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  1. Article ; Online: Measurement of the normal contralateral hip flexion angle and posterior pelvic tilt angle using a pelvic guide pin in total hip arthroplasty.

    Nakanishi, Takumi / Kato, Michitaka / Kizawa, Lisa / Warashina, Hideki / Mitamura, Shingo / Kataoka, Akito

    Clinical biomechanics (Bristol, Avon)

    2023  Volume 106, Page(s) 105982

    Abstract: Background: We aimed to measure the posterior pelvic tilt angle at maximum hip flexion and hip flexion range of motion at the femoroacetabular joint using a pelvic guide pin and to examine the difference in flexion range of motion when determined by a ... ...

    Abstract Background: We aimed to measure the posterior pelvic tilt angle at maximum hip flexion and hip flexion range of motion at the femoroacetabular joint using a pelvic guide pin and to examine the difference in flexion range of motion when determined by a physical therapist and under anesthesia.
    Methods: Data of 83 consecutive patients undergoing primary unilateral total hip arthroplasty were assessed. Using a pin inserted into the iliac crest to define the cup placement angle under anesthesia before and after total hip arthroplasty, the posterior pelvic tilt angle was determined as the change in pin tilt from the supine position to maximum hip flexion. Flexion range of motion under anesthesia was calculated as the difference in the angle between the trunk and thigh at maximum flexion and the posterior pelvic tilt angle. Flexion range of motion with a fixed pelvis measured preoperatively by a physical therapist was compared to that under anesthesia. A goniometer was used for all measurements and the number of measurements was one.
    Findings: Mean posterior pelvic tilt angle of the pin inserted into the pelvis under anesthesia was 15.8° ± 5.3° (3°-26°) preoperatively and 12.1° ± 4.9° (3°-26°) postoperatively. Mean flexion range of motion under anesthesia was 109.4° ± 6.9° (88°-126°) and that measured by a physical therapist was 101.1° ± 8.2° (80°-120°); the difference was significant (9.7°; p < 0.01).
    Interpretation: These results highlight the difficulty in accurately determining hip flexion angles without the use of special devices and may help surgeons and physical therapists recognize and address this issue.
    MeSH term(s) Humans ; Arthroplasty, Replacement, Hip/methods ; Hip Joint/diagnostic imaging ; Hip Joint/surgery ; Pelvis/diagnostic imaging ; Pelvis/surgery ; Posture ; Thigh ; Range of Motion, Articular
    Language English
    Publishing date 2023-05-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 632747-3
    ISSN 1879-1271 ; 0268-0033
    ISSN (online) 1879-1271
    ISSN 0268-0033
    DOI 10.1016/j.clinbiomech.2023.105982
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Scaffold-free 3D bio-printed human liver tissue stably maintains metabolic functions useful for drug discovery.

    Kizawa, Hideki / Nagao, Eri / Shimamura, Mitsuru / Zhang, Guangyuan / Torii, Hitoshi

    Biochemistry and biophysics reports

    2017  Volume 10, Page(s) 186–191

    Abstract: The liver plays a central role in metabolism. Although many studies have ... ...

    Abstract The liver plays a central role in metabolism. Although many studies have described
    Language English
    Publishing date 2017-04-14
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2831046-9
    ISSN 2405-5808
    ISSN 2405-5808
    DOI 10.1016/j.bbrep.2017.04.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Scaffold-free 3D bio-printed human liver tissue stably maintains metabolic functions useful for drug discovery

    Hideki Kizawa / Eri Nagao / Mitsuru Shimamura / Guangyuan Zhang / Hitoshi Torii

    Biochemistry and Biophysics Reports, Vol 10, Iss C, Pp 186-

    2017  Volume 191

    Abstract: The liver plays a central role in metabolism. Although many studies have described in vitro liver models for drug discovery, to date, no model has been described that can stably maintain liver function. Here, we used a unique, scaffold-free 3D bio- ... ...

