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Article: Sex, aging and immunity in multiple sclerosis and experimental autoimmune encephalomyelitis: An intriguing interaction.

Boziki, Marina / Theotokis, Paschalis / Kesidou, Evangelia / Karafoulidou, Eleni / Konstantinou, Chrystalla / Michailidou, Iliana / Bahar, Yasemin / Altintas, Ayse / Grigoriadis, Nikolaos

Frontiers in neurology

2023 Volume 13, Page(s) 1104552

Abstract: Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) with a profound neurodegenerative component early in the disease pathogenesis. Age is a factor with a well-described effect on the primary disease phenotype, ... ...

Abstract	Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) with a profound neurodegenerative component early in the disease pathogenesis. Age is a factor with a well-described effect on the primary disease phenotype, namely, the relapsing-remitting vs. the primary progressive disease. Moreover, aging is a prominent factor contributing to the transition from relapsing-remitting MS (RRMS) to secondary progressive disease. However, sex also seems to, at least in part, dictate disease phenotype and evolution, as evidenced in humans and in animal models of the disease. Sex-specific gene expression profiles have recently elucidated an association with differential immunological signatures in the context of experimental disease. This review aims to summarize current knowledge stemming from experimental autoimmune encephalomyelitis (EAE) models regarding the effects of sex, either independently or as a factor combined with aging, on disease phenotype, with relevance to the immune system and the CNS.
Language	English
Publishing date	2023-01-09
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2564214-5
ISSN	1664-2295
ISSN	1664-2295
DOI	10.3389/fneur.2022.1104552
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Article ; Online: Salivary diagnostics of the novel coronavirus SARS-CoV-2 (COVID-19).

Michailidou, Evangelia / Poulopoulos, Athanasios / Tzimagiorgis, Georgios

Oral diseases

2020 Volume 28 Suppl 1, Page(s) 867–877

Abstract: Introduction: Laboratory testing for the SARS-CoV-2 virus and the consequent respiratory coronavirus disease 2019 (COVID-19) is categorized into methods that detect the viral presence and methods that detect antibodies produced in the host as a response ...

Abstract	Introduction: Laboratory testing for the SARS-CoV-2 virus and the consequent respiratory coronavirus disease 2019 (COVID-19) is categorized into methods that detect the viral presence and methods that detect antibodies produced in the host as a response to infection. Methods that detect viral presence into the host excretions measure current infection by SARS-CoV-2, whereas the detection of human antibodies exploited against SARS-CoV-2 evaluates the past exposure to the virus. Objective: This review provides a comprehensive overview for the use of saliva as a specimen for the detection of SARS-CoV-2, the methods for the salivary diagnostics utilized till very recently, and the arisen considerations for the diagnosis of COVID-19 disease. Conclusion: The major advantage of using saliva as a specimen for the detection of SARS-CoV-2 is that saliva collection is a non-invasive method which produces no discomfort to the patient and permits the patients to utilize home self-sampling techniques in order to protect health providers from the exposure to the pathogen. There is an urgent need to increase the active research for the detection of SARS-CoV-2 in the saliva because the non-invasive salivary diagnostics may provide a reliable and cost-effective method suitable for the fast and early detection of COVID-19 infection.
MeSH term(s)	COVID-19/diagnosis ; Humans ; SARS-CoV-2 ; Saliva
Keywords	covid19
Language	English
Publishing date	2020-12-04
Publishing country	Denmark
Document type	Journal Article ; Review
ZDB-ID	1290529-x
ISSN	1601-0825 ; 1354-523X
ISSN (online)	1601-0825
ISSN	1354-523X
DOI	10.1111/odi.13729
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Article ; Online: Effectiveness of COVID-19 vaccination against school absenteeism in children and adolescents hospitalized with COVID-19.

Maltezou, Helena C / Kontogianni, Stavroula / Michailidou, Elisavet / Vergadi, Eleni / Giannouchos, Theodoros V / Steletou, Evangelia / Sipsas, Nikolaos V / Galanakis, Emmanouil / Syrogiannopoulos, George A / Roilides, Emmanuel

Vaccine

2024 Volume 42, Issue 12, Page(s) 2941–2944

Abstract: Background: COVID-19 vaccination has been recommended for children to protect them and to enable in-person educational and social activities.: Methods: We estimated COVID-19 vaccination effectiveness (VE) against school absenteeism in children 5-17 ... ...

