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Article ; Online: Acid-Sensing Ion Channels' Immunoreactivity in Nerve Profiles and Glomus Cells of the Human Carotid Body.

Martínez-Barbero, Graciela / García-Mesa, Yolanda / Cobo, Ramón / Cuendias, Patricia / Martín-Biedma, Benjamín / García-Suárez, Olivia / Feito, Jorge / Cobo, Teresa / Vega, José A

International journal of molecular sciences

2023 Volume 24, Issue 24

Abstract: The carotid body is a major peripheral chemoreceptor that senses changes in arterial blood oxygen, carbon dioxide, and pH, which is important for the regulation of breathing and cardiovascular function. The mechanisms by which the carotid body senses ... ...

Abstract	The carotid body is a major peripheral chemoreceptor that senses changes in arterial blood oxygen, carbon dioxide, and pH, which is important for the regulation of breathing and cardiovascular function. The mechanisms by which the carotid body senses O
MeSH term(s)	Humans ; Acid Sensing Ion Channels/metabolism ; Carotid Body/metabolism ; Chemoreceptor Cells/metabolism ; Peripheral Nervous System/metabolism ; Acidosis/metabolism
Chemical Substances	Acid Sensing Ion Channels
Language	English
Publishing date	2023-12-05
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms242417161
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Article ; Online: Comprehensive analysis of current epidemiology, clinical features and prognostic factors of puerperal endometritis: A retrospective cohort analysis.

San-Juan, Rafael / Sanz-Prieto, Alba / Contreras-Mora, Javier / Fojo-Suárez, Iván / Caso-Laviana, José María / Fernández-Ruiz, Mario / López-Medrano, Francisco / Rodríguez-Goncer, Isabel / Fayos, Marina / Brañas, Patricia / Barbero Casado, Patricia / García-Burguillo, Antonio / Aguado, José María

European journal of obstetrics & gynecology and reproductive biology: X

2023 Volume 18, Page(s) 100199

Abstract: Background: Puerperal endometritis has not been recently investigated. We aimed to describe the current dimension of the endometritis in the context of other causes of puerperal fever and investigate the microbiology and need for curettage in these ... ...

Abstract	Background: Puerperal endometritis has not been recently investigated. We aimed to describe the current dimension of the endometritis in the context of other causes of puerperal fever and investigate the microbiology and need for curettage in these patients. Methods: A retrospective cohort study was conducted based on a prospectively maintained database of patients with puerperal fever, (2014-2020) in which cases fulfilling criteria for endometritis were selected for further analysis. Description of clinical and microbiological features was performed and determination of the factors related with puerperal curettage requirement were studied using univariate and multivariate analysis through binary logistic regression. Results: From 428 patients with puerperal fever, endometritis was the main cause of puerperal fever (233 patients, 52.7 %). Curettage was required in 96 of them (41.2 %). Culture of endometrial samples were performed in 62 (64.5 %), of which 32 (51.6 %) yielded bacterial growth. Conclusions: Endometritis is still the main cause of puerperal fever. Women requiring curettage typically presented with abdominal pain and foul-smelling lochia, an ultrasound image compatible with RPOC and fever in the first 14 days postpartum. Curettage culture is useful for the microbiological affiliation mostly yielding gram-negative enteric flora.
Language	English
Publishing date	2023-05-16
Publishing country	Netherlands
Document type	Journal Article
ISSN	2590-1613
ISSN (online)	2590-1613
DOI	10.1016/j.eurox.2023.100199
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Article ; Online: Evaluation of the humoral response to the third dose of SARS-COV-2 vaccines in liver transplant recipients.

Ducasa, Nicolás / Benencio, Paula / Mauro, Ezequiel / Anders, Margarita / Mazzitelli, Bianca / Bleichmar, Lucía / Quiroga, Maria Florencia / Barbero, Manuel / Cairo, Fernando / Alter, Adriana / Etcheves, Patricia / Scarton, Giampaolo / Sobenko, Natalia / Biglione, Mirna / Mendizabal, Manuel

Vaccine

2023 Volume 41, Issue 52, Page(s) 7663–7670

Abstract: Solid organ transplant recipients (SOTR) commonly develop an unsatisfactory humoral response to vaccines compared to immunocompetent individuals (IC). We have previously evaluated the humoral response in liver transplant recipients (LTR) who received two- ...