    Abstract The liver plays a central role in metabolism. Although many studies have described in vitro liver models for drug discovery, to date, no model has been described that can stably maintain liver function. Here, we used a unique, scaffold-free 3D bio-printing technology to construct a small portion of liver tissue that could stably maintain drug, glucose, and lipid metabolism, in addition to bile acid secretion. This bio-printed normal human liver tissue maintained expression of several kinds of hepatic drug transporters and metabolic enzymes that functioned for several weeks. The bio-printed liver tissue displayed glucose production via cAMP/protein kinase A signaling, which could be suppressed with insulin. Bile acid secretion was also observed from the printed liver tissue, and it accumulated in the culture medium over time. We observed both bile duct and sinusoid-like structures in the bio-printed liver tissue, which suggested that bile acid secretion occurred via a sinusoid-hepatocyte-bile duct route. These results demonstrated that our bio-printed liver tissue was unique, because it exerted diverse liver metabolic functions for several weeks. In future, we expect our bio-printed liver tissue to be applied to developing new models that can be used to improve preclinical predictions of long-term toxicity in humans, generate novel targets for metabolic liver disease, and evaluate biliary excretion in drug development.
    Keywords Scaffold-free ; 3D ; Bio-printing ; Liver ; Drug discovery ; Metabolism ; Biology (General) ; QH301-705.5 ; Biochemistry ; QD415-436
    Language English
    Publishing date 2017-07-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Development of gapmer antisense oligonucleotide with deoxyribonucleic guanidine (DNG) modifications.

    Kojima, Naoshi / Shrestha, Ajaya R / Akisawa, Takuya / Piao, Haishun / Kizawa, Hideki / Ohmiya, Yoshihiro / Kurita, Ryoji

    Nucleosides, nucleotides & nucleic acids

    2019  Volume 39, Issue 1-3, Page(s) 258–269

    Abstract: The properties of gapmer antisense oligonucleotide (ASO) flanked by deoxyribonucleic guanidine (DNG) were investigated for the potential application in antisense technology. DNG is a unique nucleotide analog which has a positively charged internucleotide ...

    Abstract The properties of gapmer antisense oligonucleotide (ASO) flanked by deoxyribonucleic guanidine (DNG) were investigated for the potential application in antisense technology. DNG is a unique nucleotide analog which has a positively charged internucleotide guanidinium linkage instead of negatively charged phosphodiester backbone linkage. We prepared a gapmer ASO containing DNG units at both wings of the sequence and compared its properties with 2',4'-BNA/LNA gapmer ASOs with phosphorothioate (PS) backbone. Although DNG gapmer showed no stabilizing effect on the duplex formation with target RNA, the DNG modification was found to be tolerant to exonuclease digestion. Furthermore, DNG gapmer can induce RNase H-mediated cleavage of target RNA molecule, a requisite property for the antisense strategy. Therefore, the DNG gapmer developed in this study could be an interesting and useful candidate for the development of potent ASOs.
    MeSH term(s) Base Sequence ; Chemistry Techniques, Synthetic ; DNA/chemistry ; DNA/genetics ; Guanidines/chemistry ; Humans ; Molecular Structure ; Oligonucleotides, Antisense/chemical synthesis ; Oligonucleotides, Antisense/chemistry ; Oligonucleotides, Antisense/genetics ; RNA/chemistry ; RNA/genetics ; RNA Cleavage ; Thermodynamics ; Transition Temperature
    Chemical Substances Guanidines ; Oligonucleotides, Antisense ; RNA (63231-63-0) ; DNA (9007-49-2)
    Language English
    Publishing date 2019-09-26
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2008956-9
    ISSN 1532-2335 ; 1525-7770
    ISSN (online) 1532-2335
    ISSN 1525-7770
    DOI 10.1080/15257770.2019.1668563
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: High-resolution SNP map of ASPN, a susceptibility gene for osteoarthritis.

