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Article ; Online: Establishment and application of a rapid molecular diagnostic platform for the isothermal visual amplification of group B Streptococcus based on recombinase polymerase.

Liu, Meilin / Wang, Huan / Chu, Chu / Min, Fanli / Sun, Lizhou / Zhang, Teng / Meng, Qian

Frontiers in cellular and infection microbiology

2024 Volume 14, Page(s) 1281827

Abstract: With growing concerns about Group B streptococcal (GBS) infections and their adverse effects on perinatal pregnancies, including infection, premature delivery, neonatal septicemia, and meningitis, it is urgent to promote GBS screening at all pregnancy ... ...

Abstract	With growing concerns about Group B streptococcal (GBS) infections and their adverse effects on perinatal pregnancies, including infection, premature delivery, neonatal septicemia, and meningitis, it is urgent to promote GBS screening at all pregnancy stages. The purpose of this study is to establish a device-independent, fast, sensitive, and visual GBS detection method. Taking advantage of the characteristics of the recombinase polymerase isothermal amplification (RPA), the activity of the nfo nuclease cleavage base analog (tetrahydrofuran, THF) site, and the advantages of visual reading of the lateral flow chromatography strip (LFS), a GBS detection method was developed. This method focused on the conservative region of the Christie-Atkins-Munch-Petersen factor encoded by the cfb gene, a virulence gene specific to GBS. Two forward primers, two biotin-labeled reverse primers, and one fluorescein isothiocyanate (FITC)-labeled and C3spacer-blocked probe were designed. The study involved optimizing the primer pair and probe combination, determining the optimal reaction temperature and time, evaluating specificity, analyzing detection limits, and testing the method on 87 vaginal swabs from perinatal pregnant women. The results showed that the visual detection method of GBS-RPA-LFS, using the cfb-F1/R2/P1 primer probe, could detect GBS within 15 min at the temperature ranging from 39°C to 42°C. Furthermore, the method specifically amplified only GBS, without cross-reacting with pathogens like
MeSH term(s)	Infant, Newborn ; Female ; Pregnancy ; Humans ; Recombinases ; Nucleic Acid Amplification Techniques/methods ; Sensitivity and Specificity ; Pathology, Molecular ; Nucleotidyltransferases ; Streptococcus agalactiae/genetics ; Streptococcal Infections/diagnosis
Chemical Substances	Recombinases ; Nucleotidyltransferases (EC 2.7.7.-)
Language	English
Publishing date	2024-02-23
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2619676-1
ISSN	2235-2988 ; 2235-2988
ISSN (online)	2235-2988
ISSN	2235-2988
DOI	10.3389/fcimb.2024.1281827
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Article ; Online: DNA-Peptide Interaction-Modulated Charge Reversal in Biomimetic Nanochannels for Simple and Efficient Detection of Histone Deacetylases.

Shi, Liu / Ying, Keqin / Sha, Lingjun / Zhang, Yuanyuan / Sun, Lizhou / Li, Genxi

Analytical chemistry

2024 Volume 96, Issue 12, Page(s) 4817–4824

Abstract: Protein acetylation, a fundamental post-translational modification, plays a critical role in the regulation of gene expression and cellular processes. Monitoring histone deacetylases (HDACs) is important for understanding epigenetic dynamics and ... ...

Abstract	Protein acetylation, a fundamental post-translational modification, plays a critical role in the regulation of gene expression and cellular processes. Monitoring histone deacetylases (HDACs) is important for understanding epigenetic dynamics and advancing the early diagnosis of malignancies. Here, we leverage the dynamic characteristics of DNA-peptide interactions in biomimetic nanochannels to develop a HDAC detection method. In specific, the catalysis of peptide deacetylation by HDACs triggers alterations in the charge states of the nanochannel surface to accommodate DNA molecules. Then, the interaction between DNA and peptides shifts the nanochannel surface charge from positive to negative, leading to a reversal of the ion current rectification (ICR). By calculation of the ICR ratio, quantitative detection of HDACs can be efficiently achieved using the nanochannel-based method in an enzyme-free and label-free manner. Our experimental results demonstrate that HDACs can be detected by using this method within a concentration range of 0.5-500 nM. The innate simplicity and efficiency of this strategy may render it a valuable tool for advancing both fundamental research and clinical applications in the realm of epigenetics and personalized medicine.
MeSH term(s)	Histone Deacetylases/metabolism ; Biomimetics ; DNA/metabolism ; Peptides/metabolism ; Epigenesis, Genetic ; Acetylation ; Histone Deacetylase Inhibitors
Chemical Substances	Histone Deacetylases (EC 3.5.1.98) ; DNA (9007-49-2) ; Peptides ; Histone Deacetylase Inhibitors
Language	English
Publishing date	2024-03-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c04819
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Article ; Online: Revealing the Effects of Endoplasmic Reticulum Stress on Ferroptosis by Two-Channel Real-Time Imaging of pH and Viscosity.

