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Article ; Online: Sensitivity of Xylella fastidiosa subsp. pauca Salento-1 to light at 410 nm.

Bleve, Gianluca / Trivellin, Nicola / Chirizzi, Daniela / Tarantini, Annamaria / Orlandi, Viviana Teresa / Milano, Francesco

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

2024 Volume 23, Issue 4, Page(s) 793–801

Abstract: All over the world, from America to the Mediterranean Sea, the plant pathogen Xylella fastidiosa represents one of the most difficult challenges with many implications at ecological, agricultural, and economic levels. X. fastidiosa is a rod-shaped Gram- ... ...

Abstract	All over the world, from America to the Mediterranean Sea, the plant pathogen Xylella fastidiosa represents one of the most difficult challenges with many implications at ecological, agricultural, and economic levels. X. fastidiosa is a rod-shaped Gram-negative bacterium belonging to the family of Xanthomonadaceae. It grows at very low rates and infects a wide range of plants thanks to different vectors. Insects, through their stylets, suck a sap rich in nutrients and inject bacteria into xylem vessels. Since, until now, no antimicrobial treatment has been successfully applied to kill X. fastidiosa and/or prevent its diffusion, in this study, antimicrobial blue light (aBL) was explored as a potential anti-Xylella tool. Xylella fastidiosa subsp. pauca Salento-1, chosen as a model strain, showed a certain degree of sensitivity to light at 410 nm. The killing effect was light dose dependent and bacterial concentration dependent. These preliminary results support the potential of blue light in decontamination of agricultural equipment and/or plant surface; however, further investigations are needed for in vivo applications.
MeSH term(s)	Plant Diseases/prevention & control ; Plant Diseases/microbiology ; Xylella
Language	English
Publishing date	2024-04-05
Publishing country	England
Document type	Journal Article
ZDB-ID	2072584-X
ISSN	1474-9092 ; 1474-905X
ISSN (online)	1474-9092
ISSN	1474-905X
DOI	10.1007/s43630-024-00556-z
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Article ; Online: Detection of Anti-

Manca, Raffaella / Ciccarese, Giuseppina / Scaltrito, Domenico / Chirizzi, Daniela

Veterinary sciences

2022 Volume 9, Issue 2

Abstract: Neosporosis is recognized as one of the major causes of bovine abortion worldwide. Canids are the main definitive host for this parasite and the presence of dogs in the farm is an important factor for ... ...

Abstract	Neosporosis is recognized as one of the major causes of bovine abortion worldwide. Canids are the main definitive host for this parasite and the presence of dogs in the farm is an important factor for the
Language	English
Publishing date	2022-02-17
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2768971-2
ISSN	2306-7381 ; 2306-7381
ISSN (online)	2306-7381
ISSN	2306-7381
DOI	10.3390/vetsci9020087
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Article ; Online: Chemical characterization of red cells from the black sea urchin

Pagliara, Patrizia / Chirizzi, Daniela / Guascito, Maria Rachele

RSC advances

2021 Volume 11, Issue 43, Page(s) 27074–27083

Abstract: Red spherula cells (RSC) from sea urchin coelomic fluid have attracted great interest for their specific and intriguing properties, such as for example antimicrobial activities and immune response, that probably tie in with their red characteristic ... ...

Abstract	Red spherula cells (RSC) from sea urchin coelomic fluid have attracted great interest for their specific and intriguing properties, such as for example antimicrobial activities and immune response, that probably tie in with their red characteristic pigments. Although to date different studies have been reported aimed to chemically characterize their pigments extracted from the cells, few data are available about the chemical characterization of the cell surface. In this work, a systematic chemical characterization of the RSC surface by X-ray photoelectron spectroscopy (XPS) analysis is described. The results were compared with data on colorless cells from the same coelomic fluid sample. Our observations evidenced that the two cell types were characterized by the presence of different chemical functional groups. In particular, the colorless cells are dominated by the presence of alkyl, alcohol, amide, and carboxyl groups in accordance with other similar cell types, enriched in Na
Language	English
Publishing date	2021-08-09
Publishing country	England
Document type	Journal Article
ISSN	2046-2069
ISSN (online)	2046-2069
DOI	10.1039/d1ra03156b
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Article: SARS-CoV-2 concentrations and virus-laden aerosol size distributions in outdoor air in north and south of Italy

Chirizzi, D / Conte, M / Feltracco, M / Dinoi, A / Gregoris, E / Barbaro, E / La Bella, G / Ciccarese, G / La Salandra, G / Gambaro, A / Contini, D

Environment international. 2021 Jan., v. 146

2021

Abstract: ... diameter D < 0.056 µm) up to coarse particles (D > 18 µm). Air samples tested negative for the presence ...

