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Book ; Online: Biological Activities of Ribosome-Inactivating Proteins

Ferreras, José Miguel / Citores, Lucía

2023

Keywords	Research & information: general ; Biology, life sciences ; ribosome inactivating protein ; therapeutic applications ; immunotoxin ; anti-HIV ; anti-cancer ; antiviral proteins ; ribosome inactivating proteins ; Phytolacca dioica L. ; protein purification ; tobacco necrosis virus ; Adenia ; apoptosis ; kirkiin ; lectins ; neuroblastoma ; ribosome-inactivating proteins ; ricin ; toxic enzymes ; adenine polynucleotide glycosylase ; antiviral therapy ; human virus ; ribosome-inactivating protein (RIP) ; rRNA glycosylase (EC 3.2.2.22) ; virus-resistant transgenic plant (VRTP) ; clathrin ; dynamin ; ebulin ; endocytosis ; intracellular transport ; lectin ; rRNA N-glycosylase ; ribosome-inactivating protein ; plant toxin ; type I RIP ; rRNA glycosylase activity (EC 3.2.2.22) ; protein isolation ; protein sequencing ; mass spectrometry ; antifungal activity ; agretti ; cytotoxicity ; edible plants ; rRNA N-glycosylases ; anticancer agents ; galactose ; nanoLC-tandem mass spectrometry (nLC-MS/MS) ; protein synthesis (inhibition) ; sugar binding ; abrin ; intranasal ; lungs ; alveolar epithelial type II cells ; neutrophils ; alveolar-capillary barrier ; junction proteins ; glycocalyx ; Curcin ; Curcin C ; osteosarcoma U20S cells ; n/a
Language	English
Size	1 electronic resource (196 pages)
Publisher	MDPI - Multidisciplinary Digital Publishing Institute
Publishing place	Basel
Document type	Book ; Online
Note	English
HBZ-ID	HT030722295
ISBN	9783036568232 ; 3036568239
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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Article ; Online: c-MET/VEGFR-2 co-localisation impacts on survival following bevacizumab therapy in epithelial ovarian cancer: an exploratory biomarker study of the phase 3 ICON7 trial.

Morgan, Robert D / Ferreras, Cristina / Peset, Isabel / Avizienyte, Egle / Renehan, Andrew G / Edmondson, Richard J / Murphy, Alexander D / Nicum, Shibani / Van Brussel, Thomas / Clamp, Andrew R / Lambrechts, Diether / Zhou, Cong / Jayson, Gordon C

BMC medicine

2022 Volume 20, Issue 1, Page(s) 59

Abstract: ... ovarian cancer (EOC). Pre-clinical data showed that the c-MET/VEGFR-2 heterocomplex negates VEGF inhibition ... through activation of c-MET signalling, leading to a more invasive and metastatic phenotype. We evaluated ... the clinical significance of c-MET and VEGFR-2 co-localisation and its association with VEGF pathway-related ...

Abstract	Introduction: Bevacizumab improves survival outcomes in women diagnosed with epithelial ovarian cancer (EOC). Pre-clinical data showed that the c-MET/VEGFR-2 heterocomplex negates VEGF inhibition through activation of c-MET signalling, leading to a more invasive and metastatic phenotype. We evaluated the clinical significance of c-MET and VEGFR-2 co-localisation and its association with VEGF pathway-related single nucleotide polymorphisms (SNPs) in women participating in the phase 3 trial, ICON7 (ClinicalTrials.gov identifier: NCT00262847). Materials and methods: Patients had FIGO stage I-IIA grade 3/poorly differentiated or clear cell carcinoma or stage IIB-IV epithelial ovarian, primary peritoneal or fallopian tube cancer. Immunofluorescence staining for co-localised c-MET and VEGFR-2 on tissue microarrays and genotyping of germline DNA from peripheral blood leukocytes for VEGFA and VEGFR-2 SNPs was performed. The significance of these biomarkers was assessed against survival. Results: Tissue microarrays from 178 women underwent immunofluorescence staining. Multivariable analysis showed that greater c-MET/VEGFR-2 co-localisation predicted worse OS in patients treated with bevacizumab after adjusting for FIGO stage and debulking surgery outcome (hazard ratio [HR] 1.034, 95% confidence interval [95%CI] 1.010-1.059). Women in the c-MET/VEGFR-2 Conclusions: In bevacizumab-treated women diagnosed with EOC, high c-MET/VEGFR-2 co-localisation on tumour tissue and the VEGFR-2 rs2305945 G/G variant, which may be biologically related, were associated with worse survival outcomes.
MeSH term(s)	Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Bevacizumab/therapeutic use ; Biomarkers ; Carcinoma, Ovarian Epithelial/drug therapy ; Carcinoma, Ovarian Epithelial/genetics ; Female ; Humans ; Ovarian Neoplasms/drug therapy ; Ovarian Neoplasms/genetics ; Ovarian Neoplasms/pathology ; Vascular Endothelial Growth Factor Receptor-2/genetics ; Vascular Endothelial Growth Factor Receptor-2/therapeutic use
Chemical Substances	Biomarkers ; Bevacizumab (2S9ZZM9Q9V) ; Vascular Endothelial Growth Factor Receptor-2 (EC 2.7.10.1)
Language	English
Publishing date	2022-02-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2131669-7
ISSN	1741-7015 ; 1741-7015
ISSN (online)	1741-7015
ISSN	1741-7015
DOI	10.1186/s12916-022-02270-y
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Article ; Online: c-MET/VEGFR-2 co-localisation impacts on survival following bevacizumab therapy in epithelial ovarian cancer