Abstract	Solid organ transplant recipients (SOTR) commonly develop an unsatisfactory humoral response to vaccines compared to immunocompetent individuals (IC). We have previously evaluated the humoral response in liver transplant recipients (LTR) who received two-dose vaccines against SARS-CoV-2 and reported that 38 % of LTR did not produce anti-Spike antibodies. Thus, we set out to evaluate the humoral response after the third dose of SARS-CoV-2 vaccines. For this purpose, samples from a cohort of 81 LTR and 27 IC were extracted between 21 and 90 days after the third dose. Serology for anti-Spike IgG antibodies and neutralizing antibodies against Wuhan, Delta and Omicron variants were evaluated. We found that 73.5 % of LTR were responders for anti-Spike IgG, while all the IC mounted a measurable response. LTR who responded to the third dose showed significantly lower anti-Spike IgG levels and neutralizing antibodies than IC. We found that there is less neutralization in LTR compared to IC across all variants. Specifically, the neutralization titers in both groups decrease when encountering the Delta variant, and this decline is even more pronounced with the Omicron variant, compared to the Wuhan variant. Furthermore, we identified that the use of high doses of mycophenolate and advanced age were factors that negatively affected the development of anti-Spike IgG antibodies. Regarding vaccine regimes, the regime viral vector/mRNA/mRNA elicited significantly higher responses in LTR compared to other vaccine schemes. In addition to the recommended and necessary booster doses in this population, strategies that achieve adequate immunization should be evaluated.
MeSH term(s)	Humans ; COVID-19 Vaccines ; Liver Transplantation ; COVID-19/prevention & control ; SARS-CoV-2 ; Antibodies, Neutralizing ; RNA, Messenger ; Transplant Recipients ; Immunoglobulin G ; Antibodies, Viral
Chemical Substances	COVID-19 Vaccines ; Antibodies, Neutralizing ; RNA, Messenger ; Immunoglobulin G ; Antibodies, Viral
Language	English
Publishing date	2023-11-25
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2023.11.039
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Article ; Online: Acid-Sensing Ion Channels’ Immunoreactivity in Nerve Profiles and Glomus Cells of the Human Carotid Body

Graciela Martínez-Barbero / Yolanda García-Mesa / Ramón Cobo / Patricia Cuendias / Benjamín Martín-Biedma / Olivia García-Suárez / Jorge Feito / Teresa Cobo / José A. Vega

International Journal of Molecular Sciences, Vol 24, Iss 24, p

2023 Volume 17161

Abstract	The carotid body is a major peripheral chemoreceptor that senses changes in arterial blood oxygen, carbon dioxide, and pH, which is important for the regulation of breathing and cardiovascular function. The mechanisms by which the carotid body senses O 2 and CO 2 are well known; conversely, the mechanisms by which it senses pH variations are almost unknown. Here, we used immunohistochemistry to investigate how the human carotid body contributes to the detection of acidosis, analyzing whether it expresses acid-sensing ion channels (ASICs) and determining whether these channels are in the chemosensory glomic cells or in the afferent nerves. In ASIC1, ASIC2, and ASIC3, and to a much lesser extent ASIC4, immunoreactivity was detected in subpopulations of type I glomus cells, as well as in the nerves of the carotid body. In addition, immunoreactivity was found for all ASIC subunits in the neurons of the petrosal and superior cervical sympathetic ganglia, where afferent and efferent neurons are located, respectively, innervating the carotid body. This study reports for the first time the occurrence of ASIC proteins in the human carotid body, demonstrating that they are present in glomus chemosensory cells (ASIC1 < ASIC2 > ASIC3 > ASIC4) and nerves, presumably in both the afferent and efferent neurons supplying the organ. These results suggest that the detection of acidosis by the carotid body can be mediated via the ASIC ion channels present in the type I glomus cells or directly via sensory nerve fibers.
Keywords	carotid body ; glomus cells ; nerves ; acid-sensing ion channels ; immunohistochemistry ; human ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Depression, anxiety, burnout and empathy among Spanish medical students.

Capdevila-Gaudens, Patricia / García-Abajo, J Miguel / Flores-Funes, Diego / García-Barbero, Mila / García-Estañ, Joaquín

PloS one

2021 Volume 16, Issue 12, Page(s) e0260359

Abstract: Medical Education studies suggest that medical students experience mental distress in a proportion higher than in the rest of the population In the present study, we aimed to conduct a nationwide analysis of the prevalence of mental health problems among ...

