
Keywords	covid19
Language	English
Publishing date	2020-10-05
Publishing country	United States
Document type	Journal Article
ISSN	2590-2385
ISSN (online)	2590-2385
DOI	10.1016/j.matt.2020.09.027
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Article: Dextran-coated nanoparticles as immunosensing platforms: Consideration of polyaldehyde density, nanoparticle size and functionality

Gao, Shipeng / Torrente-Rodríguez, Rebeca M. / Pedrero, María / Pingarrón, José M. / Campuzano, Susana / Rocha-Martin, Javier / Guisán, José M.

Talanta. 2022 Sept. 01, v. 247

2022

Abstract	Magnetic nanoparticles (MNPs) can be used as antibody carriers in a wide range of immunosensing applications. The conjugation chemistry for preparing antibody-MNP bionanohybrids should assure the nanoparticle's colloidal dispersity, directional conformation and high biofunctionality retention of attached antibodies. In this work, peroxidase (HRP) was selected as model target analyte, and stable antibody-MNP conjugates were prepared using polyaldehyde-dextrans as multivalent linkers, also to prevent nanoparticles agglomeration and steric shielding of non-specific proteins. Under the manipulation of the oxidation variables, MNP-conjugated antibody showed the highest Fab accessibility, of 1.32 μmol analyte per μmol antibody, corresponding to 139 μmol aldehyde per gram of nanocarrier (5 mM NaIO₄, 4 h). Demonstrating anti-interference advantage up to 10% serum, colorimetric immunoassay gave a detection limit (LOD) of 300 ng mL⁻¹, while electrochemical transduction led to a considerable (680 times) improvement, with a LOD of 0.44 ng mL⁻¹. In addition, polyaldehyde-dextran showed priority over polycarboxylated-dextran as the multivalent antibody crosslinker for MNPs in terms of sensitivity and LOD value, while immunosensors constructed with carboxylated magnetic microbeads (HOOC-MBs) outperformed MNPs-based immunoplatforms. This work sheds light on the importance of surface chemistry (type and density of functional groups) and the dimension (nanosize vs micrometer) of magnetic carriers to conjugate antibodies with better directional orientation and improve the analytical performance of the resulting immunosensors.
Keywords	aldehydes ; antibodies ; blood serum ; carboxylation ; colorimetry ; detection limit ; electrochemistry ; immunoassays ; immunosensors ; magnetism ; microbeads ; nanocarriers ; oxidation ; peroxidase
Language	English
Dates of publication	2022-0901
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2022.123549
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Article: Towards Control and Oversight of SARS-CoV-2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors.

Torrente-Rodríguez, Rebeca M / Montero-Calle, Ana / San Bartolomé, Clara / Cano, Olga / Vázquez, Mónica / Iglesias-Caballero, María / Corral-Lugo, Andrés / McConnell, Michael J / Pascal, Mariona / Mas, Vicente / Pingarrón, José M / Barderas, Rodrigo / Campuzano, Susana

Angewandte Chemie (Weinheim an der Bergstrasse, Germany)

2022 Volume 134, Issue 28, Page(s) e202203662

Abstract: The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house ... ...

Abstract	The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N- and S-specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP-conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen-printed electrodes using the HQ/HRP/H
Language	English
Publishing date	2022-05-24
Publishing country	Germany
Document type	Journal Article
ZDB-ID	506609-8
ISSN	1521-3757 ; 0044-8249 ; 0932-2140
ISSN (online)	1521-3757
ISSN	0044-8249 ; 0932-2140
DOI	10.1002/ange.202203662
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Article ; Online: Towards Control and Oversight of SARS-CoV-2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors.

Angewandte Chemie (International ed. in English)

2022 Volume 61, Issue 28, Page(s) e202203662

Abstract	The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N- and S-specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP-conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen-printed electrodes using the HQ/HRP/H
MeSH term(s)	Antibodies, Viral ; Biosensing Techniques ; COVID-19/diagnosis ; COVID-19 Testing ; Humans ; Immunity ; Immunoglobulin G ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus
Chemical Substances	Antibodies, Viral ; Immunoglobulin G ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
Language	English
Publishing date	2022-05-24
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2011836-3
ISSN	1521-3773 ; 1433-7851
ISSN (online)	1521-3773
ISSN	1433-7851
DOI	10.1002/anie.202203662
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Article ; Online: Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases.

