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Article ; Online: Detection of Pneumocystis jirovecii from Clinical Specimens Utilizing a TaqMan-Based Real-Time PCR Assay on the Luminex ARIES.

Albulushi, Kyle / Jung-Hynes, Brittney / Chen, Derrick

Current protocols

2021 Volume 1, Issue 4, Page(s) e95

Abstract: Pneumocystis jirovecii can cause severe pneumonia in immunocompromised patients, which can be life threatening if left untreated. Despite the widespread use of polymerase chain reaction (PCR) within the clinical laboratory setting, FDA-approved PCR ... ...

Abstract	Pneumocystis jirovecii can cause severe pneumonia in immunocompromised patients, which can be life threatening if left untreated. Despite the widespread use of polymerase chain reaction (PCR) within the clinical laboratory setting, FDA-approved PCR assays are not readily available for the detection of Pneumocystis from respiratory samples. Using the Luminex ARIES system-an open-channel, automated, sample-to-answer PCR platform-the cell division cycle 2 (cdc-2) gene can be targeted for the detection of Pneumocystis. This novel TaqMan-based, real-time PCR assay offers improved sensitivity compared to staining or immunofluorescence while reducing turnaround time and eliminating the challenges surrounding microscopic identification. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Primer/probe master mix preparation Basic Protocol 2: Positive control (cdc-2) plasmid preparation Basic Protocol 3: Mucus digestion Basic Protocol 4: Cell lysis Basic Protocol 5: Carrier RNA/proteinase K preparation Basic Protocol 6: Cassette assembly Basic Protocol 7: Running the assay Basic Protocol 8: Interpreting results.
MeSH term(s)	Bronchoalveolar Lavage Fluid ; Humans ; Immunocompromised Host ; Pneumocystis carinii/genetics ; Pneumonia, Pneumocystis/diagnosis ; Real-Time Polymerase Chain Reaction
Language	English
Publishing date	2021-05-20
Document type	Journal Article
ISSN	2691-1299
ISSN (online)	2691-1299
DOI	10.1002/cpz1.95
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Comparative study of Revogene GBS LB assay and GeneXpert GBS LB assay for the detection of group B Streptococcus in prenatal screening samples.

Choera, Tsokyi / Jung-Hynes, Brittney / Chen, Derrick J

BMC infectious diseases

2020 Volume 20, Issue 1, Page(s) 38

Abstract: Background: Group B Streptococcal (GBS) infections in the United States are a leading cause of meningitis and sepsis in newborns. The CDC therefore recommends GBS screening for all pregnant women at 35-37 weeks of gestation and administration of ... ...

Abstract	Background: Group B Streptococcal (GBS) infections in the United States are a leading cause of meningitis and sepsis in newborns. The CDC therefore recommends GBS screening for all pregnant women at 35-37 weeks of gestation and administration of intrapartum prophylaxis (in those that tested positive) as an effective means of controlling disease transmission. Several FDA approved molecular diagnostic tests are available for rapid and accurate detection of GBS in antepartum women. Method: In this study, we report a clinical comparison of the Xpert GBS LB assay and a novel FDA-cleared test, Revogene GBS LB assay. A total of 250 vaginal-rectal swabs from women undergoing prenatal screening were submitted to the University of Wisconsin's clinical microbiology laboratory for GBS testing. Results: We found 96.8% of samples were concordant between the two tests, while 3.2% were discordant with a positive percent agreement of 98.0% and a negative percent agreement of 96.5% between the Revogene GBS LB assay and the GeneXpert GBS LB assay. Conclusion: Overall, we report that both assays perform well for the detection of GBS colonization in pregnant women.
MeSH term(s)	DNA, Viral/analysis ; Diagnostic Tests, Routine/methods ; Female ; Genetic Techniques ; Humans ; Infectious Disease Transmission, Vertical/prevention & control ; Mass Screening/economics ; Mass Screening/methods ; Molecular Diagnostic Techniques/economics ; Molecular Diagnostic Techniques/methods ; Pregnancy ; Pregnancy Complications, Infectious/diagnosis ; Pregnancy Complications, Infectious/virology ; Pregnant Women ; Prenatal Diagnosis/methods ; Streptococcal Infections/diagnosis ; Streptococcal Infections/virology ; Streptococcus agalactiae/genetics ; Time Factors ; Vagina/virology
Chemical Substances	DNA, Viral
Language	English
Publishing date	2020-01-14
Publishing country	England
Document type	Comparative Study ; Journal Article
ISSN	1471-2334
ISSN (online)	1471-2334
DOI	10.1186/s12879-019-4756-y
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Article ; Online: Case report: isolation of

