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  1. Article: Rapid evolution of SARS-CoV-2 in domestic cats.

    Bashor, Laura / Gagne, Roderick B / Bosco-Lauth, Angela / Stenglein, Mark / VandeWoude, Sue

    Virus evolution

    2022  Volume 8, Issue 2, Page(s) veac092

    Abstract: SARS-CoV-2 (SARS2) infection of a novel permissive host species can result in rapid viral evolution. Data suggest that felids are highly susceptible to SARS2 infection, and species-specific adaptation following human-to-felid transmission may occur. We ... ...

    Abstract SARS-CoV-2 (SARS2) infection of a novel permissive host species can result in rapid viral evolution. Data suggest that felids are highly susceptible to SARS2 infection, and species-specific adaptation following human-to-felid transmission may occur. We employed experimental infection and analysis of publicly available SARS2 sequences to observe variant emergence and selection in domestic cats. Three cohorts of cats (
    Language English
    Publishing date 2022-09-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 2818949-8
    ISSN 2057-1577
    ISSN 2057-1577
    DOI 10.1093/ve/veac092
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Selection of reference genes for quantitative PCR: identifying reference genes for airway epithelial cells exposed to

    Hampton, Thomas H / Koeppen, Katja / Bashor, Laura / Stanton, Bruce A

    American journal of physiology. Lung cellular and molecular physiology

    2020  Volume 319, Issue 2, Page(s) L256–L265

    Abstract: Most quantitative PCR (qPCR) experiments report differential expression relative to the expression of one or more reference genes. Therefore, when experimental conditions alter reference gene expression, qPCR results may be compromised. Little is known ... ...

    Abstract Most quantitative PCR (qPCR) experiments report differential expression relative to the expression of one or more reference genes. Therefore, when experimental conditions alter reference gene expression, qPCR results may be compromised. Little is known about the magnitude of this problem in practice. We found that reference gene responses are common and hard to predict and that their stability should be demonstrated in each experiment. Our reanalysis of 15 airway epithelia microarray data sets retrieved from the National Center for Biotechnology Information (NCBI) identified no common reference gene that was reliable in all 15 studies. Reanalysis of published RNA sequencing (RNA-seq) data in which human bronchial epithelial cells (HBEC) were exposed to
    MeSH term(s) Epithelial Cells/metabolism ; Epithelial Cells/microbiology ; Gene Expression/genetics ; Gene Expression Profiling/methods ; Humans ; Pseudomonas aeruginosa/pathogenicity ; RNA/genetics ; Real-Time Polymerase Chain Reaction ; Reproducibility of Results ; Respiratory Mucosa/metabolism ; Respiratory Mucosa/microbiology ; Sequence Analysis, RNA/methods
    Chemical Substances RNA (63231-63-0)
    Language English
    Publishing date 2020-06-10
    Publishing country United States
    Document type Journal Article ; Meta-Analysis ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1013184-x
    ISSN 1522-1504 ; 1040-0605
    ISSN (online) 1522-1504
    ISSN 1040-0605
    DOI 10.1152/ajplung.00158.2020
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  3. Article ; Online: Reply to Chandra: Severe Acute Respiratory Syndrome-Coronavirus-2 variant analysis reveals within-host selection of intra-host single nucleotide variants.

    Bashor, Laura / Gagne, Roderick B / Bosco-Lauth, Angela / Bowen, Richard A / Stenglein, Mark / VandeWoude, Sue

    Proceedings of the National Academy of Sciences of the United States of America

    2022  Volume 119, Issue 34, Page(s) e2206919119

    Language English
    Publishing date 2022-08-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2206919119
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  4. Article: The Density of the Lyme Disease Vector,

    Allen, David / Borgmann-Winter, Benjamin / Bashor, Laura / Ward, Jeremy

    Northeastern naturalist

    2019  Volume 26, Issue 3, Page(s) 545–560

    Abstract: Lyme disease is an emerging infectious disease of public health concern in the northeastern United States. The disease's vector, ...

    Abstract Lyme disease is an emerging infectious disease of public health concern in the northeastern United States. The disease's vector,
    Language English
    Publishing date 2019-07-24
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2117206-7
    ISSN 1938-5307 ; 1092-6194
    ISSN (online) 1938-5307
    ISSN 1092-6194
    DOI 10.1656/045.026.0307
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection.

    Bashor, Laura / Gagne, Roderick B / Bosco-Lauth, Angela / Bowen, Richard / Stenglein, Mark / VandeWoude, Sue

    bioRxiv : the preprint server for biology

    2021  

    Abstract: SARS-CoV-2 spillback from humans into domestic and wild animals has been well-documented. We compared variants of cell culture-expanded SARS-CoV-2 inoculum and virus recovered from four species following experimental exposure. Five nonsynonymous changes ... ...