Abstract	The COVID-19 disease spread at different rates in the different countries and in different regions of the same country, as happened in Italy. Transmission by contact or at close range due to large respiratory droplets is widely accepted, however, the role of airborne transmission due to small respiratory droplets emitted by infected individuals (also asymptomatic) is controversial. It was suggested that outdoor airborne transmission could play a role in determining the differences observed in the spread rate. Concentrations of virus-laden aerosol are still poorly known and contrasting results are reported, especially for outdoor environments. Here we investigated outdoor concentrations and size distributions of virus-laden aerosol simultaneously collected during the pandemic, in May 2020, in northern (Veneto) and southern (Apulia) regions of Italy. The two regions exhibited significantly different prevalence of COVID-19. Genetic material of SARS-CoV-2 (RNA) was determined, using both real time RT-PCR and ddPCR, in air samples collected using PM₁₀ samplers and cascade impactors able to separate 12 size ranges from nanoparticles (diameter D < 0.056 µm) up to coarse particles (D > 18 µm). Air samples tested negative for the presence of SARS-CoV-2 at both sites, viral particles concentrations were <0.8 copies m⁻³ in PM₁₀ and <0.4 copies m⁻³ in each size range investigated. Outdoor air in residential and urban areas was generally not infectious and safe for the public in both northern and southern Italy, with the possible exclusion of very crowded sites. Therefore, it is likely that outdoor airborne transmission does not explain the difference in the spread of COVID-19 observed in the two Italian regions.
Keywords	COVID-19 infection ; RNA ; Severe acute respiratory syndrome coronavirus 2 ; aerosols ; air ; airborne transmission ; environment ; pandemic ; Italy
Language	English
Dates of publication	2021-01
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	554791-x
ISSN	1873-6750 ; 0160-4120
ISSN (online)	1873-6750
ISSN	0160-4120
DOI	10.1016/j.envint.2020.106255
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Article ; Online: A novel nonenzymatic amperometric hydrogen peroxide sensor based on CuO@Cu2O nanowires embedded into poly(vinyl alcohol).

Chirizzi, Daniela / Guascito, Maria Rachele / Filippo, Emanuela / Tepore, Antonio

Talanta

2016 Volume 147, Page(s) 124–131

Abstract: A new, very simple, rapid and inexpensive nonenzymatic amperometric sensor for hydrogen peroxide (H2O2) detection is proposed. It is based on the immobilization of cupric/cuprous oxide core shell nanowires (CuO@Cu2O-NWs) in a poly(vinyl alcohol) (PVA) ... ...

Abstract	A new, very simple, rapid and inexpensive nonenzymatic amperometric sensor for hydrogen peroxide (H2O2) detection is proposed. It is based on the immobilization of cupric/cuprous oxide core shell nanowires (CuO@Cu2O-NWs) in a poly(vinyl alcohol) (PVA) matrix directly drop casted on a glassy carbon electrode surface to make a CuO@Cu2O core shell like NWs PVA embedded (CuO@Cu2O-NWs/PVA) sensor. CuO nanowires with mean diameters of 120-170nm and length in the range 2-5μm were grown by a simple catalyst-free thermal oxidation process based on resistive heating of pure copper wires at ambient conditions. The oxidation process of the copper wire surface led to the formation of a three layered structure: a thick Cu2O bottom layer, a CuO thin intermediate layer and CuO nanowires. CuO nanowires were carefully scratched from Cu2O layer with a sharp knife, dispersed into ethanol and sonicated. Then, the NWs were embedded in PVA matrix. The morphological and spectroscopic characterization of synthesized CuO-NWs and CuO@Cu2O-NWs/PVA were performed by transmission electron microscopy (TEM), selected area diffraction pattern (SAD), scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) analysis. Moreover a complete electrochemical characterization of these new CuO@Cu2O-NWs/PVA modified glassy carbon electrodes was performed by Cyclic Voltammetry (CV) and Cronoamperometry (CA) in phosphate buffer (pH=7; I=0.2) to investigate the sensing properties of this material against H2O2. The electrochemical performances of proposed sensors as high sensitivity, fast response, reproducibility and selectivity make them suitable for the quantitative determination of hydrogen peroxide substrate in batch analysis.
MeSH term(s)	Biosensing Techniques/instrumentation ; Chemistry Techniques, Analytical/instrumentation ; Chemistry Techniques, Analytical/methods ; Copper/chemistry ; Hydrogen Peroxide/analysis ; Hydrogen Peroxide/chemistry ; Microscopy, Electron, Transmission ; Nanowires/chemistry ; Polyvinyl Alcohol/chemistry ; Reproducibility of Results
Chemical Substances	Copper (789U1901C5) ; Polyvinyl Alcohol (9002-89-5) ; Hydrogen Peroxide (BBX060AN9V) ; cuprous oxide (T8BEA5064F) ; cupric oxide (V1XJQ704R4)
Language	English
Publishing date	2016-01-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2015.09.038
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Article: Potentiometric urea biosensor based on urease immobilized by an electrosynthesized poly(o-phenylenediamine) film with buffering capability

