Result 1 - 10 of total 215
Experimental BoDV-1 infection in Tamiops swinhoei: [Poster]
2024
| Keywords | Text ; abstract_or_summary ; ddc:570 |
|---|---|
| Language | English |
| Publishing date | 2024-03-25 |
| Publishing country | de |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
[Poster]
2024
| Keywords | Text ; abstract_or_summary ; ddc:610 |
|---|---|
| Language | English |
| Publishing date | 2024-03-25 |
| Publishing country | de |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
Abschlussbericht erste Förderphase 2017-2020 ; Final report first funding phase 2017 - 2020: Zoonotic Bornavirus Consortium (ZooBoCo)
2021
| Keywords | Text ; ddc:570 ; Project ZooBoCo |
|---|---|
| Language | German |
| Publishing date | 2021-10-13 |
| Publisher | Friedrich-Loeffler-Institut |
| Publishing country | de |
| Document type | Book ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
molecular detection and characterization
2017
Abstract: Encephalitis is a severe inflammatory disease of the brain which often has a fatal outcome or can lead to subsequent damages. In around two-thirds of all human encephalitis cases, the causative agent is, despite improved diagnostics, unknown today. Aim ... ...
| Abstract | Encephalitis is a severe inflammatory disease of the brain which often has a fatal outcome or can lead to subsequent damages. In around two-thirds of all human encephalitis cases, the causative agent is, despite improved diagnostics, unknown today. Aim of this work was the development, improvement and validation of diagnostic methods, improvement of sampling strategies and the development of optimized systems for characterization of three viruses causing viral encephalitis. The main burden of RABV lies in developing countries, were standard diagnostic tools are often not realizable. Therefore, simple and rapid diagnostic tests for the use under resource limited settings, so called point-of-care tests (POCT), are favorable. Commercially available lateral flow device (LFD) based immunodiagnostic tests were analyzed and failed in terms of sensitivity compared to the standard FAT and RT-qPCR (Paper I). Therefore, molecular RABV alternative targeting genome tests were developed and combined with rapid nucleic acid extraction methods. The new HighSpeed RT-qPCR and RPA assays together with magnetic bead based automated or manual extraction methods delivered a specificity between 100% and 97.2% and a limit of detection of 10 or 1,000 genome copies per reaction, respectively and seem suitable as novel POCT (Paper II). Recently, a novel zoonotic VSBV-1, responsible for fatal encephalitis of three squirrel breeders, was detected. For further investigations of this new virus, methods . Die Enzephalitis ist eine entzündliche Erkrankung des Gehirns, die oftmals tödlich verläuft oder Folgeschäden hervorruft. Trotz verbesserter diagnostischer Methoden wird auch gegenwärtig bei zwei Drittel aller Enzephalitiden die Ursache nicht ermittelt. Ziel dieser Arbeit war es, für drei mit Enzephalitis im Zusammenhang stehende Viren Nachweisverfahren zu entwickeln, zu verbessern und zu validieren, Beprobungsmethoden zu verbessern und Systems für die Viruscharakterisierung zu optimieren. Die Standardmethoden der Tollwut-Diagnostik kommen ... |
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| Keywords | Text ; ddc:570 ; Encephalitis ; Bornavirus ; Tollwutvirus ; Astrovirus |
| Subject code | 801 |
| Language | German |
| Publishing date | 2017-07-26 |
| Publisher | Ernst-Moritz-Arndt-Universität Greifswald, Mathematisch-Naturwissenschaftliche Fakultät |
| Publishing country | de |
| Document type | Thesis ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
Abschlussbericht erste Förderphase 2017-2020 : Verbundprojekt ; Abschlussbericht erste Förderphase 2017 - 2020: Zoonotic Bornavirus Consortium (ZooBoCo) ; Final report first funding phase 2017 - 2020: Zoonotic Bornavirus Consortium (ZooBoCo)
2021
Abstract: This subproject, collaborated with the FLI, based its work on the hypothesis that the clinical course and pathogenesis of both zoonotic and non-zoonotic bornaviruses in reservoir species (e.g., squirrels and shrews) is significantly different from that ... ...
| Abstract | This subproject, collaborated with the FLI, based its work on the hypothesis that the clinical course and pathogenesis of both zoonotic and non-zoonotic bornaviruses in reservoir species (e.g., squirrels and shrews) is significantly different from that of accidental hosts (dead-end hosts such as horses and humans). Reservoir hosts were expected to pass on the virus without becoming ill, whereas accidental hosts developed a strictly neurotropic infection with severe fatal non-sedative encephalitis. Furthermore, it was hypothesized that each bornavirus is specifically adapted to its reservoir host. To test these hypotheses, the establishment of viral persistence, viral release and adaptation, and the cellular response after infection with bornaviruses should be compared in in vitro as well as in vivo of reservoir and accidental host species. The Friedrich-Loeffler-Institute performed several animal experiments with VSBV-1 during the first funding phase of the ZooBoCo project (Lewis rats, long-tailed mice, bank voles, guinea pigs, gerbils, rabbits). The histopathological evaluation of these animal experiments was the responsibility of Justus Liebig University. Histologically, mild encephalitis was detected in isolated Lewis rats. In addition, the tissue distribution of naturally VSBV-1 infected squirrels was evaluated. Dieses mit dem FLI zusammenbearbeitete Teilprojekt legte seinen Arbeiten die Hypothese zugrunde, dass der klinische Verlauf und die Pathogenese sowohl zoonotischer als auch nicht-zoonotischer Bornaviren bei Reservoirspezies (z.B. Hörnchen und Spitzmäuse) signifikant unterschiedlich gegenüber akzidentiellen Wirten (Fehlwirten wie Pferd und Mensch) ist. Es wurde erwartet, dass Reservoirwirte das Virus weitergeben ohne zu erkranken, während akzidentielle Wirte eine strikt neurotrope Infektion mit schwerer fataler, nicht-eitriger Enzephalitis entwickeln. Weiterhin wurde vermutet, dass jedes Bornavirus spezifisch an seinen Reservoirwirt angepasst ist. Um diese Hypothesen zu überprüfen sollte die ... |
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| Keywords | Text ; ddc:570 ; Bornavirus ; Infektionskrankheiten ; parasitäre Krankheiten ; Medicine |
| Language | German |
| Publishing date | 2021-10-13 |
| Publisher | [Friedrich-Loeffler-Institut] |
| Publishing country | de |
| Document type | Book ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
2022
| Keywords | Text ; abstract_or_summary ; ddc:570 ; Project ZooBoCo |
|---|---|
| Language | German |
| Publishing date | 2022-07-11 |
| Publishing country | de |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
2022 Volume 14, Issue 2
Abstract: African swine fever (ASF) is a major threat to pig production, and real-time PCR (qPCR) protocols are an integral part of ASF laboratory diagnosis. With the pandemic spread of ASF, commercial kits have risen on the market. In Germany, the kits have to go ...