Feichtinger, Stuart / Lazar, Angela A / Luebbe, Megan A / Accola, Molly A / Jung-Hynes, Brittney D / Anderson, Patti J / Koglin, Kelly M / Schliesman, Karen S / Ehlenbach, William / Smith, Jeannina / Chen, Derrick J / Rehrauer, William M / Bailey, Adam L

Access microbiology

2023 Volume 5, Issue 9

Abstract: A patient suffered a non-fatal wet drowning in a freshwater lake and developed bacteraemia several days later. Blood culture grew a Gram-negative rod that could not be identified by matrix-assisted laser desorption/ionization time-of-flight mass ... ...

Abstract	A patient suffered a non-fatal wet drowning in a freshwater lake and developed bacteraemia several days later. Blood culture grew a Gram-negative rod that could not be identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). 16S ribosomal RNA sequencing of the isolate identified the microbe as
Language	English
Publishing date	2023-09-19
Publishing country	England
Document type	Case Reports
ISSN	2516-8290
ISSN (online)	2516-8290
DOI	10.1099/acmi.0.000533.v4
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Article ; Online: SIRT1 controls circadian clock circuitry and promotes cell survival: a connection with age-related neoplasms.

Jung-Hynes, Brittney / Ahmad, Nihal

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

2009 Volume 23, Issue 9, Page(s) 2803–2809

Abstract: Aging is believed to be a primary risk factor for cancer. Interestingly, the sirtuin family of class III histone deacetylases (HDACs) has been implicated in the regulation of longevity and may be a lost link between aging and cancer. SIRT1, a ... ...

Abstract	Aging is believed to be a primary risk factor for cancer. Interestingly, the sirtuin family of class III histone deacetylases (HDACs) has been implicated in the regulation of longevity and may be a lost link between aging and cancer. SIRT1, a nicotinamide adenine dinucleotide (NAD(+))-dependent sirtuin, has been shown to promote cell survival by inhibiting apoptosis or cellular senescence in mammalian cells. Recent studies have provided a link between the cellular metabolic function of SIRT1 and the circadian rhythm (controlled by a clock machinery), which, if deregulated, may lead to an increased risk for some cancers. Interestingly, the loss of the pineal hormone melatonin, a known regulator of circadian rhythm, has been shown to cause deregulation in the circadian rhythm machinery and an increase in susceptibility to cancer. On the basis of scientific evidence, we propose a hypothesis that SIRT1 inhibition will impart an antiproliferative response in age-related cancers via resynchronization of deregulated core clock circuitry at the cellular level. If this hypothesis is found valid, it may ultimately lead to the development of novel approaches toward management of age-related malignancies and possibly other diseases.
MeSH term(s)	Aging ; Animals ; Cell Survival ; Circadian Rhythm/physiology ; Humans ; Neoplasms/etiology ; Sirtuin 1 ; Sirtuins/physiology
Chemical Substances	SIRT1 protein, human (EC 3.5.1.-) ; Sirtuin 1 (EC 3.5.1.-) ; Sirtuins (EC 3.5.1.-)
Language	English
Publishing date	2009-05-13
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	639186-2
ISSN	1530-6860 ; 0892-6638
ISSN (online)	1530-6860
ISSN	0892-6638
DOI	10.1096/fj.09-129148
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Zs.A 2266: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Role of p53 in the anti-proliferative effects of Sirt1 inhibition in prostate cancer cells.