Chirizzi, Daniela / Malitesta, Cosimino

Sensors & Actuators: B. Chemical. 2011 Sept. 20, v. 157, no. 1

2011

Abstract: A simple and novel potentiometric biosensor for urea detection was prepared by employing an electrosynthesized polymer with buffering capability. It was obtained by deposition of a weighed amount of urease (Ur) at a glassy carbon (GC) electrode followed ... ...

Abstract	A simple and novel potentiometric biosensor for urea detection was prepared by employing an electrosynthesized polymer with buffering capability. It was obtained by deposition of a weighed amount of urease (Ur) at a glassy carbon (GC) electrode followed by immobilization by an electrosynthesized poly-o-phenylenediamine (PPD) film. An unconventional “upside-down” (UD) geometry was employed for the electrochemical cell. The response of GC/Ur/PPD sensor is linear with urea concentration in the range 10μM to 1mM (15mV/mM, R²=0.9999) due to buffering capability of PPD film, which represents a novel role of electrosynthesized polymers in their application to biosensors. At higher concentrations, the more common Nernstian response (28mV/decade, R²=0.9987) is observed. The sensor exhibits a sufficient sensitivity for practical determinations, rapid response and long term stability.
Keywords	biosensors ; carbon ; electrochemistry ; electrodes ; polymers ; urea ; urease
Language	English
Dates of publication	2011-0920
Size	p. 211-215.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	1021505-0
ISSN	0925-4005
ISSN	0925-4005
DOI	10.1016/j.snb.2011.03.051
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Article ; Online: Airborne concentrations of SARS-CoV-2 in indoor community environments in Italy.

Conte, Marianna / Feltracco, Matteo / Chirizzi, Daniela / Trabucco, Sara / Dinoi, Adelaide / Gregoris, Elena / Barbaro, Elena / La Bella, Gianfranco / Ciccarese, Giuseppina / Belosi, Franco / La Salandra, Giovanna / Gambaro, Andrea / Contini, Daniele

Environmental science and pollution research international

2021 Volume 29, Issue 10, Page(s) 13905–13916

Abstract: COVID-19 pandemic raised a debate regarding the role of airborne transmission. Information regarding virus-laden aerosol concentrations is still scarce in community indoors and what are the risks for general public and the efficiency of restriction ... ...

Abstract	COVID-19 pandemic raised a debate regarding the role of airborne transmission. Information regarding virus-laden aerosol concentrations is still scarce in community indoors and what are the risks for general public and the efficiency of restriction policies. This work investigates, for the first time in Italy, the presence of SARS-CoV-2 RNA in air samples collected in different community indoors (one train station, two food markets, one canteen, one shopping centre, one hair salon, and one pharmacy) in three Italian cities: metropolitan city of Venice (NE of Italy), Bologna (central Italy), and Lecce (SE of Italy). Air samples were collected during the maximum spread of the second wave of pandemic in Italy (November and December 2020). All collected samples tested negative for the presence of SARS-CoV-2, using both real-time RT-PCR and ddPCR, and no significant differences were observed comparing samples taken with and without customers. Modelling average concentrations, using influx of customers' data and local epidemiological information, indicated low values (i.e. < 0.8 copies m
MeSH term(s)	Air Microbiology ; COVID-19 ; Humans ; Italy ; Pandemics ; RNA, Viral ; SARS-CoV-2/isolation & purification
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-10-01
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1178791-0
ISSN	1614-7499 ; 0944-1344
ISSN (online)	1614-7499
ISSN	0944-1344
DOI	10.1007/s11356-021-16737-7
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Article ; Online: A review on measurements of SARS-CoV-2 genetic material in air in outdoor and indoor environments: Implication for airborne transmission.