Robert D. Morgan / Cristina Ferreras / Isabel Peset / Egle Avizienyte / Andrew G. Renehan / Richard J. Edmondson / Alexander D. Murphy / Shibani Nicum / Thomas Van Brussel / Andrew R. Clamp / Diether Lambrechts / Cong Zhou / Gordon C. Jayson

BMC Medicine, Vol 20, Iss 1, Pp 1-

an exploratory biomarker study of the phase 3 ICON7 trial

2022 Volume 11

Abstract: ... underwent quantitative immunofluorescence for c-MET/VEGFR-2 co-expression and genetic sequencing ... for single nucleotide polymorphisms (SNPs) VEGF-pathway genes. High c-MET/VEGFR-2 co-localisation on tumour tissue independently ...

Abstract	Highlights Tissue microarrays and germline DNA from women recruited to the phase 3 trial, ICON7, underwent quantitative immunofluorescence for c-MET/VEGFR-2 co-expression and genetic sequencing for single nucleotide polymorphisms (SNPs) VEGF-pathway genes. High c-MET/VEGFR-2 co-localisation on tumour tissue independently predicted worse survival in bevacizumab-treated epithelial ovarian cancer. The VEGFR-2 rs2305945 SNPs also independently predict worse survival in bevacizumab-treated epithelial ovarian cancer.
Keywords	Epithelial ovarian cancer ; Bevacizumab ; c-MET ; VEGFR-2 ; Single-nucleotide polymorphisms ; Medicine ; R
Language	English
Publishing date	2022-02-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Experimental infection of lambs with C and S-type strains of Mycobacterium avium subspecies paratuberculosis: immunological and pathological findings.

Fernández, Miguel / Benavides, Julio / Sevilla, Iker A / Fuertes, Miguel / Castaño, Pablo / Delgado, Laetitia / García Marín, J Francisco / Garrido, Joseba M / Ferreras, M Carmen / Pérez, Valentín

Veterinary research

2014 Volume 45, Page(s) 5

Abstract: ... are cattle (C) and sheep (S) strains. An experimental infection was conducted to establish the effect ... two of S- (22G and the pigmented Ovicap49) and one of C- (764) type, and the reference K-10 strain (C ... Specific antibody and IFN-γ production was significantly higher in animals infected with the C strains, while no ...

