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  1. Article ; Online: Flow Virometry: A Fluorescence-Based Approach to Enumerate Bacteriophages in Liquid Samples.

    Dlusskaya, Elena A / Dey, Rafik

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2738, Page(s) 175–184

    Abstract: Correctly designed flow cytometry (virometry) assays allow accurate detection and enumeration of viruses in water. However, rigorous controls and calibrators are needed to obtain quality data. In the absence of proper controls, the use of fluorescent ... ...

    Abstract Correctly designed flow cytometry (virometry) assays allow accurate detection and enumeration of viruses in water. However, rigorous controls and calibrators are needed to obtain quality data. In the absence of proper controls, the use of fluorescent dyes for virus enumeration can produce false positive signals and lead to the wrong estimation of total virus counts by misreporting colloid particles as virions. Here we describe a protocol that addresses the problems that might potentially confound virometry data accuracy.
    MeSH term(s) Bacteriophages ; Biological Assay ; Data Accuracy ; Flow Cytometry ; Fluorescent Dyes
    Chemical Substances Fluorescent Dyes
    Language English
    Publishing date 2023-11-15
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3549-0_11
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Legionella

    Dey, Rafik / Ashbolt, Nicholas J

    ACS ES&T water

    2020  Volume 1, Issue 1, Page(s) 13–14

    Language English
    Publishing date 2020-09-23
    Publishing country United States
    Document type Journal Article
    ISSN 2690-0637
    ISSN (online) 2690-0637
    DOI 10.1021/acsestwater.0c00151
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  3. Article: First evidence of free-living Naegleria species in recreational lakes of Alberta, Canada.

    Dey, Rafik / Dlusskaya, Elena / Oloroso, Mariem / Ashbolt, Nicholas J

    Journal of water and health

    2023  Volume 21, Issue 3, Page(s) 439–442

    Abstract: Rising temperatures are increasing environmental habitats for thermotolerant pathogens, such as the so-called 'brain-eating amoeba', Naegleria fowleri. To the best of our knowledge, however, Naegleria species have not been reported in environmental water ...

    Abstract Rising temperatures are increasing environmental habitats for thermotolerant pathogens, such as the so-called 'brain-eating amoeba', Naegleria fowleri. To the best of our knowledge, however, Naegleria species have not been reported in environmental water sources in Canada. We surveyed popular recreational lakes in Alberta, Canada during the summer bathing period to determine the presence or absence of Naegleria species. While N. fowleri was not isolated in this study, we identified other thermotolerant species, including Naegleria pagei, Naegleria gruberi, Naegleria jejuensis and Naegleria fultoni using culture-based methods, hence indicating the potential conditions to support N. fowleri. Ongoing monitoring and examination of water for pathogenic amoebae is recommended in order to assist in the public health management of water sources.
    MeSH term(s) Naegleria ; Lakes ; Alberta ; Naegleria fowleri ; Water
    Chemical Substances Water (059QF0KO0R)
    Language English
    Publishing date 2023-06-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 2123845-5
    ISSN 1996-7829 ; 1477-8920
    ISSN (online) 1996-7829
    ISSN 1477-8920
    DOI 10.2166/wh.2023.325
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: First evidence of free-living Naegleria species in recreational lakes of Alberta, Canada

    Rafik Dey / Elena Dlusskaya / Mariem Oloroso / Nicholas J. Ashbolt

    Journal of Water and Health, Vol 21, Iss 3, Pp 439-

    2023  Volume 442

    Abstract: Rising temperatures are increasing environmental habitats for thermotolerant pathogens, such as the so-called ‘brain-eating amoeba’, Naegleria fowleri. To the best of our knowledge, however, Naegleria species have not been reported in environmental water ...

    Abstract Rising temperatures are increasing environmental habitats for thermotolerant pathogens, such as the so-called ‘brain-eating amoeba’, Naegleria fowleri. To the best of our knowledge, however, Naegleria species have not been reported in environmental water sources in Canada. We surveyed popular recreational lakes in Alberta, Canada during the summer bathing period to determine the presence or absence of Naegleria species. While N. fowleri was not isolated in this study, we identified other thermotolerant species, including Naegleria pagei, Naegleria gruberi, Naegleria jejuensis and Naegleria fultoni using culture-based methods, hence indicating the potential conditions to support N. fowleri. Ongoing monitoring and examination of water for pathogenic amoebae is recommended in order to assist in the public health management of water sources. HIGHLIGHTS Survey of Naegleria species in recreational lakes in Alberta, Canada.; First evidence of thermotolerant Naegleria species detection in Canada.; Climate change and northern expansion of Naegleria species.;
    Keywords amoebae ; canadian lakes ; climate change ; legionella ; naegleria spp ; waterborne pathogens ; Public aspects of medicine ; RA1-1270
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher IWA Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: SARS-CoV-2 surrogate (Phi6) environmental persistence within free-living amoebae.

