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  1. Article ; Online: Proteome profiling of Paenibacillus sonchi genomovar Riograndensis SBR5

    Ribeiro, Igor Daniel Alves / Paes, Jéssica Andrade / Wendisch, Volker F / Ferreira, Henrique Bunselmeyer / Passaglia, Luciane Maria Pereira

    Journal of proteomics

    2023  Volume 294, Page(s) 105061

    Abstract: Paenibacillus sonchi ... ...

    Abstract Paenibacillus sonchi SBR5
    MeSH term(s) Nitrogen Fixation ; Proteome/metabolism ; Proteomics ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Nitrogenase/metabolism ; Paenibacillus/genetics ; Paenibacillus/metabolism ; Molybdenum/metabolism ; Iron/metabolism ; Nitrogen/metabolism
    Chemical Substances Proteome ; Nitrogenase (EC 1.18.6.1) ; Molybdenum (81AH48963U) ; Iron (E1UOL152H7) ; Nitrogen (N762921K75)
    Language English
    Publishing date 2023-12-26
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2400835-7
    ISSN 1876-7737 ; 1874-3919
    ISSN (online) 1876-7737
    ISSN 1874-3919
    DOI 10.1016/j.jprot.2023.105061
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  2. Article: Genomic and Transcriptomic Investigation of the Physiological Response of the Methylotroph

    Haupka, Carsten / Brito, Luciana F / Busche, Tobias / Wibberg, Daniel / Wendisch, Volker F

    Frontiers in microbiology

    2021  Volume 12, Page(s) 664598

    Abstract: The methylotrophic ... ...

    Abstract The methylotrophic thermophile
    Language English
    Publishing date 2021-04-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2021.664598
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  3. Article ; Online: A genome-reduced Corynebacterium glutamicum derivative discloses a hidden pathway relevant for 1,2-propanediol production.

    Siebert, Daniel / Glawischnig, Erich / Wirth, Marie-Theres / Vannahme, Mieke / Salazar-Quirós, Álvaro / Weiske, Annette / Saydam, Ezgi / Möggenried, Dominik / Wendisch, Volker F / Blombach, Bastian

    Microbial cell factories

    2024  Volume 23, Issue 1, Page(s) 62

    Abstract: Background: 1,2-propanediol (1,2-PDO) is widely used in the cosmetic, food, and drug industries with a worldwide consumption of over 1.5 million metric tons per year. Although efforts have been made to engineer microbial hosts such as Corynebacterium ... ...

    Abstract Background: 1,2-propanediol (1,2-PDO) is widely used in the cosmetic, food, and drug industries with a worldwide consumption of over 1.5 million metric tons per year. Although efforts have been made to engineer microbial hosts such as Corynebacterium glutamicum to produce 1,2-PDO from renewable resources, the performance of such strains is still improvable to be competitive with existing petrochemical production routes.
    Results: In this study, we enabled 1,2-PDO production in the genome-reduced strain C. glutamicum PC2 by introducing previously described modifications. The resulting strain showed reduced product formation but secreted 50 ± 1 mM D-lactate as byproduct. C. glutamicum PC2 lacks the D-lactate dehydrogenase which pointed to a yet unknown pathway relevant for 1,2-PDO production. Further analysis indicated that in C. glutamicum methylglyoxal, the precursor for 1,2-PDO synthesis, is detoxified with the antioxidant native mycothiol (MSH) by a glyoxalase-like system to lactoylmycothiol and converted to D-lactate which is rerouted into the central carbon metabolism at the level of pyruvate. Metabolomics of cell extracts of the empty vector-carrying wildtype, a 1,2-PDO producer and its derivative with inactive D-lactate dehydrogenase identified major mass peaks characteristic for lactoylmycothiol and its precursors MSH and glucosaminyl-myo-inositol, whereas the respective mass peaks were absent in a production strain with inactivated MSH synthesis. Deletion of mshA, encoding MSH synthase, in the 1,2-PDO producing strain C. glutamicum ΔhdpAΔldh(pEKEx3-mgsA-yqhD-gldA) improved the product yield by 56% to 0.53 ± 0.01 mM
    Conclusions: Genome reduced-strains are a useful basis to unravel metabolic constraints for strain engineering and disclosed in this study the pathway to detoxify methylglyoxal which represents a precursor for 1,2-PDO production. Subsequent inactivation of the competing pathway significantly improved the 1,2-PDO yield.
    MeSH term(s) Propylene Glycol/metabolism ; Corynebacterium glutamicum/genetics ; Corynebacterium glutamicum/metabolism ; Pyruvaldehyde/metabolism ; Lactates/metabolism ; Metabolic Engineering ; Propylene Glycols
    Chemical Substances Propylene Glycol (6DC9Q167V3) ; Pyruvaldehyde (722KLD7415) ; 1,3-propanediol (5965N8W85T) ; Lactates ; Propylene Glycols
    Language English
    Publishing date 2024-02-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 2091377-1
    ISSN 1475-2859 ; 1475-2859
    ISSN (online) 1475-2859
    ISSN 1475-2859
    DOI 10.1186/s12934-024-02337-w
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  4. Article ; Online: Can Genome Sequencing Coupled to Flux Balance Analyses Offer Precision Guidance for Industrial Strain Development? The Lessons from Carbon Trafficking in

