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Article ; Online: BSL2-compliant lethal mouse model of SARS-CoV-2 and variants of concern to evaluate therapeutics targeting the Spike protein.

Manangeeswaran, Mohanraj / Ireland, Derek D C / Thacker, Seth G / Lee, Ha-Na / Kelley-Baker, Logan / Lewkowicz, Aaron P / Rothlauf, Paul W / Cornejo Pontelli, Marjorie / Bloyet, Louis-Marie / Eckhaus, Michael A / Mendoza, Mirian I / Whelan, Sean / Verthelyi, Daniela

Frontiers in immunology

2022 Volume 13, Page(s) 919815

Abstract: Since first reported in 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is rapidly acquiring mutations, particularly in the spike protein, that can modulate pathogenicity, transmission and antibody evasion leading to successive waves ... ...

Abstract	Since first reported in 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is rapidly acquiring mutations, particularly in the spike protein, that can modulate pathogenicity, transmission and antibody evasion leading to successive waves of COVID19 infections despite an unprecedented mass vaccination necessitating continuous adaptation of therapeutics. Small animal models can facilitate understanding host-pathogen interactions, target selection for therapeutic drugs, and vaccine development, but availability and cost of studies in BSL3 facilities hinder progress. To generate a BSL2-compatible
MeSH term(s)	Animals ; COVID-19 ; Disease Models, Animal ; Humans ; Membrane Glycoproteins/metabolism ; Mice ; Receptors, Immunologic ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus/genetics
Chemical Substances	LILRB4 protein, human ; Membrane Glycoproteins ; Receptors, Immunologic ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
Language	English
Publishing date	2022-07-28
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.919815
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Article: Neural Infection by Oropouche Virus in Adult Human Brain Slices Induces an Inflammatory and Toxic Response.

Almeida, Glaucia M / Souza, Juliano P / Mendes, Niele D / Pontelli, Marjorie C / Pinheiro, Nathalia R / Nogueira, Giovanna O / Cardoso, Ricardo S / Paiva, Isadora M / Ferrari, Gustavo D / Veras, Flávio P / Cunha, Fernando Q / Horta-Junior, Jose A C / Alberici, Luciane C / Cunha, Thiago M / Podolsky-Gondim, Guilherme G / Neder, Luciano / Arruda, Eurico / Sebollela, Adriano

Frontiers in neuroscience

2021 Volume 15, Page(s) 674576

Abstract: Oropouche virus (OROV) is an emerging arbovirus in South and Central Americas with high spreading potential. OROV infection has been associated with neurological complications and OROV genomic RNA has been detected in cerebrospinal fluid from patients, ... ...

Abstract	Oropouche virus (OROV) is an emerging arbovirus in South and Central Americas with high spreading potential. OROV infection has been associated with neurological complications and OROV genomic RNA has been detected in cerebrospinal fluid from patients, suggesting its neuroinvasive potential. Motivated by these findings, neurotropism and neuropathogenesis of OROV have been investigated
Language	English
Publishing date	2021-11-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2411902-7
ISSN	1662-453X ; 1662-4548
ISSN (online)	1662-453X
ISSN	1662-4548
DOI	10.3389/fnins.2021.674576
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Article ; Online: Silent Infection of B and CD8

Castro, Italo A / Jorge, Daniel M M / Ferreri, Lucas M / Martins, Ronaldo B / Pontelli, Marjorie C / Jesus, Bruna L S / Cardoso, Ricardo S / Criado, Miria F / Carenzi, Lucas / Valera, Fabiana C P / Tamashiro, Edwin / Anselmo-Lima, Wilma T / Perez, Daniel R / Arruda, Eurico

Journal of virology

2020 Volume 94, Issue 9

Abstract: Influenza A viruses (IAVs) cause more than 2 million annual episodes of seasonal acute respiratory infections (ARI) and approximately 500,000 deaths worldwide. Depending on virus strain and host immune status, acute infections by IAV may reach sites ... ...