    Dey, Rafik / Dlusskaya, Elena / Ashbolt, Nicholas J

    Journal of water and health

    2022  Volume 20, Issue 1, Page(s) 83–91

    Abstract: The reported persistence of SARS-CoV-2 virions in aquatic environments highlights the need to better understand potential mechanisms that may prolong its dissemination. We evaluated the possibility that amoebae might serve as transport hosts by studying ... ...

    Abstract The reported persistence of SARS-CoV-2 virions in aquatic environments highlights the need to better understand potential mechanisms that may prolong its dissemination. We evaluated the possibility that amoebae might serve as transport hosts by studying the interaction of the enveloped bacteriophage Phi6, as a potential surrogated along with one of the most common amoebae in engineered aquatic environments, Vermamoeba vermiformis. Using microscopy, imaging flow cytometry and bacteriophage cell culture, our results imply that the SARS-CoV-2 surrogate triggers amoebic mitochondria and induced apoptosis to promote viral persistence in trophozoites. Furthermore, virus-infected amoebae were still infectious after 2 months within FLA cysts. These results suggest that amoebae could contribute to the environmental persistence of SARS-CoV-2, including disinfection processes. In addition, amoebae could be a successful model system for understanding respiratory virus-eukaryotic biology at the cellular and molecular levels.
    MeSH term(s) Amoeba ; Bacteriophages ; COVID-19 ; Humans ; SARS-CoV-2 ; Viruses
    Language English
    Publishing date 2022-01-31
    Publishing country England
    Document type Journal Article
    ZDB-ID 2123845-5
    ISSN 1996-7829 ; 1477-8920
    ISSN (online) 1996-7829
    ISSN 1477-8920
    DOI 10.2166/wh.2021.167
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 7513: Show issues

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  6. Article ; Online: SARS-CoV-2 surrogate (Phi6) environmental persistence within free-living amoebae

    Rafik Dey / Elena Dlusskaya / Nicholas J. Ashbolt

    Journal of Water and Health, Vol 20, Iss 1, Pp 83-

    2022  Volume 91

    Abstract: The reported persistence of SARS-CoV-2 virions in aquatic environments highlights the need to better understand potential mechanisms that may prolong its dissemination. We evaluated the possibility that amoebae might serve as transport hosts by studying ... ...

    Abstract The reported persistence of SARS-CoV-2 virions in aquatic environments highlights the need to better understand potential mechanisms that may prolong its dissemination. We evaluated the possibility that amoebae might serve as transport hosts by studying the interaction of the enveloped bacteriophage Phi6, as a potential surrogated along with one of the most common amoebae in engineered aquatic environments, Vermamoeba vermiformis. Using microscopy, imaging flow cytometry and bacteriophage cell culture, our results imply that the SARS-CoV-2 surrogate triggers amoebic mitochondria and induced apoptosis to promote viral persistence in trophozoites. Furthermore, virus-infected amoebae were still infectious after 2 months within FLA cysts. These results suggest that amoebae could contribute to the environmental persistence of SARS-CoV-2, including disinfection processes. In addition, amoebae could be a successful model system for understanding respiratory virus-eukaryotic biology at the cellular and molecular levels. HIGHLIGHTS Amoebic mitochondria serve as a support organelle platform for the SARS-CoV-2 surrogate persistence.; Long-term stability of RNA enveloped viruses in amoeba-forming cysts.; Free-living amoebae as a potential vector and environmental reservoir for RNA enveloped viruses.;
    Keywords apoptosis ; bacteriophage phi6 ; environmental persistence ; free-living amoebae ; mitochondria ; sars-cov-2 ; transmission ; Public aspects of medicine ; RA1-1270
    Subject code 333
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher IWA Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Extracellular amoebal-vesicles: potential transmission vehicles for respiratory viruses.