Jung-Hynes, Brittney / Ahmad, Nihal

Cell cycle (Georgetown, Tex.)

2009 Volume 8, Issue 10, Page(s) 1478–1483

Abstract: Prostate cancer (PCa), next only to skin cancer, is the most commonly occurring malignancy in men in the US. Aging is recognized as a major risk factor for this neoplasm as a man's chance for developing this disease significantly increases with ... ...

Abstract	Prostate cancer (PCa), next only to skin cancer, is the most commonly occurring malignancy in men in the US. Aging is recognized as a major risk factor for this neoplasm as a man's chance for developing this disease significantly increases with increasing age. Because aging is inevitable, Americans are living longer, and the existing treatments have not been able to manage this neoplasm, novel mechanism-based approaches are needed. We have recently shown that Sirt1, a sirtuin class III histone deacetylases (HDACs) originally linked to aging and longevity in yeast, was overexpressed in human PCa cells and PCa tissues obtained from patients. We also found that chemical inhibition and/or genetic knockdown of Sirt1 caused a FoxO1-mediated inhibition in the growth and viability of human PCa cells. Since p53 is a target for deacetylation by Sirt1, we wanted to determine the involvement of p53 in Sirt1 inhibition mediated responses in PCa. To achieve our objective, we utilized a pair of isogenic PCa cell lines viz. PC3 and PC3-p53, which differ only in p53 status. Our data demonstrated that Sirt1 inhibition caused a decrease in cell growth, cell viability and the colony formation ability of both cell lines. Further, Sirt1 inhibition resulted in an increase in FoxO1 acetylation and subsequent transcriptional activation in both cell types regardless of p53 status. However, an interesting observation of our study was that Sirt1 inhibition resulted in an increase in senescence in PC3-p53 cells whereas it resulted in an increase in apoptosis in PC3 cells. The results of this study compliment our previous study and suggest that Sirt1 inhibition may have different downstream targets in cells with active p53 versus cells where p53 is inactive.
MeSH term(s)	Cell Proliferation ; Humans ; Male ; Prostatic Neoplasms/genetics ; Prostatic Neoplasms/metabolism ; Prostatic Neoplasms/pathology ; Sirtuin 1 ; Sirtuins/genetics ; Sirtuins/metabolism ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Protein p53/metabolism
Chemical Substances	Tumor Suppressor Protein p53 ; SIRT1 protein, human (EC 3.5.1.-) ; Sirtuin 1 (EC 3.5.1.-) ; Sirtuins (EC 3.5.1.-)
Language	English
Publishing date	2009-05-13
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2146183-1
ISSN	1551-4005 ; 1538-4101 ; 1554-8627
ISSN (online)	1551-4005
ISSN	1538-4101 ; 1554-8627
DOI	10.4161/cc.8.10.8408
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Article ; Online: Streptococcus agalactiae

Tickler, Isabella A / Tenover, Fred C / Dewell, Scott / Le, Victoria M / Blackman, Rachel N / Goering, Richard V / Rogers, Amy E / Piwonka, Heather / Jung-Hynes, Brittney D / Chen, Derrick J / Loeffelholz, Michael J / Gnanashanmugam, Devasena / Baron, Ellen Jo

Journal of clinical microbiology

2019 Volume 57, Issue 4

Abstract: Surveillance of circulating microbial populations is critical for monitoring the performance of a molecular diagnostic test. In this study, we characterized 31 isolates ... ...

Abstract	Surveillance of circulating microbial populations is critical for monitoring the performance of a molecular diagnostic test. In this study, we characterized 31 isolates of
MeSH term(s)	Bacterial Proteins/genetics ; Bacteriological Techniques ; Electrophoresis, Gel, Pulsed-Field ; Genome, Bacterial/genetics ; Hemolysin Proteins/genetics ; Humans ; Ireland/epidemiology ; Molecular Diagnostic Techniques/standards ; Multilocus Sequence Typing ; Phylogeny ; Sequence Deletion ; Streptococcal Infections/epidemiology ; Streptococcal Infections/microbiology ; Streptococcus agalactiae/classification ; Streptococcus agalactiae/genetics ; United States/epidemiology
Chemical Substances	Bacterial Proteins ; Hemolysin Proteins
Language	English
Publishing date	2019-03-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	390499-4
ISSN	1098-660X ; 0095-1137
ISSN (online)	1098-660X
ISSN	0095-1137
DOI	10.1128/JCM.02040-18
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Article ; Online: Sirtuins, melatonin and circadian rhythms: building a bridge between aging and cancer.