Song, Wenhui / Zhang, Weiyao / Yue, Lizhou / Lin, Weiying

Analytical chemistry

2022 Volume 94, Issue 17, Page(s) 6557–6565

Abstract: Endoplasmic reticulum (ER) is sensitive to changes in the intracellular environment such as pH and viscosity, and slight changes may trigger stress response. Besides, different from apoptosis and necrosis, ferroptosis is the result of lipid peroxidation ... ...

Abstract	Endoplasmic reticulum (ER) is sensitive to changes in the intracellular environment such as pH and viscosity, and slight changes may trigger stress response. Besides, different from apoptosis and necrosis, ferroptosis is the result of lipid peroxidation accumulation. There is evidence that ferroptosis is closely related to endoplasmic reticulum stress (ERS). However, the possible changes in the pH and viscosity of the ER during the ferroptosis process have not yet been studied. Therefore, we used a new type of ER-targeted dual-excitation fluorescent probe (
MeSH term(s)	Dithiothreitol/pharmacology ; Endoplasmic Reticulum Stress ; Ferroptosis ; Fluorescent Dyes/pharmacology ; Hydrogen-Ion Concentration ; Viscosity
Chemical Substances	Fluorescent Dyes ; Dithiothreitol (T8ID5YZU6Y)
Language	English
Publishing date	2022-04-18
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.2c00387
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Article ; Online: Long-term outcomes of endoscopic or surgical resection in T1 colorectal cancer patients: a retrospective cohort study.

Song, Shibo / Dou, Lizhou / Zhang, Yueming / Liu, Xudong / Liu, Yong / He, Shun / Wang, Guiqi

Surgical endoscopy

2024 Volume 38, Issue 3, Page(s) 1499–1511

Abstract: Background: The personalized treatments of T1 colorectal cancer (CRC) remains controversial. We compared the long-term outcomes of T1 CRC patients after endoscopic resection (ER) and surgery, and evaluated the risk factors for the long-term prognosis.!## ...

Abstract	Background: The personalized treatments of T1 colorectal cancer (CRC) remains controversial. We compared the long-term outcomes of T1 CRC patients after endoscopic resection (ER) and surgery, and evaluated the risk factors for the long-term prognosis. Methods: T1 CRCs after resection at the Cancer Hospital, Chines Academy of Medical Sciences from June 2011 to November 2021 were reviewed. High-risk factors included positive resection margin, poor differentiation, deep submucosal invasion (DSI ≥ 1000 μm), lymphovascular invasion and intermediate/high tumor budding. Comparative analyses were conducted based on three treatment methods: follow-up after ER (Group A), additional surgery after ER (Group B) and initial surgery (Group C). The primary endpoints included recurrence-free survival (RFS) and overall survival (OS). Cox proportional hazard regression models were constructed to identify risk factors for RFS and OS. Results: A total of 528 patients were enrolled (173 patients in Group A, 102 patients in Group B, 253 patients in Group C). The 3-year RFS, 5-year RFS, 3-year OS, and 5-year OS rates were 96.7%, 94.7%, 99.1%, and 97.8%, respectively. In the absence of other high-risk factors, RFS (P = 0.321) and OS (P = 0.155) of patients with DSI after ER were not inferior to those after surgery. Multivariate analyses identified sex (HR 0.379; 95% CI 0.160-0.894), Charlson comorbidities index (CCI) (HR 3.330; 95% CI 1.571-7.062), margin (HR 8.212; 95% CI 2.325-29.006), and budding (HR 3.794; 95% CI 1.686-8.541) as independent predictive factors of RFS, and identified CCI (HR 10.266; 95% CI 2.856-36.899) as an independent predictive factor of OS. Conclusion: The long-term outcomes of ER are comparable to those of surgery in T1 CRC patients with DSI when other high-risk factors are negative. Resection margin, tumor budding, sex, and CCI may be the most important long-term prognostic factors for T1 CRC patients.
MeSH term(s)	Humans ; Retrospective Studies ; Margins of Excision ; Colorectal Neoplasms/pathology ; Endoscopy ; Prognosis ; Neoplasm Recurrence, Local/pathology
Language	English
Publishing date	2024-01-19
Publishing country	Germany
Document type	Journal Article
ZDB-ID	639039-0
ISSN	1432-2218 ; 0930-2794
ISSN (online)	1432-2218
ISSN	0930-2794
DOI	10.1007/s00464-023-10586-w
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Article: Analysis of the Taxonomy, Synteny, and Virulence Factors for Soft Rot Pathogen

Zhang, Yanan / Chu, Honglong / Yu, Liqiong / He, Fei / Gao, Yong / Tang, Lizhou

Frontiers in microbiology

2022 Volume 13, Page(s) 868709

Abstract: Bacterial soft rot is a devastating disease for a wide range of crops, vegetables, and ornamental plants including konjac ( ...