Abstract	Influenza A viruses (IAVs) cause more than 2 million annual episodes of seasonal acute respiratory infections (ARI) and approximately 500,000 deaths worldwide. Depending on virus strain and host immune status, acute infections by IAV may reach sites other than the respiratory tract. In the present study, IAV RNA and antigens were searched for in tissues of palatine tonsils and adenoids removed from patients without ARI symptoms. A real-time reverse transcriptase PCR (RT-PCR) screening revealed that 8 tissue samples from 7 patients out of 103 were positive for IAV. Positive samples were subjected to next-generation sequencing (NGS) and 3 of 8 tissues yielded complete IAV pH1N1 genomes, whereas in 5 samples, the PB1 gene was not fully assembled. Phylogenetic analysis placed tonsil-derived IAV in clusters clearly segregated from contemporaneous Brazilian viruses. Flow cytometry of dispersed tissue fragments and serial immunohistochemistry of paraffin-embedded sections of naturally infected biopsies indicated that CD20
MeSH term(s)	Adenoids/pathology ; Adolescent ; Animals ; B-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/immunology ; Child ; Child, Preschool ; Cross-Sectional Studies ; Dogs ; Female ; Humans ; Hypertrophy ; Influenza A virus/immunology ; Influenza, Human/immunology ; Influenza, Human/virology ; Madin Darby Canine Kidney Cells ; Male ; Palatine Tonsil/pathology ; Phylogeny ; Prospective Studies ; T-Lymphocytes/pathology ; Tonsillectomy/methods ; Tonsillitis/complications ; Tonsillitis/surgery ; Tonsillitis/virology ; Virus Replication ; Virus Shedding
Language	English
Publishing date	2020-04-16
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80174-4
ISSN	1098-5514 ; 0022-538X
ISSN (online)	1098-5514
ISSN	0022-538X
DOI	10.1128/JVI.01969-19
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Article ; Online: High-Dose Convalescent Plasma for Treatment of Severe COVID-19.

De Santis, Gil C / Oliveira, Luciana Correa / Garibaldi, Pedro M M / Almado, Carlos E L / Croda, Julio / Arcanjo, Ghislaine G A / Oliveira, Érika A F / Tonacio, Adriana C / Langhi, Dante M / Bordin, José O / Gilio, Renato N / Palma, Leonardo C / Santos, Elaine V / Haddad, Simone K / Prado, Benedito P A / Pontelli, Marjorie Cornejo / Gomes, Rogério / Miranda, Carlos H / Auxiliadora Martins, Maria /

Covas, Dimas T / Arruda, Eurico / Fonseca, Benedito A L / Calado, Rodrigo T

Emerging infectious diseases

2022 Volume 28, Issue 3, Page(s) 548–555

Abstract: To assess whether high-dose coronavirus disease (COVID-19) convalescent plasma (CCP) transfusion may benefit patients with severe COVID-19, we conducted a multicenter randomized trial in Brazil. Patients with severe COVID-19 who were within 10 days of ... ...

Abstract	To assess whether high-dose coronavirus disease (COVID-19) convalescent plasma (CCP) transfusion may benefit patients with severe COVID-19, we conducted a multicenter randomized trial in Brazil. Patients with severe COVID-19 who were within 10 days of initial symptom onset were eligible. Patients in the CCP group received 3 daily doses of CCP (600 mL/d) in addition to standard treatment; control patients received standard treatment only. Primary outcomes were death rates at days 30 and 60 of study randomization. Secondary outcomes were ventilator-free days and hospital-free days. We enrolled 107 patients: 36 CCP and 71 control. At day 30, death rates were 22% for CCP and 25% for the control group; at day 60, rates were 31% for CCP and 35% for control. Needs for invasive mechanical ventilation and durations of hospital stay were similar between groups. We conclude that high-dose CCP transfused within 10 days of symptom onset provided no benefit for patients with severe COVID-19.
MeSH term(s)	COVID-19/therapy ; Humans ; Immunization, Passive/adverse effects ; Plasma ; SARS-CoV-2 ; Treatment Outcome
Language	English
Publishing date	2022-01-26
Publishing country	United States
Document type	Journal Article ; Multicenter Study ; Randomized Controlled Trial
ZDB-ID	1380686-5
ISSN	1080-6059 ; 1080-6040
ISSN (online)	1080-6059
ISSN	1080-6040
DOI	10.3201/eid2803.212299
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Article ; Online: A class II MHC-targeted vaccine elicits immunity against SARS-CoV-2 and its variants.