Abstract	Background: COVID-19 vaccination has been recommended for children to protect them and to enable in-person educational and social activities. Methods: We estimated COVID-19 vaccination effectiveness (VE) against school absenteeism in children 5-17 years old hospitalized from September 1, 2021 through May 31, 2023. Full vaccination was defined as two vaccine doses. Results: We studied 231 children admitted to hospital with COVID-19, including 206 (89.2 %) unvaccinated/partially vaccinated and 25 (10.8 %) fully vaccinated. Unvaccinated/partially vaccinated children were absent from school for longer periods compared to fully vaccinated children (median absence: 14 versus 10 days; p-value = 0.05). Multivariable regression showed that full COVID-19 vaccination was associated with fewer days of absence compared to no/partial vaccination on average (adjusted relative risk: 0.77; 95 % CI: 0.61 to 0.98). COVID-19 VE was 50.7 % (95 % CI: -11.3 % to 78.2 %) for school absenteeism above the median duration of absenteeism. Conclusions: Full COVID-19 vaccination conferred protection against school absenteeism in hospitalized school-aged children with COVID-19.
MeSH term(s)	Child ; Humans ; Adolescent ; Child, Preschool ; Influenza Vaccines ; COVID-19 Vaccines ; Influenza, Human/prevention & control ; Absenteeism ; COVID-19/prevention & control ; Vaccination
Chemical Substances	Influenza Vaccines ; COVID-19 Vaccines
Language	English
Publishing date	2024-03-30
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2024.03.075
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Article: The Time Trajectory of Choroid Plexus Enlargement in Multiple Sclerosis.

Andravizou, Athina / Stavropoulou De Lorenzo, Sotiria / Kesidou, Evangelia / Michailidou, Iliana / Parissis, Dimitrios / Boziki, Marina-Kleopatra / Stamati, Polyxeni / Bakirtzis, Christos / Grigoriadis, Nikolaos

Healthcare (Basel, Switzerland)

2024 Volume 12, Issue 7

Abstract: Choroid plexus (CP) can be seen as a watchtower of the central nervous system (CNS) that actively regulates CNS homeostasis. A growing body of literature suggests that CP alterations are involved in the pathogenesis of multiple sclerosis (MS) but the ... ...

Abstract	Choroid plexus (CP) can be seen as a watchtower of the central nervous system (CNS) that actively regulates CNS homeostasis. A growing body of literature suggests that CP alterations are involved in the pathogenesis of multiple sclerosis (MS) but the underlying mechanisms remain elusive. CPs are enlarged and inflamed in relapsing-remitting (RRMS) but also in clinically isolated syndrome (CIS) and radiologically isolated syndrome (RIS) stages, far beyond MS diagnosis. Increases in the choroid plexus/total intracranial volume (CP/TIV) ratio have been robustly associated with increased lesion load, higher translocator protein (TSPO) uptake in normal-appearing white matter (NAWM) and thalami, as well as with higher annual relapse rate and disability progression in highly active RRMS individuals, but not in progressive MS. The CP/TIV ratio has only slightly been correlated with magnetic resonance imaging (MRI) findings (cortical or whole brain atrophy) and clinical outcomes (EDSS score) in progressive MS. Therefore, we suggest that plexus volumetric assessments should be mainly applied to the early disease stages of MS, whereas it should be taken into consideration with caution in progressive MS. In this review, we attempt to clarify the pathological significance of the temporal CP volume (CPV) changes in MS and highlight the pitfalls and limitations of CP volumetric analysis.
Language	English
Publishing date	2024-04-01
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2721009-1
ISSN	2227-9032
ISSN	2227-9032
DOI	10.3390/healthcare12070768
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Article ; Online: Fitness Characteristics of Jordanian Emergency Medical Technicians.

Al-Yousef, Hani Najeh Mansour / Awada, Wael / Michailidou, Evangelia

Advanced journal of emergency medicine

2019 Volume 3, Issue 4, Page(s) e41

Abstract: Introduction: Emergency medical technicians (EMTs) should be always prepared to deal with the stressful condition of treating patients with serious physical and emotional injuries. Given that EMTs consider safety the first priority, they must pay ... ...