    Dey, Rafik / Folkins, Melanie A / Ashbolt, Nicholas J

    NPJ biofilms and microbiomes

    2021  Volume 7, Issue 1, Page(s) 25

    Abstract: Human respiratory syncytial virus (RSV) is a major cause of acute respiratory tract infections in children and immunocompromised adults worldwide. Here we report that amoebae-release respirable-sized vesicles containing high concentrations of infectious ... ...

    Abstract Human respiratory syncytial virus (RSV) is a major cause of acute respiratory tract infections in children and immunocompromised adults worldwide. Here we report that amoebae-release respirable-sized vesicles containing high concentrations of infectious RSV that persisted for the duration of the experiment. Given the ubiquity of amoebae in moist environments, our results suggest that extracellular amoebal-vesicles could contribute to the environmental persistence of respiratory viruses, including potential resistance to disinfection processes and thereby offering novel pathways for viral dissemination and transmission.
    MeSH term(s) Adult ; Amoeba/growth & development ; Amoeba/virology ; Child ; Extracellular Vesicles/virology ; HeLa Cells ; Humans ; Immunocompromised Host ; Models, Biological ; Respiratory Syncytial Virus Infections/transmission ; Respiratory Syncytial Virus, Human/pathogenicity ; Virus Replication
    Language English
    Publishing date 2021-03-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2817021-0
    ISSN 2055-5008 ; 2055-5008
    ISSN (online) 2055-5008
    ISSN 2055-5008
    DOI 10.1038/s41522-021-00201-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Extracellular amoebal-vesicles

    Rafik Dey / Melanie A. Folkins / Nicholas J. Ashbolt

    npj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-

    potential transmission vehicles for respiratory viruses

    2021  Volume 5

    Abstract: Abstract Human respiratory syncytial virus (RSV) is a major cause of acute respiratory tract infections in children and immunocompromised adults worldwide. Here we report that amoebae-release respirable-sized vesicles containing high concentrations of ... ...

    Abstract Abstract Human respiratory syncytial virus (RSV) is a major cause of acute respiratory tract infections in children and immunocompromised adults worldwide. Here we report that amoebae-release respirable-sized vesicles containing high concentrations of infectious RSV that persisted for the duration of the experiment. Given the ubiquity of amoebae in moist environments, our results suggest that extracellular amoebal-vesicles could contribute to the environmental persistence of respiratory viruses, including potential resistance to disinfection processes and thereby offering novel pathways for viral dissemination and transmission.
    Keywords Microbial ecology ; QR100-130
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Legionella Infection during and after the COVID-19 Pandemic

    Dey, Rafik / Ashbolt, Nicholas J.

    ACS ES&T Water ; ISSN 2690-0637

    2020  

    Keywords covid19
    Language English
    Publisher American Chemical Society (ACS)
    Publishing country us
    Document type Article ; Online
    DOI 10.1021/acsestwater.0c00151
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Interactions between Human Reovirus and Free-Living Amoebae: Implications for Enteric Virus Disinfection and Aquatic Persistence.

    Folkins, Melanie A / Dey, Rafik / Ashbolt, Nicholas J

    Environmental science & technology

    2020  Volume 54, Issue 16, Page(s) 10201–10206

    Abstract: Free-living amoebae (FLA) are ubiquitous protozoa in aquatic/soil habitats and known to resist various disinfection methods commonly used in drinking and wastewater treatment plants. Reoviruses are emerging as useful infectious enteric virus indicators ... ...

    Abstract Free-living amoebae (FLA) are ubiquitous protozoa in aquatic/soil habitats and known to resist various disinfection methods commonly used in drinking and wastewater treatment plants. Reoviruses are emerging as useful infectious enteric virus indicators of wastewater treatment efficacy. The possible enhanced protection FLA may provide reoviruses, however, has not been previously described. Using an infectious clinical reovirus isolate in coculture with three FLA, namely,
    MeSH term(s) Acanthamoeba ; Amoeba ; Disinfection ; Enterovirus ; Humans ; Water Microbiology
    Language English
    Publishing date 2020-07-27
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1520-5851
    ISSN (online) 1520-5851
    DOI 10.1021/acs.est.0c02896
    Database MEDical Literature Analysis and Retrieval System OnLINE

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