    Kurpejović, Eldin / Wibberg, Daniel / Bastem, Gülsüm Merve / Burgardt, Arthur / Busche, Tobias / Kaya, Fatma Ece Altinisik / Dräger, Andreas / Wendisch, Volker F / Akbulut, Berna Sariyar

    Omics : a journal of integrative biology

    2023  Volume 27, Issue 9, Page(s) 434–443

    Abstract: Systems biology tools offer new prospects for industrial strain selection. For bacteria that are significant for industrial applications, whole-genome sequencing coupled to flux balance analysis (FBA) can help unpack the complex relationships between ... ...

    Abstract Systems biology tools offer new prospects for industrial strain selection. For bacteria that are significant for industrial applications, whole-genome sequencing coupled to flux balance analysis (FBA) can help unpack the complex relationships between genome mutations and carbon trafficking. This work investigates the l-tyrosine (l-Tyr) overproducing model system
    MeSH term(s) Corynebacterium glutamicum/genetics ; Chromosome Mapping ; Industry ; Carbon
    Chemical Substances shikimate ; Carbon (7440-44-0)
    Language English
    Publishing date 2023-10-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2030312-9
    ISSN 1557-8100 ; 1536-2310
    ISSN (online) 1557-8100
    ISSN 1536-2310
    DOI 10.1089/omi.2023.0098
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  5. Article: Metabolic pathway engineering for production of 1,2-propanediol and 1-propanol by Corynebacterium glutamicum.

    Siebert, Daniel / Wendisch, Volker F

    Biotechnology for biofuels

    2015  Volume 8, Page(s) 91

    Abstract: Background: Production of the versatile bulk chemical 1,2-propanediol and the potential biofuel 1-propanol is still dependent on petroleum, but some approaches to establish bio-based production from renewable feed stocks and to avoid toxic intermediates ...

    Abstract Background: Production of the versatile bulk chemical 1,2-propanediol and the potential biofuel 1-propanol is still dependent on petroleum, but some approaches to establish bio-based production from renewable feed stocks and to avoid toxic intermediates have been described. The biotechnological workhorse Corynebacterium glutamicum has also been shown to be able to overproduce 1,2-propanediol by metabolic engineering. Additionally, C. glutamicum has previously been engineered for production of the biofuels ethanol and isobutanol but not for 1-propanol.
    Results: In this study, the improved production of 1,2-propanediol by C. glutamicum is presented. The product yield of a C. glutamicum strain expressing the heterologous genes gldA and mgsA from Escherichia coli that encode methylglyoxal synthase gene and glycerol dehydrogenase, respectively, was improved by additional expression of alcohol dehydrogenase gene yqhD from E. coli leading to a yield of 0.131 mol/mol glucose. Deletion of the endogenous genes hdpA and ldh encoding dihydroxyacetone phosphate phosphatase and lactate dehydrogenase, respectively, prevented formation of glycerol and lactate as by-products and improved the yield to 0.343 mol/mol glucose. To construct a 1-propanol producer, the operon ppdABC from Klebsiella oxytoca encoding diol dehydratase was expressed in the improved 1,2-propanediol producing strain ending up with 12 mM 1-propanol and up to 60 mM unconverted 1,2-propanediol. Thus, B12-dependent diol dehydratase activity may be limiting 1-propanol production.
    Conclusions: Production of 1,2-propanediol by C. glutamicum was improved by metabolic engineering targeting endogenous enzymes. Furthermore, to the best of our knowledge, production of 1-propanol by recombinant C. glutamicum was demonstrated for the first time.
    Language English
    Publishing date 2015-06-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 2421351-2
    ISSN 1754-6834
    ISSN 1754-6834
    DOI 10.1186/s13068-015-0269-0
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  6. Article ; Online: Metabolic engineering of Corynebacterium glutamicum for fatty alcohol production from glucose and wheat straw hydrolysate.

