LIVIVO - Search results -

Search results

Result 1 - 10 of total 44

Search options

Article: OCT4 induces long-lived dedifferentiated kidney progenitors poised to redifferentiate in 3D kidney spheroids.

Omer, Dorit / Zontag, Osnat Cohen / Gnatek, Yehudit / Harari-Steinberg, Orit / Pleniceanu, Oren / Namestnikov, Michael / Cohen, Ayelet-Hashahar / Nissim-Rafinia, Malka / Tam, Gal / Kalisky, Tomer / Meshorer, Eran / Dekel, Benjamin

Molecular therapy. Methods & clinical development

2023 Volume 29, Page(s) 329–346

Abstract: Upscaling of kidney epithelial cells is crucial for renal regenerative medicine. Nonetheless, the adult kidney lacks a distinct stem cell hierarchy, limiting the ability to long-term propagate clonal populations of primary cells that retain renal ... ...

Abstract	Upscaling of kidney epithelial cells is crucial for renal regenerative medicine. Nonetheless, the adult kidney lacks a distinct stem cell hierarchy, limiting the ability to long-term propagate clonal populations of primary cells that retain renal identity. Toward this goal, we tested the paradigm of shifting the balance between differentiation and stemness in the kidney by introducing a single pluripotency factor, OCT4. Here we show that ectopic expression of OCT4 in human adult kidney epithelial cells (hKEpC) induces the cells to dedifferentiate, stably proliferate, and clonally emerge over many generations. Control hKEpC dedifferentiate, assume fibroblastic morphology, and completely lose clonogenic capacity. Analysis of gene expression and histone methylation patterns revealed that OCT4 represses the HNF1B gene module, which is critical for kidney epithelial differentiation, and concomitantly activates stemness-related pathways. OCT4-hKEpC can be long-term expanded in the dedifferentiated state that is primed for renal differentiation. Thus, when expanded OCT4-hKEpC are grown as kidney spheroids (OCT4-kSPH), they reactivate the HNF1B gene signature, redifferentiate, and efficiently generate renal structures
Language	English
Publishing date	2023-04-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	2872938-9
ISSN	2329-0501 ; 2329-0501
ISSN (online)	2329-0501
ISSN	2329-0501
DOI	10.1016/j.omtm.2023.04.005
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 7107: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: The Feasibility to Isolate and Expand Tympanic Membrane Squamous Epithelium Stem Cells From Scarred Perforation Margins.

Sagiv, Doron / Harari-Steinberg, Orit / Wolf, Michael / Dekel, Benjamin / Omer, Dorit

Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology

2019 Volume 40, Issue 10, Page(s) e1030–e1036

Abstract: Hypothesis: The scarred rim of chronic tympanic membrane (TM) perforation contains keratinocytes with potential for regeneration while maintaining their morphological and genetic characteristics.: Background: The squamous epithelium of the TM has a ... ...

