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  1. Article: Antibacterial activity of Eurycoma longifolia Jack. A Malaysian medicinal plant.

    Farouk, Abd-Elaziem / Benafri, Asma

    Saudi medical journal

    2007  Volume 28, Issue 9, Page(s) 1422–1424

    Abstract: Objective: To evaluate methanolic, ethanolic, acetone and aqueous extracts from different parts of Eurycoma longifolia (E. longifolia) (leave, stem, and root) for antibacterial activity against Gram-positive and Gram-negative bacteria and to utilize the ...

    Abstract Objective: To evaluate methanolic, ethanolic, acetone and aqueous extracts from different parts of Eurycoma longifolia (E. longifolia) (leave, stem, and root) for antibacterial activity against Gram-positive and Gram-negative bacteria and to utilize the leaves and stem parts rather than the root, which is already used for male sexual enhancement in Malaysia.
    Methods: The study took place in the Laboratory of Molecular Biology of Biotechnology Engineering Department, Malaysia between January 2005 and June 2006. Methanolic, ethanolic, acetone and aqueous extracts of leaves, stems and roots of E. longifolia were investigated for their antibacterial properties using Agar-well diffusion method.
    Results: The alcoholic and acetone extracts of the leaves and stem extracts were active on both Gram-positive and Gram-negative bacteria except against 2 strains of Gram-negative bacteria (Escherichia coli and Salmonella typhi). The root extracts had no antibacterial activity against Gram-positive and Gram-negative bacteria tested. Aqueous leaves extract showed antibacterial activity against Staphylococcus aureus and Serratia marscesens.
    Conclusion: The alcoholic and acetone extracts from leaves and stems of E. longifolia contain potent antibacterial agent(s). This plant can serve as a potential source of antibacterial compounds.
    MeSH term(s) Eurycoma ; Gram-Negative Bacteria/drug effects ; Gram-Negative Bacteria/growth & development ; Gram-Positive Bacteria/drug effects ; Gram-Positive Bacteria/growth & development ; Microbial Sensitivity Tests ; Plant Extracts/pharmacology ; Plant Leaves ; Plant Roots ; Plant Stems
    Chemical Substances Plant Extracts
    Language English
    Publishing date 2007-09
    Publishing country Saudi Arabia
    Document type Journal Article
    ZDB-ID 392302-2
    ISSN 1658-3175 ; 0379-5284
    ISSN (online) 1658-3175
    ISSN 0379-5284
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 2330: Show issues Location:
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    ab Jg. 2022: Lesesaal (EG)
    Zs.MB 6: Show issues

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  2. Article ; Online: R unning Head

    Abd - ElAziem Farouk / Anis Shobirin Meor Hussin / Ralf Greiner / Shareef Mohideen Ismail / Mohamed Faizal Batcha / Hamadah Mohd Nur Lubis

    ASEAN Journal on Science and Technology for Development, Vol 27, Iss

    Novel Recombinant Phytate - d egrading Enzyme Production Statistical Optimization of the Induction of Phytase Production by Arabinose in a recombinant E. coli using Response Surface Methodology

    2017  Volume 1

    Abstract: The production of phytase in a recombinant E.coliusing thepBAD expression system wasoptimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentrationin the cultivation ... ...

    Abstract The production of phytase in a recombinant E.coliusing thepBAD expression system wasoptimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentrationin the cultivation media and the incubation temperature were optimized in order to maximize phytase productionusing 23 central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among thesefactors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature. OPtim al conditionsfor phytase production were determinedto be100 μg/ml ampicilin, 0.2 % arabinose and an incubation temperature of37ºC.The production of phytase in the recombinant E. coliwas scaledup to 100ml and 1000ml.
    Keywords recombinant phytase ; statistical optimization ; cultivation conditions ; Technology (General) ; T1-995 ; Science (General) ; Q1-390
    Subject code 660
    Language English
    Publishing date 2017-11-01T00:00:00Z
    Publisher Universitas Gadjah Mada
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: myo-inositol phosphate isomers generated by the action of a phytase from a malaysian waste-water bacterium.

    Greiner, Ralf / Farouk, Abd-Elaziem / Carlsson, Nils-Gunnar / Konietzny, Ursula

    The protein journal

    2007  Volume 26, Issue 8, Page(s) 577–584

    Abstract: Using a combination of High-Performance Ion Chromatography analysis and kinetic studies, the pathway of myo-inositol hexakisphosphate dephosphorylation by a phytase from a Malaysian waste-water bacterium was established. The data demonstrate that the ... ...