Jung-Hynes, Brittney / Reiter, Russel J / Ahmad, Nihal

Journal of pineal research

2009 Volume 48, Issue 1, Page(s) 9–19

Abstract: Histone deacetylases (HDAC) have been under intense scientific investigation for a number of years. However, only recently the unique class III HDAC, sirtuins, have gained increasing investigational momentum. Originally linked to longevity in yeast, ... ...

Abstract	Histone deacetylases (HDAC) have been under intense scientific investigation for a number of years. However, only recently the unique class III HDAC, sirtuins, have gained increasing investigational momentum. Originally linked to longevity in yeast, sirtuins and more specifically, SIRT1 have been implicated in numerous biological processes having both protective and/or detrimental effects. SIRT1 appears to play a critical role in the process of carcinogenesis, especially in age-related neoplasms. Similarly, alterations in circadian rhythms as well as production of the pineal hormone melatonin have been linked to aging and cancer risk. Melatonin has been found act as a differentiating agent in some cancer cells and to lower their invasive and metastatic status. In addition, melatonin synthesis and release occurs in a circadian rhythm fashion and it has been linked to the core circadian machinery genes (Clock, Bmal1, Periods, and Cryptochromes). Melatonin has also been associated with chronotherapy, the timely administration of chemotherapy agents to optimize trends in biological cycles. Interestingly, a recent set of studies have linked SIRT1 to the circadian rhythm machinery through direct deacetylation activity as well as through the nicotinamide adenine dinucleotide (NAD(+)) salvage pathway. In this review, we provide evidence for a possible connection between sirtuins, melatonin, and the circadian rhythm circuitry and their implications in aging, chronomodulation, and cancer.
MeSH term(s)	Aging/metabolism ; Aging/physiology ; Animals ; Circadian Rhythm/physiology ; Humans ; Melatonin/metabolism ; Melatonin/physiology ; Models, Biological ; Neoplasms/metabolism ; Neoplasms/physiopathology ; Sirtuins/metabolism ; Sirtuins/physiology
Chemical Substances	Sirtuins (EC 3.5.1.-) ; Melatonin (JL5DK93RCL)
Language	English
Publishing date	2009-12-15
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Review
ZDB-ID	632697-3
ISSN	1600-079X ; 0742-3098
ISSN (online)	1600-079X
ISSN	0742-3098
DOI	10.1111/j.1600-079X.2009.00729.x
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Article: SIRT1 controls circadian clock circuitry and promotes cell survival: a connection with age-related neoplasms

Jung-Hynes, Brittney / Ahmad, Nihal

FASEB journal. 2009 Sept., v. 23, no. 9

2009

Abstract: ... Jung-Hynes, B., Ahmad, N. SIRT1 controls circadian clock circuitry and promotes cell survival ...