| Abstract | African swine fever (ASF) is a major threat to pig production, and real-time PCR (qPCR) protocols are an integral part of ASF laboratory diagnosis. With the pandemic spread of ASF, commercial kits have risen on the market. In Germany, the kits have to go through an approval process and thus, general validation can be assumed. However, they have never been compared to each other. In this study, 12 commercial PCR kits were compared to an OIE-recommended method. Samples representing different matrices, genome loads, and genotypes were included in a panel that was tested under diagnostic conditions. The comparison included user-friendliness, internal controls, and the time required. All qPCRs were able to detect ASFV genome in different matrices across all genotypes and disease courses. With one exception, there were no significant differences when comparing the overall mean. The overall specificity was 100% (95% CI 87.66-100), and the sensitivity was between 95% and 100% (95% CI 91.11-100). As can be expected, variability concerned samples with low genome load. To conclude, all tests were fit for purpose. The test system can therefore be chosen based on compatibility and prioritization of the internal control system. |
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| MeSH term(s) | African Swine Fever/diagnosis ; African Swine Fever/virology ; African Swine Fever Virus/classification ; African Swine Fever Virus/genetics ; African Swine Fever Virus/isolation & purification ; Animal Husbandry/organization & administration ; Animals ; DNA, Viral/genetics ; Genome, Viral ; Genotype ; Germany ; Reagent Kits, Diagnostic ; Real-Time Polymerase Chain Reaction/instrumentation ; Real-Time Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Swine ; World Health Organization |
| Chemical Substances | DNA, Viral ; Reagent Kits, Diagnostic |
| Language | English |
| Publishing date | 2022-01-24 |
| Publishing country | Switzerland |
| Document type | Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2516098-9 |
| ISSN | 1999-4915 ; 1999-4915 |
| ISSN (online) | 1999-4915 |
| ISSN | 1999-4915 |
| DOI | 10.3390/v14020220 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
molecular detection and characterization
2017
| Author's details | Kore Irina Jasmin Schlottau |
|---|---|
| Keywords | Biowissenschaften, Biologie ; Life Science, Biology |
| Subject code | sg570 |
| Language | English |
| Publisher | Universitätsbibliothek Greifswald |
| Publishing place | Greifswald |
| Document type | Book ; Online ; Thesis |
| Database | Digital theses on the web |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
Viruses. 2022 Jan. 24, v. 14, no. 2
2022
Abstract: African swine fever (ASF) is a major threat to pig production, and real-time PCR (qPCR) protocols are an integral part of ASF laboratory diagnosis. With the pandemic spread of ASF, commercial kits have risen on the market. In Germany, the kits have to go ...
| Abstract | African swine fever (ASF) is a major threat to pig production, and real-time PCR (qPCR) protocols are an integral part of ASF laboratory diagnosis. With the pandemic spread of ASF, commercial kits have risen on the market. In Germany, the kits have to go through an approval process and thus, general validation can be assumed. However, they have never been compared to each other. In this study, 12 commercial PCR kits were compared to an OIE-recommended method. Samples representing different matrices, genome loads, and genotypes were included in a panel that was tested under diagnostic conditions. The comparison included user-friendliness, internal controls, and the time required. All qPCRs were able to detect ASFV genome in different matrices across all genotypes and disease courses. With one exception, there were no significant differences when comparing the overall mean. The overall specificity was 100% (95% CI 87.66–100), and the sensitivity was between 95% and 100% (95% CI 91.11–100). As can be expected, variability concerned samples with low genome load. To conclude, all tests were fit for purpose. The test system can therefore be chosen based on compatibility and prioritization of the internal control system. |
|---|---|
| Keywords | African swine fever ; African swine fever virus ; laboratory diagnosis ; markets ; pandemic ; prioritization ; quantitative polymerase chain reaction ; swine production ; viral genome ; Germany |
| Language | English |
| Dates of publication | 2022-0124 |
| Publishing place | Multidisciplinary Digital Publishing Institute |
| Document type | Article |
| ZDB-ID | 2516098-9 |
| ISSN | 1999-4915 |
| ISSN | 1999-4915 |
| DOI | 10.3390/v14020220 |
| Database | NAL-Catalogue (AGRICOLA) |
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
2018
| Keywords | Text ; ddc:630 ; Afrikanische Schweinepest -- Diagnostik |
|---|---|
| Language | German |
| Publishing date | 2018-12-21 |
| Publishing country | de |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.