    Werner, Felix / Schwardmann, Lynn S / Siebert, Daniel / Rückert-Reed, Christian / Kalinowski, Jörn / Wirth, Marie-Theres / Hofer, Katharina / Takors, Ralf / Wendisch, Volker F / Blombach, Bastian

    Biotechnology for biofuels and bioproducts

    2023  Volume 16, Issue 1, Page(s) 116

    Abstract: Background: Fatty acid-derived products such as fatty alcohols (FAL) find growing application in cosmetic products, lubricants, or biofuels. So far, FAL are primarily produced petrochemically or through chemical conversion of bio-based feedstock. ... ...

    Abstract Background: Fatty acid-derived products such as fatty alcohols (FAL) find growing application in cosmetic products, lubricants, or biofuels. So far, FAL are primarily produced petrochemically or through chemical conversion of bio-based feedstock. Besides the well-known negative environmental impact of using fossil resources, utilization of bio-based first-generation feedstock such as palm oil is known to contribute to the loss of habitat and biodiversity. Thus, the microbial production of industrially relevant chemicals such as FAL from second-generation feedstock is desirable.
    Results: To engineer Corynebacterium glutamicum for FAL production, we deregulated fatty acid biosynthesis by deleting the transcriptional regulator gene fasR, overexpressing a fatty acyl-CoA reductase (FAR) gene of Marinobacter hydrocarbonoclasticus VT8 and attenuating the native thioesterase expression by exchange of the ATG to a weaker TTG start codon. C. glutamicum ∆fasR cg2692
    Conclusion: The combination of targeted metabolic engineering and ALE enabled efficient FAL production in C. glutamicum from wheat straw hydrolysate for the first time. Therefore, this study provides useful metabolic engineering principles to tailor this bacterium for other products from this second-generation feedstock.
    Language English
    Publishing date 2023-07-18
    Publishing country England
    Document type Journal Article
    ISSN 2731-3654
    ISSN (online) 2731-3654
    DOI 10.1186/s13068-023-02367-3
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  7. Book ; Online: Employing airborne radiation and cloud microphysics observations to improve cloud representation in ICON at kilometer-scale resolution in the Arctic

    Kretzschmar, Jan / Stapf, Johannes / Klocke, Daniel / Wendisch, Manfred / Quaas, Johannes

    eISSN: 1680-7324

    2020  

    Abstract: Clouds play a potentially important role in Arctic climate change, but are poorly represented in current atmospheric models across scales. To improve the representation of Arctic clouds in models, it is necessary to compare models to observations to ... ...

    Abstract Clouds play a potentially important role in Arctic climate change, but are poorly represented in current atmospheric models across scales. To improve the representation of Arctic clouds in models, it is necessary to compare models to observations to consequently reduce this uncertainty. This study compares aircraft observations from the Arctic Cloud Observations Using Airborne Measurements during Polar Day (ACLOUD) campaign in May/June 2017 around Svalbard, Norway – to simulations using the ICON (ICOsahedral Non-hydrostatic) model in its numerical weather prediction (NWP) set-up at 1.2 km resolution. By comparing measurements of solar and terrestrial irradiances during ACLOUD flights to the respective properties in ICON, we showed that the model systematically overestimates the transmissivity of the mostly liquid clouds during the campaign. This model bias is traced back to the way cloud condensation nuclei (CCN) get activated into cloud droplets in the two-moment, bulk microphysical scheme used in this study. This process is parameterized as function of grid-scale vertical velocity in the microphysical scheme used, but in-cloud turbulence cannot sufficiently be resolved at 1.2 km horizontal resolution in Arctic clouds. By parameterizing subgrid-scale vertical motion as a function of turbulent kinetic energy, we are able to achieve a more realistic CCN activation into cloud droplets. Additionally, we showed that by scaling the presently used CCN activation profile, the hydrometeor number concentration could be modified to be in better agreement with ACLOUD observations in our revised CCN activation parameterization. This consequently results in an improved representation of cloud optical properties in our ICON simulations.
    Subject code 551 ; 520
    Language English
    Publishing date 2020-07-02
    Publishing country de
    Document type Book ; Online
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    Inter-library loan at ZB MED

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  8. Book ; Online: Employing airborne radiation and cloud microphysics observations to improve cloud representation in ICON at kilometer-scale resolution in the Arctic

    Kretzschmar, Jan / Stapf, Johannes / Klocke, Daniel / Wendisch, Manfred / Quaas, Johannes

    eISSN: 1680-7324

    2020  

    Abstract: Clouds play a potentially important role in Arctic climate change but are poorly represented in current atmospheric models across scales. To improve the representation of Arctic clouds in models, it is necessary to compare models to observations to ... ...