Abstract	Introduction: Emergency medical technicians (EMTs) should be always prepared to deal with the stressful condition of treating patients with serious physical and emotional injuries. Given that EMTs consider safety the first priority, they must pay adequate attention to their own physical well-being and fitness to practice. Objective: The present study was conducted to analyze the fitness of Jordanian EMTs. Method: The present prospective study was conducted to evaluate the well-being of Jordanian paramedics. The survey was designed using Google forms, which were completed by the participants. The data collection tools comprised an already-designed checklist, including items such as age, gender as well as height and weight, which are used for calculating body mass index (BMI). In addition, the presence of chronic diseases such as hypertension, diabetes mellitus, renal failure and cardiorespiratory diseases as well as the history of surgeries and disabilities were investigated. The participants were also asked about their smoking status and other health-related habits. Results: Out of 115 surveys conducted, 7 were discarded owing to loss of information or making completion mistakes. Out of the remaining 108 respondents, 82 (75.9%) were male and below 10% were over the age of 30 years. BMI was over 25 in 40.7% of the respondents, and only 4 (3.7%) had chronic diseases. Moreover, 46 (42.6%) respondents were smokers, and only 30 (27.8%) performed regular exercise. Conclusion: The present findings suggest health problems in a small percentage of the EMTs, potentially due to the appropriate support provided by the employers or university authorities in this regard. The major health problem was overweight and unhealthy lifestyle, including smoking and not doing regular exercise.
Language	English
Publishing date	2019-07-01
Publishing country	Iran
Document type	Journal Article
ISSN	2588-400X
ISSN (online)	2588-400X
DOI	10.22114/ajem.v0i0.160
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Article: Association between History of Prolonged Exclusive Breast-Feeding and the Lung Function Indices in Childhood.

Perikleous, Evanthia P / Fouzas, Sotirios / Michailidou, Magdalena / Patsourou, Anna / Tsalkidis, Dimos / Steiropoulos, Paschalis / Nena, Evangelia / Chatzimichael, Athanasios / Paraskakis, Emmanouil

Children (Basel, Switzerland)

2022 Volume 9, Issue 11

Abstract: Although the propitious effects of breastfeeding on children's health are indisputable, the impact of exclusive breastfeeding on the lung function later in life remains controversial. Our objective was to explore the possible associations between ... ...

Abstract	Although the propitious effects of breastfeeding on children's health are indisputable, the impact of exclusive breastfeeding on the lung function later in life remains controversial. Our objective was to explore the possible associations between breastfeeding and the lung function of children who were exclusively breastfed for an extensive period of time. This was a cross-sectional study of children who were exclusively breastfed for more than 12 months. Demographics and anthropometric data were collected; the body mass index (BMI), % body fat, and % central obesity were calculated; and all the participants underwent standard spirometry with reversibility testing. The relationship between breastfeeding duration and spirometric parameters was assessed by Spearman's correlation and multivariable regression, after adjustment for other confounders. Forty-six children (21 boys), aged 9.2 ± 2.4 years, with a reported breastfeeding duration of 27.5 ± 12.5 months (range 12-60 months) were included; 13% were overweight (none were obese) and 21.7% had central obesity. The average FEV1 was 104.7 ± 10.4% and the average FEF25-75 was 107.9 ± 13.3%. The duration of exclusive breastfeeding was positively correlated with FEF25-75% (r = 0.422,
Language	English
Publishing date	2022-11-08
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2732685-8
ISSN	2227-9067
ISSN	2227-9067
DOI	10.3390/children9111708
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Article ; Online: Fitness Characteristics of Jordanian Emergency Medical Technicians

Hani Najeh Mansour Al Yousef / Wael Awada / Evangelia Michail Michailidou

Advanced Journal of Emergency Medicine (2019)

2019

Abstract	Introduction: Emergency medical technicians (EMTs) should be always prepared to deal with the stressful condition of treating patients with serious physical and emotional injuries. Given that EMTs consider safety the first priority, they must pay adequate attention to their own physical well-being and fitness to practice. Objective: The present study was conducted to analyze the fitness of Jordanian EMTs. Method: The present prospective study was conducted to evaluate the well-being of Jordanian paramedics. The survey was designed using Google forms, which were completed by the participants. The data collection tools comprised an already-designed checklist, including items such as age, gender as well as height and weight, which are used for calculating body mass index (BMI). In addition, the presence of chronic diseases such as hypertension, diabetes mellitus, renal failure and cardiorespiratory diseases as well as the history of surgeries and disabilities were investigated. The participants were also asked about their smoking status and other health-related habits. Results: Out of 115 surveys conducted, 7 were discarded owing to loss of information or making completion mistakes. Out of the remaining 108 respondents, 82 (75.9%) were male and below 10% were over the age of 30 years. BMI was over 25 in 40.7% of the respondents, and only 4 (3.7%) had chronic diseases. Moreover, 46 (42.6%) respondents were smokers, and only 30 (27.8%) performed regular exercise. Conclusion: The present findings suggest health problems in a small percentage of the EMTs, potentially due to the appropriate support provided by the employers or university authorities in this regard. The major health problem was overweight and unhealthy lifestyle, including smoking and not doing regular exercise.
Keywords	Emergency Medical Technicians ; Jordan ; Habits ; Health ; Overweight ; Physical Fitness ; Medicine ; R
Subject code	796
Language	English
Publishing date	2019-07-01T00:00:00Z
Publisher	Tehran University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Outbreaks of SARS-CoV-2 in naturally infected mink farms: Impact, transmission dynamics, genetic patterns, and environmental contamination.