Abstract	Aging is believed to be a primary risk factor for cancer. Interestingly, the sirtuin family of class III histone deacetylases (HDACs) has been implicated in the regulation of longevity and may be a lost link between aging and cancer. SIRT1, a nicotinamide adenine dinucleotide (NAD⁺)-dependent sirtuin, has been shown to promote cell survival by inhibiting apoptosis or cellular senescence in mammalian cells. Recent studies have provided a link between the cellular metabolic function of SIRT1 and the circadian rhythm (controlled by a clock machinery), which, if deregulated, may lead to an increased risk for some cancers. Interestingly, the loss of the pineal hormone melatonin, a known regulator of circadian rhythm, has been shown to cause deregulation in the circadian rhythm machinery and an increase in susceptibility to cancer. On the basis of scientific evidence, we propose a hypothesis that SIRT1 inhibition will impart an antiproliferative response in age-related cancers via resynchronization of deregulated core clock circuitry at the cellular level. If this hypothesis is found valid, it may ultimately lead to the development of novel approaches toward management of age-related malignancies and possibly other diseases.--Jung-Hynes, B., Ahmad, N. SIRT1 controls circadian clock circuitry and promotes cell survival: a connection with age-related neoplasms.
Language	English
Dates of publication	2009-09
Size	p. 2803-2809.
Publishing place	The Federation of American Societies for Experimental Biology
Document type	Article
ZDB-ID	639186-2
ISSN	1530-6860 ; 0892-6638
ISSN (online)	1530-6860
ISSN	0892-6638
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Article ; Online: Melatonin resynchronizes dysregulated circadian rhythm circuitry in human prostate cancer cells.

Jung-Hynes, Brittney / Huang, Wei / Reiter, Russel J / Ahmad, Nihal

Journal of pineal research

2010 Volume 49, Issue 1, Page(s) 60–68

Abstract: Prostate cancer (PCa) is a major age-related malignancy as increasing age correlates with increased risk for developing this neoplasm. Similarly, alterations in circadian rhythms have also been associated with the aging population and cancer risk. The ... ...

Abstract	Prostate cancer (PCa) is a major age-related malignancy as increasing age correlates with increased risk for developing this neoplasm. Similarly, alterations in circadian rhythms have also been associated with the aging population and cancer risk. The pineal hormone melatonin is known to regulate circadian rhythms, which is under the control of a core set of genes: Period 1, 2, 3 (Per 1-3); Cryptochrome 1, 2 (Cry 1, 2); Clock, and Bmal 1, 2. Melatonin levels have been shown to decrease in patients with cancer and exogenous melatonin exhibits antiproliferative effects against certain cancers. In this study, we challenged the hypothesis that melatonin imparts antiproliferative effects in prostate cancer via resynchronization of deregulated core clock circuitry. We found that Clock and Per2 protein levels were downregulated whereas Bmal1 protein levels were upregulated in PCa cells, compared to normal prostate cells. Additionally, employing automated quantitative analysis of a microarray containing human tissues, we found that compared to benign tissues, Clock and Per2 levels were downregulated, whereas Bmal1 levels were upregulated in PCa and other proliferative prostatic conditions. Overexpression of Per2 was found to result in a significant loss of PCa cell growth and viability. Interestingly, melatonin treatment resulted in an increase in Per2 and Clock and a reduction in Bmal1 in PCa cells. Further, melatonin treatment resulted in a resynchronization of oscillatory circadian rhythm genes (Dbp and Per2). Our data support our hypothesis and suggest that melatonin should be thoroughly investigated as an agent for the management of PCa and other age-related malignancies.
MeSH term(s)	ARNTL Transcription Factors/genetics ; ARNTL Transcription Factors/metabolism ; Apoptosis/drug effects ; Blotting, Western ; CLOCK Proteins/genetics ; CLOCK Proteins/metabolism ; Cell Line, Tumor ; Circadian Rhythm/drug effects ; DNA-Binding Proteins/metabolism ; Gene Expression/drug effects ; Gene Expression Profiling ; Humans ; Male ; Melatonin/pharmacology ; Period Circadian Proteins/genetics ; Period Circadian Proteins/metabolism ; Poly(ADP-ribose) Polymerases/metabolism ; Prostatic Neoplasms/genetics ; Prostatic Neoplasms/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Tissue Array Analysis ; Transcription Factors/metabolism
Chemical Substances	ARNTL Transcription Factors ; BMAL1 protein, human ; DBP protein, human ; DNA-Binding Proteins ; PER2 protein, human ; Period Circadian Proteins ; Transcription Factors ; CLOCK Proteins (EC 2.3.1.48) ; CLOCK protein, human (EC 2.3.1.48) ; Poly(ADP-ribose) Polymerases (EC 2.4.2.30) ; Melatonin (JL5DK93RCL)
Language	English
Publishing date	2010-05-27
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	632697-3
ISSN	1600-079X ; 0742-3098
ISSN (online)	1600-079X
ISSN	0742-3098
DOI	10.1111/j.1600-079X.2010.00767.x
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Article: Role of sirtuin histone deacetylase SIRT1 in prostate cancer. A target for prostate cancer management via its inhibition?