    Iida, Aritoshi / Kizawa, Hideki / Nakamura, Yusuke / Ikegawa, Shiro

    Journal of human genetics

    2005  Volume 51, Issue 2, Page(s) 151–154

    Abstract: Osteoarthritis (OA) is a very common bone and joint disease characterized by breakdown of cartilage in the joint. We recently found that an aspartic-acid repeat polymorphism of the asporin gene (ASPN) on chromosome 9 is associated with susceptibility to ... ...

    Abstract Osteoarthritis (OA) is a very common bone and joint disease characterized by breakdown of cartilage in the joint. We recently found that an aspartic-acid repeat polymorphism of the asporin gene (ASPN) on chromosome 9 is associated with susceptibility to OA in Japanese. We provide here a high-resolution single nucleotide polymorphism (SNP) map within a 33.4-kb genomic region containing ASPN. A total of 19 SNPs were isolated from the region by systematic screening using 48 Japanese patients with OA: 7 SNPs in the 5' flanking region, 8 in introns, and 4 in the 3' untranslated region. Nine SNPs were novel. This high-resolution SNP map will be a useful resource for analyzing genes associated with OA and other bone and joint diseases.
    MeSH term(s) Carrier Proteins/genetics ; Chromosomes, Human, Pair 9/genetics ; DNA Primers ; Extracellular Matrix Proteins ; Gene Components ; Gene Frequency ; Genetic Predisposition to Disease ; Haplotypes/genetics ; Humans ; Japan ; Osteoarthritis/genetics ; Polymorphism, Single Nucleotide ; Sequence Analysis, DNA
    Chemical Substances ASPN protein, human ; Carrier Proteins ; DNA Primers ; Extracellular Matrix Proteins
    Language English
    Publishing date 2005-11-26
    Publishing country England
    Document type Comparative Study ; Journal Article
    ZDB-ID 1425192-9
    ISSN 1435-232X ; 1434-5161
    ISSN (online) 1435-232X
    ISSN 1434-5161
    DOI 10.1007/s10038-005-0337-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Equivalent osteoblastic differentiation function of human mesenchymal stem cells from rheumatoid arthritis in comparison with osteoarthritis.

    Morimoto, Daiki / Kuroda, Shoko / Kizawa, Takuji / Nomura, Koji / Higuchi, Chikahisa / Yoshikawa, Hideki / Tomita, Tetsuya

    Rheumatology (Oxford, England)

    2009  Volume 48, Issue 6, Page(s) 643–649

    Abstract: Objective: To evaluate the osteoblastic differentiation of human mesenchymal stem cells (hMSCs) in patients with RA.: Methods: Heparinized bone marrow aspirate was obtained from patients with OA and RA. Mononuclear cells were cultured for 2 weeks and ...