Abstract	Bacterial soft rot is a devastating disease for a wide range of crops, vegetables, and ornamental plants including konjac (
Language	English
Publishing date	2022-07-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2022.868709
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Article ; Online: Protection of electroactive biofilms against hypersaline shock by quorum sensing.

Zhou, Shaofeng / An, Wenwen / Zhao, Kexin / Lin, Lizhou / Yang, Shan / Zhang, Yifeng / Xu, Meiying

Water research

2023 Volume 233, Page(s) 119823

Abstract: Quorum sensing (QS) is an ideal strategy for boosting the operating performance of electroactive biofilms (EABs), but its potential effects on the protection of electroactive biofilms against environmental shocks (e.g., hypersaline shock) have been ... ...

Abstract	Quorum sensing (QS) is an ideal strategy for boosting the operating performance of electroactive biofilms (EABs), but its potential effects on the protection of electroactive biofilms against environmental shocks (e.g., hypersaline shock) have been rarely revealed. In this study, a QS signaling molecule, the N-(3-oxo-dodecanoyl)-L-homoserine lactone, was employed to promote the anti-shock property of the EABs against extreme saline shock. The maximum current density of the QS-regulated biofilm recovered to 0.17 mA/cm
MeSH term(s)	Quorum Sensing ; Biofilms ; Lactones ; Bacteria ; Pseudomonas
Chemical Substances	Lactones
Language	English
Publishing date	2023-03-01
Publishing country	England
Document type	Journal Article
ZDB-ID	202613-2
ISSN	1879-2448 ; 0043-1354
ISSN (online)	1879-2448
ISSN	0043-1354
DOI	10.1016/j.watres.2023.119823
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Article ; Online: Factors associated with faster axial elongation after orthokeratology treatment.

Qi, Ya / Liu, Lizhou / Li, Yu / Zhang, Fengju

BMC ophthalmology

2022 Volume 22, Issue 1, Page(s) 62

Abstract: Background: To study the baseline factors that related to faster axial elongation after orthokeratology (OK) treatment and the characteristics of cases with axial length decrease in a group of myopia children.: Methods: This is a retrospective study. ...

Abstract	Background: To study the baseline factors that related to faster axial elongation after orthokeratology (OK) treatment and the characteristics of cases with axial length decrease in a group of myopia children. Methods: This is a retrospective study. The records of 73 children who had wear OK lens for at least one year were reviewed. Only the data of right eyes were included. Baseline data included: age, gender, parental myopia, refractive error, corneal power, central corneal thickness, axial length and anterior chamber depth. Corneal power, central corneal thickness, anterior chamber depth and axial length after one-year of OK lens wear were also collected. The related factors affecting axial length change were analyzed. A comparison was made on the cases of axial length increase and axial length decrease. Results: Of the 73 eyes, axial length increased by 0.18 ± 0.17 mm (P < 0.001) after one year of OK lens wear. Correlation analysis showed that one-year axial length change was negatively correlated with age and positively correlated with the parental myopia and baseline myopia. Stepwise multiple linear regression analysis showed that the factors associated with faster axial elongation were lower baseline myopic spherical equivalent (P = 0.018), higher parental myopia degree (P = 0.026), and younger age at the onset of lens wear. (P = 0.039). Nine eyes showed negative axial growth (-0.06 ± 0.04 mm), and had older initial age of lens wear, higher baseline myopic spherical equivalent, and lager baseline corneal power, when compared with cases of axial length increase. Conclusions: Myopia children with lower baseline myopic spherical equivalent, younger initial age and higher parental myopia had faster axial elongation after orthokeratology treatment. More aggressive treatment should be considered. In children with slow axial elongation, OK lens wear may lead to negative axial growth. Whether there are reasons other than central corneal thinning and choroidal thickening needs further study.
MeSH term(s)	Axial Length, Eye ; Child ; Contact Lenses ; Corneal Topography ; Humans ; Orthokeratologic Procedures ; Refraction, Ocular ; Retrospective Studies
Language	English
Publishing date	2022-02-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2050436-6
ISSN	1471-2415 ; 1471-2415
ISSN (online)	1471-2415
ISSN	1471-2415
DOI	10.1186/s12886-022-02294-1
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Article ; Online: Engineered

Zeng, Yujing / Mu, Zheying / Nie, Beibei / Qu, Xinyu / Zhang, Yuanyuan / Li, Chao / Sun, Lizhou / Li, Genxi

Nano letters

2023 Volume 23, Issue 7, Page(s) 2854–2861

Abstract: Micro/nanocarriers hold great potential in bioanalysis for molecular recognition and signal amplification but are frequently hampered by harsh synthesis conditions and time-consuming labeling processes. Herein, we demonstrate ... ...