Jung-Hynes, Brittney / Nihal, Minakshi / Zhong, Weixiong / Ahmad, Nihal

The Journal of biological chemistry

2008 Volume 284, Issue 6, Page(s) 3823–3832

Abstract: Prostate cancer (PCa) is a major age-related malignancy, and according to estimates from the American Cancer Society, a man's chance of developing this cancer significantly increases with increasing age, from 1 in 10,149 by age 39 to 1 in 38 by age 59 to ...

Abstract	Prostate cancer (PCa) is a major age-related malignancy, and according to estimates from the American Cancer Society, a man's chance of developing this cancer significantly increases with increasing age, from 1 in 10,149 by age 39 to 1 in 38 by age 59 to 1 in 7 by age 70. Therefore, it is important to identify the causal connection between mechanisms of aging and PCa. Employing in vitro and in vivo approaches, in this study, we tested the hypothesis that SIRT1, which belongs to the Sir2 (silent information regulator 2) family of sirtuin class III histone deacetylases, is overexpressed in PCa, and its inhibition will have antiproliferative effects in human PCa cells. Our data demonstrated that SIRT1 was significantly overexpressed in human PCa cells (DU145, LNCaP, 22Rnu1, and PC3) compared with normal prostate epithelial cells (PrEC) at protein, mRNA, and enzymatic activity levels. SIRT1 was also found to be overexpressed in human PCa tissues compared with adjacent normal prostate tissue. Interestingly, our data demonstrated that SIRT1 inhibition via nicotinamide and sirtinol (at the activity level) as well as via short hairpin RNA-mediated RNA interference (at the genetic level) resulted in a significant inhibition in the growth and viability of human PCa cells while having no effect on normal prostate epithelial cells. Further, we found that inhibition of SIRT1 caused an increase in FOXO1 acetylation and transcriptional activation in PCa cells. Our data suggested that SIRT1, via inhibiting FOXO1 activation, could contribute to the development of PCa. We suggest that SIRT1 could serve as a target toward developing novel strategies for PCa management.
MeSH term(s)	Adult ; Aged ; Benzamides/pharmacology ; Cell Line, Tumor ; Forkhead Box Protein O1 ; Forkhead Transcription Factors/metabolism ; Gene Expression Regulation, Developmental/drug effects ; Gene Expression Regulation, Neoplastic/drug effects ; Histone Deacetylase Inhibitors ; Histone Deacetylases/biosynthesis ; Humans ; Male ; Middle Aged ; Naphthols/pharmacology ; Neoplasm Proteins/biosynthesis ; Niacinamide/pharmacology ; Prostatic Neoplasms/enzymology ; Prostatic Neoplasms/pathology ; Prostatic Neoplasms/therapy ; Sirtuin 1 ; Sirtuins/antagonists & inhibitors ; Sirtuins/biosynthesis ; Vitamin B Complex/pharmacology
Chemical Substances	Benzamides ; FOXO1 protein, human ; Forkhead Box Protein O1 ; Forkhead Transcription Factors ; Histone Deacetylase Inhibitors ; Naphthols ; Neoplasm Proteins ; sirtinol ; Vitamin B Complex (12001-76-2) ; Niacinamide (25X51I8RD4) ; SIRT1 protein, human (EC 3.5.1.-) ; Sirtuin 1 (EC 3.5.1.-) ; Sirtuins (EC 3.5.1.-) ; Histone Deacetylases (EC 3.5.1.98)
Language	English
Publishing date	2008-12-15
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
DOI	10.1074/jbc.M807869200
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