    Abstract Using a combination of High-Performance Ion Chromatography analysis and kinetic studies, the pathway of myo-inositol hexakisphosphate dephosphorylation by a phytase from a Malaysian waste-water bacterium was established. The data demonstrate that the phytase preferably dephosphorylates myo-inositol hexakisphosphate in a stereospecific way by sequential removal of phosphate groups via D-I(1,2,3,4,5)P(5), D-I(2,3,4,5)P(4), D-I(2,3,4)P(3), D-I(2,3)P(2) to finally I(2)P. It was estimated that more than 90% of phytate hydrolysis occurs via D-I(1,2,3,4,5)P(5). Thus, the phytase from the Malaysian waste-water bacterium has to be considered a 6-phytase (E.C. 3.1.3.26). A second pathway of minor importance could be proposed which is in accordance with the results obtained from analysis of the dephosphorylation products formed by the action of the phytase under investigation on myo-inositol hexakisphosphate. It proceeds via D/L-I(1,2,4,5,6)P(5), D/L-I(1,2,4,5)P(4), D/L-I(1,2,4)P(3), D/L-I(2,4)P(2) to finally I(2)P.
    MeSH term(s) 6-Phytase/isolation & purification ; 6-Phytase/metabolism ; Bacteria/enzymology ; Chromatography, High Pressure Liquid ; Hydrolysis ; Isomerism ; Kinetics ; Molecular Conformation ; Phosphorylation ; Phytic Acid/metabolism ; Secale/enzymology ; Sewage/microbiology ; Water Microbiology
    Chemical Substances Sewage ; Phytic Acid (7IGF0S7R8I) ; 6-Phytase (EC 3.1.3.26)
    Language English
    Publishing date 2007-12
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2143071-8
    ISSN 1875-8355 ; 1573-4943 ; 1572-3887
    ISSN (online) 1875-8355 ; 1573-4943
    ISSN 1572-3887
    DOI 10.1007/s10930-007-9100-5
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  4. Article ; Online: Comparison of Alcohol Free GSH Production by Ultrasonic and Homogenizing Method

    Mohamad Ramlan Mohamed Salleh / Parveen Jamal / Abd. Elaziem Farouk Gad / Yumi Zuhanis Has-Yun Hashim / Haslinda Hasim

    Iranian Journal of Chemistry & Chemical Engineering, Vol 27, Iss 4, Pp 107-

    2008  Volume 113

    Abstract: Homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free GSH) from baker’s yeast. The study was done to determine the effects of specific parameters of the ... ...

    Abstract Homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free GSH) from baker’s yeast. The study was done to determine the effects of specific parameters of the methods and the pH of the medium on the production of the GSH. The cell disruptions were run at a wide range of those parameters at the suitable common parameters of the methods. The disrupted cells suspension was then centrifuged at 12,000 rpm for 20 min and the GSH content in the supernatant was analyzed by taking the absorbance (A) value of the solution by spectrophotometer at 412 nm. The results obtained showed that the production of GSH was affected by the adjusted specific parameters of each method and the yield of production by the homogenizing and ultrasonic method was found 3.3 and 4.1 times, respectively, better than the yield of production by the unadjusted parameters. Ultrasonic method which produced 23.2 mg/L product was found 1.3 times better than the homogenizing method (18.5 mg/L) in producing the GSH. The result obtained from the lab scale study of the above mentioned mechanical methods showed that the implementation of them in the large scale production of the alcohol free GSH is possible.
    Keywords alcohol free gsh ; ultrasonic method ; homogenizing method ; baker’s yeast ; cells disruption ; specific parameters ; Chemical engineering ; TP155-156 ; Chemistry ; QD1-999
    Subject code 670
    Language English
    Publishing date 2008-12-01T00:00:00Z
    Publisher Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Isolation of GSH from Baker Yeast by Homogenizer Method

    Mohamad Ramlan Bin Mohamed Salleh / Abd. Elaziem Farouk / Rohani Binti Salleh / Haslinda Binti Hasim

    Pakistan Journal of Biological Sciences, Vol 7, Iss 9, Pp 1636-

    2004  Volume 1639

    Abstract: The purpose of this study was to determine the effect of operating parameters such as the yeast concentration, processing temperature and isolation time on the isolation of GSH using Homogenizer Method. The disrupted cells solution was centrifuged at 12, ... ...

    Abstract The purpose of this study was to determine the effect of operating parameters such as the yeast concentration, processing temperature and isolation time on the isolation of GSH using Homogenizer Method. The disrupted cells solution was centrifuged at 12,000 rpm for 20 min and the GSH content in the supernatant was analyzed by taking the optical density (OD) value of the solution using Spectrophotometer at 412 nm. Yeast solution with the concentration of 3, 6, 9 and 12 wt.% was disrupted at their original temperature for 30 sec to study the effect of yeast concentration. The result obtained shows that the optimum value of yeast concentration is 9 wt.% where 10.98 μmol mL -1 GSH was isolated. To study the effect of processing temperature, isolation process of the 9 wt.% yeast solution was done at various values of temperature such as 19, 22, 25, 28, 30 and 40�C for 30 sec. The results indicated that, the optimum GSH isolation temperature is 22�C with the isolation of 17.93 μmol mL -1 GSH. Then, to study the effect of isolation time, the isolation process of sample with the concentration of 9 wt.% was done at 22�C for 15, 30, 45 and 60 sec. The result indicated that the optimum isolation time for the Ultrasonic process is 30 sec.
    Keywords Biology (General) ; QH301-705.5 ; Science ; Q ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
    Subject code 660
    Language English
    Publishing date 2004-01-01T00:00:00Z
    Publisher Asian Network for Scientific Information
    Document type Article ; Online
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  6. Article: New quinoline-based triazole hybrid analogs as effective inhibitors of α-amylase and α-glucosidase: Preparation,

    Khan, Yousaf / Iqbal, Shahid / Shah, Mazloom / Maalik, Aneela / Hussain, Rafaqat / Khan, Shoaib / Khan, Imran / Pashameah, Rami Adel / Alzahrani, Eman / Farouk, Abd-ElAziem / Alahmdi, Mohammed Issa / Abd-Rabboh, Hisham S M

    Frontiers in chemistry

    2022  Volume 10, Page(s) 995820

    Abstract: The 7-quinolinyl-bearing triazole analogs were ... ...