Abstract	Medical Education studies suggest that medical students experience mental distress in a proportion higher than in the rest of the population In the present study, we aimed to conduct a nationwide analysis of the prevalence of mental health problems among medical students. The study was carried out in 2020 in all 43 medical schools in Spain, and analyzes the prevalence of depression, anxiety, empathy and burnout among medical students (n = 5216). To measure these variables we used the Beck Depression Inventory Test for assessing depression, the Maslach Burnout Inventory Survey for Students was used for burnout, the State-Trait Anxiety Inventory (STAI) was used to assess anxiety state and trait and the Jefferson Empathy Scale 12 to obtain empathy scores. In relation to depression, the data indicate an overall prevalence of 41%, with 23.4% of participants having moderate to severe levels, and 10% experiencing suicidal ideation. Burnout prevalence was 37%, significantly higher among 6th year than among 1st year students. Anxiety levels were consistent with those reported previously among medical students (25%), and were higher than in the general population for both trait and state anxiety. The prevalence of trait anxiety was higher among women. Empathy scores were at the top end of the scale, with the highest-scoring group (>130) containing a greater percentage of women. Similarly to those published previously for other countries, these results provide a clear picture of the mental disorders affecting Spanish medical students. Medicine is an extremely demanding degree and it is important that universities and medical schools view this study as an opportunity to ensure conditions that help minimize mental health problems among their students. Some of the factors underlying these problems can be prevented by, among other things, creating an environment in which mental health is openly discussed and guidance is provided. Other factors need to be treated medically, and medical schools and universities should therefore provide support to students in need through the medical services available within their institutions.
MeSH term(s)	Adult ; Depression ; Empathy ; Female ; Humans ; Male ; Students, Medical
Language	English
Publishing date	2021-12-02
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0260359
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Article ; Online: Synthesis of Polysubstituted Tetrahydropyrans by Stereoselective Hydroalkoxylation of Silyl Alkenols: En Route to Tetrahydropyranyl Marine Analogues.

Díez-Poza, Carlos / Val, Patricia / Pulido, Francisco J / Barbero, Asunción

Marine drugs

2018 Volume 16, Issue 11

Abstract: Tetrahydropyrans are abundantly found in marine natural products. The interesting biological properties of these compounds and their analogues make necessary the development of convenient procedures for their synthesis. In this paper, an atom economy ... ...

Abstract	Tetrahydropyrans are abundantly found in marine natural products. The interesting biological properties of these compounds and their analogues make necessary the development of convenient procedures for their synthesis. In this paper, an atom economy access to tetrahydropyrans by intramolecular acid-mediated cyclization of silylated alkenols is described. p-TsOH has shown to be an efficient reagent to yield highly substituted tetrahydropyrans. Moreover, excellent diastereoselectivities are obtained both for unsubstituted and alkylsubstituted vinylsilyl alcohols. The methodology herein developed may potentially be applied to the synthesis of marine drugs derivatives.
MeSH term(s)	Acids/chemistry ; Alcohols/chemistry ; Alkenes/chemistry ; Aquatic Organisms/chemistry ; Biological Products/chemistry ; Catalysis ; Cyclization ; Molecular Structure ; Pyrans/chemical synthesis ; Pyrans/chemistry ; Stereoisomerism
Chemical Substances	Acids ; Alcohols ; Alkenes ; Biological Products ; Pyrans
Language	English
Publishing date	2018-11-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2175190-0
ISSN	1660-3397 ; 1660-3397
ISSN (online)	1660-3397
ISSN	1660-3397
DOI	10.3390/md16110421
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Article: Biomarkers of Immunotherapy Response in Patients with Non-Small-Cell Lung Cancer: Microbiota Composition, Short-Chain Fatty Acids, and Intestinal Permeability.

Moratiel-Pellitero, Alba / Zapata-García, María / Gascón-Ruiz, Marta / Sesma, Andrea / Quílez, Elisa / Ramirez-Labrada, Ariel / Martínez-Lostao, Luis / Domingo, María Pilar / Esteban, Patricia / Yubero, Alfonso / Barbero-Herranz, Raquel / Moreno-Blanco, Ana / Paño, José Ramón / Lastra, Rodrigo / Pardo, Julián / Isla, Dolores / Del Campo, Rosa / Gálvez, Eva

Cancers

2024 Volume 16, Issue 6

Abstract: Immune checkpoint inhibitors have been proposed as the standard treatment for different stages of non-small-cell lung cancer in multiple indications. Not all patients benefit from these treatments, however, and certain patients develop immune-related ... ...

