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  1. Article ; Online: Photoinactivation by UVA radiation and visible light of Candida auris compared to other fungi.

    Gierke, Anna-Maria / Hessling, Martin

    Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

    2024  Volume 23, Issue 4, Page(s) 681–692

    Abstract: In addition to the rising number of patients affected by viruses and bacteria, the number of fungal infections has also been rising over the years. Due to the increase in resistance to various antimycotics, investigations into further disinfection ... ...

    Abstract In addition to the rising number of patients affected by viruses and bacteria, the number of fungal infections has also been rising over the years. Due to the increase in resistance to various antimycotics, investigations into further disinfection options are important. In this study, two yeasts (Candida auris and Saccharomyces cerevisiae) and a mold (Cladosporium cladosporioides) were irradiated at 365, 400, and 450 nm individually. The resulting log 1 reduction doses were determined and compared with other studies. Furthermore, fluorescence measurements of C. auris were performed to detect possible involved photosensitizers. A roughly exponential photoinactivation was observed for all three fungi and all irradiation wavelengths with higher D90 doses for longer wavelengths. The determined log 1 reduction doses of C. auris and S. cerevisiae converged with increasing wavelength. However, S. cerevisiae was more photosensitive than C. auris for all irradiation wavelengths and is therefore not a suitable C. auris surrogate for photoinactivation experiments. For the mold C. cladosporioides, much higher D90 doses were determined than for both yeasts. Concerning potential photosensitizers, flavins and various porphyrins were detected by fluorescence measurements. By excitation at 365 nm, another, so far unreported fluorophore and potential photosensitizer was also observed. Based on its fluorescence spectrum, we assume it to be thiamine.Graphic abstract.
    MeSH term(s) Humans ; Candida auris ; Saccharomyces cerevisiae ; Photosensitizing Agents/pharmacology ; Light ; Ultraviolet Rays ; Antifungal Agents ; Microbial Sensitivity Tests
    Chemical Substances Photosensitizing Agents ; Antifungal Agents
    Language English
    Publishing date 2024-03-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 2072584-X
    ISSN 1474-9092 ; 1474-905X
    ISSN (online) 1474-9092
    ISSN 1474-905X
    DOI 10.1007/s43630-024-00543-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Determination of Correlated Color Temperature in Ex Vivo Porcine Eyes during Intraocular Illumination.

    Fehler, Nicole / Hessling, Martin

    Journal of clinical medicine

    2023  Volume 12, Issue 8

    Abstract: 1) Background: In ophthalmic surgery, white light is mostly applied to illuminate the intraocular space, and ophthalmologists are comfortable working with it. Diaphanoscopic illumination changes the spectral composition of light, resulting in a change ... ...

    Abstract (1) Background: In ophthalmic surgery, white light is mostly applied to illuminate the intraocular space, and ophthalmologists are comfortable working with it. Diaphanoscopic illumination changes the spectral composition of light, resulting in a change in the correlated color temperature (CCT) of the intraocular illumination. This color change makes it difficult for surgeons to recognize the structures in the eye. CCT during intraocular illumination has not yet been measured before, and it is the aim of this study to perform such measurement. (2) Methods: CCT was measured inside ex vivo porcine eyes during diaphanoscopic illumination and endoillumination using a current ophthalmic illumination system with a detection fiber inside the eye. By applying pressure on the eye with a diaphanoscopic fiber, the dependency of CCT on pressure was examined. (3) Results: The intraocular CCT values during endoillumination were 3923 K and 5407 K for the halogen and xenon lamps, respectively. During diaphanoscopic illumination, a strong unwanted red shift was observed, resulting in 2199 K and 2675 K for the xenon and the halogen lamps, respectively. Regarding different applied pressures, the CCT did not differ considerably. (4) Conclusions: This red shift should be compensated for in the development of new illumination systems since surgeons are used to white light illumination, which also simplifies the identification of retinal structures.
    Language English
    Publishing date 2023-04-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm12083034
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  3. Article ; Online: Photoinactivation of the bacteriophage PhiX174 by UVA radiation and visible light in SM buffer and DMEM-F12.

    Sommerfeld, Florian / Weyersberg, Laura / Vatter, Petra / Hessling, Martin

    BMC research notes

    2024  Volume 17, Issue 1, Page(s) 3

    Abstract: Objective: It has been observed that viruses can be inactivated by UVA radiation and visible light. The aim of this study is to investigate whether a medium that contains a photosensitizer might have an influence on viral reduction under irradiation by ... ...

    Abstract Objective: It has been observed that viruses can be inactivated by UVA radiation and visible light. The aim of this study is to investigate whether a medium that contains a photosensitizer might have an influence on viral reduction under irradiation by UVA, violet or blue light. Test virus is the bacteriophage PhiX174 in the photosensitizer-free SM buffer and DMEM-F12, which contains the known photosensitizer riboflavin.
    Results: The determined PhiX174 D90 doses in SM buffer and DMEM were 36.8 J/cm² and 13.6 J/cm² at 366 nm, 153.6 J/cm² and 129.1 J/cm² at 408 nm and 4988 J/cm² and 2477.1 J/cm² at 455 nm, respectively. It can be concluded that the medium has a large influence on the results. This might be caused by the photosensitizer riboflavin in DMEM-F12. As riboflavin is a key component in many cell culture media, irradiation experiments with viruses in cell culture media should be avoided if the investigation of intrinsical photoinactivation properties of viruses is aimed for.
    MeSH term(s) Photosensitizing Agents/pharmacology ; Bacteriophages ; Light ; Ultraviolet Rays ; Riboflavin/pharmacology
    Chemical Substances Photosensitizing Agents ; Riboflavin (TLM2976OFR)
    Language English
    Publishing date 2024-01-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 2413336-X
    ISSN 1756-0500 ; 1756-0500
    ISSN (online) 1756-0500
    ISSN 1756-0500
    DOI 10.1186/s13104-023-06658-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Investigation on Potential ESKAPE Surrogates for 222 and 254 nm Irradiation Experiments.

    Gierke, Anna-Maria / Hessling, Martin

    Frontiers in microbiology

    2022  Volume 13, Page(s) 942708

    Abstract: Background: Due to the increase in multidrug-resistant pathogens, it is important to investigate further antimicrobial options. In order not to have to work directly with pathogens, the investigation of possible surrogates is an important aspect. It is ... ...

    Abstract Background: Due to the increase in multidrug-resistant pathogens, it is important to investigate further antimicrobial options. In order not to have to work directly with pathogens, the investigation of possible surrogates is an important aspect. It is examined how suitable possible surrogate candidates for ESKAPE pathogens are for UVC applications. In addition, the inactivation sensitivities to 222 and 254 nm radiation are compared in relation.
    Methods: Non-pathogenic members (
    Results: For all non-pathogen relatives (except
    Conclusion: For more than half of the studied cases, the examined ESKAPE relatives in this study can be applied as surrogates for ESKAPE pathogens. Because of lack of data, no clear statement could be made for
    Language English
    Publishing date 2022-07-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.942708
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Confinement-enhanced Li

    Hessling, Janis / Lange, Martin / Schönhoff, Monika

    Physical chemistry chemical physics : PCCP

    2023  Volume 25, Issue 35, Page(s) 23510–23518

    Abstract: While Ionic Liquids (IL) are promising liquid electrolyte components for Li-ion batteries due to their high electrochemical stability and low volatility and flammability, unfavorable Lithium-anion clusters lead to poor ... ...

    Abstract While Ionic Liquids (IL) are promising liquid electrolyte components for Li-ion batteries due to their high electrochemical stability and low volatility and flammability, unfavorable Lithium-anion clusters lead to poor Li
    Language English
    Publishing date 2023-09-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 1476244-4
    ISSN 1463-9084 ; 1463-9076
    ISSN (online) 1463-9084
    ISSN 1463-9076
    DOI 10.1039/d3cp02901h
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  6. Article: Far-UVC Radiation for Disinfecting Hands or Gloves?

    Hessling, Martin / Sicks, Ben / Lau, Bernhard

    Pathogens (Basel, Switzerland)

    2023  Volume 12, Issue 2

    Abstract: 1) Background: Far-UVC radiation in the spectral range 200-230 nm has, according to previous findings, a strong antimicrobial effect on pathogens, but exhibits hardly any harmful effect on human skin. Therefore, the present study will discuss whether ... ...

    Abstract (1) Background: Far-UVC radiation in the spectral range 200-230 nm has, according to previous findings, a strong antimicrobial effect on pathogens, but exhibits hardly any harmful effect on human skin. Therefore, the present study will discuss whether such radiation could also be suitable for hand disinfection in the healthcare sector. (2) Methods: Hands and gloves were microbially contaminated and exposed to radiation from a 222 nm krypton-chloride-excimer lamp. The applied doses were 23 mJ/cm
    Language English
    Publishing date 2023-01-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens12020213
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  7. Article ; Online: Cataract Development by Exposure to Ultraviolet and Blue Visible Light in Porcine Lenses.

    Haag, Robin / Sieber, Nicole / Heßling, Martin

    Medicina (Kaunas, Lithuania)

    2021  Volume 57, Issue 6

    Abstract: Background and ... ...

    Abstract Background and Objectives
    MeSH term(s) Animals ; Cataract/etiology ; Lens, Crystalline ; Light ; Swine ; Ultraviolet Rays/adverse effects
    Language English
    Publishing date 2021-05-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2188113-3
    ISSN 1648-9144 ; 1010-660X
    ISSN (online) 1648-9144
    ISSN 1010-660X
    DOI 10.3390/medicina57060535
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6270: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  8. Article ; Online: Photoinactivation of the bacteriophage PhiX174 by UVA radiation and visible light in SM buffer and DMEM-F12

    Florian Sommerfeld / Laura Weyersberg / Petra Vatter / Martin Hessling

    BMC Research Notes, Vol 17, Iss 1, Pp 1-

    2024  Volume 7

    Abstract: Abstract Objective It has been observed that viruses can be inactivated by UVA radiation and visible light. The aim of this study is to investigate whether a medium that contains a photosensitizer might have an influence on viral reduction under ... ...

