Article ; Online: A generic pump-free organ-on-a-chip platform for assessment of intestinal drug absorption.
2024 Volume 19, Issue 2, Page(s) e2300390
Abstract: Organ-on-a-chip technology has shown great potential in disease modeling and drug evaluation. However, traditional organ-on-a-chip devices are mostly pump-dependent with low throughput, which makes it difficult to leverage their advantages. In this study, ...
| Abstract | Organ-on-a-chip technology has shown great potential in disease modeling and drug evaluation. However, traditional organ-on-a-chip devices are mostly pump-dependent with low throughput, which makes it difficult to leverage their advantages. In this study, we have developed a generic, pump-free organ-on-a-chip platform consisting of a 32-unit chip and an adjustable rocker, facilitating high-throughput dynamic cell culture with straightforward operation. By utilizing the rocker to induce periodic fluid forces, we can achieve fluidic conditions similar to those obtained with traditional pump-based systems. Through constructing a gut-on-a-chip model, we observed remarkable enhancements in the expression of barrier-associated proteins and the spatial distribution of differentiated intestinal cells compared to static culture. Furthermore, RNA sequencing analysis unveiled enriched pathways associated with cell proliferation, lipid transport, and drug metabolism, indicating the ability of the platform to mimic critical physiological processes. Additionally, we tested seven drugs that represent a range of high, medium, and low in vivo permeability using this model and found a strong correlation between their P |
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| MeSH term(s) | Humans ; Lab-On-A-Chip Devices ; Microphysiological Systems ; Cell Culture Techniques ; Intestines ; Biological Transport |
| Language | English |
| Publishing date | 2024-02-20 |
| Publishing country | Germany |
| Document type | Journal Article |
| ZDB-ID | 2221885-3 |
| ISSN | 1860-7314 ; 1860-6768 |
| ISSN (online) | 1860-7314 |
| ISSN | 1860-6768 |
| DOI | 10.1002/biot.202300390 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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