Chaintoutis, Serafeim C / Thomou, Zoi / Mouchtaropoulou, Evangelia / Tsiolas, George / Chassalevris, Taxiarchis / Stylianaki, Ioanna / Lagou, Maria / Michailidou, Sofia / Moutou, Evangelia / Koenen, Jacobus Johannes Hendrik / Dijkshoorn, Jacoba Wilhelmina / Paraskevis, Dimitrios / Poutahidis, Theofilos / Siarkou, Victoria I / Sypsa, Vana / Argiriou, Anagnostis / Fortomaris, Paschalis / Dovas, Chrysostomos I

PLoS pathogens

2021 Volume 17, Issue 9, Page(s) e1009883

Abstract: SARS-CoV-2 infection outbreaks in minks have serious implications associated with animal health and welfare, and public health. In two naturally infected mink farms (A and B) located in Greece, we investigated the outbreaks and assessed parameters ... ...

Abstract	SARS-CoV-2 infection outbreaks in minks have serious implications associated with animal health and welfare, and public health. In two naturally infected mink farms (A and B) located in Greece, we investigated the outbreaks and assessed parameters associated with virus transmission, immunity, pathology, and environmental contamination. Symptoms ranged from anorexia and mild depression to respiratory signs of varying intensity. Although the farms were at different breeding stages, mortality was similarly high (8.4% and 10.0%). The viral strains belonged to lineages B.1.1.218 and B.1.1.305, possessing the mink-specific S-Y453F substitution. Lung histopathology identified necrosis of smooth muscle and connective tissue elements of vascular walls, and vasculitis as the main early key events of the acute SARS-CoV-2-induced broncho-interstitial pneumonia. Molecular investigation in two dead minks indicated a consistently higher (0.3-1.3 log10 RNA copies/g) viral load in organs of the male mink compared to the female. In farm A, the infected farmers were responsible for the significant initial infection of 229 out of 1,000 handled minks, suggesting a very efficient human-to-mink transmission. Subsequent infections across the sheds wherein animals were being housed occurred due to airborne transmission. Based on a R0 of 2.90 and a growth rate equal to 0.293, the generation time was estimated to be 3.6 days, indicative of the massive SARS-CoV-2 dispersal among minks. After the end of the outbreaks, a similar percentage of animals were immune in the two farms (93.0% and 93.3%), preventing further virus transmission whereas, viral RNA was detected in samples collected from shed surfaces and air. Consequently, strict biosecurity is imperative during the occurrence of clinical signs. Environmental viral load monitoring, in conjunction with NGS should be adopted in mink farm surveillance. The minimum proportion of minks that need to be immunized to avoid outbreaks in farms was calculated at 65.5%, which is important for future vaccination campaigns.
MeSH term(s)	Animals ; COVID-19/epidemiology ; COVID-19/genetics ; COVID-19/transmission ; COVID-19/veterinary ; Disease Outbreaks/veterinary ; Environmental Microbiology ; Farms ; Female ; Greece/epidemiology ; Humans ; Male ; Mink/genetics ; Mink/virology ; Occupational Exposure ; Viral Zoonoses/transmission ; Viral Zoonoses/virology
Language	English
Publishing date	2021-09-07
Publishing country	United States
Document type	Journal Article
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7374
ISSN (online)	1553-7374
ISSN	1553-7374
DOI	10.1371/journal.ppat.1009883
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Article ; Online: Mobile App Interventions for Parkinson's Disease, Multiple Sclerosis and Stroke: A Systematic Literature Review.

Triantafyllidis, Andreas / Segkouli, Sofia / Zygouris, Stelios / Michailidou, Christina / Avgerinakis, Konstantinos / Fappa, Evangelia / Vassiliades, Sophia / Bougea, Anastasia / Papagiannakis, Nikos / Katakis, Ioannis / Mathioudis, Evangelos / Sorici, Alexandru / Bajenaru, Lidia / Tageo, Valentina / Camonita, Francesco / Magga-Nteve, Christoniki / Vrochidis, Stefanos / Pedullà, Ludovico / Brichetto, Giampaolo /

Tsakanikas, Panagiotis / Votis, Konstantinos / Tzovaras, Dimitrios

Sensors (Basel, Switzerland)

2023 Volume 23, Issue 7

Abstract: Central nervous system diseases (CNSDs) lead to significant disability worldwide. Mobile app interventions have recently shown the potential to facilitate monitoring and medical management of patients with CNSDs. In this direction, the characteristics of ...