Abstract	Hypothesis: The scarred rim of chronic tympanic membrane (TM) perforation contains keratinocytes with potential for regeneration while maintaining their morphological and genetic characteristics. Background: The squamous epithelium of the TM has a good regeneration capacity. Successful isolation and expansion of human TM keratinocytes (hTMKR) was reported from a full, en-bloc, healthy TM. Methods: Trimmed margins of the TM perforation (harvested during tympanoplasty) underwent enzymatic digestion (collagenase or trypsin) and were seeded either with serum-containing medium (SCM) or keratinocyte serum-free medium (KSFM) and progenitor cell growth medium (PR) (KSFM:PR, 1:1). Gene expression analysis by real-time qRT-PCR was used to compare between human TM cells derived from scarred perforation margins (hTMKR), normal human skin keratinocytes (NhSKR), and human fibroblasts. Results: Twelve patients were included in the study. In 9 of 12 cases (75%) single-cell isolation with fibroblastic or epithelial cell morphology (or both) was achieved. Cells seeded with KSFM:PR yielded epithelial morphology (hTMKR) while SCM culturing resulted in a fibroblastic morphology (hTMFib). Gene expression analysis revealed significant higher expression of VCAN (p = 0.002) and FOXC2 (p = 0.015) at the mRNA levels (normal hTMKR markers) in hTMKR compared to NhSKR. In addition, a comparison of gene expression between hTMKR and hTMFib revealed significantly higher levels of both VCAN (p = 0.045) and SLC6A14 (p = 0.036) among hTMKR. Conclusion: For the first time, we developed a protocol to isolate hTMKR from scarred TM perforation margins. Furthermore, we succeeded in achieving tissue expansion that preserved the characteristic of healthy TM cells. This study bridges "regenerative medicine" approach with clinical and surgical objectives.
MeSH term(s)	Adolescent ; Adult ; Aged ; Amino Acid Transport Systems ; Cell Culture Techniques/methods ; Child ; Cicatrix/pathology ; Cicatrix/surgery ; Epithelial Cells/cytology ; Feasibility Studies ; Female ; Fibroblasts ; Humans ; Keratinocytes/cytology ; Male ; Middle Aged ; Regenerative Medicine/methods ; Stem Cells/cytology ; Tissue Culture Techniques/methods ; Tissue and Organ Harvesting/methods ; Tympanic Membrane/cytology ; Tympanic Membrane/pathology ; Tympanic Membrane Perforation/complications ; Tympanoplasty/methods ; Young Adult
Chemical Substances	Amino Acid Transport Systems ; SLC6A14 protein, human
Language	English
Publishing date	2019-08-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	2036790-9
ISSN	1537-4505 ; 1531-7129
ISSN (online)	1537-4505
ISSN	1531-7129
DOI	10.1097/MAO.0000000000002367
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1554: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Renal lineage cells as a source for renal regeneration.

Pleniceanu, Oren / Omer, Dorit / Harari-Steinberg, Orit / Dekel, Benjamin

Pediatric research

2017 Volume 83, Issue 1-2, Page(s) 267–274

Abstract: The mammalian kidney is a highly complex organ, composed of various cell types within a unique structural framework. Nonetheless, in recent years, giant leaps in our understanding of nephrogenesis and the origin of new cells in the adult kidney have ... ...

Abstract	The mammalian kidney is a highly complex organ, composed of various cell types within a unique structural framework. Nonetheless, in recent years, giant leaps in our understanding of nephrogenesis and the origin of new cells in the adult kidney have resulted in novel routes to regenerate damaged nephrons. While several strategies can be envisioned to achieve this aim, one common theme is the reliance on renal lineage cells, as extrarenal cells, such as bone marrow-derived cells, have been shown to be devoid of renal differentiation capacity. Herein, we will present the main motivation for the pursuit for cell-based therapies, which is the ever growing problem of chronic kidney disease (CKD), and discuss different strategies toward replenishing the damaged renal parenchyma. These include transplantation of fetal kidney grafts or fetal kidney stem cells, directed differentiation of pluripotent stem cells into kidney epithelia, establishment of renal progenitors from the adult kidney, and genetic reprogramming of mature kidney cells into a progenitor state. Taken together with novel techniques recapitulating the three-dimensional developmental environment, these advances are expected to take the field into a new era, bringing us closer than ever to the day when kidney stem cell-based therapy becomes a viable therapeutic option.
MeSH term(s)	Animals ; Cell Differentiation ; Cell Lineage ; Epithelial Cells/cytology ; Homeostasis ; Humans ; Kidney/cytology ; Kidney Failure, Chronic/therapy ; Kidney Transplantation ; Mice ; Nephrons/metabolism ; Organogenesis ; Pluripotent Stem Cells/cytology ; Quality of Life ; Regeneration ; Regenerative Medicine ; Stem Cell Transplantation ; Stem Cells/cytology
Language	English
Publishing date	2017-11-15
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	4411-8
ISSN	1530-0447 ; 0031-3998
ISSN (online)	1530-0447
ISSN	0031-3998
DOI	10.1038/pr.2017.255
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 564: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 373: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Author Correction: Human kidney clonal proliferation disclose lineage-restricted precursor characteristics.