    Abstract Clouds play a potentially important role in Arctic climate change but are poorly represented in current atmospheric models across scales. To improve the representation of Arctic clouds in models, it is necessary to compare models to observations to consequently reduce this uncertainty. This study compares aircraft observations from the Arctic CLoud Observations Using airborne measurements during polar Day (ACLOUD) campaign around Svalbard, Norway, in May–June 2017 and simulations using the ICON (ICOsahedral Non-hydrostatic) model in its numerical weather prediction (NWP) setup at 1.2 km horizontal resolution. By comparing measurements of solar and terrestrial irradiances during ACLOUD flights to the respective properties in ICON, we showed that the model systematically overestimates the transmissivity of the mostly liquid clouds during the campaign. This model bias is traced back to the way cloud condensation nuclei (CCN) get activated into cloud droplets in the two-moment bulk microphysical scheme used in this study. This process is parameterized as a function of grid-scale vertical velocity in the microphysical scheme used, but in-cloud turbulence cannot be sufficiently resolved at 1.2 km horizontal resolution in Arctic clouds. By parameterizing subgrid-scale vertical motion as a function of turbulent kinetic energy, we are able to achieve a more realistic CCN activation into cloud droplets. Additionally, we showed that by scaling the presently used CCN activation profile, the hydrometeor number concentration could be modified to be in better agreement with ACLOUD observations in our revised CCN activation parameterization. This consequently results in an improved representation of cloud optical properties in our ICON simulations.
    Subject code 551 ; 520
    Language English
    Publishing date 2020-11-09
    Publishing country de
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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  9. Article ; Online: Monkeypox in-patients with severe anal pain.

    Pfäfflin, Frieder / Wendisch, Daniel / Scherer, Roland / Jürgens, Linda / Godzick-Njomgang, Gisèle / Tranter, Eva / Tober-Lau, Pinkus / Stegemann, Miriam Songa / Corman, Victor Max / Kurth, Florian / Schürmann, Dirk

    Infection

    2022  Volume 51, Issue 2, Page(s) 483–487

    Abstract: Berlin is amongst the cities most affected by the current monkeypox outbreak. Here, we report clinical characteristics of the first patients with confirmed monkeypox admitted to our center. We analyzed anamnestic, clinical, and laboratory data. Within a ... ...

    Abstract Berlin is amongst the cities most affected by the current monkeypox outbreak. Here, we report clinical characteristics of the first patients with confirmed monkeypox admitted to our center. We analyzed anamnestic, clinical, and laboratory data. Within a period of 2 weeks, six patients were hospitalized in our unit. All were MSM and had practiced condomless receptive anal intercourse in the weeks preceding admission. The chief complaint in all patients but one was severe anal pain unprecedented in severity. Investigations revealed proctitis, as well as anal and rectal ulcers with detection of monkeypox virus. Our findings support the hypothesis that sexual transmission plays a role in the current outbreak.
    MeSH term(s) Male ; Humans ; Homosexuality, Male ; HIV Infections/epidemiology ; Mpox (monkeypox) ; Sexual Behavior ; Pain
    Language English
    Publishing date 2022-08-12
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 185104-4
    ISSN 1439-0973 ; 0300-8126 ; 0173-2129
    ISSN (online) 1439-0973
    ISSN 0300-8126 ; 0173-2129
    DOI 10.1007/s15010-022-01896-7
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    In stock of ZB MED Cologne/Königswinter

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  10. Article: Evolving a New Efficient Mode of Fructose Utilization for Improved Bioproduction in

    Krahn, Irene / Bonder, Daniel / Torregrosa-Barragán, Lucía / Stoppel, Dominik / Krause, Jens P / Rosenfeldt, Natalie / Meiswinkel, Tobias M / Seibold, Gerd M / Wendisch, Volker F / Lindner, Steffen N

    Frontiers in bioengineering and biotechnology

    2021  Volume 9, Page(s) 669093

    Abstract: Fructose utilization ... ...

    Abstract Fructose utilization in
    Language English
    Publishing date 2021-05-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2021.669093
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