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  1. Article ; Online: Will the latest wave of the COVID-19 pandemic be an ecological disaster? There is an urgent need to replace plastic by ecologically virtuous materials.

    Mansuy, Jean-Michel / Migueres, Marion / Trémeaux, Pauline / Izopet, Jacques

    Health science reports

    2022  Volume 5, Issue 5, Page(s) e703

    Abstract: Background and aims: Direct virological diagnosis of Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2) infectionis based on either viral antigen or viral genome detection. These methods, in addition to the dedicated reagents and transport ... ...

    Abstract Background and aims: Direct virological diagnosis of Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2) infectionis based on either viral antigen or viral genome detection. These methods, in addition to the dedicated reagents and transport packaging, require the use of quantities of plastic that may individually appear negligible but which, in the context of a pandemic, are very high. The aim was to estimate the amount of plastic involved in a diagnostic assay whether molecular or antigenic.
    Methods: We weighed the plastics used to obtain a diagnostic assay result for SARS-CoV-2 infection in our hospital.
    Results: Each ready-to-use antigen assay requires about 20 g of plastic whereas the PCR assay implies the use of 30 g. This unit mass, when compared to our laboratory's SARS-CoV-2 genomic screening activity,represents more than 10 tons of plastic for 2021. At our region level (#6.10 inhabitants), more than 350 tons of plastic were used to carry out more than 7 million declared PCR assays and as many antigenic assays.
    Conclusions: The virologic diagnostic activityl inked to the SARS-CoV-2 pandemic has highlighted once more our dependance for plastic use. We must already think about a more environmentally virtuous diagnostic activity by integrating a reasonned use of diagnostic tools and a higher use of ecological friendly material. Parallel the notion of waste management must also be addressed in order to limit their environmental impact.
    Language English
    Publishing date 2022-08-07
    Publishing country United States
    Document type Journal Article
    ISSN 2398-8835
    ISSN (online) 2398-8835
    DOI 10.1002/hsr2.703
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Hepatitis A: Epidemiology, High-Risk Groups, Prevention and Research on Antiviral Treatment.

    Migueres, Marion / Lhomme, Sébastien / Izopet, Jacques

    Viruses

    2021  Volume 13, Issue 10

    Abstract: The hepatitis A virus (HAV) is a leading cause of acute viral hepatitis worldwide. It is transmitted mainly by direct contact with patients who have been infected or by ingesting contaminated water or food. The virus is endemic in low-income countries ... ...

    Abstract The hepatitis A virus (HAV) is a leading cause of acute viral hepatitis worldwide. It is transmitted mainly by direct contact with patients who have been infected or by ingesting contaminated water or food. The virus is endemic in low-income countries where sanitary and sociodemographic conditions are poor. Paradoxically, improving sanitary conditions in these countries, which reduces the incidence of HAV infections, can lead to more severe disease in susceptible adults. The populations of developed countries are highly susceptible to HAV, and large outbreaks can occur when the virus is spread by globalization and by increased travel and movement of foodstuffs. Most of these outbreaks occur among high-risk groups: travellers, men who have sex with men, people who use substances, and people facing homelessness. Hepatitis A infections can be prevented by vaccination; safe and effective vaccines have been available for decades. Several countries have successfully introduced universal mass vaccination for children, but high-risk groups in high-income countries remain insufficiently protected. The development of HAV antivirals may be important to control HAV outbreaks in developed countries where a universal vaccination programme is not recommended.
    MeSH term(s) Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Disease Outbreaks/prevention & control ; Hepatitis A/drug therapy ; Hepatitis A/epidemiology ; Hepatitis A/prevention & control ; Hepatitis A virus/drug effects ; Homosexuality, Male ; Humans ; Incidence ; Male ; Risk Factors ; Sexual and Gender Minorities ; Travel
    Chemical Substances Antiviral Agents
    Language English
    Publishing date 2021-09-22
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13101900
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Hepatitis A: Epidemiology, High-Risk Groups, Prevention and Research on Antiviral Treatment

    Migueres, Marion / Lhomme, Sébastien / Izopet, Jacques

    Viruses. 2021 Sept. 22, v. 13, no. 10

    2021  

    Abstract: The hepatitis A virus (HAV) is a leading cause of acute viral hepatitis worldwide. It is transmitted mainly by direct contact with patients who have been infected or by ingesting contaminated water or food. The virus is endemic in low-income countries ... ...

    Abstract The hepatitis A virus (HAV) is a leading cause of acute viral hepatitis worldwide. It is transmitted mainly by direct contact with patients who have been infected or by ingesting contaminated water or food. The virus is endemic in low-income countries where sanitary and sociodemographic conditions are poor. Paradoxically, improving sanitary conditions in these countries, which reduces the incidence of HAV infections, can lead to more severe disease in susceptible adults. The populations of developed countries are highly susceptible to HAV, and large outbreaks can occur when the virus is spread by globalization and by increased travel and movement of foodstuffs. Most of these outbreaks occur among high-risk groups: travellers, men who have sex with men, people who use substances, and people facing homelessness. Hepatitis A infections can be prevented by vaccination; safe and effective vaccines have been available for decades. Several countries have successfully introduced universal mass vaccination for children, but high-risk groups in high-income countries remain insufficiently protected. The development of HAV antivirals may be important to control HAV outbreaks in developed countries where a universal vaccination programme is not recommended.
    Keywords Hepatovirus A ; antiviral agents ; direct contact ; disease severity ; globalization ; hepatitis A ; travel ; vaccination ; viruses ; water pollution
    Language English
    Dates of publication 2021-0922
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13101900
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Vectorial Release of Human RNA Viruses from Epithelial Cells.