Abstract	Immune checkpoint inhibitors have been proposed as the standard treatment for different stages of non-small-cell lung cancer in multiple indications. Not all patients benefit from these treatments, however, and certain patients develop immune-related adverse events. Although the search for predictors of response to these drugs is a major field of research, these issues have yet to be resolved. It has been postulated that microbiota could play a relevant role in conditioning the response to cancer treatments; however, the human factor of intestinal permeability also needs to be considered as it is closely related to the regulation of host-microbiota interaction. In this article, we analyzed the possible relationship between the response to immune checkpoint inhibitors and the onset of immune-related adverse events, gut microbiota status, and intestinal membrane permeability. In a pioneering step, we also measured short-chain fatty acid content in feces. Although the correlation analyses failed to identify predictive biomarkers, even when all variables were integrated, our patients' microbial gut ecosystems were rich and diverse, and the intestinal barrier's integrity was preserved. These results add new knowledge on the composition of microbiota and its correlation with barrier permeability and short-chain fatty acids and suggest that more studies are required before these potential biomarkers can be incorporated into the clinical management of patients via immune checkpoint inhibitor treatment.
Language	English
Publishing date	2024-03-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers16061144
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Article: Diaphyseal Radius Fracture Associated with Irreducible Head Dislocation. Case Report

Blanco, Alfredo Villar / Barbero, Patricia Gómez / Aparicio, María Del Sol Gómez / Correa, Jose Ignacio Pérez

Revista Iberoamericana de Cirugía de la Mano

2021 Volume 49, Issue 02, Page(s) e155–e159

Abstract: Irreducible dislocation of the radial head is an extremely rare lesion, especially in an adult patient. We present a case of diaphyseal radius fracture associated with a posterior elbow dislocation and an irreducible radial head dislocation. After closed ...

Abstract	Irreducible dislocation of the radial head is an extremely rare lesion, especially in an adult patient. We present a case of diaphyseal radius fracture associated with a posterior elbow dislocation and an irreducible radial head dislocation. After closed reduction of the elbow, we performed open reduction and ostheosynthesis of the radius, and the radial head remained irreducible. We finally found, surrounding the radius, the interposition of the insertion of the biceps, and, after extracting it, we performed the correct reduction of the radial head. Six months after the surgery, the patient presented a full articular balance, with no pain. We have not found any similar case in the literature. La luxación no reductible de cabeza radial es una lesión extremadamente infrecuente, especialmente en el paciente adulto. Presentamos el caso de una fractura diafisaria de radio asociada a una luxación posterior de codo y a una luxación irreductible de la cabeza radial. Tras una reducción cerrada de codo, se realizó la reducción abierta y osteosíntesis de radio, permaneciendo irreductible la cabeza radial. Finalmente hallamos circundando al radio la interposición de la inserción del bíceps, y, tras la retirada de la misma, realizamos la correcta reducción de la cabeza radial. Seis meses tras la cirugía, el paciente presentaba un balance articular completo sin dolor. No hemos hallado en la literatura ningún caso similar.
Keywords	radial head dislocation ; articular fracture ; radius fracture ; biceps tendon ; luxación de la cabeza del radio ; fractura articular ; fractura del radio ; tendón del bíceps
Language	English
Publishing date	2021-11-01
Publisher	Thieme Revinter Publicações Ltda.
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	2874929-7
ISSN	1698-840X ; 1698-8396
ISSN (online)	1698-840X
ISSN	1698-8396
DOI	10.1055/s-0041-1731768
Database	Thieme publisher's database

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Article ; Online: A "Before and After" in the Use of Emergency Services in Spain? The Impact of the Economic Crisis.

Pereira, Patricia López / Casanova, Ana Pagá / Sanz-Barbero, Belén

International journal of health services : planning, administration, evaluation

2016 Volume 46, Issue 3, Page(s) 430–447

Abstract: The objective of this study was to analyse whether variables associated with emergency services (ES) use in Spain have changed in the period 2006-2011 using a comparative analysis of the 2006 and 2011 Spanish National Health Surveys. The measure of ... ...

Abstract	The objective of this study was to analyse whether variables associated with emergency services (ES) use in Spain have changed in the period 2006-2011 using a comparative analysis of the 2006 and 2011 Spanish National Health Surveys. The measure of association was the prevalence ratio with its 95% confidence interval, obtained by Poisson regression with robust variance. We studied interactions between the explanatory variables for ES use and year and subsequently performed a stratified analysis by year. ES use declined by 2.1% in 2011. Most emergency care (approximately 60% in both surveys) was provided in public hospitals. Between 2006 and 2011, ES use increased by 9% in women (p for interaction = 0.008) [ref. men], 3% in persons with poor mental health (p for interaction = 0.072) [ref. good mental health], and 8% in individuals with limitations on activities of daily living (p for interaction = 0.006) [ref. no limitations]. The change in the effect of the variables sex, mental health, and limitations on activities of daily living on use of ES (2006 and 2011) is not explained by either demographic characteristics or individual socioeconomic indicators. These results could be associated with macroeconomic and structural changes occurring during the period 2006-2011.
MeSH term(s)	Adolescent ; Adult ; Aged ; Cross-Sectional Studies ; Economic Recession ; Emergency Medical Services/statistics & numerical data ; Emergency Medical Services/trends ; Female ; Health Status ; Health Surveys ; Humans ; Male ; Middle Aged ; Sex Factors ; Socioeconomic Factors ; Spain/epidemiology ; Surveys and Questionnaires ; Unemployment ; Young Adult
Language	English
Publishing date	2016
Publishing country	United States
Document type	Journal Article
ZDB-ID	184936-0
ISSN	1541-4469 ; 0020-7314
ISSN (online)	1541-4469
ISSN	0020-7314
DOI	10.1177/0020731416636591
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Article: Prevalence of non-alcoholic fatty liver disease and liver fibrosis in a general population cohort from Argentina.