Cohen-Zontag, Osnat / Gershon, Rotem / Harari-Steinberg, Orit / Kanter, Itamar / Omer, Dorit / Pleniceanu, Oren / Tam, Gal / Oriel, Sarit / Ben-Hur, Herzl / Katz, Guy / Dotan, Zohar / Kalisky, Tomer / Dekel, Benjamin / Pode-Shakked, Naomi

Scientific reports

2021 Volume 11, Issue 1, Page(s) 6970

Language	English
Publishing date	2021-03-22
Publishing country	England
Document type	Published Erratum
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-86157-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: mTORC1 Inhibition Is an Effective Treatment for Sporadic Renal Angiomyolipoma.

Pleniceanu, Oren / Omer, Dorit / Azaria, Einat / Harari-Steinberg, Orit / Dekel, Benjamin

Kidney international reports

2017 Volume 3, Issue 1, Page(s) 155–159

Abstract: Introduction: Renal angiomyolipoma (AML) is the most common benign renal tumor. Despite a generally benign histology, AML can result in significant morbidity, from intra-abdominal hemorrhage and reduction in kidney function. While classically associated ...

Abstract	Introduction: Renal angiomyolipoma (AML) is the most common benign renal tumor. Despite a generally benign histology, AML can result in significant morbidity, from intra-abdominal hemorrhage and reduction in kidney function. While classically associated with the autosomal dominant disorder tuberous sclerosis complex (TSC) or with pulmonary lymphangioleiomyomatosis, most AMLs are sporadic. Mammalian target of rapamycin complex 1 (mTORC1) inhibitors (e.g., sirolimus) have been found to be effective in treating TSC- or lymphangioleiomyomatosis-associated AML, but to date it is unknown whether this strategy is effective for sporadic AML. Methods: We stained tumor specimens of sporadic AML patients for pS6 to assess for mTORC1 activation. Results: We detected strong activation of the mTORC1 pathway, similar to TSC-associated AML. Consequently, we showed that Conclusion: We propose mTORC1 inhibition as an effective treatment option for patients with sporadic AML, which represents the vast majority of patients with this tumor.
Language	English
Publishing date	2017-08-12
Publishing country	United States
Document type	Journal Article
ISSN	2468-0249
ISSN (online)	2468-0249
DOI	10.1016/j.ekir.2017.07.016
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Blastemal NCAM

Raved, Dani / Tokatly-Latzer, Itay / Anafi, Liat / Harari-Steinberg, Orit / Barshack, Iris / Dekel, Benjamin / Pode-Shakked, Naomi

Pathology, research and practice

2019 Volume 215, Issue 8, Page(s) 152491

Abstract: Background: Cancer Stem Cells (CSCs) have been suggested as the culprit responsible for tumor resistance to treatment and disease recurrence. Wilms' tumor (WT) is a paradigm for studying the relation between development and tumorigenesis, showing three ... ...

Abstract	Background: Cancer Stem Cells (CSCs) have been suggested as the culprit responsible for tumor resistance to treatment and disease recurrence. Wilms' tumor (WT) is a paradigm for studying the relation between development and tumorigenesis, showing three main histological elements: undifferentiated blastema, epithelia and stroma, mimicking human kidney development. NCAM + ALDH1+ cells were previously found to contain the cancer stem like-cell population in WT. Thus far, the correlation between histologic characterization of this cell population, clinicopathologic parameters and prognostic outcome has yet been investigated in WT. Procedures: Paraffin-imbedded primary WT specimens from twenty-four patients were immunostained for NCAM and ALDH1. Positivity and histologic compartment localization were determined by two independent observers, blinded to the clinical outcome. Clinicopathologic parameters and prognostic outcomes were determined based on the patients' medical records. The association of NCAM and ALDH1 co-localization with clinicopathologic characteristics was analyzed byχ Results: Blastemal co-localization of NCAM and ALDH1 was observed in 33% of WTs. Metastases, ICE chemotherapy protocol, blastemal predominance following preoperative chemotherapy, recurrence and patient demise were found to significantly correlate with blastemal NCAM + ALDH1+ cell staining (p < 0.05). A significant inverse correlation between blastemal double positive cells, disease-free survival and overall survival was also observed. Conclusions: WT blastemal NCAM + ALDH1+ CSCs significantly correlate with adverse clinicopathologic parameters and poorer prognosis. These results underscore the role of CSCs in disease progression. Additionally, this pilot study supports the addition of these markers for risk stratification of WTs.
MeSH term(s)	Antineoplastic Combined Chemotherapy Protocols ; Child, Preschool ; Disease Progression ; Disease-Free Survival ; Female ; Humans ; Kidney/pathology ; Kidney Neoplasms/pathology ; Kidney Neoplasms/therapy ; Male ; Neoplasm Recurrence, Local/pathology ; Neoplasm Recurrence, Local/therapy ; Neoplastic Stem Cells/pathology ; Neural Cell Adhesion Molecules/metabolism ; Pilot Projects ; Treatment Outcome ; Wilms Tumor/pathology ; Wilms Tumor/therapy
Chemical Substances	Neural Cell Adhesion Molecules
Language	English
Publishing date	2019-06-10
Publishing country	Germany
Document type	Journal Article
ZDB-ID	391889-0
ISSN	1618-0631 ; 0344-0338
ISSN (online)	1618-0631
ISSN	0344-0338
DOI	10.1016/j.prp.2019.152491
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ud III Zs.20: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Author Correction