    Abstract Objective: To evaluate the osteoblastic differentiation of human mesenchymal stem cells (hMSCs) in patients with RA.
    Methods: Heparinized bone marrow aspirate was obtained from patients with OA and RA. Mononuclear cells were cultured for 2 weeks and a colony-forming assay was performed. The phenotype of cells was analysed by flow cytometry. Passage 2 cells were cultured with beta-glycerophosphate (bGP) in the control group and bGP, ascorbic acid and dexamethasone in the differentiation group. After 2 weeks, ALP staining and activity were performed. After 3 weeks, Alizarin Red S assay was performed. Total RNA was extracted from cells cultured for 2 and 3 weeks. Gene expression of bone formation factor was examined by real-time PCR.
    Results: The phenotype of cells was identical in both OA and RA and the content was thought to be hMSCs. The results of ALP activity and Alizarin Red S assay showed higher levels in the differentiation group for both OA and RA samples compared with the control group. The results of a colony-forming assay were identical in both OA and RA samples. Gene expression in the differentiation group was higher than in the control group in both OA and RA samples. There was no significant difference between OA and RA samples in all experiments.
    Conclusion: The function of osteoblastic differentiation of hMSCs is similar between OA and RA.
    MeSH term(s) Aged ; Aged, 80 and over ; Alkaline Phosphatase/analysis ; Arthritis, Rheumatoid/pathology ; Biomarkers/analysis ; Case-Control Studies ; Cell Culture Techniques ; Cell Differentiation ; Cells, Cultured ; Colony-Forming Units Assay ; Female ; Flow Cytometry ; Gene Expression ; Humans ; Mesenchymal Stromal Cells/pathology ; Middle Aged ; Osteoarthritis/pathology ; Osteoblasts/pathology ; Osteocalcin/analysis ; Osteopontin/analysis ; Phenotype ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Staining and Labeling ; Statistics, Nonparametric
    Chemical Substances Biomarkers ; Osteocalcin (104982-03-8) ; Osteopontin (106441-73-0) ; Alkaline Phosphatase (EC 3.1.3.1)
    Language English
    Publishing date 2009-06
    Publishing country England
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1464822-2
    ISSN 1462-0332 ; 1462-0324
    ISSN (online) 1462-0332
    ISSN 1462-0324
    DOI 10.1093/rheumatology/kep044
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: [Hypothyroid myopathy caused by interferon-alpha therapy for chronic hepatitis C].

    Nagane, Yuriko / Utsugisawa, Kimiaki / Kizawa, Hideki / Kondoh, Ryushi / Terayama, Yasuo

    Rinsho shinkeigaku = Clinical neurology

    2005  Volume 45, Issue 6, Page(s) 441–444

    Abstract: We reported a rare case with hypothyroid myopathy after interferon-alpha (IFN-alpha) therapy. A 59-year-old man complained of his weakness in proximal part of upper and lower extremities which started at 1 month and progressed during 6 months after IFN- ... ...

    Abstract We reported a rare case with hypothyroid myopathy after interferon-alpha (IFN-alpha) therapy. A 59-year-old man complained of his weakness in proximal part of upper and lower extremities which started at 1 month and progressed during 6 months after IFN-alpha therapy for chronic hepatitis C, but he did not complain of any other symptoms. Blood chemistry showed an elevated level of CK (1,843 IU/l; normal range 43-170 IU/l) and increased myoglobin (250 ng/ml; normal range < 60 ng/ml). Thyroid function tests revealed an elevated level of TSH (148.7 microIU/ml; normal range, 0.4-4.1 microIU/ml), and decreased levels of free T3 (0.56 pg/ml; normal range, 2.27-3.90 pg/ml) and free T4 (0.24 ng/ml; normal range, 0.95-1.74 ng/ml). Blocking type TSH receptor antibody titer was elevated (75.4%; normal range < 15%) while other types of antithyroid antibody were not detected in his serum. Muscle biopsy from his quadriceps femoris muscle showed non-specific mild myopathic changes. His weakness was completely ameliorated and serum CK levels were normalized by thyroid hormone administration alone, confirming the diagnosis of hypothyroid myopathy. However, even after total amelioration of his hypothyroidism, blocking type TSH receptor antibody titer remained elevated. These findings may suggest that IFN-alpha fostered an autoreactivity arising from HCV infection to cause the autoimmune thyroid disease.
    MeSH term(s) Antiviral Agents/administration & dosage ; Antiviral Agents/adverse effects ; Hepatitis C, Chronic/drug therapy ; Humans ; Hypothyroidism/chemically induced ; Interferon-alpha/administration & dosage ; Interferon-alpha/adverse effects ; Male ; Middle Aged ; Muscular Diseases/chemically induced ; Recombinant Proteins ; Ribavirin/administration & dosage
    Chemical Substances Antiviral Agents ; Interferon-alpha ; Recombinant Proteins ; interferon alfa-2b (43K1W2T1M6) ; Ribavirin (49717AWG6K)
    Language Japanese
    Publishing date 2005-06
    Publishing country Japan
    Document type Case Reports ; Journal Article
    ZDB-ID 604200-4
    ISSN 1882-0654 ; 0009-918X
    ISSN (online) 1882-0654
    ISSN 0009-918X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Mechanisms for asporin function and regulation in articular cartilage.

    Nakajima, Masahiro / Kizawa, Hideki / Saitoh, Masao / Kou, Ikuyo / Miyazono, Kohei / Ikegawa, Shiro

    The Journal of biological chemistry

    2007  Volume 282, Issue 44, Page(s) 32185–32192

    Abstract: ... the cartilage extracellular matrix protein asporin and OA (Kizawa, H., Kou, I., Iida, A., Sudo, A., Miyamoto, Y ...

    Abstract Osteoarthritis (OA), the most prevalent form of skeletal disease, represents a leading cause of disability following middle age. OA is characterized by the loss of articular cartilage; however, the details of its etiology and pathogenesis remain unclear. Recently, we demonstrated a genetic association between the cartilage extracellular matrix protein asporin and OA (Kizawa, H., Kou, I., Iida, A., Sudo, A., Miyamoto, Y., Fukuda, A., Mabuchi, A., Kotani, A., Kawakami, A., Yamamoto, S., Uchida, A., Nakamura, K., Notoya, K., Nakamura, Y., and Ikegawa, S. (2005) Nat. Genet. 37, 138-144). Furthermore, we showed that asporin binds to transforming growth factor-beta (TGF-beta), a key cytokine in OA pathogenesis, and inhibits TGF-beta-induced chondrogenesis. To date, functional data for asporin have come primarily from mouse cell culture models of developing cartilage rather than from human articular cartilage cells, in which OA occurs. Here, we describe mechanisms for asporin function and regulation in human articular cartilage. Asporin blocks chondrogenesis and inhibits TGF-beta1-induced expression of matrix genes and the resulting chondrocyte phenotypes. Small interfering RNA-mediated knockdown of asporin increases the expression of cartilage marker genes and TGF-beta1; in turn, TGF-beta1 stimulates asporin expression in articular cartilage cells, suggesting that asporin and TGF-beta1 form a regulatory feedback loop. Asporin inhibits TGF-beta/Smad signaling upstream of TGF-beta type I receptor activation in vivo by co-localizing with TGF-beta1 on the cell surface and blocking its interaction with the TGF-beta type II receptor. Our results provide a basis for elucidating the role of asporin in the molecular pathogenesis of OA.
    MeSH term(s) Cartilage, Articular/metabolism ; Cell Differentiation ; Cell Line ; Extracellular Matrix Proteins/metabolism ; Humans ; Osteoarthritis, Knee/metabolism ; Protein Isoforms/metabolism ; Transforming Growth Factor beta1/metabolism
    Chemical Substances ASPN protein, human ; Extracellular Matrix Proteins ; Protein Isoforms ; Transforming Growth Factor beta1
    Language English
    Publishing date 2007-09-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    DOI 10.1074/jbc.M700522200
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Proton magnetic resonance spectroscopy and diffusion-weighted imaging of tumefactive demyelinating plaque.

    Masu, Kazuki / Beppu, Takaaki / Fujiwara, Shunrou / Kizawa, Hideki / Kashimura, Hiroshi / Kurose, Akira / Ogasawara, Kuniaki / Sasaki, Makoto

    Neurologia medico-chirurgica

    2009  Volume 49, Issue 9, Page(s) 430–433

    Abstract: Proton magnetic resonance spectroscopy, diffusion-weighted axonography, and diffusion tensor tractography in a patient with tumefactive demyelination plaque (TDP) were evaluated for differential diagnosis from glioblastoma. The findings of glutamate and ... ...