Proceedings of the National Academy of Sciences of the United States of America

2021 Volume 118, Issue 44

Abstract: The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in over 100 million infections and millions of deaths. Effective vaccines remain the best hope of curtailing SARS-CoV-2 transmission, morbidity, and ... ...

Abstract	The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in over 100 million infections and millions of deaths. Effective vaccines remain the best hope of curtailing SARS-CoV-2 transmission, morbidity, and mortality. The vaccines in current use require cold storage and sophisticated manufacturing capacity, which complicates their distribution, especially in less developed countries. We report the development of a candidate SARS-CoV-2 vaccine that is purely protein based and directly targets antigen-presenting cells. It consists of the SARS-CoV-2 Spike receptor-binding domain (Spike
MeSH term(s)	Amino Acid Sequence ; Animals ; Antibodies, Neutralizing/biosynthesis ; Antibodies, Viral/biosynthesis ; Antigen-Presenting Cells/immunology ; CD8-Positive T-Lymphocytes/immunology ; COVID-19/epidemiology ; COVID-19/immunology ; COVID-19/prevention & control ; COVID-19 Vaccines/administration & dosage ; COVID-19 Vaccines/immunology ; COVID-19 Vaccines/pharmacology ; Camelids, New World/immunology ; Female ; Histocompatibility Antigens Class II/immunology ; Humans ; Immunity, Cellular ; Immunity, Humoral ; Immunization, Secondary ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Transgenic ; Pandemics/prevention & control ; Recombinant Fusion Proteins/administration & dosage ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/immunology ; SARS-CoV-2/genetics ; SARS-CoV-2/immunology ; Single-Domain Antibodies/administration & dosage ; Single-Domain Antibodies/immunology ; Spike Glycoprotein, Coronavirus/administration & dosage ; Spike Glycoprotein, Coronavirus/genetics ; Spike Glycoprotein, Coronavirus/immunology
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 Vaccines ; Histocompatibility Antigens Class II ; Recombinant Fusion Proteins ; Single-Domain Antibodies ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
Language	English
Publishing date	2021-03-24
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.2116147118
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Article ; Online: High-Dose Convalescent Plasma for Treatment of Severe COVID-19

Gil C. De Santis / Luciana Correa Oliveira / Pedro M.M. Garibaldi / Carlos E.L. Almado / Julio Croda / Ghislaine G.A. Arcanjo / Érika A.F. Oliveira / Adriana C. Tonacio / Dante M. Langhi / José O. Bordin / Renato N. Gilio / Leonardo C. Palma / Elaine V. Santos / Simone K. Haddad / Benedito P.A. Prado / Marjorie Cornejo Pontelli / Rogério Gomes / Carlos H. Miranda / Maria Auxiliadora Martins /