Osnat Cohen-Zontag / Rotem Gershon / Orit Harari-Steinberg / Itamar Kanter / Dorit Omer / Oren Pleniceanu / Gal Tam / Sarit Oriel / Herzl Ben-Hur / Guy Katz / Zohar Dotan / Tomer Kalisky / Benjamin Dekel / Naomi Pode-Shakked

Scientific Reports, Vol 11, Iss 1, Pp 1-

Human kidney clonal proliferation disclose lineage-restricted precursor characteristics

2021 Volume 1

Abstract: An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

Abstract	An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2021-03-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Human kidney clonal proliferation disclose lineage-restricted precursor characteristics.

Cohen-Zontag, Osnat / Gershon, Rotem / Harari-Steinberg, Orit / Kanter, Itamar / Omer, Dorit / Pleniceanu, Oren / Tam, Gal / Oriel, Sarit / Ben-Hur, Herzel / Katz, Guy / Dotan, Zohar / Kalisky, Tomer / Dekel, Benjamin / Pode-Shakked, Naomi

Scientific reports

2020 Volume 10, Issue 1, Page(s) 22097

Abstract: In-vivo single cell clonal analysis in the adult mouse kidney has previously shown lineage-restricted clonal proliferation within varying nephron segments as a mechanism responsible for cell replacement and local regeneration. To analyze ex-vivo clonal ... ...

Abstract	In-vivo single cell clonal analysis in the adult mouse kidney has previously shown lineage-restricted clonal proliferation within varying nephron segments as a mechanism responsible for cell replacement and local regeneration. To analyze ex-vivo clonal growth, we now preformed limiting dilution to generate genuine clonal cultures from one single human renal epithelial cell, which can give rise to up to 3.4 * 10
MeSH term(s)	Cell Differentiation/genetics ; Cell Proliferation/genetics ; Clonal Evolution/genetics ; Computational Biology ; Epithelial Cells/cytology ; Epithelial-Mesenchymal Transition/genetics ; Humans ; Kidney/cytology ; Kidney/growth & development ; Mesoderm/growth & development ; Mesoderm/metabolism ; Nephrons/growth & development ; Nephrons/metabolism ; Primary Cell Culture ; Regeneration/genetics ; Single-Cell Analysis ; Stem Cells/cytology
Language	English
Publishing date	2020-12-16
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-78366-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Human-engineered auricular reconstruction (hEAR) by 3D-printed molding with human-derived auricular and costal chondrocytes and adipose-derived mesenchymal stem cells.

Landau, Shira / Szklanny, Ariel A / Machour, Majd / Kaplan, Ben / Shandalov, Yulia / Redenski, Idan / Beckerman, Margarita / Harari-Steinberg, Orit / Zavin, Janet / Karni-Katovitch, Oryan / Goldfracht, Idit / Michael, Inbal / Waldman, Stephen D / Duvdevani, Shay I / Levenberg, Shulamit

Biofabrication

2021 Volume 14, Issue 1

Abstract: Microtia is a small, malformed external ear, which occurs at an incidence of 1-10 per 10 000 births. Autologous reconstruction using costal cartilage is the most widely accepted surgical microtia repair technique. Yet, the method involves donor-site pain ...