    Abstract SARS-CoV-2 spillback from humans into domestic and wild animals has been well-documented. We compared variants of cell culture-expanded SARS-CoV-2 inoculum and virus recovered from four species following experimental exposure. Five nonsynonymous changes in nsp12, S, N and M genes were near fixation in the inoculum, but reverted to wild-type sequences in RNA recovered from dogs, cats and hamsters within 1-3 days post-exposure. Fourteen emergent variants were detected in viruses recovered from animals, including substitutions at spike positions H69, N501, and D614, which also vary in human lineages of concern. The rapidity of in vitro and in vivo SARS-CoV-2 selection reveals residues with functional significance during host-switching, illustrating the potential for spillback reservoir hosts to accelerate evolution, and demonstrating plasticity of viral adaptation in animal models.
    One-sentence summary: SARS-CoV-2 variants rapidly arise in non-human hosts, revealing viral evolution and potential risk for human reinfection.
    Language English
    Publishing date 2021-03-09
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2021.03.05.434135
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection.

    Bashor, Laura / Gagne, Roderick B / Bosco-Lauth, Angela M / Bowen, Richard A / Stenglein, Mark / VandeWoude, Sue

    Proceedings of the National Academy of Sciences of the United States of America

    2021  Volume 118, Issue 44

    Abstract: SARS-CoV-2 spillback from humans into domestic and wild animals has been well documented, and an accumulating number of studies illustrate that human-to-animal transmission is widespread in cats, mink, deer, and other species. Experimental inoculations ... ...

    Abstract SARS-CoV-2 spillback from humans into domestic and wild animals has been well documented, and an accumulating number of studies illustrate that human-to-animal transmission is widespread in cats, mink, deer, and other species. Experimental inoculations of cats, mink, and ferrets have perpetuated transmission cycles. We sequenced full genomes of Vero cell-expanded SARS-CoV-2 inoculum and viruses recovered from cats (
    MeSH term(s) Animals ; COVID-19/veterinary ; COVID-19/virology ; Cats ; Chlorocebus aethiops ; Dogs ; Evolution, Molecular ; Ferrets ; Gene Frequency ; Pets/virology ; SARS-CoV-2/genetics ; SARS-CoV-2/pathogenicity ; Selection, Genetic ; Vero Cells ; Viral Proteins/genetics
    Chemical Substances Viral Proteins
    Language English
    Publishing date 2021-10-29
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2105253118
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  7. Article ; Online: Potential effects of blood meal host on bacterial community composition in Ixodes scapularis nymphs.

    Landesman, William J / Mulder, Kenneth / Allan, Brian F / Bashor, Laura A / Keesing, Felicia / LoGiudice, Kathleen / Ostfeld, Richard S

    Ticks and tick-borne diseases

    2019  Volume 10, Issue 3, Page(s) 523–527

    Abstract: Tick microbiomes may play an important role in pathogen transmission. However, the drivers of microbiome variation are poorly understood, and this limitation has impeded mechanistic understanding of the functions of microbial communities for pathogen ... ...

    Abstract Tick microbiomes may play an important role in pathogen transmission. However, the drivers of microbiome variation are poorly understood, and this limitation has impeded mechanistic understanding of the functions of microbial communities for pathogen acquisition. The goal of this research was to characterize the role of the blood meal host in structuring the microbiome of Ixodes scapularis, the primary vector of Lyme disease in the eastern United States, and to determine if ticks that fed from different host species harbor distinct bacterial communities. We performed high-throughput 16S rDNA amplicon sequencing on I. scapularis nymphs that fed as larvae from known wildlife hosts: raccoon, Virginia opossum, striped skunk, red squirrel or gray squirrel. Using Analysis of Similarity, we found significant differences in the abundance-weighted Unifrac distance matrix among ticks fed from different host species (p =  0.048) and a highly significant difference in the weighted and unweighted Unifrac matrices for individuals within species (p <  0.01). This finding of associations between the blood meal host and I. scapularis microbiome demonstrates that the blood meal host may be a driver of microbiome variation that should be accounted for in studies of pathogen acquisition by ticks.
    MeSH term(s) Animals ; Animals, Wild/blood ; Animals, Wild/microbiology ; Bacteria/classification ; Bacteria/isolation & purification ; Blood ; Ixodes/microbiology ; Microbiota ; Nymph/microbiology ; RNA, Ribosomal, 16S/genetics ; Rodentia/blood ; Rodentia/microbiology
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2019-01-12
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2541872-5
    ISSN 1877-9603 ; 1877-959X
    ISSN (online) 1877-9603
    ISSN 1877-959X
    DOI 10.1016/j.ttbdis.2019.01.002
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  8. Article ; Online: SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection

    Bashor, Laura / Gagne, Roderick B. / Bosco-Lauth, Angela / Bowen, Richard / Stenglein, Mark / VandeWoude, Sue

    bioRxiv

    Abstract: SARS-CoV-2 spillback from humans into domestic and wild animals has been well-documented. We compared variants of cell culture-expanded SARS-CoV-2 inoculum and virus recovered from four species following experimental exposure. Five nonsynonymous changes ... ...