    Abstract Proton magnetic resonance spectroscopy, diffusion-weighted axonography, and diffusion tensor tractography in a patient with tumefactive demyelination plaque (TDP) were evaluated for differential diagnosis from glioblastoma. The findings of glutamate and glutamine elevations on magnetic resonance spectroscopy and apparent tracts within the lesion on axonography and tractography were unlikely to represent glioblastoma, and were thus useful for the preoperative diagnosis of TDP.
    MeSH term(s) Biomarkers, Tumor/analysis ; Brain/metabolism ; Brain/pathology ; Brain/physiopathology ; Brain Neoplasms/diagnosis ; Chemotaxis, Leukocyte ; Demyelinating Diseases/diagnosis ; Demyelinating Diseases/physiopathology ; Diagnosis, Differential ; Diagnostic Errors/prevention & control ; Diffusion Magnetic Resonance Imaging ; Diffusion Tensor Imaging ; Glioblastoma/diagnosis ; Humans ; Magnetic Resonance Spectroscopy ; Male ; Middle Aged ; Nerve Fibers, Myelinated/pathology ; Predictive Value of Tests ; Putamen/pathology ; Pyramidal Tracts/pathology ; Sensitivity and Specificity
    Chemical Substances Biomarkers, Tumor
    Language English
    Publishing date 2009-09-24
    Publishing country Japan
    Document type Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 604061-5
    ISSN 1349-8029 ; 0470-8105
    ISSN (online) 1349-8029
    ISSN 0470-8105
    DOI 10.2176/nmc.49.430
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Mechanisms for Asporin Function and Regulation in Articular Cartilage

    Nakajima, Masahiro / Kizawa, Hideki / Saitoh, Masao / Kou, Ikuyo / Miyazono, Kohei / Ikegawa, Shiro

    Journal of biological chemistry. 2007 Nov. 2, v. 282, no. 44

    2007  

    Abstract: ... the cartilage extracellular matrix protein asporin and OA (Kizawa, H., Kou, I., Iida, A., Sudo, A., Miyamoto, Y ...

    Abstract Osteoarthritis (OA), the most prevalent form of skeletal disease, represents a leading cause of disability following middle age. OA is characterized by the loss of articular cartilage; however, the details of its etiology and pathogenesis remain unclear. Recently, we demonstrated a genetic association between the cartilage extracellular matrix protein asporin and OA (Kizawa, H., Kou, I., Iida, A., Sudo, A., Miyamoto, Y., Fukuda, A., Mabuchi, A., Kotani, A., Kawakami, A., Yamamoto, S., Uchida, A., Nakamura, K., Notoya, K., Nakamura, Y., and Ikegawa, S. (2005) Nat. Genet. 37, 138-144). Furthermore, we showed that asporin binds to transforming growth factor-β (TGF-β), a key cytokine in OA pathogenesis, and inhibits TGF-β-induced chondrogenesis. To date, functional data for asporin have come primarily from mouse cell culture models of developing cartilage rather than from human articular cartilage cells, in which OA occurs. Here, we describe mechanisms for asporin function and regulation in human articular cartilage. Asporin blocks chondrogenesis and inhibits TGF-β1-induced expression of matrix genes and the resulting chondrocyte phenotypes. Small interfering RNA-mediated knockdown of asporin increases the expression of cartilage marker genes and TGF-β1; in turn, TGF-β1 stimulates asporin expression in articular cartilage cells, suggesting that asporin and TGF-β1 form a regulatory feedback loop. Asporin inhibits TGF-β/Smad signaling upstream of TGF-β type I receptor activation in vivo by co-localizing with TGF-β1 on the cell surface and blocking its interaction with the TGF-β type II receptor. Our results provide a basis for elucidating the role of asporin in the molecular pathogenesis of OA.
    Language English
    Dates of publication 2007-1102
    Size p. 32185-32192.
    Publishing place American Society for Biochemistry and Molecular Biology
    Document type Article
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    Database NAL-Catalogue (AGRICOLA)

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