Abstract	Microtia is a small, malformed external ear, which occurs at an incidence of 1-10 per 10 000 births. Autologous reconstruction using costal cartilage is the most widely accepted surgical microtia repair technique. Yet, the method involves donor-site pain and discomfort and relies on the artistic skill of the surgeon to create an aesthetic ear. This study employed novel tissue engineering techniques to overcome these limitations by developing a clinical-grade, 3D-printed biodegradable auricle scaffold that formed stable, custom-made neocartilage implants. The unique scaffold design combined strategically reinforced areas to maintain the complex topography of the outer ear and micropores to allow cell adhesion for the effective production of stable cartilage. The auricle construct was computed tomography (CT) scan-based composed of a 3D-printed clinical-grade polycaprolactone scaffold loaded with patient-derived chondrocytes produced from either auricular cartilage or costal cartilage biopsies combined with adipose-derived mesenchymal stem cells. Cartilage formation was measured within the construct
MeSH term(s)	Animals ; Chondrocytes ; Congenital Microtia/surgery ; Ear Cartilage ; Humans ; Mesenchymal Stem Cells ; Mice ; Printing, Three-Dimensional ; Tissue Engineering/methods ; Tissue Scaffolds
Language	English
Publishing date	2021-12-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2500944-8
ISSN	1758-5090 ; 1758-5082
ISSN (online)	1758-5090
ISSN	1758-5082
DOI	10.1088/1758-5090/ac3b91
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Selecting the optimal cell for kidney regeneration: fetal, adult or reprogrammed stem cells.

Harari-Steinberg, Orit / Pleniceanu, Oren / Dekel, Benjamin

Organogenesis

2011 Volume 7, Issue 2, Page(s) 123–134

Abstract: Chronic kidney disease (CKD) is a progressive loss in renal function over a period of months or years. End-stage renal disease (ESRD) or stage 5 CKD ensues when renal function deteriorates to under 15% of the normal range. ESRD requires either dialysis ... ...

Abstract	Chronic kidney disease (CKD) is a progressive loss in renal function over a period of months or years. End-stage renal disease (ESRD) or stage 5 CKD ensues when renal function deteriorates to under 15% of the normal range. ESRD requires either dialysis or, preferentially, a kidney organ allograft, which is severely limited due to organ shortage for transplantation. To combat this situation, one needs to either increase supply of organs or decrease their demand. Two strategies therefore exist: for those that have completely lost their kidney function (ESRD), we will need to supply new kidneys. Taking into account the kidneys' extremely complex structure, this may prove to be impossible in the near future. In contrast, for those patients that are in the slow progression route from CKD to ESRD but still have functional kidneys, we might be able to halt progression by introducing stem cell therapy to diseased kidneys to rejuvenate or regenerate individual cell types. Multiple cell compartments that fall into three categories are likely to be worthy targets for cell repair: vessels, stroma (interstitium) and nephron epithelia. Different stem/progenitor cells can be linked to regeneration of specific cell types; hematopoietic progenitors and hemangioblastic cell types have specific effects on the vascular niche (vasculogenesis and angiogenesis). Multipotent stromal cells (MSC), whether derived from the bone marrow or isolated from the kidney's non-tubular compartment, may, in turn, heal nephron epithelia via paracrine mechanisms. Nevertheless, as we now know that all of the above lack nephrogenic potential, we should continue our quest to derive genuine nephron (epithelial) progenitors from differentiated pluripotent stem cells, from fetal and adult kidneys and from directly reprogrammed somatic cells.
MeSH term(s)	Adult Stem Cells/cytology ; Animals ; Cellular Reprogramming ; Fetal Stem Cells/cytology ; Humans ; Kidney/physiology ; Multipotent Stem Cells/cytology ; Regeneration/physiology
Language	English
Publishing date	2011-04-01
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2159583-5
ISSN	1555-8592 ; 1555-8592
ISSN (online)	1555-8592
ISSN	1555-8592
DOI	10.4161/org.7.2.15783
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito