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  1. Article ; Online: SARS-CoV-2 infection in conjunctival tissue - Authors' reply.

    Hui, Kenrie Py / Peiris, Malik / Nicholls, J M / Chan, Michael Cw

    The Lancet. Respiratory medicine

    2020  Volume 8, Issue 7, Page(s) e58

    MeSH term(s) Betacoronavirus ; COVID-19 ; Conjunctiva ; Coronavirus Infections ; Humans ; Immunity, Innate ; Pandemics ; Pneumonia, Viral ; Respiratory System ; SARS-CoV-2 ; Tropism
    Keywords covid19
    Language English
    Publishing date 2020-07-09
    Publishing country England
    Document type Letter ; Comment
    ZDB-ID 2686754-0
    ISSN 2213-2619 ; 2213-2600
    ISSN (online) 2213-2619
    ISSN 2213-2600
    DOI 10.1016/S2213-2600(20)30273-3
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  3. Article ; Online: SARS-CoV-2 infection in conjunctival tissue – Authors' reply

    Hui, Kenrie PY / Peiris, Malik / Nicholls, JM / Chan, Michael CW

    The Lancet Respiratory Medicine

    2020  Volume 8, Issue 7, Page(s) e58

    Keywords Pulmonary and Respiratory Medicine ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 2686754-0
    ISSN 2213-2619 ; 2213-2600
    ISSN (online) 2213-2619
    ISSN 2213-2600
    DOI 10.1016/s2213-2600(20)30273-3
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 7041: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Mutation nsp6 L232F associated with MERS-CoV zoonotic transmission confers higher viral replication in human respiratory tract cultures ex-vivo.

    So, Ray Ty / Chu, Daniel Kw / Hui, Kenrie Py / Mok, Chris Kp / Sanyal, Sumana / Nicholls, John M / Ho, John C W / Cheung, Man-Chun / Ng, Ka-Chun / Yeung, Hin-Wo / Chan, Michael Cw / Poon, Leo Lm / Zhao, Jincun / Peiris, Malik

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Middle East respiratory syndrome coronavirus (MERS-CoV) causes zoonotic disease. Dromedary camels are the source of zoonotic infection. We identified a mutation of amino acid leucine to phenylalanine in the codon 232 position of the non-structural ... ...

    Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) causes zoonotic disease. Dromedary camels are the source of zoonotic infection. We identified a mutation of amino acid leucine to phenylalanine in the codon 232 position of the non-structural protein 6 (nsp6) (nsp6 L232F) that is repeatedly associated with zoonotic transmission. We generated a pair of isogenic recombinant MERS-CoV with nsp6 232L and 232F residues, respectively, and showed that the nsp6 L232F mutation confers higher replication competence in ex-vivo culture of human nasal and bronchial tissues and in lungs of mice experimentally infected in-vivo. Mechanistically, the nsp6 L232F mutation appeared to modulate autophagy and was associated with higher exocytic virus egress, while innate immune responses and zippering activity of the endoplasmic reticulum remained unaffected. Our study suggests that MERS-CoV nsp6 may contribute to viral adaptation to humans. This highlights the importance of continued surveillance of MERS-CoV in both camels and humans.
    Language English
    Publishing date 2023-03-28
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.03.27.534490
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: ACE2 nanoparticles prevent cell entry of SARS-CoV-2

    Sauvanet, Cecile / Lemos, Moara / Bezault, Armel / Rodriguez de Francisco, Borja / Chan, Michael CW / Hui, Kenrie PY / Ng, Ka-chun / Nicholls, John M / Volkmann, Niels / Hanein, Dorit

    bioRxiv

    Abstract: We have now been in the grip of the COVID-19 pandemic for over two years with devastating consequences. The continual evolution of the virus has challenged the efficacy of many vaccines and treatment options based on immunotherapies are compromised by ... ...

    Abstract We have now been in the grip of the COVID-19 pandemic for over two years with devastating consequences. The continual evolution of the virus has challenged the efficacy of many vaccines and treatment options based on immunotherapies are compromised by this viral escape. One treatment strategy that averts viral escape is the use of constructs based on its entry receptor Angiotensin-Converting Enzyme 2 (ACE2) acting as decoys. Here, we combined full-length human ACE2 with viral vectors commonly used for gene therapy to form nanoparticles that present ACE2 on their surface analogous to human cells. Using cell-based assays and direct, multiscale imaging including cryogenic cellular tomography, we show that these ACE2 nanoparticles are highly efficient in preventing entry of SARS-CoV-2, the virus causing COVID-19, in model cell systems as well as human respiratory tract ex-vivo cultures. Thus, ACE2 nanoparticles have high potential as the next generation therapeutics for addressing the immediate needs of the current pandemic and possible future outbreaks.
    Keywords covid19
    Language English
    Publishing date 2022-05-06
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2022.05.05.490805
    Database COVID19

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  6. Article ; Online: Tropism of the Novel Coronavirus SARS-CoV-2 in Human Respiratory Tract

    Hui, Kenrie PY / Cheung, Man-Chun / Perera, Ranawaka APM / Ng, Ka-Chun / Bui, Christine BT / Ho, John CW / Ng, Mandy MT / Kuok, Denise IT / Shih, Kendrick C. / Tsao, SW / Poon, Leo LM / Peiris, Malik / Nicholls, John M. / Chan, Michael CW

    SSRN Electronic Journal ; ISSN 1556-5068

    An Analysis in Ex Vivo and In Vitro Cultures

    2020  

    Keywords covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    DOI 10.2139/ssrn.3552870
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Replication of Novel Zoonotic-Like Influenza A(H3N8) Virus in Ex Vivo Human Bronchus and Lung

    Kenrie P.Y. Hui / John C.W. Ho / Ka-Chun Ng / Samuel M.S. Cheng / Ko-Yung Sit / Timmy W.K. Au / Leo L.M. Poon / John M. Nicholls / Malik Peiris / Michael C.W. Chan

    Emerging Infectious Diseases, Vol 29, Iss 6, Pp 1210-

    2023  Volume 1214

    Abstract: Human infection with avian influenza A(H3N8) virus is uncommon but can lead to acute respiratory distress syndrome. In explant cultures of the human bronchus and lung, novel H3N8 virus showed limited replication efficiency in bronchial and lung tissue ... ...

    Abstract Human infection with avian influenza A(H3N8) virus is uncommon but can lead to acute respiratory distress syndrome. In explant cultures of the human bronchus and lung, novel H3N8 virus showed limited replication efficiency in bronchial and lung tissue but had a higher replication than avian H3N8 virus in lung tissue.
    Keywords influenza ; H3N8 ; risk assessment ; human bronchus ; human lung ; influenza A virus ; Medicine ; R ; Infectious and parasitic diseases ; RC109-216
    Language English
    Publishing date 2023-06-01T00:00:00Z
    Publisher Centers for Disease Control and Prevention
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Risk Assessment for Highly Pathogenic Avian Influenza A(H5N6/H5N8) Clade 2.3.4.4 Viruses

    Christine H.T. Bui / Denise I.T. Kuok / Hin Wo Yeung / Ka-Chun Ng / Daniel K.W. Chu / Richard J. Webby / John M. Nicholls / J.S. Malik Peiris / Kenrie P.Y. Hui / Michael C.W. Chan

    Emerging Infectious Diseases, Vol 27, Iss 10, Pp 2619-

    2021  Volume 2627

    Abstract: The numerous global outbreaks and continuous reassortments of highly pathogenic avian influenza (HPAI) A(H5N6/H5N8) clade 2.3.4.4 viruses in birds pose a major risk to the public health. We investigated the tropism and innate host responses of 5 recent ... ...

    Abstract The numerous global outbreaks and continuous reassortments of highly pathogenic avian influenza (HPAI) A(H5N6/H5N8) clade 2.3.4.4 viruses in birds pose a major risk to the public health. We investigated the tropism and innate host responses of 5 recent HPAI A(H5N6/H5N8) avian isolates of clades 2.3.4.4b, e, and h in human airway organoids and primary human alveolar epithelial cells. The HPAI A(H5N6/H5N8) avian isolates replicated productively but with lower competence than the influenza A(H1N1)pdm09, HPAI A(H5N1), and HPAI A(H5N6) isolates from humans in both or either models. They showed differential cellular tropism in human airway organoids; some infected all 4 major epithelial cell types: ciliated cells, club cells, goblet cells, and basal cells. Our results suggest zoonotic potential but low transmissibility of the HPAI A(H5N6/H5N8) avian isolates among humans. These viruses induced low levels of proinflammatory cytokines/chemokines, which are unlikely to contribute to the pathogenesis of severe disease.
    Keywords influenza ; human airway organoids ; risk assessment ; tropism ; innate host responses ; HPAI H5Nx ; Medicine ; R ; Infectious and parasitic diseases ; RC109-216
    Subject code 610
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Centers for Disease Control and Prevention
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Replication of SARS-CoV-2 Omicron BA.2 variant in ex vivo cultures of the human upper and lower respiratory tract