    Chapuy-Regaud, Sabine / Allioux, Claire / Capelli, Nicolas / Migueres, Marion / Lhomme, Sébastien / Izopet, Jacques

    Viruses

    2022  Volume 14, Issue 2

    Abstract: Epithelial cells are apico-basolateral polarized cells that line all tubular organs and are often targets for infectious agents. This review focuses on the release of human RNA virus particles from both sides of polarized human cells grown on transwells. ...

    Abstract Epithelial cells are apico-basolateral polarized cells that line all tubular organs and are often targets for infectious agents. This review focuses on the release of human RNA virus particles from both sides of polarized human cells grown on transwells. Most viruses that infect the mucosa leave their host cells mainly via the apical side while basolateral release is linked to virus propagation within the host. Viruses do this by hijacking the cellular factors involved in polarization and trafficking. Thus, understanding epithelial polarization is essential for a clear understanding of virus pathophysiology.
    MeSH term(s) Cell Polarity ; Epithelial Cells/virology ; Humans ; RNA Viruses/physiology ; Virion/physiology ; Virus Assembly ; Virus Release ; Virus Replication
    Language English
    Publishing date 2022-01-25
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14020231
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Will the latest wave of the COVID‐19 pandemic be an ecological disaster? There is an urgent need to replace plastic by ecologically virtuous materials

    Jean‐Michel Mansuy / Marion Migueres / Pauline Trémeaux / Jacques Izopet

    Health Science Reports, Vol 5, Iss 5, Pp n/a-n/a (2022)

    2022  

    Abstract: Abstract Background and Aims Direct virological diagnosis of Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS‐CoV‐2) infectionis based on either viral antigen or viral genome detection. These methods, in addition to the dedicated reagents and ... ...

    Abstract Abstract Background and Aims Direct virological diagnosis of Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS‐CoV‐2) infectionis based on either viral antigen or viral genome detection. These methods, in addition to the dedicated reagents and transport packaging, require the use of quantities of plastic that may individually appear negligible but which, in the context of a pandemic, are very high. The aim was to estimate the amount of plastic involved in a diagnostic assay whether molecular or antigenic. Methods We weighed the plastics used to obtain a diagnostic assay result for SARS‐CoV‐2 infection in our hospital. Results Each ready‐to‐use antigen assay requires about 20 g of plastic whereas the PCR assay implies the use of 30 g. This unit mass, when compared to our laboratory's SARS‐CoV‐2 genomic screening activity,represents more than 10 tons of plastic for 2021. At our region level (#6.10 inhabitants), more than 350 tons of plastic were used to carry out more than 7 million declared PCR assays and as many antigenic assays. Conclusions The virologic diagnostic activityl inked to the SARS‐CoV‐2 pandemic has highlighted once more our dependance for plastic use. We must already think about a more environmentally virtuous diagnostic activity by integrating a reasonned use of diagnostic tools and a higher use of ecological friendly material. Parallel the notion of waste management must also be addressed in order to limit their environmental impact.
    Keywords antigen ; COVID‐19 ; diagnosis ; ecology ; PCR ; plastic ; Medicine ; R
    Subject code 621
    Language English
    Publishing date 2022-09-01T00:00:00Z
    Publisher Wiley
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article: Hepatitis E Virus: How It Escapes Host Innate Immunity.

    Lhomme, Sébastien / Migueres, Marion / Abravanel, Florence / Marion, Olivier / Kamar, Nassim / Izopet, Jacques

    Vaccines

    2020  Volume 8, Issue 3

    Abstract: Hepatitis E virus (HEV) is a leading cause of viral hepatitis in the world. It is usually responsible for acute hepatitis, but can lead to a chronic infection in immunocompromised patients. The host's innate immune response is the first line of defense ... ...

    Abstract Hepatitis E virus (HEV) is a leading cause of viral hepatitis in the world. It is usually responsible for acute hepatitis, but can lead to a chronic infection in immunocompromised patients. The host's innate immune response is the first line of defense against a virus infection; there is growing evidence that HEV RNA is recognized by toll-like receptors (TLRs) and retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs), leading to interferon (IFN) production. The IFNs activate interferon-stimulated genes (ISGs) to limit HEV replication and spread. HEV has developed strategies to counteract this antiviral response, by limiting IFN induction and signaling. This review summarizes the advances in our knowledge of intracellular pathogen recognition, interferon and inflammatory response, and the role of virus protein in immune evasion.
    Language English
    Publishing date 2020-07-28
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines8030422
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Evaluation of two anti-SARS-CoV-2 antibody immunoassays for monitoring patients on pre-exposure prophylaxis.

    Vellas, Camille / Dimeglio, Chloé / Joncour, Emma / Staes, Laetitia / Jamme, Thibaut / Miedougé, Marcel / Da-Silva, Isabelle / Porcheron, Marion / Migueres, Marion / Kamar, Nassim / Izopet, Jacques

    Diagnostic microbiology and infectious disease

    2023  Volume 107, Issue 4, Page(s) 116071

    Abstract: Pre-exposure prophylaxis (PrEP) is crucial to prevent severe COVID-19 in immunocompromised patients. A reliable method is needed to quantify anti-SARS-CoV-2 antibody levels for personalized monitoring during PrEP. We measured the binding antibody ... ...

    Abstract Pre-exposure prophylaxis (PrEP) is crucial to prevent severe COVID-19 in immunocompromised patients. A reliable method is needed to quantify anti-SARS-CoV-2 antibody levels for personalized monitoring during PrEP. We measured the binding antibody concentrations of 63 immunocompromised patients receiving 300mg or 600mg tixagevimab/cilgavimab on PrEP day and twice during the following 3 months. All blood samples were tested using the Abbott anti-SARS-CoV-2 IgG II Quant assay, the Roche Elecsys anti-SARS-CoV-2 S assay, and live virus-based neutralization assays. The results of the two immunoassays were correlated on day 0, 1 month, and 3 months post-PrEP. Passing-Bablok regression demonstrated higher anti-S concentration values measured with the Roche immunoassay compared to those measured with the Abbott immunoassay. Antibody concentrations were higher after 600 mg tixagevimab/cilgavimab prophylaxis than after 300 mg. The neutralizing antibody titers obtained using the omicron BA.5 and BA.2.75 strains were low. Both automated immunoassays are suitable for monitoring immunocompromised patients on PrEP.
    MeSH term(s) Humans ; Pre-Exposure Prophylaxis ; COVID-19/diagnosis ; COVID-19/prevention & control ; Antibodies, Viral ; Immunoassay ; Biological Assay
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2023-08-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604920-5
    ISSN 1879-0070 ; 0732-8893
    ISSN (online) 1879-0070
    ISSN 0732-8893
    DOI 10.1016/j.diagmicrobio.2023.116071
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Influence of immune escape and nasopharyngeal virus load on the spread of SARS-CoV-2 Omicron variant.

    Migueres, Marion / Dimeglio, Chloé / Trémeaux, Pauline / Abravanel, Florence / Raymond, Stéphanie / Lhomme, Sébastien / Mansuy, Jean-Michel / Izopet, Jacques

    The Journal of infection

    2022  Volume 84, Issue 4, Page(s) e7–e9

    MeSH term(s) COVID-19 ; Humans ; SARS-CoV-2 ; Serologic Tests ; Spike Glycoprotein, Coronavirus
    Chemical Substances Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2022-02-07
    Publishing country England
    Document type Letter
    ZDB-ID 424417-5
    ISSN 1532-2742 ; 0163-4453
    ISSN (online) 1532-2742
    ISSN 0163-4453
    DOI 10.1016/j.jinf.2022.01.036
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Antibody titers and breakthrough infections with Omicron SARS-CoV-2.

    Dimeglio, Chloé / Migueres, Marion / Mansuy, Jean-Michel / Saivin, Sylvie / Miedougé, Marcel / Chapuy-Regaud, Sabine / Izopet, Jacques

    The Journal of infection

    2022  Volume 84, Issue 4, Page(s) e13–e15

    MeSH term(s) Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 ; Humans ; Immunologic Tests ; SARS-CoV-2
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral
    Language English
    Publishing date 2022-02-03
    Publishing country England
    Document type Letter
    ZDB-ID 424417-5
    ISSN 1532-2742 ; 0163-4453
    ISSN (online) 1532-2742
    ISSN 0163-4453
    DOI 10.1016/j.jinf.2022.01.044
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Comparative effects of mRNA vaccine booster and natural Omicron infection on the neutralizing antibody response.

    Dimeglio, Chloé / Migueres, Marion / Chapuy-Regaud, Sabine / Da-Silva, Isabelle / Jougla, Isabelle / Pradere, Claire / Porcheron, Marion / Martin-Blondel, Guillaume / Lougarre, Catherine / Herin, Fabrice / Izopet, Jacques

    The Journal of infection

    2022  Volume 85, Issue 1, Page(s) e4–e6

    MeSH term(s) Antibodies, Neutralizing ; Antibodies, Viral ; Antibody Formation ; Humans ; Vaccines, Synthetic ; mRNA Vaccines
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral ; Vaccines, Synthetic ; mRNA Vaccines
    Language English
    Publishing date 2022-05-07
    Publishing country England
    Document type Letter
    ZDB-ID 424417-5
    ISSN 1532-2742 ; 0163-4453
    ISSN (online) 1532-2742
    ISSN 0163-4453
    DOI 10.1016/j.jinf.2022.05.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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