Dimas T. Covas / Eurico Arruda / Benedito A.L. Fonseca / Rodrigo T. Calado

Emerging Infectious Diseases, Vol 28, Iss 3, Pp 548-

2022 Volume 555

Abstract	To assess whether high-dose coronavirus disease (COVID-19) convalescent plasma (CCP) transfusion may benefit patients with severe COVID-19, we conducted a multicenter randomized trial in Brazil. Patients with severe COVID-19 who were within 10 days of initial symptom onset were eligible. Patients in the CCP group received 3 daily doses of CCP (600 mL/d) in addition to standard treatment; control patients received standard treatment only. Primary outcomes were death rates at days 30 and 60 of study randomization. Secondary outcomes were ventilator-free days and hospital-free days. We enrolled 107 patients: 36 CCP and 71 control. At day 30, death rates were 22% for CCP and 25% for the control group; at day 60, rates were 31% for CCP and 35% for control. Needs for invasive mechanical ventilation and durations of hospital stay were similar between groups. We conclude that high-dose CCP transfused within 10 days of symptom onset provided no benefit for patients with severe COVID-19.
Keywords	COVID-19 ; 2019 novel coronavirus disease ; coronavirus disease ; severe acute respiratory syndrome coronavirus 2 ; SARS-CoV-2 ; viruses ; Medicine ; R ; Infectious and parasitic diseases ; RC109-216
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	Centers for Disease Control and Prevention
Document type	Article ; Online
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Article ; Online: Vesicular Stomatitis Virus Chimeras Expressing the Oropouche Virus Glycoproteins Elicit Protective Immune Responses in Mice.

Stubbs, Sarah Hulsey / Cornejo Pontelli, Marjorie / Mishra, Nischay / Zhou, Changhong / de Paula Souza, Juliano / Mendes Viana, Rosa Maria / Lipkin, W Ian / Knipe, David M / Arruda, Eurico / Whelan, Sean P J

mBio

2021 Volume 12, Issue 4, Page(s) e0046321

Abstract: Oropouche virus (OROV) infection of humans is associated with a debilitating febrile illness that can progress to meningitis or encephalitis. First isolated from a forest worker in Trinidad and Tobago in 1955, the arbovirus OROV has since been detected ... ...

Abstract	Oropouche virus (OROV) infection of humans is associated with a debilitating febrile illness that can progress to meningitis or encephalitis. First isolated from a forest worker in Trinidad and Tobago in 1955, the arbovirus OROV has since been detected throughout the Amazon basin with an estimated 500,000 human infections over 60 years. Like other members of the family
MeSH term(s)	Animals ; Antibodies, Neutralizing ; Bunyaviridae Infections/immunology ; Bunyaviridae Infections/prevention & control ; Genome, Viral ; Male ; Mice ; Mice, Inbred C57BL ; Orthobunyavirus/genetics ; Vesicular Stomatitis/virology ; Vesiculovirus/genetics ; Vesiculovirus/immunology ; Viral Envelope Proteins/genetics ; Virus Replication
Chemical Substances	Antibodies, Neutralizing ; Viral Envelope Proteins
Language	English
Publishing date	2021-08-03
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mBio.00463-21
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Article ; Online: SARS-CoV-2 productively infects primary human immune system cells in vitro and in COVID-19 patients.

Pontelli, Marjorie C / Castro, Ítalo A / Martins, Ronaldo B / La Serra, Leonardo / Veras, Flávio P / Nascimento, Daniele C / Silva, Camila M / Cardoso, Ricardo S / Rosales, Roberta / Gomes, Rogério / Lima, Thais M / Souza, Juliano P / Vitti, Brenda C / Caetité, Diego B / de Lima, Mikhael H F / Stumpf, Spencer D / Thompson, Cassandra E / Bloyet, Louis-Marie / Toller-Kawahisa, Juliana E /

Giannini, Marcela C / Bonjorno, Letícia P / Lopes, Maria I F / Batah, Sabrina S / Siyuan, Li / Luppino-Assad, Rodrigo / Almeida, Sergio C L / Oliveira, Fabiola R / Benatti, Maíra N / Pontes, Lorena L F / Santana, Rodrigo C / Vilar, Fernando C / Auxiliadora-Martins, Maria / Shi, Pei-Yong / Cunha, Thiago M / Calado, Rodrigo T / Alves-Filho, José C / Zamboni, Dario S / Fabro, Alexandre T / Louzada-Junior, Paulo / Oliveira, Rene D R / Whelan, Sean P J / Cunha, Fernando Q / Arruda, Eurico

Journal of molecular cell biology

2022 Volume 14, Issue 4

Abstract: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with a hyperinflammatory state and lymphocytopenia, a hallmark that appears as both signature and prognosis of disease severity outcome. Although cytokine storm and ... ...