Abstract	Central nervous system diseases (CNSDs) lead to significant disability worldwide. Mobile app interventions have recently shown the potential to facilitate monitoring and medical management of patients with CNSDs. In this direction, the characteristics of the mobile apps used in research studies and their level of clinical effectiveness need to be explored in order to advance the multidisciplinary research required in the field of mobile app interventions for CNSDs. A systematic review of mobile app interventions for three major CNSDs, i.e., Parkinson's disease (PD), multiple sclerosis (MS), and stroke, which impose significant burden on people and health care systems around the globe, is presented. A literature search in the bibliographic databases of PubMed and Scopus was performed. Identified studies were assessed in terms of quality, and synthesized according to target disease, mobile app characteristics, study design and outcomes. Overall, 21 studies were included in the review. A total of 3 studies targeted PD (14%), 4 studies targeted MS (19%), and 14 studies targeted stroke (67%). Most studies presented a weak-to-moderate methodological quality. Study samples were small, with 15 studies (71%) including less than 50 participants, and only 4 studies (19%) reporting a study duration of 6 months or more. The majority of the mobile apps focused on exercise and physical rehabilitation. In total, 16 studies (76%) reported positive outcomes related to physical activity and motor function, cognition, quality of life, and education, whereas 5 studies (24%) clearly reported no difference compared to usual care. Mobile app interventions are promising to improve outcomes concerning patient's physical activity, motor ability, cognition, quality of life and education for patients with PD, MS, and Stroke. However, rigorous studies are required to demonstrate robust evidence of their clinical effectiveness.
MeSH term(s)	Humans ; Mobile Applications ; Quality of Life ; Multiple Sclerosis/therapy ; Parkinson Disease/therapy ; Stroke/therapy
Language	English
Publishing date	2023-03-23
Publishing country	Switzerland
Document type	Systematic Review ; Journal Article ; Review
ZDB-ID	2052857-7
ISSN	1424-8220 ; 1424-8220
ISSN (online)	1424-8220
ISSN	1424-8220
DOI	10.3390/s23073396
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Article: Enhanced recovery after surgery in liver transplantation: Challenges and feasibility.

Katsanos, Georgios / Karakasi, Konstantina-Eleni / Antoniadis, Nikolaos / Vasileiadou, Stella / Kofinas, Athanasios / Morsi-Yeroyannis, Antonios / Michailidou, Evangelia / Goulis, Ioannis / Sinakos, Emmanouil / Giouleme, Olga / Oikonomou, Ilias Marios / Evlavis, George / Tsakiris, Georgios / Massa, Eleni / Mouloudi, Eleni / Tsoulfas, Georgios

World journal of transplantation

2022 Volume 12, Issue 7, Page(s) 195–203

Abstract: Background: Enhanced recovery after surgery (ERAS) started a revolution that changed age-old surgical stereotypical practices regarding the overall management of the surgical patient. In the last decade, ERAS has gained significant acceptance in the ... ...

Abstract	Background: Enhanced recovery after surgery (ERAS) started a revolution that changed age-old surgical stereotypical practices regarding the overall management of the surgical patient. In the last decade, ERAS has gained significant acceptance in the community of general surgery, in addition to several other surgical specialties, as the evidence of its advantages continues to grow. One of the last remaining fields, given its significant complexity and intricate nature, is liver transplantation (LT). Aim: To investigate the existing efforts at implementing ERAS in LT. Methods: We conducted a systematic review of the existing studies that evaluate ERAS in orthotopic LT, with a multimodal approach and focusing on measurable clinical primary endpoints, namely length of hospital stay. Results: All studies demonstrated a considerable decrease in length of hospital stay, with no readmission or negative impact of the ERAS protocol applied to the postoperative course. Conclusions: ERAS is a well-validated multimodal approach for almost all types of surgical procedures, and its future in selected LT patients seems promising, as the preliminary results advocate for the safety and efficacy of ERAS in the field of LT.
Language	English
Publishing date	2022-08-27
Publishing country	United States
Document type	Journal Article
ISSN	2220-3230
ISSN	2220-3230
DOI	10.5500/wjt.v12.i7.195
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