    Abstract Abstract Objective It has been observed that viruses can be inactivated by UVA radiation and visible light. The aim of this study is to investigate whether a medium that contains a photosensitizer might have an influence on viral reduction under irradiation by UVA, violet or blue light. Test virus is the bacteriophage PhiX174 in the photosensitizer-free SM buffer and DMEM-F12, which contains the known photosensitizer riboflavin. Results The determined PhiX174 D90 doses in SM buffer and DMEM were 36.8 J/cm² and 13.6 J/cm² at 366 nm, 153.6 J/cm² and 129.1 J/cm² at 408 nm and 4988 J/cm² and 2477.1 J/cm² at 455 nm, respectively. It can be concluded that the medium has a large influence on the results. This might be caused by the photosensitizer riboflavin in DMEM-F12. As riboflavin is a key component in many cell culture media, irradiation experiments with viruses in cell culture media should be avoided if the investigation of intrinsical photoinactivation properties of viruses is aimed for.
    Keywords PhiX174 ; UVA ; Visible irradiation ; Radiation inactivation ; Blue light ; Violet light ; Medicine ; R ; Biology (General) ; QH301-705.5 ; Science (General) ; Q1-390
    Subject code 535
    Language English
    Publishing date 2024-01-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: UV radiation sensitivity of bacteriophage PhiX174 - A potential surrogate for SARS-CoV-2 in terms of radiation inactivation.

    Weyersberg, Laura / Sommerfeld, Florian / Vatter, Petra / Hessling, Martin

    AIMS microbiology

    2023  Volume 9, Issue 3, Page(s) 431–443

    Abstract: To minimize health risks, surrogates are often employed to reduce experiments with pathogenic microorganisms and the associated health risk. Due to structural similarities between the enveloped RNA-viruses SARS-CoV-2 and Phi6, the latter has been ... ...

    Abstract To minimize health risks, surrogates are often employed to reduce experiments with pathogenic microorganisms and the associated health risk. Due to structural similarities between the enveloped RNA-viruses SARS-CoV-2 and Phi6, the latter has been established as a nonpathogenic coronavirus surrogate for many applications. However, large discrepancies in the UV log-reduction doses between SARS-CoV-2 and Phi6 necessitate the search for a better surrogate for UV inactivation applications. A literature study provided the bacteriophage PhiX174 as a potentially more suitable nonpathogenic coronavirus surrogate candidate. In irradiation experiments, the sensitivity of PhiX174 was investigated upon exposure to UV radiation of wavelengths 222 nm (Far-UVC), 254 nm (UVC), 302 nm (broad-band UVB), 311 nm (narrow-band UVB) and 366 nm (UVA) using a plaque assay. The determined log-reduction doses for PhiX174 were 1.3 mJ/cm
    Language English
    Publishing date 2023-05-05
    Publishing country United States
    Document type Journal Article
    ISSN 2471-1888
    ISSN (online) 2471-1888
    DOI 10.3934/microbiol.2023023
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Advancement of a RGBW-LED pen for diaphanoscopic illumination with adjustable color and intensity with tests on ex-vivo porcine eyes in terms of retinal risk and correlated color temperature.

    Fehler, Nicole / Schneider, David / Hessling, Martin

    Biomedical engineering letters

    2023  Volume 14, Issue 1, Page(s) 115–126

    Abstract: Diaphanoscopic illumination has the disadvantage that the intraocular spectrum is red-shifted due to transmission properties of the eyewall. This red-shift should be counteracted as well as the retinal risk should be reduced with adjusting the spectral ... ...

    Abstract Diaphanoscopic illumination has the disadvantage that the intraocular spectrum is red-shifted due to transmission properties of the eyewall. This red-shift should be counteracted as well as the retinal risk should be reduced with adjusting the spectral distribution of the illumination light. Likewise, the illumination spectrum has to be adapted to the eye color of the patient. With the further development of a red, green, blue and white light-emitting diode (RGBW-LED) diaphanoscopy pen, the intensities of each color can be varied. The functionality of the LED pen is tested on ex-vivo porcine eyes. By measuring the transmission of the sclera and choroidea, the photochemical and thermal retinal hazard and the maximum exposure time are determined according to the standard DIN EN ISO 15004-2:2007. With this RGBW-LED pen the intraocular space can be illuminated clearly of up to 1.5 h without potential retinal damage according to DIN EN ISO 15004:2-2007. By adjusting the illumination spectrum the red-shift can be compensated and retinal risk can be reduced. By varying the LED intensities, the correlated color temperature in the eye can also be varied from cold white to warm white appearance as comfortable to the ophthalmologist. Additionally, a simple adjustment of the illumination to the eye color of the patient is possible. Using this RGBW-LED pen, the ophthalmologist can set the desired intraocular color appearance, which he prefers for special applications. He could also adjust the illumination to the eye color as this would reduce retinal hazard.
    Language English
    Publishing date 2023-09-15
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2602422-6
    ISSN 2093-985X ; 2093-9868
    ISSN (online) 2093-985X
    ISSN 2093-9868
    DOI 10.1007/s13534-023-00317-4
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