Abstract	The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with a hyperinflammatory state and lymphocytopenia, a hallmark that appears as both signature and prognosis of disease severity outcome. Although cytokine storm and a sustained inflammatory state are commonly associated with immune cell depletion, it is still unclear whether direct SARS-CoV-2 infection of immune cells could also play a role in this scenario by harboring viral replication. We found that monocytes, as well as both B and T lymphocytes, were susceptible to SARS-CoV-2 infection in vitro, accumulating double-stranded RNA consistent with viral RNA replication and ultimately leading to expressive T cell apoptosis. In addition, flow cytometry and immunofluorescence analysis revealed that SARS-CoV-2 was frequently detected in monocytes and B lymphocytes from coronavirus disease 2019 (COVID-19) patients. The rates of SARS-CoV-2-infected monocytes in peripheral blood mononuclear cells from COVID-19 patients increased over time from symptom onset, with SARS-CoV-2-positive monocytes, B cells, and CD4+ T lymphocytes also detected in postmortem lung tissue. These results indicated that SARS-CoV-2 infection of blood-circulating leukocytes in COVID-19 patients might have important implications for disease pathogenesis and progression, immune dysfunction, and virus spread within the host.
MeSH term(s)	COVID-19 ; Cytokine Release Syndrome ; Humans ; Leukocytes, Mononuclear ; Monocytes ; SARS-CoV-2
Language	English
Publishing date	2022-04-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2500949-7
ISSN	1759-4685 ; 1759-4685
ISSN (online)	1759-4685
ISSN	1759-4685
DOI	10.1093/jmcb/mjac021
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Article ; Online: Mitochondrial DNA and TLR9 activation contribute to SARS-CoV-2-induced endothelial cell damage.

Costa, Tiago J / Potje, Simone R / Fraga-Silva, Thais F C / da Silva-Neto, Júlio A / Barros, Paula R / Rodrigues, Daniel / Machado, Mirele R / Martins, Ronaldo B / Santos-Eichler, Rosangela A / Benatti, Maira N / de Sá, Keyla S G / Almado, Carlos Eduardo L / Castro, Ítalo A / Pontelli, Marjorie C / Serra, Leonardo La / Carneiro, Fernando S / Becari, Christiane / Louzada-Junior, Paulo / Oliveira, Rene D R /

Zamboni, Dario S / Arruda, Eurico / Auxiliadora-Martins, Maria / Giachini, Fernanda R C / Bonato, Vânia L D / Zachara, Natasha E / Bomfim, Gisele F / Tostes, Rita C

Vascular pharmacology

2021 Volume 142, Page(s) 106946

Abstract: Background and purpose: Mitochondria play a central role in the host response to viral infection and immunity, being key to antiviral signaling and exacerbating inflammatory processes. Mitochondria and Toll-like receptor (TLR) have been suggested as ... ...