Villamil, Federico Guillermo / Barbero, Manuel / Massenzio, Nancy Elena / Giani, Sandra Mónica / Kozima, Shigeru / Cairo, Fernando Mario / Belloni, Rodrigo Agustín / Mariani, Javier / Gazari, Mercedes Rodriguez / Coisson, Paola Evangelina / Gallardo, Sonia Carolina / Cocco, Paula Andrea / Riboldi, Claudia Mabel / Gallardo, Patricia Eugenia

Annals of hepatology

2023 Volume 28, Issue 4, Page(s) 101111

Abstract: Introduction and objectives: South America is one of the regions with the highest rates of non-alcoholic fatty liver disease (NAFLD). This study aimed to assess the prevalence and severity of NAFLD in suburban Argentina.: Patients and methods: The ... ...

Abstract	Introduction and objectives: South America is one of the regions with the highest rates of non-alcoholic fatty liver disease (NAFLD). This study aimed to assess the prevalence and severity of NAFLD in suburban Argentina. Patients and methods: The study involved a general community cohort of 993 subjects evaluated sequentially with a comprehensive lifestyle questionnaire, laboratory testing, abdominal ultrasound (US) and transient elastography with XL probe. NAFLD was diagnosed according to standard criteria. Results: The prevalence of NAFLD by the US was 37.2% (326/875) overall, 50.3% in subjects with overweight/obesity, 58.6% with hypertriglyceridemia, 62.3% with diabetes/hyperglycemia and 72.1% with all three risk factors. Male gender (OR 1.42, 95% CI 1.03-1.47, p = 0.029), age (50-59 years: OR 1.98, 95 CI 1.16-3.39, p = 0.013 and ≥60 years: OR 1.86, 95% CI 1.13-3.09, p = 0.015), BMI (25-29: OR 2.87, 95% CI 1.86-4.51, p<0.001 and ≥30: OR 9.57, 95% CI 6.14-15.20, p<0.001), diabetes/hyperglycemia (OR 1.65, 95% CI 1.05-2.61, p = 0.029) and hypertriglyceridemia (OR 1.73, 95% CI 1.20-2.48, p = 0.002) were independent predictors of NAFLD. Among patients with steatosis, 22.2% (69/311) had ≥F2 fibrosis (overweight 25%, hypertriglyceridemia 32%, diabetes/hyperglycemia 34%). BMI (OR 5.22, 95% CI 2.64-11.74, p<0.001), diabetes/hyperglycemia (OR 2.12, 95% CI 1.05-4.29, p = 0.04) and hypertriglyceridemia (OR 1.94, 95% CI 1.03-3.68, p = 0.040) were independent predictors of liver fibrosis. Conclusions: This general population study from Argentina showed a high prevalence of NAFLD. Significant liver fibrosis was present in 22% of subjects with NAFLD. This information adds to the existing knowledge of NAFLD epidemiology in Latin America.
MeSH term(s)	Humans ; Male ; Middle Aged ; Non-alcoholic Fatty Liver Disease/diagnostic imaging ; Non-alcoholic Fatty Liver Disease/epidemiology ; Overweight ; Prevalence ; Argentina/epidemiology ; Liver Cirrhosis/diagnostic imaging ; Liver Cirrhosis/epidemiology ; Diabetes Mellitus/etiology ; Hyperglycemia/complications ; Hyperglycemia/pathology ; Hypertriglyceridemia/complications ; Hypertriglyceridemia/pathology ; Liver/pathology
Language	English
Publishing date	2023-04-25
Publishing country	Mexico
Document type	Journal Article
ZDB-ID	2188733-0
ISSN	1665-2681
ISSN	1665-2681
DOI	10.1016/j.aohep.2023.101111
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