Abstract	Micro/nanocarriers hold great potential in bioanalysis for molecular recognition and signal amplification but are frequently hampered by harsh synthesis conditions and time-consuming labeling processes. Herein, we demonstrate that
MeSH term(s)	Humans ; Immunoassay ; Biosensing Techniques ; Limit of Detection ; Escherichia coli ; Delayed-Action Preparations
Chemical Substances	Delayed-Action Preparations
Language	English
Publishing date	2023-03-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1530-6992
ISSN (online)	1530-6992
DOI	10.1021/acs.nanolett.3c00184
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Article ; Online: Open-source lower controller for twelve degrees of freedom hydraulic quadruped robot with distributed control scheme

Lizhou Fang / Junhui Zhang / Huaizhi Zong / Ximeng Wang / Kun Zhang / Jun Shen / Zhenyu Lu

HardwareX, Vol 13, Iss , Pp e00393- (2023)

2023

Abstract: Nowadays, hydraulic quadruped robot shows high power density, good impact resistance and robustness in the research. The controller is the key to realize these features. This paper shows the design of an open-source single-leg controller for the ... ...

Abstract	Nowadays, hydraulic quadruped robot shows high power density, good impact resistance and robustness in the research. The controller is the key to realize these features. This paper shows the design of an open-source single-leg controller for the hydraulic quadruped robot Spurlos using a distributed control scheme. The single-leg system of the hydraulic quadruped robot Spurlos contains three angle encoders, three servo valves and six pressure sensors, which has the same components as most single-leg systems. Through the chips designed in the controller, the signal can be received from the encoders and the sensors, meanwhile the signal can be delivered to the servo valves. The software part of the controller adopts the MBD (Model-Based Design) method, which can greatly improve the development efficiency. According to the experiments, the controller design is reasonable, stable operation, and can satisfy the requirements of the hydraulic quadruped robot for leg motion control. The controller designed in this paper provides a solution to the problem that there is no ready-made control board for hydraulic quadruped robot which have three degrees of freedom for each leg. It enables the control researches for hydraulic quadruped robots to be more easily implemented.
Keywords	Hydraulic quadruped robot ; Digital signal processing ; Controller ; Automatic code generation ; Science (General) ; Q1-390
Subject code	629
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article ; Online: N6-methyladenosine (m6A) Modification in Preeclampsia.

Zhang, Tingting / Jiang, Ziyan / Yang, Nana / Ge, Zhiping / Zuo, Qing / Huang, Shiyun / Sun, Lizhou

Reproductive sciences (Thousand Oaks, Calif.)

2023 Volume 30, Issue 11, Page(s) 3144–3152

Abstract: Recently, epitranscriptional modification of N6-methyladenosine (m6A) has received growing attention in the research on the pathogenesis of preeclampsia. Advances in m6A sequencing have revealed the molecular mechanism and importance of m6A modification. ...

Abstract	Recently, epitranscriptional modification of N6-methyladenosine (m6A) has received growing attention in the research on the pathogenesis of preeclampsia. Advances in m6A sequencing have revealed the molecular mechanism and importance of m6A modification. In addition, epitranscriptional modification of m6A is closely related to the metabolic processes of placental tissues and cells in preeclampsia. This article reviews the composition, mode of action, and bioinformatics analysis of m6A modification-related proteins, and their biological function in the progression of preeclampsia. The relationship between m6A modification and preeclampsia risk factors, such as diabetes, cardiovascular disease, obesity, and psychological stress, is summarized to provide new ideas for studying PE-targeting molecules.
MeSH term(s)	Female ; Pregnancy ; Humans ; Placenta ; Pre-Eclampsia ; Adenosine ; Cardiovascular Diseases
Chemical Substances	Adenosine (K72T3FS567)
Language	English
Publishing date	2023-06-07
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2276411-2
ISSN	1933-7205 ; 1933-7191
ISSN (online)	1933-7205
ISSN	1933-7191
DOI	10.1007/s43032-023-01250-8
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