Dinoi, Adelaide / Feltracco, Matteo / Chirizzi, Daniela / Trabucco, Sara / Conte, Marianna / Gregoris, Elena / Barbaro, Elena / La Bella, Gianfranco / Ciccarese, Giuseppina / Belosi, Franco / La Salandra, Giovanna / Gambaro, Andrea / Contini, Daniele

The Science of the total environment

2021 Volume 809, Page(s) 151137

Abstract: Airborne transmission of SARS-CoV-2 has been object of debate in the scientific community since the beginning of COVID-19 pandemic. This mechanism of transmission could arise from virus-laden aerosol released by infected individuals and it is influenced ... ...

Abstract	Airborne transmission of SARS-CoV-2 has been object of debate in the scientific community since the beginning of COVID-19 pandemic. This mechanism of transmission could arise from virus-laden aerosol released by infected individuals and it is influenced by several factors. Among these, the concentration and size distribution of virus-laden particles play an important role. The knowledge regarding aerosol transmission increases as new evidence is collected in different studies, even if it is not yet available a standard protocol regarding air sampling and analysis, which can create difficulties in the interpretation and application of results. This work reports a systematic review of current knowledge gained by 73 published papers on experimental determination of SARS-CoV-2 RNA in air comparing different environments: outdoors, indoor hospitals and healthcare settings, and public community indoors. Selected papers furnished 77 datasets: outdoor studies (9/77, 11.7%) and indoor studies (68/77. 88.3%). The indoor datasets in hospitals were the vast majority (58/68, 85.3%), and the remaining (10/68, 14.7%) were classified as community indoors. The fraction of studies having positive samples, as well as positivity rates (i.e. ratios between positive and total samples) are significantly larger in hospitals compared to the other typologies of sites. Contamination of surfaces was more frequent (in indoor datasets) compared to contamination of air samples; however, the average positivity rate was lower compared to that of air. Concentrations of SARS-CoV-2 RNA in air were highly variables and, on average, lower in outdoors compared to indoors. Among indoors, concentrations in community indoors appear to be lower than those in hospitals and healthcare settings.
MeSH term(s)	Aerosols ; Air Pollution, Indoor ; COVID-19 ; Humans ; Pandemics ; RNA, Viral ; SARS-CoV-2
Chemical Substances	Aerosols ; RNA, Viral
Language	English
Publishing date	2021-10-23
Publishing country	Netherlands
Document type	Journal Article ; Review ; Systematic Review
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2021.151137
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Article ; Online: Multispectral MRI with Dual Fluorinated Probes to Track Mononuclear Cell Activity in Mice.

Chirizzi, Cristina / De Battista, Davide / Tirotta, Ilaria / Metrangolo, Pierangelo / Comi, Giancarlo / Bombelli, Francesca Baldelli / Chaabane, Linda

Radiology

2019 Volume 291, Issue 2, Page(s) 351–357

Abstract: Background MRI with fluorine 19 ( ...

Abstract	Background MRI with fluorine 19 (
MeSH term(s)	Animals ; Cell Tracking/methods ; Fluorescent Dyes/pharmacokinetics ; Fluorescent Dyes/therapeutic use ; Fluorine-19 Magnetic Resonance Imaging/methods ; Leukocytes, Mononuclear/chemistry ; Leukocytes, Mononuclear/cytology ; Male ; Mice ; Mice, Inbred C57BL ; Nanoparticles/therapeutic use
Chemical Substances	Fluorescent Dyes
Language	English
Publishing date	2019-03-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	80324-8
ISSN	1527-1315 ; 0033-8419
ISSN (online)	1527-1315
ISSN	0033-8419
DOI	10.1148/radiol.2019181073
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Article ; Online: Characterization of hierarchical α-MoO

Filippo, Emanuela / Baldassarre, Francesca / Tepore, Marco / Guascito, Maria Rachele / Chirizzi, Daniela / Tepore, Antonio

Nanotechnology

2017 Volume 28, Issue 21, Page(s) 215601

Abstract: The growth of ... ...

Abstract	The growth of MoO
Language	English
Publishing date	2017-05-26
Publishing country	England
Document type	Journal Article
ZDB-ID	1362365-5
ISSN	1361-6528 ; 0957-4484
ISSN (online)	1361-6528
ISSN	0957-4484
DOI	10.1088/1361-6528/aa67c9
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