Abstract	The two main genotypes of recognized isolates of Mycobacterium avium subsp. paratuberculosis (Map) are cattle (C) and sheep (S) strains. An experimental infection was conducted to establish the effect of Map strain on the pathogenesis of ovine paratuberculosis. Twenty-four out of thirty 1.5-month-old Assaf lambs were divided into 4 groups of 6 and infected orally with three low passage field isolates, two of S- (22G and the pigmented Ovicap49) and one of C- (764) type, and the reference K-10 strain (C type). The remaining six animals were unchallenged controls. Animals were euthanized at 150 and 390 days post-infection (dpi). Throughout the experiment, the peripheral immune response was assessed and histological and molecular (PCR) studies were conducted on samples of intestine and related lymphoid tissue. Specific antibody and IFN-γ production was significantly higher in animals infected with the C strains, while no consistent IFN- γ responses were observed in the S-type strain infected groups. A positive intradermal skin test response was detected in all infected groups. Lambs infected with S-type strains had granulomatous lesions restricted to the lymphoid tissue with no differences in the lesion intensity over time. In both C-type strain groups, lesions were more severe at 150 dpi while at 390 dpi lesions, characterized by well-demarcated granulomas with fibrosis, decreased in severity. Only infected lambs were positive to PCR. These results suggest that the strain of Map has a strong influence over the immune and pathological responses developed by the host. Lesions induced by C-type strains in lambs show a regressive character and tend to decrease as the infection progresses.
MeSH term(s)	Animals ; Enzyme-Linked Immunosorbent Assay/veterinary ; Immunity, Cellular ; Interferon-gamma/blood ; Interferon-gamma Release Tests/veterinary ; Intestines/immunology ; Intradermal Tests/veterinary ; Lymphoid Tissue/immunology ; Mycobacterium avium subsp. paratuberculosis/genetics ; Mycobacterium avium subsp. paratuberculosis/physiology ; Paratuberculosis/immunology ; Paratuberculosis/pathology ; Paratuberculosis/virology ; Polymerase Chain Reaction/veterinary ; Random Allocation ; Sheep ; Sheep Diseases/immunology ; Sheep Diseases/pathology ; Sheep Diseases/virology
Chemical Substances	Interferon-gamma (82115-62-6)
Language	English
Publishing date	2014-01-16
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1146298-x
ISSN	1297-9716 ; 0928-4249
ISSN (online)	1297-9716
ISSN	0928-4249
DOI	10.1186/1297-9716-45-5
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Article: Heterogenized Gold(I), Gold(III), and Palladium(II) Complexes for C-C Bond Reactions

Corma, Avelino / González-Arellano, Camino / Iglesias, Marta / Pérez-Ferreras, Susana / Sánchez, Félix

Synlett

2007 Volume 2007, Issue 11, Page(s) 1771–1774

Abstract: Recycling of heterogenized gold(I), gold(III), and palladium(II) complexes could be achieved for Suzuki and Sonogashira cross-coupling reaction between iodo benzene and arylboronic acids or alkynes. Au(I) and Pd(II) afford selectively nonsymmetrical ... ...

Abstract	Recycling of heterogenized gold(I), gold(III), and palladium(II) complexes could be achieved for Suzuki and Sonogashira cross-coupling reaction between iodo benzene and arylboronic acids or alkynes. Au(I) and Pd(II) afford selectively nonsymmetrical biaryl compounds, while gold(III) complexes can only catalyze the arylboronic or alkynes homocoupling. Recycling occurs without loss of the catalytic activity after several reaction cycles.
Keywords	C-C coupling ; gold ; Suzuki ; Sonogashira ; homogeneous catalysis ; heterogenized
Language	English
Publishing date	2007-06-25
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	2042012-2
ISSN	1437-2096 ; 0936-5214
ISSN (online)	1437-2096
ISSN	0936-5214
DOI	10.1055/s-2007-984500
Database	Thieme publisher's database

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Article ; Online: Antifungal Activity of Ribosome-Inactivating Proteins.

Iglesias, Rosario / Citores, Lucía / Gay, Claudia C / Ferreras, José M

Toxins

2024 Volume 16, Issue 4

Abstract: The control of crop diseases caused by fungi remains a major problem and there is a need to find effective fungicides that are environmentally friendly. Plants are an excellent source for this purpose because they have developed defense mechanisms to ... ...