    Abstract SARS-CoV-2 spillback from humans into domestic and wild animals has been well-documented. We compared variants of cell culture-expanded SARS-CoV-2 inoculum and virus recovered from four species following experimental exposure. Five nonsynonymous changes in nsp12, S, N and M genes were near fixation in the inoculum, but reverted to wild-type sequences in RNA recovered from dogs, cats and hamsters within 1-3 days post-exposure. Fourteen emergent variants were detected in viruses recovered from animals, including substitutions at spike positions H69, N501, and D614, which also vary in human lineages of concern. The rapidity of in vitro and in vivo SARS-CoV-2 selection reveals residues with functional significance during host-switching, illustrating the potential for spillback reservoir hosts to accelerate evolution, and demonstrating plasticity of viral adaptation in animal models.
    Keywords covid19
    Language English
    Publishing date 2021-03-08
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2021.03.05.434135
    Database COVID19

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  9. Article: The Density of the Lyme Disease Vector Ixodes scapularis (Blacklegged Tick) Differs between the Champlain Valley and Green Mountains, Vermont

    Allen, David / Borgmann-Winter, Benjamin / Bashor, Laura / Ward, Jeremy

    Northeastern naturalist. 2019 July 24, v. 26, no. 3

    2019  

    Abstract: Lyme disease is an emerging infectious disease of public health concern in the northeastern US. The disease's vector, Ixodes scapularis (Blacklegged Tick), has increased its range in the past 20 y. There have been few studies of the Blacklegged Tick's ... ...

    Abstract Lyme disease is an emerging infectious disease of public health concern in the northeastern US. The disease's vector, Ixodes scapularis (Blacklegged Tick), has increased its range in the past 20 y. There have been few studies of the Blacklegged Tick's habitat associations in its newly endemic northern range. From 2016–2018, we sampled for nymphal Blacklegged Ticks in the Champlain Valley and Green Mountains of Addison County, VT, and tested them for Borrelia burgdorferi, the Lyme disease agent. We found 10 times more ticks in the Champlain Valley than in the Green Mountains. Nymphal infection prevalence was 0.21 and did not vary by year or region. The difference in tick density reported has public health consequences, as Vermont has one of the highest rates of Lyme disease in the US.
    Keywords Borrelia burgdorferi ; Ixodes scapularis ; Lyme disease ; disease vectors ; emerging diseases ; habitats ; mountains ; public health ; ticks ; Vermont
    Language English
    Dates of publication 2019-0724
    Size p. 545-560.
    Publishing place Humboldt Field Research Institute
    Document type Article
    ZDB-ID 2117206-7
    ISSN 1938-5307 ; 1092-6194
    ISSN (online) 1938-5307
    ISSN 1092-6194
    DOI 10.1656/045.026.0307
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: Let-7b-5p in vesicles secreted by human airway cells reduces biofilm formation and increases antibiotic sensitivity of

    Koeppen, Katja / Nymon, Amanda / Barnaby, Roxanna / Bashor, Laura / Li, Zhongyou / Hampton, Thomas H / Liefeld, Amanda E / Kolling, Fred W / LaCroix, Ian S / Gerber, Scott A / Hogan, Deborah A / Kasetty, Swetha / Nadell, Carey D / Stanton, Bruce A

    Proceedings of the National Academy of Sciences of the United States of America

    2021  Volume 118, Issue 28

    Abstract: Pseudomonas ... ...

    Abstract Pseudomonas aeruginosa
    MeSH term(s) Antagomirs/pharmacology ; Anti-Bacterial Agents/pharmacology ; Aztreonam/pharmacology ; Biofilms/drug effects ; Biofilms/growth & development ; Extracellular Vesicles/drug effects ; Extracellular Vesicles/metabolism ; Gene Expression Regulation, Bacterial/drug effects ; Humans ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Plankton/drug effects ; Pseudomonas aeruginosa/drug effects ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/isolation & purification ; Pseudomonas aeruginosa/physiology ; beta-Lactams/pharmacology
    Chemical Substances Antagomirs ; Anti-Bacterial Agents ; MicroRNAs ; beta-Lactams ; mirnlet7 microRNA, human ; Aztreonam (G2B4VE5GH8)
    Language English
    Publishing date 2021-07-06
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2105370118
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