    Abstract The 7-quinolinyl-bearing triazole analogs were synthesized
    Language English
    Publishing date 2022-09-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2711776-5
    ISSN 2296-2646
    ISSN 2296-2646
    DOI 10.3389/fchem.2022.995820
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  7. Article ; Online: Improvement in Optoelectronic Properties of Bismuth Sulphide Thin Films by Chromium Incorporation at the Orthorhombic Crystal Lattice for Photovoltaic Applications.

    Fazal, Tanzeela / Iqbal, Shahid / Shah, Mazloom / Ismail, Bushra / Shaheen, Nusrat / Alrbyawi, Hamad / Al-Anazy, Murefah Mana / Elkaeed, Eslam B / Somaily, H H / Pashameah, Rami Adel / Alzahrani, Eman / Farouk, Abd-ElAziem

    Molecules (Basel, Switzerland)

    2022  Volume 27, Issue 19

    Abstract: By using the chemical bath deposition approach, binary bismuth sulphides ( ... ...

    Abstract By using the chemical bath deposition approach, binary bismuth sulphides (Bi
    Language English
    Publishing date 2022-09-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules27196419
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    Zs.MO 81: Show issues

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  8. Article ; Online: Fabrication of a Ternary Nanocomposite g-C

    Afzal, Malik Imran / Shahid, Sammia / Mansoor, Sana / Javed, Mohsin / Iqbal, Shahid / Hakami, Othman / Yousef, El Sayed / Al-Fawzan, Foziah F / Elkaeed, Eslam B / Pashameah, Rami Adel / Alzahrani, Eman / Farouk, Abd-ElAziem

    Toxics

    2022  Volume 10, Issue 11

    Abstract: The synthesis of a photo-catalyst with a narrow bandgap and efficient capability to degrade contaminants in the presence of sunlight is currently challenging but exciting. In this work, an efficient photocatalytic ternary nanocomposite g- ... ...

    Abstract The synthesis of a photo-catalyst with a narrow bandgap and efficient capability to degrade contaminants in the presence of sunlight is currently challenging but exciting. In this work, an efficient photocatalytic ternary nanocomposite g-C
    Language English
    Publishing date 2022-10-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2733883-6
    ISSN 2305-6304 ; 2305-6304
    ISSN (online) 2305-6304
    ISSN 2305-6304
    DOI 10.3390/toxics10110657
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  9. Article: Boosted spatial charge carrier separation of binary ZnFe

    Iqbal, Shahid / Amjad, Adnan / Javed, Mohsin / Alfakeer, M / Mushtaq, Muhammad / Rabea, Sameh / Elkaeed, Eslam B / Pashameah, Rami Adel / Alzahrani, Eman / Farouk, Abd-ElAziem

    Frontiers in chemistry

    2022  Volume 10, Page(s) 975355

    Abstract: A potential method for removing toxins from contaminated wastewater, especially organic pollutants, is photo-catalysis. Here, a simple technique for producing zinc ferrite nanoparticles ( ... ...

    Abstract A potential method for removing toxins from contaminated wastewater, especially organic pollutants, is photo-catalysis. Here, a simple technique for producing zinc ferrite nanoparticles (ZnFe
    Language English
    Publishing date 2022-08-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2711776-5
    ISSN 2296-2646
    ISSN 2296-2646
    DOI 10.3389/fchem.2022.975355
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  10. Article: Design, Synthesis, In Silico Testing, and In Vitro Evaluation of Thiazolidinone-Based Benzothiazole Derivatives as Inhibitors of α-Amylase and α-Glucosidase.

    Khan, Shoaib / Iqbal, Shahid / Khan, Marwa / Rehman, Wajid / Shah, Mazloom / Hussain, Rafaqat / Rasheed, Liaqat / Khan, Yousaf / Dera, Ayed A / Pashameah, Rami Adel / Alzahrani, Eman / Farouk, Abd-ElAziem

    Pharmaceuticals (Basel, Switzerland)

    2022  Volume 15, Issue 10

    Abstract: In this study, a stepwise reaction afforded thiazolidinone-based benzothiazole ... ...

    Abstract In this study, a stepwise reaction afforded thiazolidinone-based benzothiazole derivatives
    Language English
    Publishing date 2022-09-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2193542-7
    ISSN 1424-8247
    ISSN 1424-8247
    DOI 10.3390/ph15101164
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