Abstract	The control of crop diseases caused by fungi remains a major problem and there is a need to find effective fungicides that are environmentally friendly. Plants are an excellent source for this purpose because they have developed defense mechanisms to cope with fungal infections. Among the plant proteins that play a role in defense are ribosome-inactivating proteins (RIPs), enzymes obtained mainly from angiosperms that, in addition to inactivating ribosomes, have been studied as antiviral, fungicidal, and insecticidal proteins. In this review, we summarize and discuss the potential use of RIPs (and other proteins with similar activity) as antifungal agents, with special emphasis on RIP/fungus specificity, possible mechanisms of antifungal action, and the use of RIP genes to obtain fungus-resistant transgenic plants. It also highlights the fact that these proteins also have antiviral and insecticidal activity, which makes them very versatile tools for crop protection.
MeSH term(s)	Ribosome Inactivating Proteins/pharmacology ; Antifungal Agents/pharmacology ; Plant Proteins/pharmacology ; Plant Proteins/genetics ; Fungi/drug effects ; Plant Diseases/microbiology ; Plant Diseases/prevention & control ; Plants, Genetically Modified ; Animals ; Fungicides, Industrial/pharmacology
Chemical Substances	Ribosome Inactivating Proteins (EC 3.2.2.22) ; Antifungal Agents ; Plant Proteins ; Fungicides, Industrial
Language	English
Publishing date	2024-04-15
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2518395-3
ISSN	2072-6651 ; 2072-6651
ISSN (online)	2072-6651
ISSN	2072-6651
DOI	10.3390/toxins16040192
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Mycobacterium bovis in Egyptian mongoose, Spain.

Ferreras-Colino, Elisa / Descalzo, Esther / Romero, Beatriz / Gortázar, Christian / Ferreras, Pablo

Zoonoses and public health

2023 Volume 70, Issue 4, Page(s) 365–368

Abstract: Tuberculosis caused by Mycobacterium bovis and other related mycobacteria has been reported in a wide range of mammals worldwide. In the case of the Herpestidae family, Mycobacterium mungi and M. bovis, both belonging to the Mycobacterium tuberculosis ... ...

Abstract	Tuberculosis caused by Mycobacterium bovis and other related mycobacteria has been reported in a wide range of mammals worldwide. In the case of the Herpestidae family, Mycobacterium mungi and M. bovis, both belonging to the Mycobacterium tuberculosis Complex, have been reported in banded mongooses (Mungos mungo) in Africa and in Egyptian mongooses (Herpestes ichneumon) in Portugal, respectively. Thus, we hypothesized that Tuberculosis may occur in Egyptian mongooses from Spain. Twenty-five found dead Egyptian mongooses were necropsied in order to detect macroscopic TB-compatible lesions and mandibular lymph nodes and lungs were cultured onto mycobacteria-specific growth media. We isolated M. bovis in 3/25 Egyptian mongooses (12.00%, IC95: 4.17-29.96%) and identified spoligotypes SB0121 (2/3) and SB0134 (1). No macroscopic TB-compatible lesions were observed. To the best of our knowledge, this is the first report of M. bovis in Egyptian mongoose in Spain, as well as the only study that includes spolygotyping in this species. Although the absence of visible lesions suggests a minor role of the Egyptian mongoose in Tuberculosis epidemiology, further research thereon is encouraged.
MeSH term(s)	Animals ; Mycobacterium bovis ; Herpestidae/microbiology ; Spain/epidemiology ; Tuberculosis/epidemiology ; Tuberculosis/veterinary ; Portugal
Language	English
Publishing date	2023-02-28
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2271118-1
ISSN	1863-2378 ; 1863-1959
ISSN (online)	1863-2378
ISSN	1863-1959
DOI	10.1111/zph.13033
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Amplificación de la región 5´UTR-C del genoma de los cuatro serotipos de Virus Dengue

Francisco Triana-Alonso / Guillermo Comach / Héctor Graterol / Ana Celia Ferreras / Juana Ledia Triana-Alonso / Elizabeth Ferrer / Daria Elena Camacho

Salus Online, Vol 16, Iss 3, Pp 61-

2012 Volume 69

Abstract: ... of such processes by antiviral molecules. The objective of this work was to amplify the 5'UTR-C (Untranslated Region ... According to the specified conditions, products were obtained from the 5'UTR-C with RT-PCR robust and ...