Abstract	Background and purpose: Mitochondria play a central role in the host response to viral infection and immunity, being key to antiviral signaling and exacerbating inflammatory processes. Mitochondria and Toll-like receptor (TLR) have been suggested as potential targets in SARS-CoV-2 infection. However, the involvement of TLR9 in SARS-Cov-2-induced endothelial dysfunction and potential contribution to cardiovascular complications in COVID-19 have not been demonstrated. This study determined whether infection of endothelial cells by SARS-CoV-2 affects mitochondrial function and induces mitochondrial DNA (mtDNA) release. We also questioned whether TLR9 signaling mediates the inflammatory responses induced by SARS-CoV-2 in endothelial cells. Experimental approach: Human umbilical vein endothelial cells (HUVECs) were infected by SARS-CoV-2 and immunofluorescence was used to confirm the infection. Mitochondrial function was analyzed by specific probes and mtDNA levels by real-time polymerase chain reaction (RT-PCR). Inflammatory markers were measured by ELISA, protein expression by western blot, intracellular calcium (Ca Key results: SARS-CoV-2 infected HUVECs, which express ACE2 and TMPRSS2 proteins, and promoted mitochondrial dysfunction, i.e. it increased mitochondria-derived superoxide anion, mitochondrial membrane potential, and mtDNA release, leading to activation of TLR9 and NF-kB, and release of cytokines. SARS-CoV-2 also decreased nitric oxide synthase (eNOS) expression and inhibited Ca Conclusion and applications: SARS-CoV-2 infection impairs mitochondrial function and activates TLR9 signaling in endothelial cells. TLR9 triggers inflammatory responses that lead to endothelial cell dysfunction, potentially contributing to the severity of symptoms in COVID-19. Targeting mitochondrial metabolic pathways may help to define novel therapeutic strategies for COVID-19.
MeSH term(s)	Animals ; COVID-19 ; DNA, Mitochondrial/genetics ; DNA, Mitochondrial/metabolism ; Endothelial Cells/metabolism ; Humans ; Mice ; Mitochondria/metabolism ; SARS-CoV-2 ; Toll-Like Receptor 9/genetics ; Toll-Like Receptor 9/metabolism
Chemical Substances	DNA, Mitochondrial ; TLR9 protein, human ; Tlr9 protein, mouse ; Toll-Like Receptor 9
Language	English
Publishing date	2021-11-25
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2082846-9
ISSN	1879-3649 ; 1537-1891 ; 1879-3649
ISSN (online)	1879-3649 ; 1537-1891
ISSN	1879-3649
DOI	10.1016/j.vph.2021.106946
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Article ; Online: ESCRT machinery components are required for Orthobunyavirus particle production in Golgi compartments.

Barbosa, Natalia S / Mendonça, Leila R / Dias, Marcos V S / Pontelli, Marjorie C / da Silva, Elaine Z M / Criado, Miria F / da Silva-Januário, Mara E / Schindler, Michael / Jamur, Maria C / Oliver, Constance / Arruda, Eurico / daSilva, Luis L P

PLoS pathogens

2018 Volume 14, Issue 5, Page(s) e1007047

Abstract: Peribunyaviridae is a large family of RNA viruses with several members that cause mild to severe diseases in humans and livestock. Despite their importance in public heath very little is known about the host cell factors hijacked by these viruses to ... ...

Abstract	Peribunyaviridae is a large family of RNA viruses with several members that cause mild to severe diseases in humans and livestock. Despite their importance in public heath very little is known about the host cell factors hijacked by these viruses to support assembly and cell egress. Here we show that assembly of Oropouche virus, a member of the genus Orthobunyavirus that causes a frequent arboviral infection in South America countries, involves budding of virus particles toward the lumen of Golgi cisternae. As viral replication progresses, these Golgi subcompartments become enlarged and physically separated from Golgi stacks, forming Oropouche viral factory (Vfs) units. At the ultrastructural level, these virally modified Golgi cisternae acquire an MVB appearance, and while they lack typical early and late endosome markers, they become enriched in endosomal complex required for transport (ESCRT) proteins that are involved in MVB biogenesis. Further microscopy and viral replication analysis showed that functional ESCRT machinery is required for efficient Vf morphogenesis and production of infectious OROV particles. Taken together, our results indicate that OROV attracts ESCRT machinery components to Golgi cisternae to mediate membrane remodeling events required for viral assembly and budding at these compartments. This represents an unprecedented mechanism of how viruses hijack host cell components for coordinated morphogenesis.
MeSH term(s)	Cell Culture Techniques ; Endosomal Sorting Complexes Required for Transport/metabolism ; Endosomal Sorting Complexes Required for Transport/physiology ; Endosomes/metabolism ; Golgi Apparatus/metabolism ; Golgi Apparatus/virology ; HeLa Cells ; Humans ; Orthobunyavirus/growth & development ; Orthobunyavirus/metabolism ; Orthobunyavirus/pathogenicity ; Orthobunyavirus/physiology ; Virion/metabolism ; Virus Assembly/physiology ; Virus Release/physiology ; Virus Replication/physiology
Chemical Substances	Endosomal Sorting Complexes Required for Transport
Language	English
Publishing date	2018
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7366
ISSN (online)	1553-7374
ISSN	1553-7366
DOI	10.1371/journal.ppat.1007047
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