Torrente-Rodríguez, Rebeca M / Martín, Cristina Muñoz-San / Gamella, Maria / Pedrero, María / Martínez-Bosch, Neus / Navarro, Pilar / García de Frutos, Pablo / Pingarrón, José M / Campuzano, Susana

Biosensors

2021 Volume 11, Issue 6

Abstract: A magnetic beads (MB)-involved amperometric immunosensor for the determination of ST2, a member of the IL1 receptor family, is reported in this work. The method utilizes a sandwich immunoassay and disposable screen-printed carbon electrodes (SPCEs). ... ...

Abstract	A magnetic beads (MB)-involved amperometric immunosensor for the determination of ST2, a member of the IL1 receptor family, is reported in this work. The method utilizes a sandwich immunoassay and disposable screen-printed carbon electrodes (SPCEs). Magnetic immunoconjugates built on the surface of carboxylic acid-microsized magnetic particles (HOOC-MBs) were used to selectively capture ST2. A biotinylated secondary antibody further conjugated with a streptavidin peroxidase conjugate (Strep-HRP) was used to accomplish the sandwiching of the target protein. The immune platform exhibits great selectivity and a low limit of detection (39.6 pg mL
MeSH term(s)	Antibodies ; Biosensing Techniques ; Carbon ; Electrochemical Techniques ; Electrodes ; Enzyme-Linked Immunosorbent Assay ; Humans ; Hydrogen Peroxide ; Immunoassay ; Limit of Detection ; Magnetics ; Neoplasms/diagnosis
Chemical Substances	Antibodies ; Carbon (7440-44-0) ; Hydrogen Peroxide (BBX060AN9V)
Language	English
Publishing date	2021-06-21
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662125-3
ISSN	2079-6374 ; 2079-6374
ISSN (online)	2079-6374
ISSN	2079-6374
DOI	10.3390/bios11060202
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Article ; Online: Magnetic microbeads-based amperometric immunoplatform for the rapid and sensitive detection of N6-methyladenosine to assist in metastatic cancer cells discrimination.

Povedano, Eloy / Gamella, María / Torrente-Rodríguez, Rebeca M / Montero-Calle, Ana / Pedrero, María / Solís-Fernández, Guillermo / Navarro-Villoslada, Fernando / Barderas, Rodrigo / Campuzano, Susana / Pingarrón, José M

Biosensors & bioelectronics

2020 Volume 171, Page(s) 112708

Abstract: This work describes the preparation of an immunoplatform for the sensitive and selective determination of N6-methyladenosine (m6A). The simple and fast protocol involves for the first time the use of micromagnetic immunoconjugates to establish a direct ... ...

Abstract	This work describes the preparation of an immunoplatform for the sensitive and selective determination of N6-methyladenosine (m6A). The simple and fast protocol involves for the first time the use of micromagnetic immunoconjugates to establish a direct competitive assay between the m6A target and a biotinylated RNA oligomer bearing a single m6A enzymatically labelled with a commercial conjugate of streptavidin-peroxidase (Strep-HRP) as tracer. The cathodic current change measured in the presence of H
MeSH term(s)	Adenosine/analogs & derivatives ; Biosensing Techniques ; Hydrogen Peroxide ; Limit of Detection ; Magnetic Phenomena ; Microspheres ; Neoplasms
Chemical Substances	Hydrogen Peroxide (BBX060AN9V) ; N-methyladenosine (CLE6G00625) ; Adenosine (K72T3FS567)
Language	English
Publishing date	2020-10-08
Publishing country	England
Document type	Journal Article
ZDB-ID	1011023-9
ISSN	1873-4235 ; 0956-5663
ISSN (online)	1873-4235
ISSN	0956-5663
DOI	10.1016/j.bios.2020.112708
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