Abstract	Dengue virus infections (DENV) are the most important viral disease in terms of morbidity and mortality. The DENV genome is a single-stranded RNA with two untranslated regions (UTR) at the ends (5' UTR and 3' UTR) flanking an open reading frame (ORF). The 5 UTR and 3' UTR are important in the mechanisms of viral replication and protein synthesis, making them potential targets for regulation or inhibition of such processes by antiviral molecules. The objective of this work was to amplify the 5'UTR-C (Untranslated Region-Capsid) of the genome of the four DENV serotypes after optimizing the Reverse Transcription coupled to Polymerase Chain Reaction (RT-PCR), which could be used to assess viral translation processes in eukaryotic systems in vitro and its inhibition by potential antiviral molecules. Strains of different serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) were used and assays were performed with different concentrations of the primers and Taq polymerase and reverse transcriptase enzymes. The results indicated that the best response was obtained with primer concentrations of 0.5 µmol/L (DENV-1 and DENV-3), 1 µmol/L (DENV-2) and 0.75 µmol/L (DENV-4). The enzymes used showed high efficiency at the lowest quantity tested (1.25 U). According to the specified conditions, products were obtained from the 5'UTR-C with RT-PCR robust and reliable for amplification of these products
Keywords	dengue virus ; dengue ; RT-PCR ; 5`UTR ; Medicine (General) ; R5-920 ; Medicine ; R ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Subject code	572
Language	Spanish
Publishing date	2012-12-01T00:00:00Z
Publisher	Facultad de Ciencias de la Salud, Universidad de Carabobo
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Experimental infection of lambs with C and S-type strains of Mycobacterium avium subspecies paratuberculosis: immunological and pathological findings

Fernández, Miguel / Benavides, Julio / Sevilla, Iker A / Fuertes, Miguel / Castaño, Pablo / Delgado, Laetitia / Marín, J Francisco García / Garrido, Joseba M / Ferreras, M Carmen / Pérez, Valentín

Veterinary research. 2014 Dec., v. 45, no. 1

2014

Abstract: ... are cattle (C) and sheep (S) strains. An experimental infection was conducted to establish the effect ... two of S- (22G and the pigmented Ovicap49) and one of C– (764) type, and the reference K-10 strain (C ... Specific antibody and IFN-γ production was significantly higher in animals infected with the C strains, while no ...

Abstract	The two main genotypes of recognized isolates of Mycobacterium avium subsp. paratuberculosis (Map) are cattle (C) and sheep (S) strains. An experimental infection was conducted to establish the effect of Map strain on the pathogenesis of ovine paratuberculosis. Twenty-four out of thirty 1.5-month-old Assaf lambs were divided into 4 groups of 6 and infected orally with three low passage field isolates, two of S- (22G and the pigmented Ovicap49) and one of C– (764) type, and the reference K-10 strain (C type). The remaining six animals were unchallenged controls. Animals were euthanized at 150 and 390 days post-infection (dpi). Throughout the experiment, the peripheral immune response was assessed and histological and molecular (PCR) studies were conducted on samples of intestine and related lymphoid tissue. Specific antibody and IFN-γ production was significantly higher in animals infected with the C strains, while no consistent IFN- γ responses were observed in the S-type strain infected groups. A positive intradermal skin test response was detected in all infected groups. Lambs infected with S-type strains had granulomatous lesions restricted to the lymphoid tissue with no differences in the lesion intensity over time. In both C–type strain groups, lesions were more severe at 150 dpi while at 390 dpi lesions, characterized by well-demarcated granulomas with fibrosis, decreased in severity. Only infected lambs were positive to PCR. These results suggest that the strain of Map has a strong influence over the immune and pathological responses developed by the host. Lesions induced by C–type strains in lambs show a regressive character and tend to decrease as the infection progresses.
Keywords	Mycobacterium avium subsp. paratuberculosis ; cattle ; fibrosis ; genotype ; immune response ; interferon-gamma ; lambs ; paratuberculosis ; pathogenesis ; polymerase chain reaction
Language	English
Dates of publication	2014-12
Size	p. 327.
Publishing place	Springer-Verlag
Document type	Article
ZDB-ID	1146298-x
ISSN	1297-9716 ; 0928-4249
ISSN (online)	1297-9716
ISSN	0928-4249
DOI	10.1186/1297-9716-45-5
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Congenital dislocation of the knee with spontaneous resolution-A case report.

Vicente-Ferreira, Margarida / Ferreras, Cristina / Gomes, Ana Cristina / Freitas, Joana Manuel

Pediatrics international : official journal of the Japan Pediatric Society

2023 Volume 65, Issue 1, Page(s) e15710

Language	English
Publishing date	2023-12-18
Publishing country	Australia
Document type	Case Reports
ZDB-ID	1470376-2
ISSN	1442-200X ; 1328-8067
ISSN (online)	1442-200X
ISSN	1328-8067
DOI	10.1111/ped.15710
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