    Kenrie P.Y. Hui / Ka-Chun Ng / John C.W. Ho / Hin-Wo Yeung / Rachel H.H. Ching / Haogao Gu / Joseph C.K. Chung / Velda L.Y. Chow / Ko-Yung Sit / Michael K.Y. Hsin / Timmy W.K. Au / Leo L.M. Poon / Malik Peiris / John M. Nicholls / Michael C.W. Chan

    EBioMedicine, Vol 83, Iss , Pp 104232- (2022)

    2022  

    Abstract: Summary: Background: The Omicron BA.2 sublineage has replaced BA.1 worldwide and has comparable levels of immune evasion to BA.1. These observations suggest that the increased transmissibility of BA.2 cannot be explained by the antibody evasion. Methods: ...

    Abstract Summary: Background: The Omicron BA.2 sublineage has replaced BA.1 worldwide and has comparable levels of immune evasion to BA.1. These observations suggest that the increased transmissibility of BA.2 cannot be explained by the antibody evasion. Methods: Here, we characterized the replication competence and respiratory tissue tropism of three Omicron variants (BA.1, BA.1.1, BA.2), and compared these with the wild-type virus and Delta variant, in human nasal, bronchial and lung tissues cultured ex vivo. Findings: BA.2 replicated more efficiently in nasal and bronchial tissues at 33°C than wild-type, Delta and BA.1. Both BA.2 and BA.1 had higher replication competence than wild-type and Delta viruses in bronchial tissues at 37°C. BA.1, BA.1.1 and BA.2 replicated at a lower level in lung parenchymal tissues compared to wild-type and Delta viruses. Interpretation: Higher replication competence of Omicron BA.2 in the human upper airway at 33°C than BA.1 may be one of the reasons to explain the current advantage of BA.2 over BA.1. A lower replication level of the tested Omicron variants in human lung tissues is in line with the clinical manifestations of decreased disease severity of patients infected with the Omicron strains compared with other ancestral strains. Funding: This work was supported by US National Institute of Allergy and Infectious Diseases and the Theme-Based Research Scheme under University Grants Committee of Hong Kong Special Administrative Region, China.
    Keywords SARS-CoV-2 ; Omicron BA.2 ; Nasal tissue ; Bronchial tissue ; Transmission ; Pathogenicity ; Medicine ; R ; Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2022-09-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Introduction of ORF3a-Q57H SARS-CoV-2 Variant Causing Fourth Epidemic Wave of COVID-19, Hong Kong, China

    Daniel K.W. Chu / Kenrie P.Y. Hui / Haogao Gu / Ronald L.W. Ko / Pavithra Krishnan / Daisy Y.M. Ng / Gigi Y.Z. Liu / Carrie K.C. Wan / Man-Chun Cheung / Ka-Chun Ng / John M. Nicholls / Dominic N.C. Tsang / Malik Peiris / Michael C.W. Chan / Leo L.M. Poon

    Emerging Infectious Diseases, Vol 27, Iss 5, Pp 1492-

    2021  Volume 1495

    Abstract: We describe an introduction of clade GH severe acute respiratory syndrome coronavirus 2 causing a fourth wave of coronavirus disease in Hong Kong. The virus has an ORF3a-Q57H mutation, causing truncation of ORF3b. This virus evades induction of cytokine, ...

    Abstract We describe an introduction of clade GH severe acute respiratory syndrome coronavirus 2 causing a fourth wave of coronavirus disease in Hong Kong. The virus has an ORF3a-Q57H mutation, causing truncation of ORF3b. This virus evades induction of cytokine, chemokine, and interferon-stimulated gene expression in primary human respiratory cells.
    Keywords respiratory infections ; severe acute respiratory syndrome coronavirus 2 ; SARS-CoV-2 ; SARS ; COVID-19 ; coronavirus disease ; Medicine ; R ; Infectious and parasitic diseases ; RC109-216
    Language English
    Publishing date 2021-05-01T00:00:00Z
    Publisher Centers for Disease Control and Prevention
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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